• Journal of Microbiology and Biotechnology
• /
• v.26 no.7
• /
• pp.1290-1302
• /
• 2016

Microalgae are being researched for their potential as attractive biofuel feedstock, particularly for their lipid production. For maximizing biofuel production, it is necessary to explore the effects of environmental factors on algal lipid-producing potential. In this study, the effects of nitrogen (N) sources (NO₂-N, NO₃-N, urea-N, NH₄-N, and N-deficiency) and carbon-to-nitrogen ratios (C/N= 0, 1.0, 3.0, and 5.0) on algal lipid-producing potential of Chlorella sp. HQ were investigated. The results showed that for Chlorella growth and lipid accumulation potential, NO₂-N was the best amongst the nitrogen sources, and NO₃-N and urea-N also contributed to algal growth and lipid accumulation potential, but NH₄-N and N-deficiency instead caused inhibitory effects. Moreover, the results indicated that algal lipid-producing potential was related to C/N ratios. With NO₂-N treatment and carbon addition (C/N = 1.0, 3.0, and 5.0), total lipid yield was enhanced by 12.96-20.37%, but triacylglycerol (TAG) yields decreased by 25.52-94.31%. As for NO₃-N treatment, carbon addition led to a 17.82-57.43%/25.86-82.67% reduction of total lipid/TAG yields. When NH₄-N was used as the nitrogen source, total lipid/TAG yields were increased by 46.67-113.33%/28.99-74.76% with carbon addition. The total lipid/TAG yields of urea-N treatment varied with C/N ratios. Overall, the highest TAG yield (TAG yield: 38.75 ± 5.21 mg/l; TAG content: 44.16 ± 4.35%) was achieved under NO₂-N treatment without carbon addition (C/N = 0), the condition that had merit for biofuel production.

• The Plant Pathology Journal
• /
• v.28 no.1
• /
• pp.40-48
• /
• 2012

$Potato$ $virus$ $X$ (PVX) replication is precisely regulated by regulatory viral sequences and by viral and/or host proteins. In a previous study, we identified a 54-kDa cellular tobacco protein that bound to a region within the first 46 nucleotides (nt) of the 5' non-translated region (NTR) of the viral genome. Optimal binding was dependent upon the presence of an ACCA sequence at nt 10-13. To identify host factors that bind to 5' NTR elements including AC-rich sequences as well as stemloop 1 (SL1), we used northwestern blotting and matrixassisted laser desorption/ionization time-of-flight mass spectrometry for peptide mass fingerprinting. We screened several host factors that might affect PVX replication and selected a candidate protein, $Nicotiana$ $tabacum$ WRKY transcription factor 1 (NtWRKY1). We used a $Tobacco$ $rattle$ $virus$ (TRV)-based virus-induced gene silencing (VIGS) system to investigate the role of NtWRKY1 in PVX replication. Silencing of $WRKY1$ in $Nicotiana$ $benthamiana$ caused lethal apical necrosis and allowed an increase in PVX RNA accumulation. This result could reflect the balancing of PVX accumulation in a systemic $N.$ $benthamiana$ host to maintain PVX survival and still produce a suitable appearance of mosaic and mottle symptoms. Our results suggest that PVX may recruit the WRKY transcription factor, which binds to the 5' NTR of viral genomic RNA and acts as a key regulator of viral infection.

• 전자공학회논문지 IE
• /
• v.48 no.2
• /
• pp.6-11
• /
• 2011

This paper describes the design of new method of propagation delay measurement in micro and nanostructures during characterization of ASIC standard library cell. Providing more accuracy timing information about library cell (NOR, AND, XOR, etc.) to the design team we can improve a quality of timing analysis inside of ASIC design flow process. Also, this information could be very useful for semiconductor foundry team to make correction in technology process. By comparison of the propagation delay in the CMOS element and result of analog SPICE simulation, we can make assumptions about accuracy and quality of the transistor's parameters. Physical implementation of phase error accumulation method(PHEAM) can be easy integrated at the same chip as close as possible to the device under test(DUT). It was implemented as digital IP core for semiconductor manufacturing process($0.11{\mu}m$, GL130SB). Specialized method helps to observe the propagation time delay in one element of the standard-cell library with up-to picoseconds accuracy and less. Thus, the special useful solutions for VLSI schematic-to-parameters extraction (STPE), basic cell layout verification, design simulation and verification are announced.

