• Title/Summary/Keyword: Aquacultural waste

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Effects of Inoculated Bacillus subtilis on Geosmin and 2-Methylisoborneol Removal in Suspended Growth Reactors Using Aquacultural Waste for Biofloc Production

  • Luo, Guozhi;Wang, Jiao;Ma, Niannian;Liu, Zefeng;Tan, Hongxin
    • Journal of Microbiology and Biotechnology
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    • v.26 no.8
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    • pp.1420-1427
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    • 2016
  • Geosmin and 2-methylisoborneol (2-MIB) are two of the most common taint compounds that adversely affect the quality of aquacultural animals. In the present study, 94% of geosmin and 97% of 2-MIB in suspended growth reactors producing bioflocs (SGRs) with aquaculture waste were removed after inoculation with Bacillus subtilis, significantly higher than that of control SGRs (70% of geosmin and 86.4% of 2-MIB). The lowest concentrations of geosmin and 2-MIB achieved in the effluent of the SGRs were 2.43 ± 0.42 ng/l and 2.23 ± 0.15 ng/l, respectively. The crude protein content of the bioflocs produced in the SGRs was 35 ± 4%. The NH4+-N and NO2--N concentrations in the effluent of the reactors were 1.13 ± 0.21 mg/l and 0.42 ± 0.04 mg/l, respectively. These results suggest that inoculated with Bacillus subtilis, SGRs have a better performance to reuse the nitrogen in fish waste and to remove geosmin and 2-MIB from the culture water efficiently.

Effect of Air Distributor Pore Size in Foam Separator of Sea Water (해수의 포말분리시 공기분산기 기공크기 영향)

  • SUH Kuen-Hack;KIM Byong-Jin;LEE Jung-Hoon;LIM Jun-Heok;YI Gyeongbeom;KIM Yong-Ha;JO Jae Yoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.3
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    • pp.254-262
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    • 2003
  • Effect of the air distributor pore size for the removal of aquacultural waste, such as protein, total suspended solids (TSS), chemical oxygen demand (COD), turbidity and total ammonia nitrogen (TAN) from sea water was investigated by using foam separator. With the increase of pore size of air distributor, removal rates and efficiency of protein decreased. Removal rate by commercial air stone was in the range between the removal rates by G2 and G4 sintered glass discs. Within the range of pore size distributor from Gl to G4, the removal efficiency of protein were ranged from 21 to $42\%.$ The changes of removal rates and efficiencies of TSS, COD and turbidity were similar to proteins. TAN was removed by stripping. The pore size of air distributor for a higher overall oxygen mass transfer coefficient and saturation efficiency provided the condition for higher protein removal rate. Also the foam separator could be used as an aerator.

Isolation and Characterization of Ammonia Oxidizing Bacteria, Nitrosomonas sp. PK1 (암모니아 산화 세균 Nitrosomonas sp. PK1의 분리 및 특성)

  • Kim, Dae-Kyung;Kim, Hyun-Kuk;Kim, Jong-Soek;Suh, Kuen-Hack;Kim, Sung-Koo;Kong, In-Soo
    • Journal of Life Science
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    • v.7 no.2
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    • pp.107-111
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    • 1997
  • To remove dissolved $NH_{4}$$^{+}$ in the aquaculture system, one ammonia oxidizing bacterium, Nitrsomonas sp. PK1, was isolated from samples collected in many aquacultural place and sludges of waste water. The stationary phase of this atrain was reached after 9 days, and the maximum $NO_{2}$ production was shown from 3 days to 9 days. In the selective medium, 0.1% of glucose was the good carbon source for growth. However, the $NO_{2}$productivity was repressed by the addition of glucose to the medium. When $Zn^{++}$ ion was supplemented to the medium, growth and the $NO_{2}$ productivity was increased, 10mM of $ZnCl_{2}$ was the optimal concentration for growth and 1 mM of $ZnCl_{2}$ was the optimal concentration for the production of $NO_{2}$, respectively.

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Isolation of Ammonia Oxdizing Bacteria and their Characteristics (암모니아 산화세균의 분리와 그 특성)

  • LEE Myung Suk;PARK Jee Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.5
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    • pp.760-766
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    • 1998
  • This study was carried out far isolation and characterization of ammonia oxidizing bacteria (AOB) from aquacultural place and sludges of waste water collected in Pusan. One autotrophic AOB, Nitrosomonas sp. and 8 heterotrophic AOB (2 strains of Bacillus sp., 2 strains of Acinetobacter sp., Xanthomonas sp., Alcaligenes sp., Pseudomonas sp., Sphingobacterium sp.) were isolated. and identified. Variation of total nmmonia nitrogen (TAN) and $NO_2-N$ in mineral salt media containing 10mg/ $\ell$ of NHCl for 15 days in differents 9 strains was measured in order to examine the ablitity of ammonia oxidation. TAN was started to reduce after 4 days incubation and ca. 2 mg/$\ell$ of TAN was decreased after 15 days incubation by Nitrosomonas sp., At that time, $NO_2-N$ was produced to 0.023$\~$0.036 mg/$\ell$. Heterotrophic AOB showed the low ability of ammonia oxidation, 0.02$\~$0,04 mg/$\ell$ of TAN was decreased and $NO_2-N$ was produced to 0.01$\~$0.51 mg/$\ell$ after 15 days. When each strain of 8 heterotrophs was incubated in mimeral salt media containing 10 mg/$\ell$ $NH_4Cl$ and 50 mg/$\ell$ glucose, and 50 mg/$\ell$ $NH_4Cl$ and 5 g/$\ell$ glucose, the diminution of TAN was 1.12$\~$3.38 mg/$\ell$ and 1$\~$20 mg/$\ell$, respectively.

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