• 한국동물생명공학회지
• /
• 제37권4호
• /
• pp.239-245
• /
• 2022

Protein and peptide candidates are screened to apply therapeutic application as a drug. Ensuring that these candidates are delivered and maximized effectiveness is still challenging and a variety of studies are ongoing. As drug delivery system vehicles, cell-penetrating peptide (CPP) can deliver various kinds of cargo into the cell cytosol. In a previous study, we developed Ara27 CPP, which are a zinc knuckle family protein of Arabidopsis, and confirmed internalization in human dermal fibroblasts and human dental pulp stem cells at low concentration with short time treatment condition without any toxicity. Ara27, an amphipathic CPP, could be modified and utilized in the biomedical field excluding the risk of toxicity. Therefore, we would like to confirm the non-toxic induced penetrating ability of Ara27 in various cell lines. The purpose of this study was to screen the cell internalization ability of Ara27 in various cell lines and to confirm Ara27 as a promising core CPP structure. First, Ara27 was screened to confirm non-toxicity concentration. Then, fluorescence-labeled Ara27 was treated on human normal cell lines, cancer cell lines and animal cell lines to identify the cellular internalization of Ara27. Ara27 was well intracellular localized in all cell lines and the intensity of fluorescence was remarkably increased in time pass manner. These results indicate that Ara27 has the potential as a core structure for applications in various drug delivery systems.

• 대한본초학회지
• /
• 제27권3호
• /
• pp.1-6
• /
• 2012

Objectives : The present study was undertaken to evaluate whether Atractylodis Rhizoma Alba ethanol (ARA-E) extract, which is the pericarp of $Atractylodes$ $japonica$ Koidz. has an effect on collagen-induced arthritis (CIA) in mice. Methods : Male DBA/1J mice were induced by intradermal injection of bovine collagen-II in Freund's incomplete adjuvant (IFA). The CIA mice in the onset of arthritis were treated daily with oral administration of ARA-E extract at dose of 50 mg/kg/bw for 28 days. Arthritis index, histopathological changes and the levels of anti-type II collagen (CII) IgG and inflammatory cytokine, TNF-${\alpha}$ in sera of mice were measured to evaluate the antiarthritic effect of ARA-E. Results : ARA-E extract significantly decreased the arthritic scores and inhibited pathological changes of knee joint tissues in CIA mice. ARA-E extract also significantly decreased the serum levels of anti-CII IgG and TNF-${\alpha}$ in CIA mice. These results indicate that ARA-E extract may effectively prevent arthritic damages in CIA mice, at least partially, by inhibiting the production of autoantibodies and inflammatory cytokine. Conclusions : This studies suggest that ARA-E has a therapeutic potential in inflammatory joint diseases such as rheumatoid arthritis.

• 광물과 암석
• /
• 제35권1호
• /
• pp.13-24
• /
• 2022

기후 변화에 민감하게 반응하는 북극해는 지구 기후 변화의 흔적을 고스란히 간직하고 있으므로, 북극해 퇴적물 연구는 지구 기후 변화를 연구하는 데 매우 중요하다. 2016년에 수행된 아라온호의 ARA07C 북극해 탐사를 통하여 동시베리아해 연변부에서 획득한 ARA07C-St02B 중력 코어를 이용하여 퇴적학적, 광물학적, 지화학적 특성을 연구하고 이를 이용하여 기원지를 추정하였다. 코어 퇴적물은 색, 입도와 빙운쇄설물의 함량에 의하여 4개의 유닛으로 구분하였는데, 갈색층을 포함하는 유닛 1과 3은 회색층으로 구성된 유닛 2와 4보다 모래와 빙운쇄설물의 함량이 다소 높다. ARA07C-St02B 코어 주변에서 연구된 ARA03B-27 코어와 비교를 통하여 이들은 MIS (Marine Isotope Stage) 1부터 4시기에 퇴적된 것으로 여겨진다. 갈색층을 포함하는 유닛들과 회색층으로 이루어진 유닛 사이에는 벌크 광물 조성, 점토광물 조성, 지화학 조성에서 차이가 난다. 벌크 광물과 점토광물 특성은 조립질 퇴적물과 세립질 퇴적물 사이에 기원지가 다를 수 있음을 시사한다. 조립질 퇴적물은 대부분 랍테프해와 동시베리아해로부터 동시베리아 연안류나 추크치해로부터 보퍼트 환류를 따라 운반된 것으로 여겨지지만, 세립질 퇴적물은 대부분 동시베리아해, 추크치해, 보퍼트해로부터 해류에 의하여 운반된 것으로 추정된다. 유닛 1의 일부 조립질 퇴적물과 유닛 3의 세립질 퇴적물은 보퍼트해와 캐나다 북극 군도로부터 해류, 빙산 또는 해빙에 의하여 연구 지역에 퇴적된 것으로 판단된다. 잠재적인 근원지의 지화학 조성에 관한 자료를 획득하게 되면, 연구 지역의 코어 퇴적물의 기원지와 공급 방법에 대하여 좀 더 자세하게 규명될 수 있을 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제29권5호
• /
• pp.739-746
• /
• 2016

