• Title/Summary/Keyword: As-extruded

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Parthenogenetic Development of Rabbit Oocytes Activated by Ionomycin Plus 6-Dimethylaminopurine Treatments (Ionomycin과 6-Dimethylaminopurine(6-DMAP)처리로 활성화된 토끼 난자의 단위발생)

  • 윤희준;이효종;최상용;박충생
    • Korean Journal of Animal Reproduction
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    • v.22 no.3
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    • pp.221-227
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    • 1998
  • This study was carried out to determine the effects of ionomycin and 6-dimethylaminopurine (6-DMAP) treatments on parthenogenesis of rabbit oocytes. The oocytes were randomly assigned to the activation treatments with either ionomycin plus 6-DMAP or electric stimulation. The oocytes were colected from the oviducts of superovulated rabbits at 13~14 hours and 19~20 hours post hCG injection and were activated with 5$\mu$M ionomycin for 5 min and 2 hours incubation in 2mM 6-DMAP. The other oocytes were stimulated by three pulses of 3.6kV/cm for 60 $\mu$sec each 30 min apart, starting 19 hours post in hCG in 0.28M mannitol solution with 100$\mu$M Ca2+ and Mg2+. The results obtained were summarized as follows: 1. Following treatment of the oocytes with ionomycin plus 6-DMAP, the cleavage rate and in vitro developmental rate to blastocyst were significantly(P<0.01) higher in the oocytes collected bet ween 19~20 hours than between 13~14 hours after hCG injection. 2. When the oocytes were treated with ionomycin plus 6-DMAP, 85(98.8%) of 86 treated oocytes extruded the second polar bodies, with the entire chromatin complements outside ooplasm. However when the oocytes were restored during subsequent incubation in the drug-free medium, the cytoplasts regain their full capacity for parthenogenetic activation and nuclear remodelling. 3. The cleavage rate and the in vitro developmental rate to blastocyst were not significantly different in the oocytes activated by ionomycin plus 6-DMAP treatment(91.2 and 45.6%) or electrical stimulation(89.6 and 34.3%).

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Preparation and Hypoglycemic Effect of Reconstituted Grain Added with Selected Medicinal Herb Extract (한방 생약재 추출물을 첨가한 재성형 당뇨쌀의 제조 및 혈당강하 효과)

  • Lee, Young-Tack
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.527-531
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    • 2003
  • Hot-water extract from selected medicinal herbs of diabetes prescription was mixed with brown rice and pearled barley flours and extruded to prepare a reconstituted grain for diabetes millitus patients. Even though the cooked reconstituted grain containing medicinal herb extract was somewhat different from cooked ordinary rice in textural properties measured by texture analyzer, it was estimated to be organoleptically acceptable in sensory parameters. The reconstituted grain added with medicinal herb extract reduced the rate of dialysis of glucose evaluated by in vitro dialysis experiment. The effects of reconstituted grain diet on blood glucose levels in diabetes patients were studied during 3-week period. Compared to normal diet, the reconstituted grain diet for 2 weeks significantly decreased the fasting and 2-hr postprandial blood glucose levels in diabetes patients by 14% and 10%, respectively. These results suggested that the reconstituted grain could be used as an effective therapeutic diet for the control of diabetes mellitus.

Effects of Processing Parameters of Twin Screw Extruder and Dry Methods on the Resistant Starch Formation from Normal Maize Starch (압출성형공정과 건조조건이 옥수수전분의 저항전분 수율에 미치는 영향)

