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Statistical Optimization of Solid Growth-medium for Rapid and Large Screening of Polysaccharides High-yielding Mycelial Cells of Inonotus obliquus (단백다당체 고생산성의 Inonotus obliquus 균주의 신속 개량을 위한 고체 성장배지의 통계적 최적화)

  • Hong, Hyung-Pyo;Jeong, Yong-Seob;Chun, Gie-Taek
    • KSBB Journal
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    • v.25 no.2
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    • pp.142-154
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    • 2010
  • The protein-bound innerpolysaccharides (IPS) produced by suspended mycelial cultures of Inonotus obliquus have promising potentials as an effective antidiabetic as well as an immunostimulating agents. To enhance IPS production, intensive strain improvement process should be carried out using large amount of UV-mutated protoplasts. During the whole strain-screening process, the stage of solid growth-culture was found to be the most time-requiring step, thus preventing rapid screening of high-yielding producers. In order to reduce the cell growth period in the solid growth-stage, therefore, solid growth-medium was optimized using the statistical methods such as (i) Plackett-Burman and fractional factorial designs (FFD) for selecting positive medium components, and (ii) steepest ascent (SAM) and response surface (RSM) methods for determining optimum concentrations of the selected components. By adopting the medium composition recommended by the SAM experiment, significantly higher growth rate was obtained in the solid growth-cultures, as represented by about 41% larger diameter of the cell growth circle and higher mycelial density. Sequential optimization process performed using the RSM experiments finally recommended the medium composition as follows: glucose 25.61g/L, brown rice 12.53 g/L, soytone peptone 12.53 g/L, $MgSO_4$ 5.53 g/L, and agar 20 g/L. It should be noted that this composition was almost similar to the medium combinations determined by the SAM experiment, demonstrating that the SAM was very helpful in finding out the final optimum concentrations. Through the use of this optimized medium, the period for the solid growth-culture could be successfully reduced to about 8 days from the previous 15~20 days, thus enabling large and mass screening of high producers in a relatively short period.

Ecophysiological Interpretations on the Water Relations Parameters of Trees(IX) - Measurement of the Transpiration Rate by the Heat Pulse Method in a Quercus mongolica Stand - (수목(樹木)의 수분특성(水分特性)에 관(關)한 생리(生理)·생태학적(生態學的) 해석(解析)(IX) - Heat pulse법(法)을 이용(利用)한 신갈나무임분(林分)의 증산속도(蒸散速度) 측정(測定) -)

  • Han, Sang Sup;Kim, Sun Hee
    • Journal of Korean Society of Forest Science
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    • v.85 no.2
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    • pp.288-299
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    • 1996
  • This is the basic study to investigate the amount of transpirational water loss in thrifty mature Quercus mongolica stand by the heat pulse method. The differences of heat pulse velocity by direction and depth, differences of heat pulse velocity by dominant, codominant and suppressed trees, diurnal changes of heat pulse velocity due to the change of leaf water potential, vapor pressure deficit and radiation, and sap flow path way in sapwood by dye penetration were measured in stems. Finally the amounts of daily and annual transpiration in stand were calculated by the heat pulse velocity. The results obtained were summarized as follows : 1. Relationship between heat pulse velocity(V) and sap flow rate(SFR) was obtained as a equation of SFR=1.37V. 2. The sap flow rate was high in the order of dominant, codominant, and suppressed trees. The daily heat pulse velocity changed with radiation, temperature and vapor pressure deficit. 3. The heat pulse velocity showed the similar diurnal variation as the leaf water potential change. 4. The heat pulse velocity showed the highest value in May(4.0cm/hr in average), the lowest one in July(2.9cm/hr in average). 5. The heat pulse velocity in the same stem presented the highest value in the northern direction, medium in western, and the lowest in southern and eastern. 6. The heat pulse velocity in stem was highest in 0.5cm, medium in 1.0cm, and lowest in 1.5cm depth from the surface of stem. 7. The sap flow path way in stem showed sectorial straight ascent pattern in four sample trees. 8. The amount of sap flow(SF) was presented as a equation of $SF=1.37A{\cdot}V$(A: the cross-sectional area of sapwood, V: heat pulse velocity), and especially SF was larger in dominant tree than codominant and suppressed trees. 9. The amount of daily transpiration was 5.6ton/ha/day, and its composition ratio was 72% at day and 28% at night. 10. The amount of stand transpiration per month was largest in May(168ton/ha/month), lowest in July(125ton/ha/month). The amount of stand transpiration per year was 839ton/ha/year.