A total of 152 pig farms were randomly selected from the five provinces in South Korea. During the experiment, the average temperature and relative humidity was $24.7^{\circ}C$ and 74% in summer and $2.4^{\circ}C$ and 53% in winter, respectively. The correlation between floor space allowance (FSA) and productivity index was analyzed, including non-productive sow days (NPD), number of weaners (NOW), survival rate (SR), appearance rate of A-grade pork (ARA), and days at a slaughter weight of 110 kg (d-SW) at different growth stages. The objectives of the present study were i) to determine the effect of FSA on the pig productivity index and ii) to suggest the minimum FSA for pigs based on scientific baseline data. For the pregnant sow, NPD could be decreased if pregnant sows were raised with a medium level (M) of FSA (3.10 to $3.67m^2/head$) while also keeping the pig house clean which improves hygiene, and operating the ventilation system properly. For the farrowing sows, the NOW tended to decrease as the FSA increased. Similarly, a high level of FSA (H) is significantly negative with weaner SR of farrowing sows (p-value = 0.017), indicating this FSA tends to depress SR. Therefore, a FSA of 2.30 to $6.40m^2/head$ (very low) could be appropriate for weaners because a limited space can provide a sense of security and protection from external interruptions. The opposite trend was observed that an increase in floor space (> $1.12m^2/head$ leads to increase the SR of growing pigs. For the fattening pigs, H level of FSA was negatively correlated with SR, but M level of FSA was positively correlated with SR, indicating that SR tended to increase with the FSA of 1.10 to $1.27m^2/head$. In contrast, ARA of male fattening pigs showed opposite results. H level of FSA (1.27 to $1.47m^2/head$) was suggested to increase productivity because ARA was most affected by H level of space allowance with positive correlation ($R^2=0.523$). The relationship between the FSA and d-SW of fattening pigs was hard to identify because of the low $R^2$ value. However, the farms that provided a relatively large floor space (1.27 to $1.54m^2/head$) during the winter period showed d-SW was significantly and negatively affected by FSA.

• 대한인간공학회지
• /
• 제33권2호
• /
• pp.167-174
• /
• 2014

Objective: This research focuses on the working environment of office workers and aims to propose an ideal seating posture and chair design. Background: Generally, office workers having to seat in a fixed posture for long periods of time tend to suffer from an increased rate of musculoskeletal disorders. Method: The measurement and survey of 123 office workers on their working conditions and literature survey will lead to a suggestion of an ideal seating posture and workstation design theory. Results: Work environment satisfaction appeared low for chair and desks. Work-related pain over the last year was experienced among 47.2% of the respondents in shoulder (34.2%), neck (30.9%). Observation of working posture revealed that only 27.6% of workers utilize backrest during work. Conclusion: Office work embodies many situations where risks of musculoskeletal disorders may be high. Thus, the seated workplace must be set up to allow proper seating posture for workers. Application: The results of this study can be applied as baseline data for preventing musculoskeletal disorders of office workers.

• 광학세계
• /
• 통권144호
• /
• pp.31-38
• /
• 2013

한 일 광학산업 기술협력사업의 일환으로 한일산업기술협력재단과 한국광학기기산업협회가 주관한 기술연수에 참가했다. 필자는 광학이 아닌 전자공학을 전공했지만 광전자 회사를 다니는 연유로 이번 연수에 참가할 수 있는 기회를 얻었다. 연수에는 한국산업기술대학교의 조언정 교수를 포함해 광학업체 대표와 연구원 등 총 17명이 함께했다. 중소기업인들은 물론 대기업인 삼성전자 광학개발 담당자들도 참석해 폭넓은 의견을 나눌 수 있는 자리였다. 지난 1월 27일부터 31일까지 총 4박 5일 동안 참가한 이번 연수는 도쿄대학 광학 관련 연구소 방문 및 세미나, 광학 업체 오하라(OH ARA) 방문, 세계 최대 규모 나노기술 전시회인 '도쿄 나노테크 2013' 참관 등의 일정으로 진행됐다.

• Nonlinear Functional Analysis and Applications
• /
• 제27권1호
• /
• pp.169-187
• /
• 2022

There have been provided a surprisingly large number of summation formulae for generalized hypergeometric functions and series incorporating a variety of elementary and special functions in their various combinations. In this paper, we aim to consider certain generalized hypergeometric function ₃F₂ with particular arguments, through which a number of summation formulas for _p+1F_p(1) are provided. We then establish a power series whose coefficients are involved in generalized hypergeometric functions with unit argument. Also, we demonstrate that the generalized hypergeometric functions with unit argument mentioned before may be expressed in terms of Bell polynomials. Further, we explore several special instances of our primary identities, among numerous others, and raise a problem that naturally emerges throughout the course of this investigation.

