• Title/Summary/Keyword: Artificial surface defects

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Effect of Calvarial Cell Inoculated Onto the Biodegradable Barrier Membrane on the Bone Regeneration (흡수성 차폐막에 접목된 두개관골세포의 골조직 재생에 미치는 영향)

  • Yu, Bu-Young;Lee, Man-Sup;Kwon, Young-Hyuk;Park, Joon-Bong;Herr, Yeek
    • Journal of Periodontal and Implant Science
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    • v.29 no.3
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    • pp.483-509
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    • 1999
  • Biodegradable barrier membrane has been demonstrated to have guided bone regeneration capacity on the animal study. The purpose of this study is to evaluate the effects of cultured calvarial cell inoculated on the biodegradable barrier membrane for the regeneration of the artificial bone defect. In this experiment 35 Sprague-Dawley male rats(mean BW 150gm) were used. 30 rats were divided into 3 groups. In group I, defects were covered periosteum without membrane. In group II, defects were repaired using biodegradable barrier membrane. In group III, the defects were repaired using biodegradable barrier membrane seeded with cultured calvarial cell. Every surgical procedure were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium(30mg/Kg). After anesthesia, 5 rats were sacrificed by decapitation to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. The membrane were inserted on the artificial bone defect after 3 days of culture. A single 3-mm diameter full-thickness artificial calvarial defect was made in each animal by using with bone trephine drill. After the every surgical intervention of animal, all of the animals were sacrificed at 1, 2, 3 weeks after surgery by using of perfusion technique. For obtaining histological section, tissues were fixed in 2.5% Glutaraldehyde (0.1M cacodylate buffer, pH 7.2) and Karnovsky's fixative solution, and decalcified with 0.1M disodium ethylene diaminetetraacetate for 3 weeks. Tissue embeding was performed in paraffin and cut parallel to the surface of calvaria. Section in 7${\mu}m$ thickness of tissue was done and stained with Hematoxylin-Eosin. All the specimens were observed under the light microscopy. The following results were obtained. 1 . During the whole period of experiment, fibrous connective tissue was revealed at 1week after surgery which meant rapid soft tissue recovery. The healing rate of defected area into new bone formation of the test group was observed more rapid tendency than other two groups. 2 . The sequence of healing rate of bone defected area was as follows ; test group, positive control, negative control group. 3 . During the experiment, an osteoclastic cell around preexisted bone was not found. New bone formation was originated from the periphery of the remaing bone wall, and gradually extended into central portion of the bone defect. 4 . The biodegradable barrier membrane was observed favorable biocompatibility during this experimental period without any other noticeable foreign body reaction. And mineralization in the newly formed osteoid tissue revealed relatively more rapid than other group since early stage of the healing process. Conclusively, the cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of regeneration of artificial bone defects of alveolar bone. This study thus demonstrates a tissue-engineering the approach to the repair of bone defects, which may have clinical applications in clinical fields of the dentistry including periodontics.

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A Study on the Residual Leakage Field in the Surface Defect of Alloy Steel (합금강 표면결함의 잔류누설자장에 관한 연구)

  • Lee, I.S.;Park, U.S.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.9 no.1
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    • pp.22-29
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    • 1989
  • The important point of MPI is the analysis of leakage field in the defective regions. The analysis of leakage field depends on many factors such like geometry and character of defect. In general the calculation of magnetic leakage fields arising from such defects presents an extremely complicated mathematical problem and is practically insoluable, since the inhomogeneities have complex geometrical shapes and may differ in physical nature. Therefore, this paper describes Hall probe measurements of residual leakage field around artificial flaws in alloy steel bar, and shows how the results to recent developments in 2D dipole and analytic models of the magnetic field defect interaction.

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The effect of the surface defect from micro-hole for fatigue strength (피로강도에 대한 표면미소 결함의 영향)

  • 오환섭
    • Journal of the korean Society of Automotive Engineers
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    • v.11 no.1
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    • pp.51-56
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    • 1989
  • This study was performed to investigate the effect of defect on fatigue strength under the stress of rotary bending. The specimens were made of low carbon steel having artificial microholes, namely, single micro-hole and two adjacent micro-holes as natural defects, and the effects of the diameter of hole and the distance between the holes on fatigue strength have been investigated. The obtained result can be summarized as follows: 1, The critical defect means the largest size of defect that does not affect fatigue limit, and correspondes to the size of defect leading to final fracture under fatigue limit of smooth specimen. The size of defect which has an effect on fatigue limit is larger than that of critical defect. 2, The defect larger than the critical defect affects fatigue strength for as a kind of size effect, and the physical meaning of size effect of defect is considered same as the one of notch effect.

