• 한국약용작물학회지
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• 제16권5호
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• pp.287-293
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• 2008

This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about $17.0\;mm{\times}4.0\;mm$ and pistils and stamens had the same length of 15.0mm at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.

• 식물병연구
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• 제12권3호
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• pp.235-239
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• 2006

Since the cultivation of resistance cultivar is essential for organic agriculture, Phytophthora late blight resistance of 16 advanced potato clones obtained from the potato breeding laboratory at the National Institute of Highland Agriculture was evaluated. Resistance of the clone was examined through artificial inoculation in the laboratory and in the field. Dominant isolates of the pathogen in Gangwon province produced abundant sporangia on leaves of most clones showing susceptibility. The number of sporangia ranged from $10^{7{\sim}8}sporangia/ml$ in the susceptible clones at 7 days after inoculation. However, one clone is resistant in organic farming fields. Disease incidence was 2.3% using the resistant clone. The area under disease progress curve(AUDPC) was 75.5. Contrarily, disease incidence of the susceptible variety was 100% and the AUDPC was 1773.5 during the same cultivation period. The resistance clone named as 'Haryeong' is considered suitable for organic potato cultivation in Gangwon province in Korea.

• Journal of the Korean Wood Science and Technology
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• 제46권5호
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• pp.437-448
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• 2018

본 연구는 태백산맥을 기준으로 영동(고성군), 영서(홍천군), 영남(봉화군) 세 지역 간 소나무의 재질차이의 유무를 알기위해 물리적 특성을 조사하였다. 기건함수율 및 생재함수율(인위적)의 산지 간 차이를 보이지 않았다. 비중은 심재부와 변재부에서 봉화군 소나무가 두 산지보다 높았으며, 고성군과 홍천군 소나무 사이에는 심재부에서는 고성군 소나무가 높고 변재부에서는 홍천군 소나무가 고성군 소나무 보다 높게 나타났다. 심재부의 비중이 높게 나타난 고성군 소나무가 강도적으로 홍천군 소나무보다 클 것으로 판단된다. 수축율은 방사방향의 경우 산지 간 차이가 없고, 접선방향의 경우는 가도관장이 긴 봉화군 소나무가 높게 나타났다. 흡습율은 산지 간 차이가 거의 없는 것으로 나타났다. 이 결과로 소나무를 효율적으로 사용할 수 있고, 육종적으로 좋은 재질의 품종생산에도 도움이 될 것으로 생각한다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권1호
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• pp.19-25
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• 2006

The effect of vitamin E supplementation on plasma ${\alpha}$-tocopherol level, total antioxidant level and reproductive performance in Murrah buffaloes was studied during periparturient period. Twenty-four advance pregnant buffaloes were randomly divided into four equal groups as $T_1$, $T_2$, $T_3$ and $T_4$ and were supplemented with 0, 1,000, 1,500 and 2,000 IU of ${\alpha}$-tocopheryl acetate (Merck) from 60 days prepartum to 30 days postpartum and 0, 500, 750 and 1,000 IU from 30 to 60 days postpartum, respectively. Blood samples were collected at -60, -45, -30, -15, -7, 0, 7, 15, 30 and 60 days of parturition and were analyzed for plasma ${\alpha}$-tocopherol and total antioxidant activity (TAA). The intake of DM, CP and TDN did not vary among different groups. Plasma ${\alpha}$-tocopherol and TAA around parturition (-7 to 15 day) in $T_3$ and $T_4$ were significantly higher than the control group. There was 17% reduction in retention of fetal membranes (RFM) and metritis in $T_4$ than control. The post partum estrus interval averaged 58.00, 55.33, 51.83 and 43.00 days in $T_1$, $T_2$, $T_3$ and $T_4$ respectively. There was significant reduction in days open in both $T_3$ and $T_4$ in comparison to $T_1$ group (127,130 Vs.146). All the vitamin E supplemented groups showed reduction in days open than their previous lactation performance. Supplementation of vitamin E at $1,500IU\;d^{-1}$ from 60 day prepartum to 30 day post partum to buffaloes exhibited beneficial effect on plasma ${\alpha}$-tocopherol level and TAA around parturition and continuation of its supplementation at $1,000IU\;d^{-1}$ from 30 to 60 days of lactation improved post partum reproductive performance of buffaloes.