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Mesothermal Gold Mineralization in the Boseong-Jangheung area, Chollanamdo-province (전라남도 보성-장흥지역의 중열수 금광화작용)

  • 허철호;윤성택;소칠섭
    • Economic and Environmental Geology
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    • v.35 no.5
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    • pp.379-393
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    • 2002
  • Within the Boseong-Jangheung area of Korea, five hydrothermal gold (-silver) quartz vein deposits occur. They have the characteristic features as follows: the relatively gold-rich nature of e1ectrurns; the absence of Ag-Sb( -As) sulfosalt mineral; the massive and simple mineralogy of veins. They suggest that gold mineralization in this area is correlated with late Jurassic to Early Cretaceous, mesothermal-type gold deposits in Korea. Fluid inclusion data show that fluid inclusions in stage I quartz of the mine area homogenize over a wide temperature range of 200$^{\circ}$ to 460$^{\circ}$C with salinities of 0.0 to 13.8 equiv. wt. % NaCI. The homogenization temperature of fluid inclusions in stage II calcite of the mine area ranges from 150$^{\circ}$ to 254$^{\circ}$C with salinities of 1.2 to 7.9 equiv. wt. % NaCI. This indicates a cooling of the hydrothermal fluid with time towards the waning of hydrothermal activity. Evidence of fluid boiling including CO2 effervescence indicates that pressures during entrapment of auriferous fluids in this area range up to 770 bars. Calculated sulfur isotope composition of auriferous fluids in this mine area (${\delta}^34S$_{{\Sigma}S}$$\textperthousand$) indicates an igneous source of sulfur in auriferous hydrothermal fluids. Within the Sobaegsan Massif, two representative mesothermal-type gold mine areas (Youngdong and Boseong-Jangheung areas) occur. The ${\delta}^34S values of sulfide minerals from Youngdong area range from -6.6 to 2.3$\textperthousand$ (average=-1.4$\textperthousand$, N=66), and those from BoseongJangheung area range from -0.7 to 3.6$\textperthousand$ (average=1.6$\textperthousand$, N=39). These i)34S values of both areas are comparatively lower than those of most Korean metallic ore deposits (3 to 7TEX>$\textperthousand$). And, within the Sobaegsan Massif, the ${\delta}^34S values of Youngdong area are lower than those of Boseong-Jangheung area. It is inferred that the difference of ${\delta}^34S values within the Sobaegsan Massif can be caused by either of the following mechanisms: (1) the presence of at least two distinct reservoirs (both igneous, with ${\delta}^34S values of < -6 $\textperthousand$ and 2$\pm$2 %0) for Jurassic mesothermal-type gold deposits in both areas; (2) different degrees of the mixing (assimilation) of 32S-enriched sulfur (possibly sulfur in Precambrian pelitic basement rocks) during the generation and/or subsequent ascent of magma; and/or (3) different degrees of the oxidation of an H2S-rich, magmatically derived sulfur source ${\delta}^34S = 2$\pm$2$\textperthousand$) during the ascent to mineralization sites. According to the observed differences in ore mineralogy (especially, iron-bearing ore minerals) and fluid inclusions of quartz from the mesothermal-type deposits in both areas, we conclude that pyrrhotite-rich, mesothermal-type deposits in the Youngdong area formed from higher temperatures and more reducing fluids than did pyrite(-arsenopyrite)-rich mesothermal-type deposits in the Boseong-Jangheung area. Therefore, we prefer the third mechanism than others because the ${\delta}^34S values of the Precambrian gneisses and Paleozoic sedimentary rocks occurring in both areas were not known to the present. In future, in order to elucidate the provenance of ore sulfur more systematically, we need to determine ${\delta}^34S values of the Precambrian metamorphic rocks and Paleozoic sedimentary rocks consisting the basement of the Korean Peninsula including the Sobaegsan Massif.

