• Title/Summary/Keyword: Aquatic Recovery

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Recovery of aquatic insect communities after a catastrophic flood in a Korean stream

  • Lee, Hwang-Goo;Bae, Yeon-Jae
    • Animal cells and systems
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    • v.15 no.2
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    • pp.169-177
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    • 2011
  • In August 2002, a heavy rainfall (445 mm in total for 5 consecutive days) resulted in a catastrophic flood, and it completely washed away the benthic fauna from the mainstream channel of the Gapyeong stream, a typical mid-sized stream in the central Korean peninsula. This study was to investigate the recovery patterns of aquatic insect communities that were damaged by the flood. Aquatic insects were sampled quantitatively using a Surber sampler ($50{\times}50$ cm, 1 riffle and 1 pool/run habitats per site) from three sites (4th-6th order) of the Gapyeong stream prior to 2000 and seasonally after the flood event from 2003 to 2006. Before the flood in the reference year (2000), a total of 77 species of aquatic insects were collected, whereas after the flood 47 species (2003), 51 species (2004), 64 species (2005) and 55 species (2006) were collected from the whole sampling sites. The aquatic insect density decreased to 26.85% (2003), 90.25% (2004), 52.53% (2005) and 54.95% (2006) of that recorded in the reference year. Although approximately 70% of the aquatic insect fauna has recovered since the flood event, the species composition in the most recent year differed substantially (similarity ca. 50%). On the other hand, the compositions of functional groups have not significantly changed. Aquatic insect communities at the riffle sites were affected more profoundly than those at the pool/run sites. The aquatic insect communities at the upstream site recovered more rapidly than those at the downstream sites.

Effects of Pain and Functional Recovery when Low Frequency Electrical Stimulation and Aqua-exercise Applied to Sciatic Nerve Injured Rats (좌골신경손상 백서의 통증과 기능회복에 저주파 전기자극과 수중운동이 미치는 효과)

  • Kim, Young-Eok
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.6 no.1
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    • pp.17-30
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    • 2008
  • This study was investigated the effects of pain and functional recovery when low frequency electrical stimulation and aquatic exercise applied to sciatic nerve injured rats. The rats were assigned into four groups; Group I(n=20, control group), Group II(n=20, low frequency electrical stimulation group), Group III(n=20, aquatic exercise group), Group (n=20, applied low frequency electrical stimulation and aquaatic exercise group). Each IV group measured hot plate examination, sciatic nerve functional index(SFI), c-fos.. In hot plate examination, group II, IV showed effect than group Iat 14 days after injured(p<0.01) and group III, Ⅳ showed effect than group I at 21, 28 days after in-jured(p<0.01, p<0.001). In SFI, group II, III, IV showed effect II, III, IV than group I and group IV showed effect than group II at 14, 21 days after injured(p<0.001). group II, III, IV showed effect than group I at 28 days after injured(II = p<0.01, III and IV = p<0.001). Effects of pain and function recovery when low frequency electrical stimulation and aqua-exercise applied to sciatic nerve injured rats, group Ⅳ were most effected to sciatic nerve injured rats. As well as group II and III were effected to sciatic nerve injured rats.

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Analysis of Penicillin Antibiotics in Aquatic Products (수산물 중 penicillin계열 항생제의 분석)