• 한국습지학회지
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• 제7권4호
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• pp.81-88
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• 2005

2001년부터 2004년동안 4년간에 걸쳐 조사된 태화강하류의 겨울철새는 11목 21과 58종 최대 출현개체수는 14,034개체이다. 연도별로 출현 사항을 보면 종수의 경우 2001년 48종에서 2004년 31종으로 매년 줄어들고 있다. 한편 개체수의 경우 2002년에 11,991개체로 가장 많이 조사되었고 2004년이 3,476개체로 가장 적게 조사되었다. 5% 이상의 우점종은 흰죽지 Aythya ferina(6,946개체), 떼까마귀 Corvus frugilegus(1,643개체), 붉은부리갈매기 Larus ridibundus(1,193개체), 괭이갈매기 Larus crassirostris(805개체)로서 이들 4종의 개체수 합(10,587개체)이 전체 개체수의 75.4%를 차지한다. 태화강에 도래하는 조류를 보호하기 위해서는 강변 가까운 곳에 조류들의 먹이장소 조성, 쉼터 등 완충지대를 만들어주고 도로와 고수부지 사이는 수목대 또는 차광대와 방음벽을 설치할 필요가 있다. 무엇보다도 시민들의 철새에 대한 보호 의지가 강해야 한다. 또한 철새들이 많이 도래하는 태화강과 동천의 합수부 일대, 조류들의 번식지와 잠자리로 이용하는 대밭 지역($2{\times}0.1km^2$)의 서식지 보호 및 관리방안이 필요하다.

• 식물병연구
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• 제11권2호
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• pp.106-114
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• 2005

To breed powdery mildew resistant varieties, 104 accessions of Cucurbita spp. germplasm were evaluated. Powdery mildew resistance and horticultural characteristics of the accessions were investigated in the field conditions. C. martinezii was selected for high resistance to powdery mildew in the field. The growth of powdery mildew pathogen and response of leaf surface tissue to the pathogen were observed after artificial inoculation to resistant C martinezii and susceptible C. moschata cv. 'Wonye No.402'. Mycelium growth was very clearly different between resistant C. martinezii and susceptible C moschata cv. 'Wonye No. 402' at 5 days after inoculation at the adult stage. Interspecific hybridization was attempted to transfer powdery mildew resistance gene(s) from C. martinezii to C. moschata. A single dominant gene action was postulated as deter mining powdery mildew resistance at the seedling stage. But for the effective selection of powdery mildew resistant individuals, it was established that adult plant resistance should be confirmed in the field conditions after the seedling test. Through the seedling and field tests, C. moschata lines resistant to powdery mildew and superior to horticultural characteristics, were selected after 2 backcrossing and 3 selfing generations. The fruit characteristics of newly developed lines were similar to those of the recurrent parent, 'Jaechenjarae'.

• 대한수의학회지
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• 제39권1호
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• pp.111-117
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• 1999

A nation wide sero-epidemiological survey of porcine reproductive and respiratory syndrome(PRRS) was carried out to analyze the current status of the PRRS virus infections in the field using the indirect immunofluorescent antibody assay(IFA) with the field isolate PL96-1. Since the first report of the antibody detection to PRRSV in 1993, the prevalence of seropositive pigs has increased dramatically and the data indicate that over 21% of the pigs and around 60% of the farms showed seropositives to the PRRS virus. A slightly higher positive rate was recognized in breeders than fattenings and it might be due to the higher age at the time of testings. No significant regional differences were detected in the sero-epidemiological survey. Higher sero-positive rate in growers indicates that PRRSV infection in the field was common after weaning(around 40 days). However, the number of seropositive pigs were declined in fattening pigs. Sows showed around 26% of sero-positive rate that there is a higher chance of continuous virus circulation in the infected farms. Low rate of sero-positivity in boars(9.8%) implies that there is high demand in proper control measures to prevent virus spreading through breeding procedures such as natural or artificial insemination. Therefore it was concluded that PRRSV infection in domestic swine herds is endemic and the positive rate and economic loses will be increased by spontaneous infections in naive farms.