Evolution and Origin of the Geothermal Waters in the Busan Area, Korea: 1. Cooling and Dilution by Groundwater Mixing after Heated Seawater-Rock Interaction (부산지역 지열수의 기원과 진화: 1.가열된 해수-암석 반응과 지하수의 혼입에 따른 희석과 냉각)

  • 성규열;박맹언;고용권;김천수
    • Economic and Environmental Geology
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    • v.34 no.5
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    • pp.447-460
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    • 2001
  • The geothermal waters from the Busan area belong to Na-CI type and are characterized by much higher EC (921 ~6,520${\mu}$S/cm) and TDS (608-3,390 mg/L) than other geothermal waters in Korea. The concentration of majorions shows a weakly positive relationship with temperature except for Mg ion. The concentrations of the major cat ions have the order of Na>Ca>K>Mg. Ca ion is enriched and Mg ion is depleted compared with seawater. All Br concentrations of geothermal water are lower than those of seawater, showing a positive relationship with temperature. Generally geochemical characteristics of geothermal waters of the Busan area indicate that these waters have relatively increased Ca and Sr contents and depleted Mg, Na and K contents caused by seawater interaction with wall rock at depth during deep circulation of seawater. Base on the relationship between major ions and temperature, saline geothermal waters are diluted and are cooled by mixing of groundwaters during ascent. Isotope study and reaction path modeling of the overall geochemical system are required in order to better quantify the evolution and origin of geothermal waters in the Busan area.

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Fashion Changes in Subcultural Styles (2) -Focus on the Teddy Boys Style- (하위문화맥락에서 본 패션형태의 변화(2) -Teddy Boys를 중심으로-)

  • 양미경
    • Journal of the Korea Fashion and Costume Design Association
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    • v.4 no.1
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    • pp.61-72
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    • 2002
  • This paper is the second part of a series of the research about the Teddy boy style which is to examine various fashion changes in subcultural styles in 1900s. The main concern of this research is to investigate the creation and meaning of the Teddy boy style, how it interacted with the elements of class and generation and how the materials needed by the group constructed and appropriated into the visible systematic cultural form of correspondence. The Teddy Boys are the first recognized members of the British youth culture, which is known as the new Edwardian because of their dress. They had created the concept of the "teenagers," which forms the basis of the sense of a "generation" in the 20th century. The Teds set the style that would be used and modified in the following generations. They adopted the Edwardian style of the upper class, and changed it into their own style by modifying it and adding to it some other elements. The Teddy boys style is a special version of the sartorial appropriation encountered in the sphere of the fashion history. It actually began immediately after the war by the upper class youth far from the working class neighborhood. In the late 1953, the elitist aura surrounding the Edwardian suit was suddenly shattered. Within just a few months, the Edwardian suit became a source of social anxiety and the focus of a symbolic battle. Although the Edwardian look had initially went back to the upper class root, it became a symbol of rootlessness. In appropriation of this image, The Teddy boys were also rejecting the sartorial conformism of the English working class with its modest tradition. In this respect, the Teds effected the ascent or fall of the working class in the area of fashion. The Teds dress was not a merely borrowed fashion, but was a bastard fashion in the form of American trends, the Zoot suit. At this time members of the working class possessed only work dress for the week and waist suits for the Sunday outings. Teds broke this pattern, and developed the working class dandyism of wearing clothes simply to show off. The results were that they succeeded in opening the teen market, and popularizing a working class style for the first time in British history. The Teds became the first British street style with ties music, and remain as an symbol of the rising of a new age of values and styles.f values and styles.

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The Literatual Study on Pathologic Change Cognition to the Liver Disease (간장의 병리변화 인식에 대한 문헌적 고찰)

  • Lee Young Su;Kwack Jeong Jin;Lee Gang Nyoung;Choi Chang Won;Kim Hee Chul
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.4
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    • pp.630-636
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    • 2002
  • After The Yellow Emperor's Canon of Internal Medicine, The text researches of pathologic change to the liver disease concluded the next, 1, The category of liver-disease(肝病) include the Symptoms of abnormality due to vital energy and blood motion, emotion and intention, muscular and reproductive function, and legions around descending liver channel. 2. In the theory that Liver-Yang energy(肝陽氣) is always overproducing, Liver-Yin blood(肝陰血) is always lacking, pathologic characteristics for liver disease is functional change of malfunction of the use of body(體用失調), So nourishing the liver and kidney is used for the principal aspects of a disease. regulating and calm the liver is used for the secondary aspects of a disease as the treatment plan, 3. If malfunctioning of the functions of dispersion and discharge(疏泄), Iiver-energy(肝氣) is becoming degected, So overproduct and overflow of ascent and exhalation of liver-yang(肝陽) is becoming blood are ascending following energy. complete usage of Yin-blood(陰血) is responsible for some kinds of mass formed by blood stasis in the early stage of pathogenesis of liver disease syndrome of the energy system as the progession of disease extravasated blood is forming. the pathologic characteristics is appeared loss of control of the vital energy and blood(體用失調) at the liver disease. 4. Sthenia-syndrome of liver(肝實證) and liver-heat syndrome(肝熱證) is appered that overproducing and overflow of dispersion(疏泄太過) and discharge is responsible for overfunctioning of liver disease or some kinds of heat syndrome such as liver fire(肝火), Sthenia of liver-yang(肝陽上亢), the syndromes of sthenic liver heat(肝實熱) are appered. deficiency of the liver(肝虛證) and cold syndrome of liver(肝寒證) is classified pathologic characteristics of cold and heat, deficiency and excess that regression of sensory, motor, mental due to lack of dispersion and discharge(疏泄不及), or intruding of the cold miasma, are degected. 5. The liver is close relation of physiologic function and internal organ such as spleen, stomach, lung, heart, kidney, gall bladder by the meridian channels, because of property of wind Zang, rapid progession is classified by phthologic charateristics.