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Non-contact critical current measurement of superconducting coated conductor using Hall Probe (Hall Probe를 이용한 초전도선재의 비접촉 임계전류 측정 방법)

  • Kim, Ho-Sup;Oh, Sang-Soo;Lee, Nam-Jin;Ha, Dong-Woo;Baik, Seung-Kyu;Ko, Rock-Kil;Ha, Hong-Soo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.03b
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    • pp.12-12
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    • 2010
  • The hall probe measurement system was used to measure the critical current distribution of superconducting coated conductor. The system consists of reel to reel moving apparatus, 7 array hall probe, a rotary encoder and permanent magnet. The magnetic field profile across the width of superconducting coated conductor using Bean's critical state model was calculated. The effect of various parameters of the formulas on the magnetic field distribution and the effect of shape and size of artificial defects, which were formed on the surface of SmBa2Cu3O7-d(SmBCO) coated conductor using laser marking system, on the hall probe magnetic field signal of the hall probe measurement system was investigated.

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Comparison test for PD measuring about UHF-sensor and HFCT-sensor in cable joint-box of MV grade (MV급 케이블 중간접속부의 PD 측정을 위한 UHF-센서와 HFCT-센서의 비교시험)

  • Shin, Dong-Hoon;Lee, Yong-Sung;Kim, Jung-Yoon;Yang, Jong-Seok;Park, Noh-Joon;Park, Dae-Hee
    • Proceedings of the KIEE Conference
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    • 2006.07c
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    • pp.1511-1512
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    • 2006
  • In this paper, we measured PD signals from artificial surface defects and corona discharges under laboratory circumstances like the fields using both HFCT-sensor and UHF-sensor for MV grade XLPE cable with AMP, spectrum analyzer and oscilloscope. Also, detected signals were analyzed via PRPDA system in this paper. As a result, in this case of HFCT-sensor, the amount of background noise are larger than as compared to UHF-sensor. There is needed some hardware or software or software algorithm removing background noise effectively for diagnosis the underground power transmission cable in the case of HFCT-sensor.

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Evaluation of Debonding Defects in Railway Concrete Slabs Using Shear Wave Tomography (전단파 토모그래피를 활용한 철도 콘크리트 궤도 슬래브 층분리 결함 평가)

  • Lee, Jin-Wook;Kee, Seong-Hoon;Lee, Kang Seok
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.26 no.3
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    • pp.11-20
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    • 2022
  • The main purpose of this study is to investigate the applicability of the shear wave tomography technology as a non-destructive testing method to evaluate the debonding between the track concrete layer (TCL) and the hydraulically stabilized based course (HSB) of concrete slab tracks for the Korea high-speed railway system. A commercially available multi-channel shear wave measurement device (MIRA) is used to evaluate debonding defects in full-scaled mock-up test specimen that was designed and constructed according to the Rheda 200 system. A part of the mock-up specimen includes two artificial debonding defects with a length and a width of 400mm and thicknesses of 5mm and 10mm, respectively. The tomography images obtained by a MIRA on the surface of the concrete specimens are effective for visualizing the debonding defects in concrete. In this study, a simple image processing method is proposed to suppress the noisy signals reflected from the embedded items (reinforcing steel, precast sleeper, insert, etc.) in TCL, which significantly improves the readability of debonding defects in shear wave tomography images. Results show that debonding maps constructed in this study are effective for visualizing the spatial distribution and the depths of the debondiing defects in the railway concrete slab specimen.

A STUDY ON THE ARTIFICIAL INTERPROXIMAL CARIES DETECTION WITH THE DIGITAL RADIOGRAPHY (디지털방사선촬영술을 이용한 인접면 치아우식증 진단에 관한 실험적 연구)

  • Kwon Ki Jeong;Hwang Eui-Hwan;Lee Sang Rae
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.24 no.1
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    • pp.85-94
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    • 1994
  • The purposes of this study were clinical comparison and evaluation between digital radiography and conventional radiography for the detection of artificial interproximal caries. Four freshly extracted, unrestored posterior teeth were obtained and caries was simulated by drilling semicircled defects with variable size at the interproximal surface of each tooth. The experiments were performed with IBM-PC/32 bit-DX compatible, video camera(VM-S8200, Hitachi Co., Japan), and color monitor(Multisync 3D, NEC, Japan). Sylvia Image Capture Board for the ADC(analog to digital converter) was used, and spatial resolution was 512 × 480 with 256 gray levels. The obtained results were as follows: 1. At the condition of under exposure, the radiographs were superior to the digital images in readability. Also, as the size of the artificial lesion was increased, readability of the radiographs was elevated. 2. The digital images were superior to the radiographs in readability especially under over exsposure. 3. As the exposure time and size of lesion was increased, the gray level of region of interest of the digital image was decreased proportionally. 4. As the F-value of average gray level of region of interest at individual exposure time and size of lesion, gray level of the all lesion sizes was decreased in comparison with that of the normal enamel.