• Journal of the Korean Institute of Telematics and Electronics
• /
• v.27 no.11
• /
• pp.49-54
• /
• 1990

In this paper, a simple model is presented for the gate-voltage dependence of the parasitic resistance in MOSFETs with the lightly-doped drain (LDD) structure. At the LDD region located under the gate electrode, an accumulation layer is formed due to the gate voltage. The parasitic resistance of the source side LDD in the channel is treated as a parallel combination of the resistance of the accumulation layer and that of the bulk LDD, which is approximated as a spreading resistance from the end of the channel inversion layer to the ${n^+}$/LDD junction boundary. Also the effects of doping gradients at the junction are discussed. As result of the model, the LDD resistance decreases with increasing the gate voltage at the linear regime, and increase quasi-linearly with the gate voltage at the saturation regime, considering th velocity saturation both in the channel and in the LDD region. The results are in good agreement with experimental data reported by others.

• Journal of Korean Medicine for Obesity Research
• /
• v.20 no.1
• /
• pp.1-9
• /
• 2020

Objectives: Artemisinin and its derivatives extracted from Artemisia annua, a Chinese herbal medicine, have variable biological effects due to structural differences. Up to date, the anti-obesity effect of dihydroartemisinin (DHA), a derivative of artemisinin, is unknown. The purpose of this study was to investigate the anti-adipogenic and lipolytic effects of DHA on 3T3-L1 preadipocytes. Methods: Oil Red O staining and AdipoRed assay were used to measure lipid accumulation and triglyceride (TG) content in 3T3-L1 cells, respectively. Cell count analysis was used to determine the cytotoxicity of 3T3-L1 cells. Western blot and real-time reverse transcription polymerase chain reaction analyses were used to analyze the expression of protein and mRNA in 3T3-L1 cells, respectively. Results: DHA at 5 μM markedly inhibited lipid accumulation and reduced TG content in differentiating 3T3-L1 cells with no cytotoxicity. Furthermore, DHA at 5 μM inhibited the expression of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A as well as the phosphorylation of signal transducer and activator of transcription-3 (STAT-3) in differentiating 3T3-L1 cells. Moreover, while DHA at 5 μM had no effect on the mRNA expression of adiponectin, it strongly suppressed that of leptin in differentiating 3T3-L1 cells. However, DHA at 5 μM had no lipolytic effect on differentiated 3T3-L1 cells, as assessed by no enhancement of glycerol release. Conclusions: These results demonstrate that DHA at 5 μM has a strong anti-adipogenic effect on differentiating 3T3-L1 cells through the reduced expression and phosphorylation of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3.

• Nutrition Research and Practice
• /
• v.15 no.5
• /
• pp.555-567
• /
• 2021

BACKGROUND/OBJECTIVES: Abeliophyllum distichum is a plant endemic to Korea, containing several beneficial natural compounds. This study investigated the effect of A. distichum leaf extract (ALE) on adipocyte differentiation. MATERIALS/METHODS: The cytotoxic effect of ALE was analyzed using cell viability assay. 3T3-L1 preadipocytes were differentiated using induction media in the presence or absence of ALE. Lipid accumulation was confirmed using Oil Red O staining. The mRNA expression of adipogenic markers was measured using RT-PCR, and the protein expressions of mitogen-activated protein kinase (MAPK) and peroxisome proliferator-activated receptor gamma (PPAR𝛾) were measured using western blot. Cell proliferation was measured by calculating the incorporation of Bromodeoxyuridine (BrdU) into DNA. RESULTS: ALE reduced lipid accumulation in differentiated adipocytes, as indicated by Oil Red O staining and triglyceride assays. Treatment with ALE decreased the gene expression of adipogenic markers such as Ppar𝛾, CCAAT/enhancer binding protein alpha (C/ebp𝛼), lipoprotein lipase, adipocyte protein-2, acetyl-CoA carboxylase, and fatty acid synthase. Also, the protein expression of PPAR𝛄 was reduced by ALE. Treating the cells with ALE at different time points revealed that the inhibitory effect of ALE on adipogenesis is higher in the early period treatment than in the terminal period. Furthermore, ALE inhibited adipocyte differentiation by reducing the early phase of adipogenesis and mitotic clonal expansion. This was indicated by the lower number of cells in the Synthesis phase of the cell cycle (labeled using BrdU assay) and a decrease in the expression of early adipogenic transcription factors such as C/ebp𝛽 and C/ebp𝛿. ALE suppressed the phosphorylation of MAPK, confirming that the effect of ALE was through the suppression of early phase of adipogenesis. CONCLUSIONS: Altogether, the results of the present study revealed that ALE inhibits lipid accumulation and may be a potential agent for managing obesity.

