• 대한지구과학교육학회지
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• 제13권1호
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• pp.29-39
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• 2020

본 연구에서는 지구 자전과 태양의 겉보기 운동을 학습하기 위한 역할놀이 활동의 학습 효과 및 어려움을 알아보기 위해서 역할 놀이 활동을 통한 개념 이해정도, 면담으로 수집한 활동에서 나타나는 어려움, 활동 중 시선이동을 분석하였다. 연구 참여자는 초등학교 5학년 학생 22명이며, 역할 놀이 중 실험 행동과 사후 면담, 시선이동자료를 수집하여 분석하였다. 연구결과, 학생들은 역할 놀이 활동을 통해 지구의 자전과 자전방향은 설명할 수 있지만 태양의 겉보기 운동을 설명하는 것은 어려워했다. 학생들은 역할 놀이 활동을 통해 태양의 겉보기 운동을 지각하기 어려워하기 때문에, 자전에 의해 나타나는 태양의 겉보기 운동 개념을 학습하기 위한 역할 놀이 활동은 학습 효과가 낮다.

• 펄프종이기술
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• 제31권3호
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• pp.1-9
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• 1999

Effect of furnish on enzyme activity was investigated by using the three components (cellulose, enzyme, and cationic polyelectrolyte) model papermaking system. Avicel was used as a cellulose model compound to observe the effect of adsorption and desorption of enzyme with other component and the resultant change of particle size. As an experimental result, the enzyme loses considerably its apparent activity due to the adsorption onto cellulose and cationic polyelectrolyte. Activities of enzyme applied to the actual papermaking stocks having controlled fiber length showed different behavior in terms of pulp species UKP and KOCC stocks. That is, the enzyme activity in UKP was increased as fines content increased, however, vice versa in KOCC stock . This result can be considered to be the existence of various contaminants included in the fines of KOCC . The effect of possible contaminants such as inorganic materials, calcium ion, surfactant, and conductivity on enzyme activity were also investigated.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.672-674
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• 1999

Cepacidine A was first identified as a novel antifungal antibiotic which was isolated from the culture broth of Pseudomonas cepacia AF200l. It showed a potent in vitro antifungal activity against various pathogenic fungi, but did not show any activity against bacteria. Recently, the immunosuppressive action of cepacidine A was discovered using an in vitro screening system involving inhibition of the proliferation of murine lymphocytes stimulated by 2 mitogens, and also by in vivo mouse models involving inhibition of delayed type hypersensitivity and SRBC hemagglutination. Cepacidine A showed a significant activity of cellular immunosuppression (ED/sub 50/) at concentration levels of 1-3 ㎎/㎏, i.p.. Unfortunately, the delayed toxicity at a dose of above 3 ㎎/㎏ i.p. was apparent.

• Archives of Pharmacal Research
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• 제24권1호
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• pp.55-63
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• 2001

Aucubin, an irdoid g1ucoside, was investigated to determine whether it has a stimulating effect on $\alpha$-amanitin excretion in $\alpha$-amanitin intoxicated rats, and whether there is binding activity to calf thymus DNA. High-performance liquid chromatography (HPLC) analysis of $\alpha$-amanitin in rat urine allowed quantitative measurement of the $\alpha$-amanitin concentration with a detection limit of 50${mu}g/ml$. In this system, a group treated with both $\alpha$-amanitin and aucubin showed that o(-amanitin was excreted about 1.4 times faster than in the $\alpha$-amanitin only treated group. Our previous results showed that the toxicity of $\alpha$-amanitin is due to specific inhibition of RNA polymerase activity and the resultant blockage of the synthesis of certain RNA species in the nucleus. However, no significant activity change on RNA polymerase from Hep G2 cells was observed when aucubin was treated with $\alpha$-amanitin at any concentration tested. Nevertheless, aucubigenin inhibited both DNA polymerase (IC50, 80.5${mu}g/ml$) and RNA polymerase (IC50, 135.0${mu}g/ml$) from the Hep G2 cells. The potential of both $\alpha$-amanitin and aucubin to interact with DNA were examined by spectrophotometric analysis. $\alpha$-Amanitin showed no significant binding capacity to calf thymus DNA, but aucubin was found to interact with DNA, and the apparent binding constant ($K_{app}$) and apparent number of binding sites per D7A phosphate ($B_{app}$) were 0.45$0.45{\times}$$10^4$ $M^{-1}$ and 1.25, respectively.

