• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.812-821
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• 2007

The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.464-469
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• 2011

The arsH gene is one of the arsenic resistance system in bacteria and eukaryotes. The ArsH protein was annotated as a NADPH-dependent flavin mononucleotide (FMN) reductase with unknown biological function. Here we report for the first time that the ArsH protein showed high ferric reductase activity. Glu104 was an essential residue for maintaining the stability of the FMN cofactor. The ArsH protein may perform an important role for cytosolic ferric iron assimilation in vivo.

• 농약과학회지
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• 제2권2호
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• pp.90-96
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• 1998

곤충 병원균을 미생물 살충제로서 이용하기 위한 대량배양 기술을 확립코자 미생물 살충제로 잠재성이 있는 Metarhizium anisopliae var. majus ARS 978의 대량배양을 위해 액체배양 조건을 확립하였다. 최적배양을 위한 온도와 pH는 각각 $28^{\circ}C$와 pH 7.0 이었다. M. anisopliae ARS 978의 균체량, 효소들의 활성 및 포자의 생성에 1.0 %(v/v) molasses, 1.0%(w/v) 주박, 0.3 %(w/v) $CaCO_{3}$가 가장 적합한 최적배지 조건이었다.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2019년도 하계 학술발표초록집
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• pp.130-143
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• 2019

• 대한치과교정학회지
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• 제23권4호
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• pp.447-454
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• 1993

Patient with skeletal class II relationship was treated with L-ARS. L-ARS is fixed functional appliance that could be effective in children and adolescent patient who don't wear activator. The following results were obtained ; 1) Growth of Mandible was stimulated and overjet was decreased, therefore coupling of anterior teeth was established with L-ARS which is fixed functional appliance. 2) These changes were accomplished with Mandibular skeletal growth with no effect on the Maxilla. 3) L-ARS was especially effective on patient who deny to wear the removable functional appliance.

• 음성과학
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• 제8권2호
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• pp.61-71
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• 2001

Speech material, which is collected from ARS(Automatic Response System), was analyzed and classified into disease and non-disease state. The material include 11 different kinds of diseases. Along with ARS speech, DAT(Digital Audio Tape) speech is collected in parallel to give the bench mark. To analyze speech material, analysis tools, which is developed local laboratory, are used to provide an improved and robust performance to the obtained parameters. To classify speech into disease and non-disease class, multi-layered neural network was used. Three different combinations of 3, 6, 12 parameters are tested to obtain the proper network size and to find the best performance. From the experiment, the classification rate of 92.5% was obtained.

• Environmental Engineering Research
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• 제15권1호
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• pp.15-21
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• 2010

This study was conducted to evaluated seawater bacteria and their seasonal characteristics in the arsenic contaminated coastal seawater of Yeosu Bay, the Republic of Korea. Arsenite-oxidizing bacteria play an important role in the seawater of the arsenic contaminated bay, with a variety of arsenic resistance system (ars) genotypes being present during summer. Specifically, Bacillus sp. strain SeaH-As22w (FJ607342), isolated from the bay, were found to contain the arsB, arrA and aoxR type operons, which are involved in arsenic resistance. The isolated bacteria showed relatively high tolerance to sodium arsenite (III; $NaAsO_2$) at concentrations as high as 50 mM. Additionally, batch seawater experiments showed that Bacillus sp. strain SeaH-As22w completely oxidized 1 mM of As (III) to As (V) within 10 days. Ecologically, the arsenic-oxidizing potential plays an important role in arsenic toxicity and mobility in As-contaminated coastal seawater of Yeosu Bay during all seasons because it facilitates the activity of Bacillus sp. groups.

• 유공압시스템학회:학술대회논문집
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• 유공압시스템학회 2010년도 춘계학술대회
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• pp.70-73
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• 2010

In order to provide theoretical analysis for the active roll stabilizer (ARS), the simulation model based on AMESim is developed in the paper. The simplified vehicle rolling motion model is derived firstly, and then the entire ARS control system model is constructed. Furthermore, the simulation is implemented to confirm the roll control effect. The simulation results show that the derived model can be used as theoretical analysis for developing components of ARS control system.

• Journal of Animal Science and Technology
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• 제44권1호
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• pp.87-94
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• 2002

본 실험은 육림부산물 발효사료의 사슴용 사료가치를 평가하기 위해 꽃사슴에게 육림부산물 사일리지(FBS), 칡 사일리지(ARS) 및 수입 갈잎건초(OLH)를 급여하여 건물채식량, 소화율 및 질소출납을 상호 비교하였다. 건물소화율은 ARS가 가장 높았고 OLH가 가장 낮아 처리구간에 유의성(P$<$0.05)이 인정되었다. 조단백질 소화율의 경우는 OLH구가 FBS와 ARS구에 비해 현저히 낮은(P$<$0.05) 소화율을 나타냈다. 조섬유 소화율의 경우는 FBS가 가장 높고 OLH가 가장 낮았다. 건물채식량은 OLH가 가장 높아 ARS와의 사이에는 유의성(P$<$0.05)이 인정되었으나 FBS와의 사이에는 인정되지 않았다. 그러나 체내에서 이용 가능한 가소화 건물채식량으로 환산했을 경우 차이가 거의 없었다. 체내에 축적된 질소와 섭취질소에 대한 축적질소의 비율은 처리구간에 유의성은 인정되지 않았으나 FBS가 가장 높아 체내 질소의 이용효율이 가장 좋았던 것으로 평가되었다. 결과적으로 육림부산물 사일리지는 다양한 사료자원으로 구성되어 사슴의 기호성이 양호하고 높은 소화율과 건물채식량 및 영양소의 체내 이용성을 나타내고 있어 사슴용 사료로서 충분한 가치를 갖고 있는 사료자원으로 평가되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1659-1666
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• 2000

Two experiments were conducted to determine the effects of Leucaena leucocephala (leucaena) and Gliricidia sepium (gliricidia) supplementation on intake, digestion, outflow rates, microbial protein yield and live-weight changes in sheep fed with ammoniated rice straw (ARS). In experiment 1, three rumen cannulated Japanese Corriedale wether (mean body weight of 35.6 kg) in $3{\times}3$ Latin Square Design were used. Animals were fed ad libitum ARS alone, or supplemented with 200 g of either leucaena or gliricidia. In experiment 2, twenty-four growing native Philippine sheep with average body weight of $13.5{\pm}0.25kg$ were used in a completely randomized design (CRD) and offered similar diets to those of experiment 1. Supplementation increased total dry matter intake and nutrient digestibility except for fiber (p<0.05) without affecting ARS consumption. Nitrogen balance revealed that absorbed and retained N was significantly higher in leucaena and gliricidia. The significant improvement in N utilization and more digestible OM intake brought about by the inclusion of leucaena and gliricidia to ARS resulted in increased (p<0.05) microbial N yield. Efficiency of microbial N supply in supplemented group was not significantly different, but higher (p<0.05) than the 24.92 g N/kg DOMR for ARS group. Liquid outflow rate was 7.8 and 6.8 %/h, while the solid phase of rumen digesta was 4.4 and 3.8 %/h for the leucaena and gliricidia group respectively, which were significantly higher than 5.30 and 2.50 %/h in the control diet. The increase in total DMI resulted to higher (p<0.01) growth performance and efficient feed utilization. Average daily gain (ADG) was 19.3, 34.6 and 33.9 g/d for the ARS, leucaena and gliricidia respectively. It is therefore concluded that addition of leucaena and gliricidia to ARS in could increase nutrient intake and digestibility, subsequently improving N utilization and livestock performance.