  • Bae, Jin-Han;Kim, Bo-Mi;Choi, Mi-Sun;Roh, Hye-Jin;Park, Mi-Jung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.6
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    • pp.629-636
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    • 2010
  • A simple, rapid method for determining amoxicillin (AMO) and ampicillin (AMP) in aquatic products (flatfish, salmon, shrimp, tilapia, and yellow croaker) was evaluated. For quantification, the AMO and AMP ions at m/z 348.9 and 105.9, respectively, were selected. The limit of detection (LOD) and limit of quantification (LOQ) for detecting AMO were 0.09 and $0.25\;{\mu}g/L$, respectively. The respective values for AMP were 0.02 and $0.05\;{\mu}g/L$. After $100\;{\mu}g/L$ AMO treatment, the level decreased 10% after 7 days at $5^{\circ}C$, while it decreased 20% at $25^{\circ}C$. After 7 days, 94.9.100% of $100\;{\mu}g/L$ AMP remained after storage at $5^{\circ}C$, while 62.3.100% remained after storage at $25^{\circ}C$. Using the food code method, the recovery of AMO ranged from 64.1.92.0% and that of AMP from 44.8.86.2%. With a protein centrifugation method, the recovery of AMO ranged from 39.8.87.9% and that of AMP from 78.0.98.1%. With liquid-liquid extraction, the recovery of AMO ranged from 36.5.88.3% and that of AMP from 31.8.75.1%.

Determination of Emamectin Benzoate in Eel, Halibut, and Shrimp Using QuEChERS-EDTA and LC-MRM

  • Cho, Ha Ra;Kim, Dong Yoon;Lee, Hyo Chun;Han, Sang Beom;Shin, Ho-Chul;Choi, Yong Seok
    • Mass Spectrometry Letters
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    • v.9 no.4
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    • pp.100-104
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    • 2018
  • As a part of efforts to establish the positive list system (PLS) in South Korea, a method to determine residual emamectin benzoate (EB) in various aquatic products using QuEChERS-EDTA and LC-MRM was developed. The developed method was validated in the aspects of specificity, linearity (correlation coefficient of at least 0.996), sensitivity (the limit of detection and the lower limit of quantitation ${\leq}5ng/g$), recovery (the recovery range of 87.4 and 96.2), and precision (the relative standard deviation of recovery between 0.9 and 13.5). Additionally, the validated method was successfully applied for monitoring EB contamination in eel, halibut, and shrimp collected from local food markets. To our knowledge, the present method is the first one to determine residual EB in various aquatic products at the level satisfying the PLS and could contribute to the establishment of the PLS in South Korea.

Effects of Recovery of Underwater Walking and Recovery of Underwater Sitting on Growth Hormone, Testosterone, Blood Lactate, Double product and Muscle Pain after Resistance Exercise (수중걷기회복과 수중앉기회복이 저항운동 후 성장호르몬, 테스토스테론, 혈중젖산농도, 심부담도 및 근통증에 미치는 영향)

  • Park, Jun-Sik;Jang, Tae-Soo;Jeong, Hwan-Jong;Kim, Ki-Hong
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.6
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    • pp.1646-1658
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    • 2020
  • In order to investigate the changes in growth hormone, testosterone, blood lactate, double product, and pain, this study conducted intensive weight training and circuit weight training with 60% intensity of 1RM for 7 men who had more than 6 months of resistance exercise and then performed Underwater Walking and Underwater Sitting with underwater recovery. Growth hormone was high in all exercise forms and recovery methods in order of after exercise, after recovery, and after stability, and testosterone was high in order of after exercise, after recovery, and stability. Blood lactate was higher in all exercise forms and recovery methods in order of after exercise, after recovery, and after stabilization, and dynamic recovery after concentration resistance exercise was lower than static recovery. Double product was higher in all types of exercise and recovery methods in order of after-exercise, after-recovery, and stability. Muscle pain decreased in the order of exercise, recovery, 24 hours, 48 hours, and 72 hours in all exercise forms and recovery methods. In the water environment, dynamic recovery is considered to be more effective in improving muscle fatigue than static recovery.

