• Title/Summary/Keyword: Arbuscular mycorrhizal fungi (AMF)

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Effect of the different cover crop incorporation on glomalin-related soil protein and soybean and maize growth

  • Higo, Masao;Gunji, Kento;Isobe, Katsunori
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.344-344
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    • 2017
  • The glycoprotein known as glomalin-related soil protein (GRSP) is abundantly produced on the hyphae and spores of arbuscular mycorrhizal fungi (AMF) in soil and roots. GRSP play a decisive role in the soil aggregation, but GRSP was also sensitive to agricultural managements. Thus, our objectives were to assess the effect of different cover crop incorporation on the GRSP content in soil and growth of subsequent soybean and maize. Pot experiments with the incorporation of four cover crops were set up. The same amount (666g) of aboveground plant parts of wheat (AMF host), hairy vetch (AMF host), mustard (non-host) and rapeseed (non-host) was separately incorporated into soils. The aboveground plant parts and roots of soybean and maize were grown in each incorporated pots and sampled at 6 and 9 weeks after sowing. Our results showed that the different cover crops incorporation affected soil biological and chemical properties such as EC, $NO_3-N$ content, ${\beta}-glucosidase$ activity, alkaline phosphatase (ALP) activity and GRSP content. The soil EC and $NO_3-N$ content in the hairy vetch, mustard and rapeseed was higher compared to the wheat. The ${\beta}-glucosidase$ activity in the wheat and hairy vetch was significantly higher than that in the mustard and rapeseed, and the ALP activity in the wheat was significantly higher than that in the hairy vetch, mustard, and rapeseed. The GRSP content in the mustard and rapeseed was significantly lower than that of the hairy vetch and wheat. Moreover, The top dry weight and leaf area of soybean and maize in the hairy vetch at 6 weeks were significantly higher compared to the other treatments. Our results indicated that the incorporation of mustard and rapeseed may cause indirectly the decrease of GRSP content and soil enzyme activity in soil. One possible explanation for the decrease of GRSP in non-AMF host crop treatments may be the decrease of AMF density in the soil. AMF are not able to form a symbiotic relationship with Brassicaceae roots due to the release of anti-fungal compounds. This means the AMF may not be able to produce GRSP in the soil. However, the differences in the benefit of cover crop incorporation were shown only by a pot experiment. Comparative investigations of crop residue managements would be applied to both pot experiment and field study to clarify a better selection of cover crops in rotation to encourage GRSP production.

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Colonization Characteristics and Density of Arbuscular Mycorrhizal Fungi(AMF) in the Different Cultivated Grape Soils (재배방식이 상이한 포도 재배지 토양의 Arbuscular균근균 포자밀도와 감염특성)

  • Sohn, Bo-Kyoon;Cho, Ju-Sik;Liu, YanPeng;Lee, Do-Jin;Kim, Hong-Lim
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.6
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    • pp.476-481
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    • 2007
  • There was no difference between eco-friendly and conventional cultivated soils in the chemical properties. But $Av.P_2O_5$ contents in the eco-friendly cultivated soils were slightly higher than that of conventional cultivated soils. In the conventional cultivated soils, the coefficient of correlation between spore density and soil chemical properties such as pH, EC, OM, $Av.P_2O_5$, K${\surd}$(Ca+Mg) and CEC was $-0.48^*$, -0.05, $0.48^*$, -0.12, -0.13, 0.31 respectively. But, in the eco-friendly cultivated soils was $-0.68^*$, $0.69^*$, $0.96^{**}$, $0.75^*$, $0.63^*$, $0.92^{**}$ respectively. The spore density was 140 spores $30g^{-1}$ in the eco-friendly cultivated soils and 60 spores $30g^{-1}$ in the conventional cultivated soils. Infection ratio of intercellular hypha was higher than that of arbuscular and vesicular among the fungi structures within the root. Suncheon and Cheonan as eco-friendly cultivated soil were higher than GimJe and NamWon in infection ratio.

The spore densities of Arbuscular mycorrhizal fungi related to the Soils collected from Polluted and Unpolluted areas (오염지역과 비오염지역의 토양에 관한 Arbuscular mycorrhizal fungi의 포자밀도)

