• Natural Product Sciences
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• 제9권4호
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• pp.264-269
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• 2003

The rhizome of Acorus calamus Linn. is commonly known as "Vacha" in indigenous systems of medicine. It is distributed in marshy tracts of Kashmir, Sirmaur (Himachal Pradesh), Manipur and the Naga hills. It is regularly cultivated in Koratagere Taluk in Karnataka and other parts of India. This study deals with the detailed pharmacognostical evaluation of the dried rhizomes of Acorus calamus collected from DehraDun (Uttaranchal), Lucknow (Uttar Pradesh). The commercial sample procured from Delhi market was also evaluated to observe the difference between collected and market samples. Dried rhizome is vertically compressed, pale yellow to dark brown and occasionally orangish brown in colour. Transverse section showed two distinct region with scattered, concentric vascular bundles surrounded by fibrous bundle sheath. Some vascular bundles just beneath the endodermis devoid of bundle sheath. Though the botanical and physico-chemical characters of all the samples were quite similar but some variations were observed in High Performance Thin Layer Chromatography (HPTLC) fingerprint profile, the essential oil content and total percentage of asarone which was found to be highest in Lucknow and lowest in Delhi market sample. These variations may be explained due to some edaphic factors or storage conditions. An attempt was also made to test antioxidant activity (in vitro) and it was found to be 88% at 0.2 g/ml concentration.

• 한국응용과학기술학회지
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• 제39권2호
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• pp.303-313
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• 2022

본 연구는 장흥지역에서 자생 및 재배한 총 3가지 식물을 수증기 증류 추출(편백, 석창포, 라벤더)을 통해 얻은 각 천연 에센셜 오일에 대한 GC-MSD 향기 성분 분석 후 추출된 오일들과 시판 오일(클라리세이지, 시나몬, 프랑킨센스)을 조합하여 세포독성, 항산화, 항염, 미백 효과를 확인하여 화장품 소재로서의 가능성을 확인하였다. 향기 성분 분석 결과 편백 오일의 주요 성분으로는 Sabinene, 석창포 오일은 Asarone, 라벤더 오일은 L-Linalool이 동정되었다. 6종 에센셜 조합 오일의 세포독성이 확인되지 않은 100 𝜇L/mL의 농도에서 NO 생성은 27.76% 억제, DPPH 라디칼 소거능은 99.69%, ABTS 라디칼 소거능은 94.66%, 티로시나아제 저해활성은 55.9%를 보여 기능성 화장품 원료로서 유용하게 활용할 수 있는 가능성을 제시하였다.

• 생약학회지
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• 제38권4호
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• pp.394-399
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• 2007

Eight compounds were isolated from the roots of Asarum sieboldii and their structures were identified as $({\pm})$-car-3-ene-2,5-dione (1), (-)-asarinin (2), (-)-sesamin (3), eucarvone (4), methyleugenol (5), ${\gamma}-asarone$ (6), pellitorine (7) and asarinol A (8) by analysis of spectral data. Among them, (-)-asarinin (2) showed the most potent inhibitory effect on melanogenesis, with inhibition rate of 66%.

• 대한암한의학회지
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• 제11권1호
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• pp.95-103
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• 2006

Objective: Gami-Samhwang-San, a herbal prescription for obesity treatment, is composed of seven crude herbs such as Ephedrae Herba, Scutellariae Radix, Acori Gramineri Rhizoma, Polygalae Radix, Typhae Pollen, Armeniacae Semen, Nelumbo Folium. This study was aimed to evaluate marker substances in n-butanol fraction (SH-21-B) from Gami-Samhwang-San by high performance liquid chromatography (HPLC). And we predicted inhibition activity of major compounds of Gami-Samhwang-San using Quantitative Structure Activity Relationships (QSAR) Methods: The separation was performed on a YMC J,sphere-H80 CI8(250${\times}$4.6 mm I.D) column by gradient elution with $H_3PO_4$ buffers in acetonitrile as the moblie phase at a flow-rate of 1.0ml/min. Results: HPLC was employed to determine the quantities and the qualities of several marker substances such as ephedrine, pseudoephedirne, baicalin, ${\beta}-asarone$, tenuifoliside, naringenin, amygdalin and hyperoside in the SH-21-B. Conclusion: We suggest this results could be a useful evidence for quality control of SH-21-B.

• 생약학회지
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• 제51권4호
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• pp.238-243
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• 2020

Perilla frutescens Britton var. acuta Kudo, an annual plant primarily cultivated in China, Japan, and Korea, has been used as a traditional medicine to treat inflammatory diseases, depression, and many anxiety-related disorders. Previously, we reported the inhibitory effects of n-hexane layer of P. frutescens var. acuta extract against beta-amyloid (Aβ) aggregation, and the isolation of asarone derivatives as active constituents from n-hexane layer. In this study, dichloromethane layer of P. frutescens var. acuta was applied to bioassay-guided isolation methods accompanied with Thioflavin T (Th T) fluorescence assay to investigate the inhibitory effect on Aβ aggregation and disaggregation. As the results, three triterpenoids including ursolic acid (1), tormentic acid (2) and corosolic acid (3) were isolated. All compounds reduced Aβ aggregation and increased disaggregation of preformed Aβ aggregates in a dose-dependent manner. However, the inhibitory effect of three compounds on Aβ aggregation was not correlated with antioxidant activity, which was measured by DPPH assay. Taken together, these results suggest that the triterpenoid derivatives from P. frutescens have the potential to be developed as good therapeutics or preventatives for AD.