  • Shin, Mal-Shick;Mun, Sae-Hun;Bae, Chun-Ho
    • Korean Journal of Human Ecology
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    • v.5 no.1
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    • pp.62-70
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    • 2002
  • The objective of this study was to evaluate the effects of processing parameters of co-rotating twin screw extruder and dry methods on the level of resistant starch (RS) and the properties of extrudate prepared from normal maize starch. The processing parameters were used 90, 110, 130$^{\circ}C$ in temperature, 25.0~30.0% in moisture content, 150, 200, 250 rpm in screw speed and hot and cool air drying and drying after refrigerating in drying methods. The barrel temperature and drying methods had affected the level of resistant starch of extrudate. RS levels of extrudates were ranged from 2.4 to 15.5% by AOAC method. The extrudates, extruded at 110$^{\circ}C$ and then stored at 4$^{\circ}C$, showed the highest level of RS level (15.5%). Water absorption index increased with increasing moisture content and peak temperatures and viscosities of extudates decreased compare to that of raw starch from 94$^{\circ}C$ to 50~65$^{\circ}C$ and from 220 to 46~98 RVU, respectively. Extudates treated in 90$^{\circ}C$ and 110$^{\circ}C$ showed strong peak at $2{\theta}=6.7{\sim}17.0^{\circ}$ by X-ray diffractometry and had ~150$^{\circ}C$ endotherm like as that of RS3 starch by differential scanning calorimetry. In case of 130$^{\circ}C$, extrudates showed strong peak at $2{\theta}=20.0^{\circ}$ and had 106$^{\circ}C$ endotherm.

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Useful Corrosion - Potential of Magnesium Alloys as Implants

  • Kaya, A. Arslan;Kaya, R. Alper;Witte, Frank;Duygulu, Ozgur
    • Corrosion Science and Technology
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    • v.7 no.3
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    • pp.162-167
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    • 2008
  • Degradable implants have been in use for bone surgery for decades. However, degradable metal implants are one of the new research areas of biomaterials science. Magnesium has good biocompatibility due to its low toxicity, and it is a corroding, i.e. dissolvable, metal. Furthermore, magnesium is needed in human body, and naturally found in bone tissue. There have been some published reports also asserting the potential bone cell activation or bone healing effect of high magnesium ion concentrations. The classic method for achieving intertransverse process fusion involves autogenous iliac crest bone graft. Several investigations have been performed to enhance this type of autograft fusion. However, there is no research which has been undertaken to investigate the efficiency of pure magnesium particles in posterolateral spinal fusion. In this study, corrosion behavior of magnesium metal at the bone interface, the possibility of new bone cell formation and the degree of effectiveness in producing intertransverse process lumbar fusion in a sheep model have been investigated. Cortical bone screws were machined from magnesium alloy AZ31 extruded rod and implanted to hip-bones of sheep via surgery. Three months after surgery, the bone segments carrying these screws were removed from the sacrificed animals. Samples were sectioned to reveal Mg/bone interfaces and investigated using optical microscope, SEM-EDS and radiography. Optical and SEM images showed that there was a significant amount of corrosion on the magnesium screw. The elemental mapping results indicate, due to the presence of calcium and phosphorus elements, that there exists new bone formation at the interface. Furthermore, sixteen sheep were subjected to intertransverse process spinal fusions with pedicle screw fixation at various locations along their spines. Each animal was treated with 5cc autograft bone at one fusion level and 1cc magnesium+5cc autograft bone at the other. Six months after surgery, bone formation was evaluated by gross inspection and palpation, and radiological, histological, scanning electron microscopic and x-ray diffraction analyses. It may be stated that the potential for using useful corrosion of magnesium alloys in medical applications is expected to be significant.

Effect of Ca Addtion on Microstructure and Mechanical Properties of Mg-11Li-3Zn-1Sn-0.4Mn Based Alloys (Mg-11Li-3Zn-1Sn-0.4Mn 마그네슘 합금의 Ca 첨가에 따른 미세조직 및 기계적 특성평가)

