• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.49-57
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• 2016

We isolated and confirmed two S-RNases, denoted as mpS1 and mpS2, from apple rootstock 'Marubakaido' (Malus prunifolia Borkh. Var. ringo Asami). These S-RNases contained and conserved five cysteine residues and two histidine residues, which are essential for RNase activity. The mpS1 showed high similarity to S5 (99.1%) of Malus spectabilis, whereas the mpS2 showed 99.5% nucleotide sequence similarity to S26 of (Malus ${\times}$ domestica) and 99.6% to S35 of (Malus sieversii) when compared with reported S-RNases. In amino acid sequences, the mpS1-RNase was almost similar to the S5-RNase of Malus spectabilis, and the mpS2-RNase was similar to the S35 of Malus sieversii, with only one bp being different from the S26-RNase of Malus ${\times}$ domestica. The 57 S-RNases of Malus species were renamed and rearranged containing the new S-RNases, as mprpS35 (mpS2) and mprpS57 (mpS1), for determining S-genotypes and identifying new alleles from apple species (Malus spp.).

• Horticultural Science & Technology
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• v.17 no.2
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• pp.127-130
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• 1999

This study was attempted to develop the algorithm for recognizing fruits acquired from apple tree images with digital camera in sunlight. As the result of L*a*b* color transformation for reducing the effect of sunlight, a* and b* color index were effective to extract apple pixels in tree images and linear discrimination functions with color index a* b* were developed. To recognize fruits from apple pixels, those were classified into 4 patterns according to clustering condition and morphologically filtered. Test results showed that apple fruits unoccluded were exactly recognized, whereas apple fruits occluded with leaves and trunk were miscounted 2 apples on average.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.561-570
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• 2022

This study investigated the changes in planting environment and growing conditions of 'Arisoo' apple trees under shading net treatments. Apple tree canopies were fully covered with Blue (blue color net, mesh size: 4 mm × 5 mm), Black-A (black color net, mesh size: 4 mm × 5 mm), Black-B (black color net, mesh size: 5 mm × 6 mm), and uncovered trees were used as the control. Inside the tree canopies, the illuminance was observed as 100% (85.0 Klux) in the control, but it decreased to 75% (64.3 Klux) in the Blue, 73% (63.0 Klux) in the Black-A, and 77% (65.9 Klux) in the Black-B nets, respectively. The solar irradiance was observed as 755 w·m^-3 in the control, but it was decreased to 715 w·m^-3 in the Blue, 624 w·m^-3 in the Black-A, and 647 w·m^-3 in the Black-B nets, respectively. The average daily temperature was observed to be the highest in the Black-A net (31.8℃) while it was observed to be around 30.0℃ in the control, Blue, and Black-B nets. In addition, the average fruit surface temperature was observed to be the highest (39.4℃) in the control, but it was decreased to 38.1℃ in the Blue, 37.3℃ in the Black-A, and 39.0℃ in the Black-B nets. However, the average soil temperature, the shoot growth rate, and leaf SPAD values were not different in all the treatments. Fruit sunburn disorders were the highest in the control while it was observed to be lower in the fruits from trees covered with the shading nets. However, at harvest, the fruit quality parameters were not different in all the treatments. Overall, this study suggests that the apple trees covered with the shading nets reduced the illuminance, solar irradiance, fruit surface temperature, and fruit sunburn disorders. However, the shading nets did not affect tree growth and apple fruit quality.

• Mycobiology
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• v.28 no.3
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• pp.153-157
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• 2000

In this paper, the distribution, host restriction, and taxonomic position of Valsa mali are discussed.

• Journal of Korean Academy of Dental Administration
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• v.9 no.1
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• pp.61-64
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• 2021

• Mycobiology
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• v.35 no.1
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• pp.6-10
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• 2007

Present experiments were conducted to determine the possibility of artificial culture with various sawdust of P. gilvus. The pH value was 6.0 of oak sawdust, 6.5 of mulberry sawdust, 6.6 of elm sawdust, 6.3 of acacia sawdust and 6.1 of apple tree sawdust. Mycelial density on elm sawdust and acacia sawdust were lower than those of oak sawdust, and apple sawdust. Weight of fresh fruiting body showed that 179 g on oak tree, 227 g on oak sawdust, 21 g on elm tree, 76 g on elm sawdust, 106 g on apple tree, and 170 g on apple sawdust. Among them, the yield of oak substrates was the highest whereas acacia sawdust was the lowest, and it is concluded that the yields of sawdust substrates were higher than log substrates. P. gilvus grown on various sawdusts and logs used in this study have shown similar in anti-tumor activity against P388.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.222-229
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• 2022