A Simple Method for Determining Residual p-arsanilic Acid in Aquatic Products using EDTA-Assisted Solvent Extraction and LC-MRM

  • Min Kyeong Kwon;Ye Bin Shin;Young Min Kim;Jin Hwan Kim;Ji Seong Kim;Yong Seok Choi
    • Mass Spectrometry Letters
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    • v.14 no.2
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    • pp.36-41
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    • 2023
  • A simple method was developed to determine residual p-arsanilic acid (ASA), an organo-arsenic compound used as a feed additive, in aquatic products (eel, halibut, and shrimp) using EDTA-assisted solvent extraction and LC-MRM. The method was successfully validated in terms of specificity, linearity (coefficient of determination ≥ 0.995), accuracy (recovery or R, 72.72-78.73%), precision (the relative standard deviation of R, 2.08-6.98%), and sensitivity (the lower limit of quantitation, 5 ppb) according to CODEX guidelines (CAC-GL 71-2009). The use of EDTA in the extraction solvent and water with a suitable pH modifier as the reconstitution solvent may be the key factors for successful results. This is the first method that can be used for monitoring residual ASA in aquatic products using LC-MRM and could contribute to establishing a better aquatic product safety management system.

Comparative Efficacy of 5 Anesthetic Agents in the Glass Catfish Kryptopterus vitreolus (유리메기(Kryptopterus vitreolus)에 대한 최적 마취제 선정을 위한 연구)

  • Lee, Hyo Bin;Jung, Hyo Sun;Ko, Min Gyun;Kim, Dong Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.824-828
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    • 2017
  • We investigated the anesthetic effects of MS-222 (tricaine methanesulfonate), clove oil, 2-phenoxyethanol, $NaHCO_3$, lidocaine-HCl and lidocaine-$HCl/NaHCO_3$ in the glass catfish Kryptopterus vitreolus. Based on the efficacy criteria of complete anesthetic induction from 60 s to 120 s, recovery within 300 s, the lowest effective concentrations at $24^{\circ}C$ were determined to be 60 ppm (induction $82.8{\pm}17.6s$, recovery $80.2{\pm}34.7s$) for MS-222, 40 ppm (induction $70.5{\pm}8.2s$, recovery $83.4{\pm}17.7s$) for clove oil, 250 ppm (induction $64.3{\pm}24.0s$, recovery $62.8{\pm}15.6s$) for 2-phenoxyethanol, 300 ppm (induction $127.3{\pm}13.3s$, recovery $107.5{\pm}4.8s$) for lidocaine-HCl and 200/100 ppm (induction $81.2{\pm}17.2s$, recovery $98.3{\pm}19.7s$) for lidocaine-$HCl/NaHCO_3$. Thus, 200/100 ppm of lidocaine-$HCl/NaHCO_3$ was found to be an effective anesthetic agent.

Comparative Efficacy of Clove oil, MS-222 and 2-Phenoxyethanol as Anesthetics in Juvenile Scomber japonicus (고등어(Scomber japonicus) 치어의 마취제로서 Clove oil, MS-222 및 2-Phenoxyethanol의 평가)

  • Han, Seock-Jung;Kim, Kyong-Min;Choi, Nack-Jung;Koo, Jun-Ho;Park, Chung-Kug;Lee, Won-Gyo;Ji, Seung-Chul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.653-657
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    • 2011
  • The efficiency of clove oil, MS-222, and 2-phenoxyethanol was evaluated as anesthetics in juvenile Scomber japonicus. Stage A5 of anesthesia was assumed to be sufficient for conducting routine aquaculture procedures in less than 3 min, with recovery (stage R5) in less than 5 min. The lowest effective doses of the three anesthetics were 50 mg $L^{-1}$ clove oil (anesthetic time of 71.3 s and recovery time of 167.0 s), 100 mg $L^{-1}$ MS-222 (anesthetic time of 70.7 s and recovery time of 115.7 s), and 400 mg $L^{-1}$ 2-phenoxyethanol (anesthetic time of 86.7 s and recovery time of 95.0 s). Anesthetic times decreased with increasing doses for all three anesthetic agents, and fish anesthetized with clove oil exhibited the longest recovery times. After 30 min, the highest plasma cortisol and lactate levels were detected with the use of clove oil, whereas the lowest values were observed with 2-phenoxyethanol. In addition, high glucose levels were maintained during recovery with clove oil, but the treatments did not significantly differ. The most effective of the three anesthetic agents was 2-phenoxyethanol, although all were considered acceptable for use in cultures of juvenile Scomber japonicus.