• 한국식품영양학회지
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• 제33권2호
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• pp.215-221
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• 2020

The purpose of this study was to investigate the protective effect on oxidative stress induced PC12 cells, and volatile flavor composition of essential oils derived from medicinal plant seeds- Gossypium hirsutum L. (G. hirsutum), Coix lachryma-jobi (C. lachryma-jobi) and Oenothera biennis (O. biennis). The essential oils were obtained by the solvent (hexane) extraction method from the seeds. The essential oils of the seeds were analyzed by the solid-phase micro-extraction gas chromatography mass spectrometry (SPME-GC/MS). The major compounds of G. hirsutum, C. lachryma-jobi and O. biennis were cyclonexanol (16.65%), β-asarone (14.29%) and ylangene (50.01%). The DPPH radical scavenging activity (IC₅₀) was the highest value of 8.52 mg/mL in the O. biennis. Additionally, IC₅₀ values of G. hirsutum and C. lachryma-jobi were 26.76 mg/mL and 36.81 mg/mL. For the oxidative stress on PC12 cells, we treated with hydrogen peroxide (H₂O₂). The pretreatment of oxidative stress induced PC12 cells with all the essential oils preserved or increased their cell viability and G. hirsutum and O. biennis attenuated the ROS generation (by 68.75% and 56.25% vs. H₂O₂ control). The results of this study suggest that the essential oils derived from medicinal plant seeds could be used as valuable back data as a natural essential oil material to prevent neurodegenerative diseases by protecting neuro-cells.

• 한국식품위생안전성학회지
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• 제35권4호
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• pp.326-333
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• 2020

본 연구는 불법적으로 식품에 사용될 수 있는 부정물질 11종에 대한 안전관리 강화를 위해 정량 및 정성 분석이 가능한 HPLC-DAD와 LC-MS/MS를 검증하기 위해 수행되었다. 확립된 시험법은 AOAC 가이드라인에 따라 직선성, 정밀성, 정량한계 및 회수율 등을 통해 유효성을 확인하였다. 본 실험에서 정량한계를 포함하여 검량선을 작성하였고, 모두 0.99 이상의 직선성을 확인하였다. 또한 정확성은 LC (90.0-106%), LC-MS/MS (83.0-114%) 이고, 정밀도는10% 이하로 재현성이 우수하였다. 확립된 시험법은 식품 중 부정물질 안전관리 및 모니터링에 활용될 것으로 사료된다.

• 한국산림과학회지
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• 제102권3호
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• pp.309-314
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• 2013

산림공기 중 약리효과를 주는 것으로 알려진 모노테르펜류의 분포양태 파악을 위해 고흥 팔영산, 보성 제암산, 장흥 우드랜드 숲속 공기와 그 주변에 우점하고 있는 나무의 정유에서 ${\alpha}$-pinene, camphene, ${\beta}$-pinene, 2-carene, myrcene, ${\alpha}$-phellandrene, ${\alpha}$-terpinene, d-limonene, eucaliptol, cymene, terpinolene, linalool, camphor, menthol, borneol, thymol, asarone, coumarine 농도분포를 비교하였다. 숲속 공기는 3 계절(봄, 여름, 가을), 정유는 봄, 여름기간 수행하였다. 공기 중에는 4 시간 평균농도 기준으로 ${\alpha}$-pinene, ${\beta}$-pinene과 2-carene이 각각 높은 농도로 나타났다. 측정된 3계절 평균 농도는 소나무가 우점하고 있는 보성 제암산에서 2,597 pptv로 가장 높았다. 계절별로는 고흥 팔영산과 보성 제암산은 봄, 장흥 우드랜드는 가을에 최고치를 보였다. 정유 중 테르펜은 편백나무가 우점하고 있는 고흥 팔영산이 가장 높았고, 장흥 우드랜드 >보성 제암산 순으로 숲속 공기와는 다른 분포형태를 보였다. 주요 성분도 달라 고흥 팔영산은 sabinene, myrcene, limonene이 높은 반면 장흥 우드랜드는 ${\alpha}$-terpinene, linalool, camphor가 보성 제암산은 cymene, camphor, borneol이 높게 나타났다. 정유 중 함량은 10~20령의 비교적 어린 편백나무가 높았고, 특히 봄철에 매우 높게 조사되어 정유를 활용하는 산림산업에 수종 및 채취시기 등을 시사해준다.