• 한국식품과학회지
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• 제26권4호
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• pp.403-410
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• 1994

국내에서 생산되고 있는 공장산 고추장의 제조후 경과기간에 따른 품질특성치의 변화를 구명하기 위하여 시제된 공장산 고추장을 $37^{\circ}C$에서 105일간 저장하면서 고추장의 품질변화를 측정한 결과는 다음과 같았다. 환원당은 저장 15일까지는 비교적 큰폭으로 감소한 다음 완만하게 감소하였다. 또한 유리당으로는 glucose, fructose, maltose가 검출되었으며 이중에서는 glucose가 가장 많았다. 유리아미노산 함량은 저장기간이 길어짐에 따라 감소하는 경향이었다. 즉, 저장초기 유리아미노산 총량은 2092.50mg%에서 저장 60일후 22.3% 정도 감소하였으며 90일 후에는 1360.57mg%로 저장초기에 비해 35% 정도가 감소한 것으로 나타났다. 저장기간중 전반적으로 효소활성은 유의적인 변화를 보이지 않았으나, 증성 protease의 활성이 미약하나마 완만하게 증가되는 경향을 보였다. 수분함량 및 수분활성도는 각각 저장기간의 증가에 비례하여 감소하는 추세를 보였으며, 양자간의 상관계수는 r=0.964였다. 저장기간의 경과에 따른 겉보기점도는 증가하였으며, 수분함량보다는 수분 활성도에 의해 더 큰 영향을 받는 것으로 판단되었다.

• BMB Reports
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• 제28권5호
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• pp.458-463
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• 1995

Two apparent rho-independent transcription terminator structures within the coding sequence of aceK have been destroyed to access their roles in the differential expression between aceA and aceK in the glyoxylate bypass operon of E. coli. The effect of mutations on the expression of aceK was evaluated in two different ways: one by maxicell labeling and the other by lacZ fusion gene construction. The maxicell labeling experiment with the mutant operon clones has failed, like that of the wild type operon clone, to visibly show isocitrate dehrogenase (IDH) kinase/phosphatase, the product of aceK, on the autoradiogram of a protein gel. When the same mutations were introduced into an aceK::lacZ fusion gene to quantitatively evaluate the mutational effect, the activity of ${\beta}-galactosidase$ in neither of the mutant versions of the fusion gene was elevated significantly enough to explain the degree of polarity observed in this region. Thus, we conclude that neither of these intragenic, apparent rho-independent transcription terminator structures, which have long been suspected as a major determinant in the down regulation of aceK, really act as a premature transcriptional terminator.

• 천문학논총
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• 제30권2호
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• pp.429-432
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• 2015

We present a kinematic study of the parsec-scale radio jet in OJ 287, one of the most studied BL Lac objects, during ${\gamma}$-ray flares, to explore the relation between parsec-scale radio jet activity and ${\gamma}$-ray emission. The 22-GHz light curve of OJ 287 show three obvious flare events around 2011 May, 2011 October, and 2012 March. The second radio flare occurred during the ${\gamma}$-ray flaring period, and the third radio flare seemed to precede the ${\gamma}$-ray flare by one month. One jet component moved outward with respect to the core component with an apparent superluminal speed (~ 11c) from 2010 November to 2011 November. Then it changed direction, moving apparently inward in 2011 November, when the ${\gamma}$-ray flare occurred. The observed apparent inward motion of the jet at 22 GHz could be caused by a new jet component, unresolved at 22 GHz, in the innermost region.