  • Kim, Jung-Han;Kim, Yong-Ho;Yoo, Hyo-Sang;Son, Hyeon-Taek;Lee, Seong-Hee
    • Korean Journal of Materials Research
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    • v.25 no.6
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    • pp.269-273
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    • 2015
  • The effect of adding Ca on the microstructural and mechanical properties of as-cast Mg-11Li-3Zn-1Sn(wt%) alloys were investigated. Mg-11Li-3Zn-1Sn-0.4Mn with different Ca additions (0.4, 0.8, 1.2 wt%) were cast under an $SF_6$ and $Co_2$ atmosphere at $720^{\circ}C$. The cast billets were homogenized at $400^{\circ}C$ for 12h and extruded at $200^{\circ}C$. The microstructural and mechanical properties were analyzed by OM, XRD, SEM, and tensile tests. The addition of Ca to the Mg-11Li-3Zn-1Sn-0.4Mn alloy resulted in the formation of $Ca_2Mg_6Zn_3$, MgSnCa intermetallic compound. By increasing Ca addition, the volume fraction and size of $Ca_2Mg_6Zn_3$ with needle shape were increased. This $Ca_2Mg_6Zn_3$ intermetallic compound was elongated to the extrusion direction and refined to fine particles due to severe deformation during hot extrusion. The elongation of the 0.8 wt% Ca containing alloy improved remarkably without reduction strength due to the formation of fine grain and $Ca_2Mg_6Zn_3$ intermetallic compounds by Ca addition. It is probable that fine and homogeneous $Ca_2Mg_6Zn_3$ intermetallic compounds played a significant role in the increase of mechanical properties.

Hot Metal Extru-Bending Process for Curved Aluminum Tube Products with Circular or Rectangular Sections (원형 또는 사각 단면을 가지는 알루미늄 곡관 튜브제품의 열간금속압출굽힘가공)

  • Park D. Y;Jin I. T
    • Transactions of Materials Processing
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    • v.13 no.8
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    • pp.663-670
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    • 2004
  • The bending phenomenon has been known to be occurred by the difference of velocity at the die exit. The difference of velocity at the die exit section can be obtained by the different velocity of billets through the multi-hole container. The difference of velocity at the die exit can be controlled by the two variables, the one of them is the different velocity of extrusion punch through the multi-hole container, the other is the difference of hole diameter of muliti-hole container. In this paper the difference of hole diameter is applied. So it can bend during extruding products because of the different amount of two billets when billets would be bonded in the porthole dies cavity. And the bending curvature can be controlled by the size of holes. The experiments with aluminum material for the curved tube product had been done for circular or rectangular curved tube section. The results of the experiments show that the curved tube product can be formed by the extru-bending process without the defects such as distortion of section and thickness change of wall of tube and folding and wrinkling. The curvature of product can be controlled by shape of cross section and the difference of billet diameters. And it is known that the bonding and extruding and bending process can be done simultaneously in the die cavity by the experiments that rectangular hollow curved tubes could be extruded by porthole dies with four different size billets made of aluminum material. And it shows that bending phenomenon can happen during extruding with for different billets from the analysis by DEFORM-3D.

Effects of Extrusion Frequency on the Quality Characteristics of Ddukgukdduk (압출성형 횟수가 떡국떡의 품질 특성에 미치는 영향)

  • Bae, Jeong-Sook;Yoo, Chang-Hee;Lee, Kyung-Eun
    • Korean journal of food and cookery science
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    • v.32 no.4
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    • pp.449-457
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    • 2016
  • Purpose: The study was conducted to analyze the change in quality characteristics of Ddukguk Garaedduk and Ddukgukdduk by the frequency of extruding rice dough. Methods: Ddukguk Garaedduk samples were made by extruding and shaping rice flour dough 2, 3, and 4 times; while Ddukgukdduk samples were made by drying the Ddukguk Garaedduk at room temperature for 48 hours and slicing them in slices of 0.3 cm thickness. Results: Significant differences were not found in water content by the extrusion frequency for Ddukguk Garaedduk and Ddukgukdduk. The adhesiveness and springiness of Ddukguk Garaedduk with a 4 times extrusion were significantly higher than those of Ddukguk Garaedduk with a 2 times extrusion. Ddukgukdduk with a 4 times extrusion generated finer cells and a more even distribution of cells than the other samples, as observed by scanning electron microscopy. Upon boiling, significantly more water was absorbed in Ddukgukdduk of a 2 times extrusion than in that of a 4 times extrusion. Hardness and adhesiveness scores of the cooked Ddukgukdduk were the highest, and springiness was the lowest in the sample with the 4 times extrusion. Conclusion: The extrusion of rice dough makes the structure of Ddukguk Garaedduk finer and more even, which results in the increased adhesiveness of Ddukguk Garaedduk. When cooking Ddukguk, Ddukgukdduk that has been extruded more absorbs less water and dissolves less solids, which maintains the chewiness and structure of cooked Ddukgukdduk.