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Histologic Study Of Different Bioceramic Implants In Intrabony Defects (골내낭에 매식된 수종의 생체요법재료에 대한 조직학적 연구)

  • Lee, Chul-Woo;Choi, Sang-Mook;Han, Soo-Boo;Park, Sang-Hyun;Kim, Hyeon-Jong
    • Journal of Periodontal and Implant Science
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    • v.26 no.1
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    • pp.27-46
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    • 1996
  • The purpose of this study was designed to compare with the effects of 4 different surface active bioceramics on the healing process of alveolar bone defects in dogs. Artificial alveolar bone defects depth 4-6mm, width 3-4mm) were created with # 6 round bur at interproximal areas of maxillary canine, maxillary 2nd premolar, mandibular canine, and mandibular 3rd premolar. porous hydroxyapatite(Interpore $200^R$) , 45S5 bioglass, CJ4/lOC crystalline glass, and JJ crystalline glass were implanted in intrabony defects randomly. Experimental groups were divided into 4 categories according to its implant material. After implantation, all groups were examined postoperatively 4 weeks to 12 weeks. 3 dogs was selected randomly and sacrificed after vascular perfusion with 2.5% glutaraldehyde at every 4 weeks. Tissue blocks with surroundig alveolar bone and soft tissues were removed and immersed in formaldehyde/glutaraldehyde fixative. After 20 weeks decalcification with EDTA and formic acid, sections were made and observed under light microscope and transmission electron microscope. In all experimental groups, the encapsulation of inactive connective tissue was observed around graft particles in 4 weeks. As time elapsed, the thickness of surrounding connective tissue was decreased. Osteoconductive bone growth pattern was seen apparently in all groups. CJ4/lOC crystalline glass showed the most active bone formation until 8 weeks. 45S5 bioglass was, however, the most active in new bone formation at 12 weeks. Though there was difference in resorption rate among grafting materials, the size of graft particles was decreased gradually. 45S5 bioglass was resorbed faster than the others. On the other hand, porous hydroxyapatite was degraded most slowly. Phagocytosed particulate matters was observed in the cytoplasm of multinuclear multinuclear giant cell and macrophage under transmission electron microscope. The results suggested suggested that 45S5 bioglass and CJ4/lOC crystalline glass may have some enhanced reparative potential when compared to porous hydroxapatite in the treatment of periodontal defeds. JJ crystalline glass reguires a further investigation of the safety of its use.

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Integrity Evaluation of Railway Bogie Using Infrared Thermography Technique (적외선 열화상 기술을 이용한 철도차량 대차 건전성 평가)

  • Kim, Jeong-Guk
    • Journal of the Korean Society for Nondestructive Testing
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    • v.31 no.2
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    • pp.144-149
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    • 2011
  • The lock-in thermography was employed to evaluate the integrity of railway bogies. Prior to the actual application on railway bogies, in order to assess the detectability of known flaws, the calibration reference panel was prepared with various dimensions of artificial flaws. The panel was composed of structural steel, which was the same material with actual bogies. Through lock-in thermography evaluation, the optimal frequency of heat source was determined for the best flaw detection. Based on the defects information, the actual defect assessments on railway bogie were conducted with different types of railway bogies, which were used for the current operation. In summary, the defect assessment results with thermography method showed a good agreement as compared with the conventional inspection techniques. Moreover, it was found that the novel infrared thermography technique could be an effective way for the inspection and the detection of surface defects on bogies since the infrared thermography method provided rapid and non-contact mode for the investigation of railway bogies.

Detectability of Pore Defect in Wind Turbine Blade Composites Using Image Correlation Technique (이미지 상관 기법을 이용한 풍력 발전 블레이드용 복합재료의 기공 결함 검출능)

  • Kim, Jong Il;Huh, Yong Hak;Lee, Gun Chang
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.37 no.10
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    • pp.1201-1206
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    • 2013
  • Defects that occur during the manufacturing process or operation of a wind turbine blade have a great influence on its life and safety. Typically, defects such as delamination, pore, wrinkle and matrix crack are found in a blade. In this study, the detectability of the pores, a type of defect that frequently occur during manufacturing, was examined from the full field strain distribution determined with the image correlation technique. Pore defects were artificially introduced in four-ply laminated GFRP composites with $0^{\circ}/{\pm}45^{\circ}$ fiber direction. The artificial pores were introduced in consideration of their size and location. Three different-sized pores with diameter of 1, 2 and 3 mm were located on the top and bottom surface and embedded. By applying static loads of 0-200 MPa, the strain distributions over the specimen with the pore defects were determined using image correlation technique. It was found the pores with diameter exceeding 2 mm can be detected in diameter.