• Journal of Gastric Cancer
• /
• 제16권3호
• /
• pp.152-160
• /
• 2016

Purpose: Endoscopic diagnosis of gastric cancer (GC) that emerges after eradication of Helicobacter pylori may be affected by unique morphological changes. Using comprehensive endoscopic imaging, which can reveal biological alterations in gastric mucosa after eradication, previous studies demonstrated that Congo red chromoendoscopy (CRE) might clearly show an acid non-secretory area (ANA) with malignant potential, while autofluorescence imaging (AFI) without drug injection or dyeing may achieve early detection or prediction of GC. We aimed to determine whether AFI might be an alternative to CRE for identification of high-risk areas of gastric carcinogenesis after eradication. Materials and Methods: We included 27 sequential patients with metachronous GC detected during endoscopic surveillance for a mean of 82.8 months after curative endoscopic resection for primary GC and eradication. After their H. pylori infection status was evaluated by clinical interviews and $^{13}C$-urea breath tests, the consistency in the extension of corpus atrophy (e.g., open-type or closed-type atrophy) between AFI and CRE was investigated as a primary endpoint. Results: Inconsistencies in atrophic extension between AFI and CRE were observed in 6 of 27 patients, although CRE revealed all GC cases in the ANA. Interobserver and intraobserver agreements in the evaluation of atrophic extension by AFI were significantly less than those for CRE. Conclusions: We demonstrated that AFI findings might be less reliable for the evaluation of gastric mucosa with malignant potential after eradication than CRE findings. Therefore, special attention should be paid when we clinically evaluate AFI findings of background gastric mucosa after eradication (University Hospital Medical Information Network Center registration number: UMIN000020849).

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2022년도 추계학술대회
• /
• pp.41-41
• /
• 2022

제주 내 크게 6개 지역에 흩어져 분포하고 있는 곶자왈은 화산활동으로 형성되었으며 제주 산림생물자원의 46%가 분포하여 생물종다양성이 매우 높은 것으로 알려져 있다. 곶자왈은 자갈과 바위로 이루어져 있으며 토양이 적게 발달하여 건조에 직면할 가능성이 크다. 본 연구는 곶자왈에서 서식하는 멸종위기종이자 산림청에서 희귀식물로 지정한 개가시나무(Quercus gilva)와 같은 속이면서 공존하고 있는 종가시나무(Quercus glauca)의 건조 내성에 대한 반응을 알아보고자 실내에서 생리 반응을 연구하였다. 온도(22±2.94℃)와 토양 양분(10%)은 자생지의 값을 기준으로 처리하였으며 수분 처리는 토양을 채운 화분에 물이 화분 밑으로 새어나가기 직전까지 포장용수량 700mL를 공급한 후 27일간 단수하였다. 단수기간에 수분함량의 변화는 토양수분함량측정기(Watch Dog)를 설치하여 데이터를 수집하였다. 항목은 토양수분함량에 따른 광합성률, 증산률, 수분이용효율, 기공전도도, Fv/Fm, Fo, Fm, 엽록소함량을 측정하였다. 토양수분함량은 25, 27일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. 광합성률은 27일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. 증산률은 4, 5, 11, 14, 25, 27일째에 개가시나무가 종가시나무보다 낮았다. 수분이용효율은 13일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. 기공전도도는 3, 9일째에 개가시나무가 종가시나무보다 높았다. Fv/Fm은 22, 27일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. Fo은 3, 14일째를 제외한 기간에 개가시나무가 종가시나무보다 높았다. Fm과 엽록소함량은 기간에 개가시나무가 종가시나무보다 높았다. 이와 같은 실험 결과로 보았을 때 개가시나무가 종가시나무보다 건조 내성에 강한 것으로 판단된다.

• Journal of Microbiology and Biotechnology
• /
• 제27권10호
• /
• pp.1877-1884
• /
• 2017

Mesenchymal stem cells (MSCs) have been suggested as a primary candidate for cell therapy applications because they have self-renewal and differentiation capabilities. Although they can be expanded in ex vivo system, clinical application of these cells is still limited because they survive poorly and undergo senescence or apoptosis when transplanted and exposed to environmental factors such as oxidative stress. Thus, reducing oxidative stress is expected to improve the efficacy of MSC therapy. The milk protein lactoferrin is a multifunctional iron-binding glycoprotein that plays various roles, including reduction of oxidative stress. Thus, we explored the effect of lactoferrin on oxidative stress-induced senescence and apoptosis of human MSCs (hMSCs). Measurement of reactive oxygen species (ROS) revealed that lactoferrin inhibited the production of hydrogen peroxide-induced intracellular ROS, suggesting lactoferrin as a good candidate as an antioxidant in hMSCs. Pretreatment of lactoferrin suppressed hydrogen peroxide-induced senescence of hMSCs. In addition, lactoferrin reduced hydrogen peroxide-induced apoptosis via inhibition of caspase-3 and Akt activation. These results demonstrate that lactoferrin can be a promising factor to protect hMSCs from oxidative stress-induced senescence and apoptosis, thus increasing the efficacy of MSC therapy.