Apples are the most grown fruit crops in the fruit industry of Korea. However, virus or viroid infection such as apple mosaic virus (ApMV), apple stem grooving capillovirus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV), apple scar skin viroid (ASSVd) causes fruit yield reduction and poor fruit quality. Therefore, in this study, we examined to established an efficient virus-free system to eliminate the most infected ASGV virus in domestic apple orchard. We investigated that the shoot growth rate and the virus removal rate in ASGV infected potted apples that were treated with heat treatment in a growth chamber (constant temperature/humidity device) maintained at 36℃, 38℃ and 40℃ for 4 weeks. Here we found that the shoot growth rate was the highest in the heat treatment group (36℃) and the virus was removed in the middle and top of the shoot but not in the bottom. The virus was did not removed in the 38℃ and 40℃ heat treatment group in all section of shoots, and the heat treatment group (40℃) died after 4 weeks of heat treatment without growth of shoots. We performed in vivo shoot-tip grafting using the shoot-tip of potted apple heat-treated at 36 ℃, and we also investigated the viability and virus removal rate, which showed 94% viability and 20% virus removal rate. Collectively, our results suggest that it would be possible to produce the virus-free apple plants through heat treatment and shoot-tip grafting.

• Journal of Bio-Environment Control
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• v.17 no.4
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• pp.306-311
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• 2008

Proper tree vigor and crop load were determined for 'Fuji'/M.9 apple trees in high density planting system from 2001 to 2003. Leaf/fruit ratio was highly correlated to mean fruit weight (y=1.715x+205.02, $R^2=0.66^{**}$) and yield (y=-35.l56x+5963.7, $R^2=0.44^{**}$). In addition, there was a significant correlation between the number of leaves per tree and mean fruit weight. However, crop load did not affect tree growth, soluble solids content of fruit, and Hunter a value. To harvest the fruits heavier than 300 g without biennial bearing, it was appropriate to crop 55 to 64 fruits in a tree with 55 leaves per fruit of adult tree. The good indices for proper tree vigor could be 20 to 25 cm of mean shoot length and above 95% of shoot termination rate. Moreover, no secondary growth and 20 to 30% of spur formation could be the indices for highly productive tree vigor.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.421-430
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• 2022

Crop load management in apple trees is important for achieving optimum productivity and crop value. Hence, we investigated the influence of different crop loads on the fruit quality, mineral content, and yield of the 'RubyS' apple variety. After 4 weeks of full bloom, the crop load was adjusted by hand thinning to different (5, 10, and 15 fruits·cm^-2) trunk cross-sectional areas (TCSA), representing low, medium, and high crop loads. The low crop load increased the fruit size and weight, the development of the red-blushed area, and the peel color a^* at harvest; however, it reduced the total number of fruits·tree^-1 and yield compared with that of the other crop loads. The medium crop load improved the fruit weight, flesh firmness, and soluble solids content and reduced the fruits·tree^-1 but did not affect the fruit size and yield. However, there were no significant differences in the titratable acidity and starch index among the crop loads. The fruit mineral content (phosphorus and potassium) was higher in the low and medium crop loads compared to the high crop load. However, the nitrogen, calcium, and magnesium contents in the fruits were not affected by the crop loads. Overall, this study suggests that a low crop load improves the fruit size and weight, but its effect on the quality and fruit mineral content is similar to that of a medium crop load. Therefore, the optimum crop load level for the 'RubyS' apple trees was approximately 10 fruits·cm^-2 TCSA.

• Korean Journal of Organic Agriculture
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• v.5 no.1
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• pp.137-147
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• 1996

For the purpose of acquiring microbial agents that can be utilized to biologically control the major airborne diseases to apple trees, such as canker(Botryosphaeria dothidea), bitter rot(Glomerella cingulata), alternaria leaf spot(Alternaria mali), root rot(rosellinia necatrix), canker(Valsa ceratosperma) and gray mold rot(Botrytis cinerea), the effective microorgaisms were isolated, tested for antagonistic activity to the pathogens causing major diseases to apple trees and identifed. Screening of more than 5,000 species of microorganisms collected in nature for them antagonistic action to the pathogens causing 5 major diseases to apple trees resulted in selection of effective species. Out of the 11 species, one species designated as CAP134 demonstrated outstanding activity. The bacterial strain, CAP134 exerted antagonistic efficiency of 57% on an isolated strain and 40% on a donated strain of Botryosphaeria dothidea., 52% on an isolated strain and 46% on a purchased strain of Alternaria mali, 60% on Valsa ceratosperma 25% on Glomerella cingulata, and 64% Rosellinia necatrix. The CAP134 was identified as a bacterial strain to Bacillus subtilis ATCC 6633 based on morephology, culture conditions, and physio-biochemical characteristics.