  • Shim, Jae-Ouk;Lee, Sang-Sun;Chang, Young-Soo
    • The Korean Journal of Mycology
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    • v.24 no.1 s.76
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    • pp.56-66
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    • 1996
  • Ecological variations of Arbuscular mycorrhizal (AM) fungi were determined from the soils collected from different sites of the polluted and unpolluted areas related to the soil pollutions. Average 24.5 spores $(per\;20g)^{-1}$ soil of AM fungal spore were counted from the 32 sites of soils collected from On-san (polluted), whereas average 4.1 spores $(per\;20g)^{-1}$ soil from the 18 sites of those from Mt. Kwanak (polluted); Average 23.6 spores $(per\;20g)^{-1}$ soil of AM fungal spore were also counted from the 30 sites of soils collected from Chung-mu (unpolluted), whereas average 15.8 spores $(per\;20g)^{-1}$ soil from the 14 sites of those from Mt. Chungwang (unpolluted).The spores of AM fungi were the species of Glomus, Gigaspora, Acaulospora and Scutellospora. Among the above four genera, the species of Glomus were observed to be more abundant than the other genera in the soils collected from On-san, Chung-mu and Mt. Chungwang whereas the species of Gigaspora in those from Mt. Kwan-ak. The parameters of soils measured showed some variations between the polluted and unpolluted areas; 12.9 to 16.4% in the soil moisture, 5.6 to 8.3% in the organic matter and 4.3 to 5.7 at soil pH (polluted to unpolluted areas, respectively). The soils collected, thereby, appeared to be more strongly acidic and also lower in the contents of soil moisture or organic matter at the polluted area than unpolluted area. Based on the ecological criteria, the species richness or species diversity had significant differences (p<0.05) between polluted and unpolluted area. The spore density of genus Glomus or Gigaspora was significantly different (p<0.05) among the soils of three different plant vegetations (conifer plants, broad leaf plants, and grass plants). Also, there were significant differences (p<0.05) in the species evenness or species diversity among the soils referring to three different plant vegetations. There was a direct relationship $(r^2=0.38)$ between soil moisture and organic matter measured from 94 soil samples. Since there was a direct relationship $(r^2=0.22)$ between organic matter and total spores, it seems to be likely to presume that mycorrhizal spores can be increased in proportion to enhanced organic matters in soils. The species richness or species diversity was inclined to increase in proportion to enhanced soil pH and total spores in soils.

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Effect of Functionally-strengthened Fertilizers on Garlic Growth and Soil Properties

  • Li, Jun-Xi;Wee, Chi-Do;Sohn, Bo-Kyoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.2
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    • pp.308-315
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    • 2011
  • Ammonium- and potassium-loaded zeolite (NK-Z) and other four kinds of environmental friendly fertilizers/agents were applied to characterize their effectiveness on garlic (Allium sativum L.) growth and soil amelioration. Selenium dioxide ($SeO_2$) and germanium dioxide ($GeO_2$) liquid treatments significantly increased selenium (Se) and germanium (Ge) contents in garlic stems, garlic cloves and clove peels. In soil treated with ZBFC, Se contents in garlic stems, cloves, and clove peels was 13.89-, 12.79-, and 10.96-fold higher, respectively, than in the controls. The inorganic contents of plants grown in soil treated with functional strengthened fertilizers were also higher than in plants grown in control soil. Soil treated with arbuscular mycorrhizal fungi (AMF) agents exhibited significantly greater spore density and root colonization rate than in untreated soil. The density of chitinolytic microorganisms in soil treated with colloidal chitin was also significantly higher than in untreated soil. The cation exchange capacities (CEC) in ZAFC-, ZBFC-, and ZBF-treated soils was 16.05%, 8.95%, and 8.80% higher than in control soil 28 weeks after sowing.

Korean-Style No-tillage Organic Agriculture on Recycled Ridge IV. Changes in Soil Microorganisms and Enzymes by Split Irrigation and Organic Matter Application in Organic Farming of Red Pepper in Plastic Film Greenhouse (두둑을 재활용한 한국형 무경운 유기 농업 IV. 분할관수와 유기물처리에 의한 시설 고추 유기재배 토양 미생물상과 토양 효소의 변화)

  • Yang, Seung-Koo;Shin, Kil-Ho;Song, Yong-Su;Kim, Kil-Yong;Jung, Woo-Jin
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.311-328
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    • 2017
  • This study was carried out to investigate the changes in soil microorganisms and soil enzymes by split irrigation and organic matter application under no-tillage green house conditions. Soil bacteria and fungi abundances were higher in soybean cake fertilizer than in the soil without the soybean cake fertilizer under whole quantity irrigation. Bacteria and fungi abundances in soil increased with increasing organic fertilizer application rate. Bacteria and fungi amount in the soil increased at half division irrigation in no-treatment of soybean cake fertilizer compared with whole quantity irrigation. Actinomycete amount in the soil decreased with increasing soybean cake fertilizer with whole quantity irrigation while clearly increased in no-treatment of soybean cake fertilizer. Actinomycete amount in soil clearly increased with increasing organic fertilizer input at half division irrigation. Chitinase activity in the soil decreased in soybean cake fertilizer with increasing organic fertilizer input, while increased in no-treatment of soybean cake fertilizer. Chitinase activity in the soil increased at half division irrigation compared with whole quantity irrigation regardless of soybean cake fertilizer input. ${\beta}$-Glucosidase activity in the soil was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. ${\beta}$-Glucosidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. ${\beta}$-Glucosidase activity in the soil clearly increased in no-treatment of soybean cake fertilizer at half division irrigation compared with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil was not significantly different at half division irrigation and whole quantity irrigation in organic fertilizer input, while increased at half division irrigation in no-treatment of soybean cake fertilizer. Acid phosphatase activity increased at standard level 66% in soybean cake fertilizer, while was not significantly different in no-treatment of soybean cake fertilizer. Spore density of Arbuscular Mycorrhizal Fungi (AMF) in the soil increased with increasing organic fertilizer input at whole quantity irrigation in no-treatment of soybean cake fertilizer, while decreased above the standard level 66% in organic fertilizer input. However, spore density of AMF in the soil was not significantly different in soybean cake fertilizer regardless of input amount of organic fertilizer. Root colonization rate of AMF in red pepper roots was not significant difference at two irrigations regardless of soybean cake input.