A Study of Air Freight Forecasting Using the ARIMA Model (ARIMA 모델을 이용한 항공운임예측에 관한 연구)

  • Suh, Sang-Sok;Park, Jong-Woo;Song, Gwangsuk;Cho, Seung-Gyun
    • Journal of Distribution Science
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    • v.12 no.2
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    • pp.59-71
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    • 2014
  • Purpose - In recent years, many firms have attempted various approaches to cope with the continual increase of aviation transportation. The previous research into freight charge forecasting models has focused on regression analyses using a few influence factors to calculate the future price. However, these approaches have limitations that make them difficult to apply into practice: They cannot respond promptly to small price changes and their predictive power is relatively low. Therefore, the current study proposes a freight charge-forecasting model using time series data instead a regression approach. The main purposes of this study can thus be summarized as follows. First, a proper model for freight charge using the autoregressive integrated moving average (ARIMA) model, which is mainly used for time series forecast, is presented. Second, a modified ARIMA model for freight charge prediction and the standard process of determining freight charge based on the model is presented. Third, a straightforward freight charge prediction model for practitioners to apply and utilize is presented. Research design, data, and methodology - To develop a new freight charge model, this study proposes the ARIMAC(p,q) model, which applies time difference constantly to address the correlation coefficient (autocorrelation function and partial autocorrelation function) problem as it appears in the ARIMA(p,q) model and materialize an error-adjusted ARIMAC(p,q). Cargo Account Settlement Systems (CASS) data from the International Air Transport Association (IATA) are used to predict the air freight charge. In the modeling, freight charge data for 72 months (from January 2006 to December 2011) are used for the training set, and a prediction interval of 23 months (from January 2012 to November 2013) is used for the validation set. The freight charge from November 2012 to November 2013 is predicted for three routes - Los Angeles, Miami, and Vienna - and the accuracy of the prediction interval is analyzed using mean absolute percentage error (MAPE). Results - The result of the proposed model shows better accuracy of prediction because the MAPE of the error-adjusted ARIMAC model is 10% and the MAPE of ARIMAC is 11.2% for the L.A. route. For the Miami route, the proposed model also shows slightly better accuracy in that the MAPE of the error-adjusted ARIMAC model is 3.5%, while that of ARIMAC is 3.7%. However, for the Vienna route, the accuracy of ARIMAC is better because the MAPE of ARIMAC is 14.5% and the MAPE of the error-adjusted ARIMAC model is 15.7%. Conclusions - The accuracy of the error-adjusted ARIMAC model appears better when a route's freight charge variance is large, and the accuracy of ARIMA is better when the freight charge variance is small or has a trend of ascent or descent. From the results, it can be concluded that the ARIMAC model, which uses moving averages, has less predictive power for small price changes, while the error-adjusted ARIMAC model, which uses error correction, has the advantage of being able to respond to price changes quickly.