• 한국수산과학회지
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• 제1권1호
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• pp.9-18
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• 1968

1963년도부터 대하의 양식을 목적으로 부화시험을 통하여 다음과 같은 결과를 얻었다. 1. 방난은 야간에 이루어졌으며 대부분이 오후 10시경까지 완료되었으며 모하 한마리의 평균 방난수는 3만립에서 15만립 내외였다. 2. 방난된 난은 수온 $25\~26^{\circ}C$에서 18시간 경과되면 (부화수조내에서는 13시간)난내 Nauplius 기로 되었고 다시 40분이 지나면 제1기 Nauplius 기로 되었다. 3. 제1 Nauplius 기에서 Zoea 기까지의 기간을 약 4일간이였고, 제1 Zoea기에서 제1 Mysis기까지는 약 $3\~4$ 일간이였다. 4. 방난후 post larva까지는 $3\~4$주일간이 요했다. 5. 1966연의 변화율은 $1.4\~14.9\%$로서 평균 $6\%$였고, 1967연의 부화율은 $10\~35\%$로서 평균 $20\%$였다, 연구중 여러가지로 지도하여 주신 일본국 "KURUMA" PRAWN 양식주식회사 궁재광무박사 동회사 적영안언씨에 대하여서는 깊은 사의를 표하는 바이다.

• 생명과학회지
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• 제17권1호
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• pp.24-30
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• 2007

꽃치자(Gardenia jasminoides var. radicans)와 열매치자(C. jasminoides for. grandiflora)는 Gardenia속으로는 우리나라에 두 분류군밖에 없으며 열매치자는 약용, 식용, 꽃치자는 방향제로 쓰인다. 그런데 꽃이 지고 나면 형태적으로 구분이 거의 되지 않는다 ISSR분석으로 이들 종의 유전적 다양도와 집단구조를 실시하였다. 88개의 DNA 분절에서 한 분류군에만 나타나는 특이밴드가 탐지되었다. 한국의 세 야생 집단은 분리되어 있고 패치 분포를 보이지만 재배종에 비해 높은 유전적 다양성을 유지하친 있었다. 열매치자가 꽃치자보다 유전적 다양성이 높았으며 ISSR 마커로 이들 분류군이 잘 분리되었다. 또한 야생 집단이 재배 집단보다 다양성이 약간 높으나 유의성은 없었다. 이는 재배화과정에서 유전적 다양성의 일부 상실이 있었으나 인위적인 채취와 식재로 야생 집단과 재배 집단의 유전적 교류가 존재하였음을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1223-1232
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• 2017

Cordyceps militaris, a member of Ascomycota, a mushroom referred to as caterpillar Dong-chung-ha-cho, is commercially valuable because of its high content of bioactive substances, including cordycepin, and its potential for artificial cultivation. Cordycepin (3'-deoxyadenosine) is highly associated with the pharmacological effects of C. militaris. C. militaris is heterothallic in that two mating-type loci, idiomorph MAT1-1 and MAT1-2, exist discretely in two different spores. In this study, nine C. militaris strains were mated with each other to prepare newly bred strains that produced a larger amount of cordycepin than the parent strains. Nine strains of C. militaris were identified by comparing the internal transcribed spacer sequence, and a total of 12 single spores were isolated from the nine strains of C. militaris. After the MAT idiomorph was confirmed by PCR, 36 mating combinations were performed with six single spores with MAT1-1 and the others with MAT1-2. Eight mating combinations were successfully mated, producing stroma with perithecia. Cordycepin content analysis of all strains by high-performance liquid chromatography revealed that the KASP4-bred strain produced the maximum cordycepin among all strains, regardless of the medium and stroma parts. Finally, universal rice primer-PCR was performed to demonstrate that the bred strains were genetically different from the parental strains and new C. militaris strains. These results may be related to the recombination of genes during mating. The newly produced strains can be used to meet the industrial demand for cordycepin. In addition, breeding through mating suggests the possibility of producing numerous cordycepin-producing C. militaris strains.