• Korean Journal of Medicinal Crop Science
• /
• v.28 no.6
• /
• pp.463-470
• /
• 2020

Background: Evaluation of transpiration is required for agricultural and environmental management applications, as crop yields and plant growth are primarily water limited. This study aimed to determine the transpiration and carbon accumulation of Cnidium officinale. Methods and Results: The transpiration of C. officinale was evaluated using weighing lysimeter. The relationship between transpiration and factors such as solar radiation, air temperature, and leaf area was assessed. Transpiration increased as the leaf area increased with the growth stage. Furthermore, daily transpiration per unit leaf area was 0.69 ± 0.16 g·cm-2·day-1 and there were no significant differences in daily transpiration during the cultivation period. The maximum transpiration was 620.6 g m-2·h-1 and diurnal changes in transpiration were highly correlated with solar radiation although the maximum transpiration was observed at the air temperatures of 20℃ - 26℃. The ratio of carbon accumulation to transpiration was 0.12%. Conclusions: Our results indicated that the transpiration of C. officinale is primarily regulated by solar radiation energy on clear days and that 97% of the water is discharged through transpiration for heat dissipation. Therefore, weighing lysimeters can measure transpiration accurately and may be useful in interpreting plant growth.

• The Journal of Information Systems
• /
• v.31 no.4
• /
• pp.145-160
• /
• 2022

Purpose The purpose of this study is to develop a virtual try-on deep learning model that can efficiently learn front and back clothes images. It is expected that the application of virtual try-on clothing service in the fashion and textile industry field will be vitalization. Design/methodology/approach The data used in this study used 232,355 clothes and product images. The image data input to the model is divided into 5 categories: original clothing image and wearer image, clothing segmentation, wearer's body Densepose heatmap, wearer's clothing-agnosting. We advanced the HR-VITON model in the way of Mixed-Precison, Gradient Accumulation, and sharing model weights. Findings As a result of this study, we demonstrated that the weight-shared MP-GA HR-VITON model can efficiently learn front and back fashion images. As a result, this proposed model quantitatively improves the quality of the generated image compared to the existing technique, and natural fitting is possible in both front and back images. SSIM was 0.8385 and 0.9204 in CP-VTON and the proposed model, LPIPS 0.2133 and 0.0642, FID 74.5421 and 11.8463, and KID 0.064 and 0.006. Using the deep learning model of this study, it is possible to naturally fit one color clothes, but when there are complex pictures and logos as shown in <Figure 6>, an unnatural pattern occurred in the generated image. If it is advanced based on the transformer, this problem may also be improved.

• The Korea Journal of Herbology
• /
• v.39 no.2
• /
• pp.27-33
• /
• 2024

Objective : Obesity is a disease caused by energy imbalance and increases the risk of complications such as cardiovascular disease and cancer. Additionally, a recent study reported that excessive production of ROS stimulates the expression of transcription factors related to adipogenesis, such as PPAR-𝛾, C/EBP-𝛼 and 𝛽, in 3T3-L1 cells. In this study, Paeonia anomala L. (PAL) was selected as a candidate for improving and preventing obesity from Mongolian medical literature, and its effect on fat metabolism was observed in vitro. Methods : The activity of PAL extract against lipid accumulation was measured in 3T3-L1 cells through Oil red O staining. Additionally, the expression of lipid metabolism-related proteins was measured by western blot. Results : Intracellular lipid accumulation was significantly lower in the treatment group than in the control group. In addition, pAMPK/AMPK protein expression in the treatment group tended to increase in a concentration-dependent manner, and PPAR-𝛾 protein expression in the treatment group showed a significant decrease in a concentration-dependent manner compared to the control group. Conclusion : These results suggest that PAL extract may have an anti-obesity effect, and it is believed that it can be used to develop functional materials for the treatment of obesity.

• Journal of Korean Society of Water and Wastewater
• /
• v.19 no.1
• /
• pp.38-46
• /
• 2005

In this research, we tried to combine the coagulation/sedimentation process as pre-treatment with UF membrane filtration to reduce the membrane fouling and to improve the permeate water quality. We used the Jet Mixed Separator (JMS) as coagulation/sedimentation process. We observed that the HPC and E.Coli can't be removed through the direct UF memebrane filtation of surface water. The removal efficiency of dissolved organic substances, indicated by E260 and DOC, was 40% and 15%, respectively. However, the removal efficiency of it increased two time as a result of combination of JMS process as coagulation/sedimentation pre-treatment. This was resulted from the formation of high molecular humic micro-floc through JMS process. The accumulation amount of irreversible cake layer which was not removed by backwashing was less than direct UF membrane filtration of surface water. Moreover, the loading rate of fouling induced substances, such as humic substances and suspended substances, on membrane surface decreased drastically through JMS process. As a result, the accumulation amount of irreversible cake on membrane surface was decreased.