Effects on the Thermal Changes of the Ankie Region Following Acupuncture on Wu-Hu (오호혈(五虎穴)이 족과부(足踝部) 체표온도변화(體表溫度變化)에 미치는 영향(影響))

  • Kim, Hyun-Jung;Byun, Jae-Young;Ahn, Soo-Gi;Lee, Geon-Mok;Park, Yong-Hyun;Kim, Kyung-Sik
    • Journal of Acupuncture Research
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    • v.18 no.1
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    • pp.40-49
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    • 2001
  • Background and Purpose : There have been many studys about the relationship between the meridians and the acupoints of the twelve meridians, but no study about the extra points. To verify Dong-Shi acupoints, a kind of extra points, we studied the effects on the thermal changes of the ankle region following acupuncture on the Wu-Hu. Object and Methods : This study was researched into clinical statistics for 45 healthy normal students. The object was divided into two groups. One was the control group and the other was the acupuncture group. The acupuncture group was divided into two groups ; ACU-up : The class of ascent thermal change among acupuncture group. ACU-dn : The class of descent thermal change among acupuncture group. First, in the control group, we took a picture at the ankle region(BL60, BL62, GB40, ST41) without any stimulation with the Digital Infrared Thermal Imaging System and did after 10 minutes respectively. Second, in the acupuncture group, we took a picture without any stimulation and then acupuncture on Wu-Hu and took a picture after 10 minutes in the same way respectively. Results : 1. In the control group, average thermal change of BL60, BL62, GB40 and ST41 was decreased(about $0.2^{\circ}C$) after 10 minutes. 2. The skin temperature of all measured parts was significantly lower in the ACU-dn group after 10 minutes. 3. There were no significant differences of thermal change between the control and the acupuncture group, between the control and ACU-up group after 10 minutes. Conclusion : This result indicated that the acupuncture on the Wu-Hu was effective at the ankle region. And so the more study is needed continuously.

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Deformation of Moho in the Southern Part of the Korean Peninsula (한반도 남부 모호면의 변형 구조)

  • Shin, Young-Hong;Park, Jong-Uk;Park, Pil-Ho
    • Journal of the Korean earth science society
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    • v.27 no.6
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    • pp.620-642
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    • 2006
  • The Moho structure and its deformation in the southern part of the Korean Peninsula were estimated using gravity and topography data. Gravity signals from the upper and lower crust were separated using a filter that was computed from isostacy and elastic thickness. The result of this study shows three characteristic features of the Moho deformation. First, the Moho folding structure is parallel to SKTL (the South Korean Tectonic Line), which indicates positive association with the collision of the Yeongnam and Gyeonggi Massifs and repeated compression afterwards. In contrast, noticeable deformation of the Moho was not observed along the Imjingang Belt, which is interpreted as another continental collisional belt in the Korean Peninsula. Second, the Moho beneath the Gyeongsang Basin has remarkably risen; this seems to be the result from both the collisional compression and buoyancy caused by magmatic underplating. Third, the Moho deformation is shallowest in the east of the Taebaek Mountains and deepens toward the west, consistent with the topographic characteristic of the Korean Peninsula of "high east and low west". It can be interpreted as the results of the opening of the East Sea and Ulleung Basin. A tectonic explanation for this could be the ascent of the mantle induced by continental rifting and horizontal extension at the early stage of the opening of the East Sea. The Moho deformation model computed in this study correlates well with the earthquake distribution and crustal movement measured by GPS. We suggest that the compression along the SKTL is still exerted, consequently, the Moho deformation is active, although it may be weak.

A development of the Vehicle-To-Vehicle communication system using the Dedicated Short Range Communication technology (근거리 무선통신 기술 기반 차량간통신 시스템 개발)

  • Rhee Eung-Ho
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.43 no.9 s.351
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    • pp.6-13
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    • 2006
  • In this paper, we studied vehicle to vehicle (VTV) communication system using DSRC of 5.8 GHz bands. Nowadays, in the road traffic system is going intelligent and advancing, communication between driving vehicle is very important technology for ITS. We can contrive smoothness and safety traffic flowing by exchanging information about velocity, location, braking and driving condition of nearby vehicles. Therefore, we developed and verified the system which required for the communication among vehicles using DSRC technology of 5.8 GHz band hasa 1 Mbps data rate in the high mobility condition. For this, we developed DSRC modem, data link layer and logic link layer to make it possible that communication between vehicles of perfectly operation, and developed application service program for VTV communication. We performed to communication test in the general road and ascent road. In case of the general mad, obtained VTV communication results are more than number of 17 with in 300 m LOS coverage, and total communication time are $2.34{\sim]18.7$ msec that considered maximum 8-transaction. We blow that obtained results can be used VTV communication or the in areas form the feasibility road test as a function or various conditions. In the future, this system is very useful of advanced safety vehicle (ASV) and super smart vehicle system (SSVS) and so on.