• 한국어류학회지
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• 제8권2호
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• pp.68-73
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• 1996

중국원산으로 한국에 도입된 초어(grass carp, Ctenopharyngodon idellua), 대두어(bighead carp, Aristichthys nobilis) 및 백련어(Silverer carp, Hypophthalmichthys molitrix)의 외부형태 및 염색체 특징에 대한 기본 정보를 얻기 위하여 본 연구를 하였다. 초어는 새파, 체측비늘, 지느러미들의 기조수와 계측치에서 대두어 및 백련어와 차이를 보였다. 대두어와 백련어는 복부에 용골과 외부 형태 등에서 유사했으나, 새파와 체측의 반문에서 쉽게 구분되었다. 그러나 염색체 수는 2n=48, arm number (fundamental number, NF)는 84로 모두 동일하였다. 연구된 3종의 염색체는 모두 metacentric chromosome이 10쌍, submetacentric chromosome이 8쌍, acrocentric 또는 telocentric chromosome이 6쌍으로 구성되어 있었다. 그리고 외부형태 형질, 국명 및 핵형의 유연관계에 대해서 논의하였다.

• BMB Reports
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• 제41권3호
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• pp.204-209
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• 2008

The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phyto-planktivorous silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ctenopharyngodon idellus). Using genome walker method, a 750 bp 5'-flanking region of the silver carp GPx gene was obtained, and several potential regulatory elements were identified in the promoter region of the GPx gene. The silver carp GPx gene was widely expressed in all tissues examined. Despite phylogenetic analysis, assigning this newly described carp GPx to the group of mammalian GPx2, the carp GPx seems more similar to GPx1 from a physiological point of view. The constitutive expression pattern of the three carp liver GPx gene, shows a positive relationship with their tolerance to microcystins.

• BMB Reports
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• 제39권4호
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• pp.346-354
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• 2006

The full-length cDNA of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) uncoupling protein 2 (UCP2) was obtained from liver. The grass carp UCP2 cDNA was determined to be 1152 bp in length with an open reading frame that encodes 310 amino acids. Five introns (Intron 3, 4, 5, 6 and 7) in the translated region, and partial sequence of Intron 2 in the untranslated region of grass carp UCP2 gene were also obtained. Gene structure comparison between grass carp and mammalian (human and mouse) UCP2 gene shows that, the UCP2 gene structure of grass carp is much similar to that of human and mouse. Partial UCP2 cDNA sequences of bighead carp (Aristichthys nobilis) and mud carp (Cirrhinus molitorella), were further determined. Together with the common carp (Cyprinus carpio) UCP2 sequence from GenBank (AJ243486), multiple alignment result shows that the nucleotide and amino acid sequences of the UCP2 gene, were highly conserved among the five major Chinese carps that belong to four subfamilies. Using beta-actin as control, the ratio UCP2/beta-actin mRNA (%) was determined to be $149.4{\pm}15.6$ (common carp), $127.4{\pm}22.1$ (mud carp), $96.7{\pm}12.7$ (silver carp), $94.1{\pm}26.8$ (bighead carp) and $63.7{\pm}16.2$ (grass carp). The relative liver UCP2 expression of the five major Chinese carps, shows a close relationship with their food habit: benthos and detrituseating fish (common carp and mud carp) > planktivorious fish (silver carp and bighead carp) > herbivorious fish (grass carp). We suggest that liver UCP2 might be important for Chinese carps to detoxify cyanotoxins and bacteria in debris and plankton food.