• 생명과학회지
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• 제27권8호
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• pp.930-936
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• 2017

Pseudomonas sp. GP32가 생산하는 생물고분자응집제 Biopol32의 실제 산업폐수에서의 적용을 위하여 생물고분자 Biopol32의 응집특성을 조사하였다. Biopol32의 응집물질은 polysaccharide로 확인되었다. 음이온성 응집제들은 응집효율을 높이기 위하여 보조응집제로 counter ion을 사용하고 있다. Biopol32의 응집활성은 보조응집제로 $Ca^{2+}$, $Al^{3+}$와 같은 양이온을 첨가 하였을 때 크게 증가하였으며, 보조응집제로서 7.0 mM $CaCl_2{\cdot}2H_2O$을 첨가하였을 때 Biopol32의 응집활성이 가장 높게 나타났다. Kaolin 현탁액에 Biopol32를 1.5 mg/l의 농도로 첨가하였을 때 가장 높은 응집활성을 보였다. Biopol32의 pH와 온도에 따른 응집활성은 현재 폐수처리 현장에서 상업적으로 이용되고 있는 음이온성 유기합성고분자응집제 polyacrylamide와 Zoogloea ramigera로부터 생산된 생물고분자응집제 zooglan과 응집활성을 비교하였다. Biopol32의 응집활성은 pH 5.0에서 8.0의 넓은 범위의 pH에서 높은 응집활성을 보였으며, 또한 온도의 영향에서는 $60^{\circ}C$에서 가장 높은 응집활성을 나타내었으나, $70^{\circ}C$ 이상의 온도에서는 응집활성이 급격히 감소하는 것으로 나타났다. Jar fermentor를 이용한 batch culture를 통하여 Biopol32의 응집활성과 전하밀도와의 관계를 조사하였다. 음이온성 전하밀도와 겉보기 점도가 높을수록 Biopol32의 응집활성이 높아져, Biopol32의 응집활성과 겉보기점도는 Biopol32의 전하밀도와 밀접한 관계가 있는 것으로 나타났다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.554-557
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• 2000

Strain JV3 was isolated from commercial Swiss cheese products and identified as a bacteriocin producer, which has bactericidal activity against Leuconostoc mesenteroides KCCM 11324. Strain JW3 was identified tentatively as Lactococcus lactis by the API test. The activity of lacticin JW3, named tentatively as the bacteriocin produced by Lactococcus lactis JW3, was detected during the mid-log growth phase, and reached a maximum during the early stationary phase, and decreased after the late stationary phase. Its antimicrobial activity on sensitive indicator cells was completely disappeared by protease IV. The inhibitory activities of lacticin JW3 were detected during treatments of up to $121\'^{circ}C$ for 15 min. Lacticin JW3 was very stable over a pH range of 2.0 to 9.0 The apparent molecular mass of lacticin JW3 was estimated to be in the region of 3-3.5kDa, which was determined by the direct detection of bactericidal activity after SDS-PAGE.

• BMB Reports
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• 제37권6호
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• pp.753-756
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• 2004

Membranes prepared from Bacillus cereus KCTC 3674, grown aerobically on a complex medium, oxidized NADH exclusively, whereas deamino-NADH was little oxidized. The respiratory chain-linked NADH oxidase exhibited an apparent $K_m$ value of approximately $65\;{\mu}m$ for NADH. The maximum activity of the NADH oxidase was obtained at about pH 8.5 in the presence of 0.1 M KCl (or NaCl). Respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO) inhibited the activity of the NADH oxidase by about 90% at a concentration of $40\;{\mu}m$. Interestingly, rotenone and capsaicin inhibited the activity of the NADH oxidase by about 60% at a concentration of $40\;{\mu}m$ and the activity was also highly sensitive to $Ag^+$.