Strength and compressibility characteristics of peat stabilized with sand columns

  • Jorat, M. Ehsan;Kreiter, Stefan;Morz, Tobias;Moon, Vicki;de Lange, Willem
    • Geomechanics and Engineering
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    • v.5 no.6
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    • pp.575-594
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    • 2013
  • Organic soils exhibit problematic properties such as high compressibility and low shear strength; these properties may cause differential settlement or failure in structures built on such soils. Organic soil removal or stabilization are the most important methods to overcome geotechnical problems related to peat soils' engineering characteristics. This paper presents soil mechanical intervention for stabilization of peat with sand columns and focuses on a comparison between the mechanical characteristics of undisturbed peat and peat stabilized with 20%, 30% and 40% of sand on the laboratory scale. Cylindrical columns were extruded in different diameters through a nearly undisturbed peat sample in the laboratory and filled with sand. By adding sand columns to peat, higher permeability, higher shear strength and a faster consolidation was achieved. The sample with 70% peat and 30% sand displayed the most reliable compressibility properties. This can be attributed to proper drainage provided by sand columns for peat in this specific percentage. It was observed that the granular texture of sand also increased the friction angle of peat. The addition of 30% sand led to the highest shear strength among all mixtures considered. The peat samples with 40% sand were sampled with two and three sand columns and tested in direct shear and consolidation tests to evaluate the influence of the number and geometry of sand columns. Samples with three sand columns showed higher compressibility and shear strength. Following the results of this laboratory study it appears that the introduction of sand columns could be suitable for geotechnical peat stabilization in the field scale.

Optimization of Extrusion Process Conditions to Increase the Corn Fiber Gum and Soluble Arabinoxylan Yield from Corn Fiber (옥수수 섬유질로부터 검과 수용성 아라비노자일란의 수율향상을 위한 압출성형 조건의 최적화)

  • Jeon, Sujung;Ryu, Gihyung
    • Korean Journal of Food Science and Technology
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    • v.47 no.2
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    • pp.149-157
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    • 2015
  • The effects of feed moisture content (25, 35, and 45%), screw speed (230, 250, and 270 rpm), and barrel temperature (130, 140, and $150^{\circ}C$) on the product yield and soluble arabinoxylan (SAX) content from destarched corn fiber (DCF), and its optimization were investigated. The yield and SAX content of corn fiber gum (CFG) from the extruded destarched corn fiber (EDCF) were higher than those of DCF. Statistical analyses revealed that the feed moisture content and barrel temperature had a significant effect on the CFG yield and total SAX content. The optimum extrusion pretreatment conditions were as follows: feed moisture content, 30%; screw speed, 260 rpm; barrel temperature, $133^{\circ}C$. This study showed that the response surface methodology was suitable for the optimization of the extrusion conditions used to maximize the CFG yield and total SAX content from EDCF.

A Study on the Strength of the Helmets with a Lobe in the Summit (정상 돌출부를 갖는 안전모의 강도 안전성에 관한 연구)

  • Kim, Chung Kyun
    • Journal of the Korean Institute of Gas
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    • v.17 no.5
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    • pp.37-41
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    • 2013
  • This paper presents the strength safety of stress and deformation behaviors using the finite element method as a function of the thickness of the protective helmets with and without an extruder on the top of the shell structure. The helmet that would provide head and neck protections without causing discomfort to the user when it was worn for long periods of time should be manufactured for increasing the safety and impact energy absorption. The stress analyzed results show that when the impulsive force of 4,540N is applied on the top surface of a helmet, the safe thickness is 3.7mm for the conventional helmet and 3.2mm for the modified new helmet. Based on the deformation analysis, the FEM results recommend that the safe thickness is 3.2mm for the conventional helmet and 2.0mm for the modified new helmet. Thus, it may be more safe design of the helmet, which has an extruded structure on the summit surface of the helmet.