• 한국자기공명학회:학술대회논문집
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• 한국자기공명학회 2002년도 International Symposium on Magnetic Resonance
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• pp.74-74
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• 2002

• 동의생리병리학회지
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• 제18권4호
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• pp.1192-1198
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• 2004

Sasang Constitutional Medicine is a major branch of Korean Traditional Medicine. The differences of disease susceptibility to be shown in Sasang constitution may be due to genetic factors. Therefore, I examined interrelationship among cerebral infarction (CI), apolipoprotein E (apo E) gene polymorphism, and Sasang constitutional classification. Apo E is a key protein modulating the highly atherogenic apoB containing lipoproteins and is a candidate gene for the development of coronary artery disease (CAD). The ε2 and/or ε4 alleles were the first to be implicated in premature CAD, which resulted in this polymorphism being extensively studied. I investigated the association between apo E genotype and CI by case-control study in a Korean population. I also classified CI patients and control group into groups according to Sasang Constitutional Medicine. 218 CI patients and 379 controls without CI were examined. Apo E genotype was determined by 8% polyacrylamide gel separation after DNA amplification. A frequency of apo E ε3/ε3 in the apo E genotype distribution was higher in the CI patients compared with that in controls. Also, it was widely known that Taeumin was easily attacked with CI, but there was no association between apo E polymorphim and Taeumin. However, the Taeumin constitution did not enhance the relative risk for CI in the subjects with apo E ε2 and/or ε4 alleles. No differences in the apo E genotypes frequencies were observed in the Taeumin compared with that in the other constitutions. In addition, I investigated whether the DD(deletion/deletion) or ID(insertion/deletion) genotype of angiotensin converting enzyme (ACE) gene, a candidate gene for CI, was associated with CI, Taeumin constitution, and apo E polymorphism. As a result, the frequency of Taeumin constitution was significantly higher in CI patients with both apo E ε3/ε4 and ACE ID/DD genotypes than in the remaining Sasang constitutions. In summary, it was concluded that the apo E polymorphism is a major risk factor for CI in Koreans and the ACE ID/DD genotype enhanced the relative risk for CI in the subjects with apo E ε3/ε4 genotype and Taeumin constitution.

• 생명과학회지
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• 제16권7호
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• pp.1071-1079
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• 2006

야생 돌복숭아(Prunus persica Batsch var. davidiana Max.)의 생리활성 추출물질이 선천성 고혈압 흰쥐(SHR)의 지질대사 이상 및 고혈압 예방과 개선효과 등에 생리생화학적 효능이 있을 것으로 추정되어 Wistar 계 수컷 SHR을 사용하여, 기본식이와 물만을 급여한 대조군인 Control군과 SHR에 돌복숭아 5.0g% 추출액을 급여한 군(5g% Ex.군) 및 SHR에 돌복숭아 10.0g% 추출액을 섭취시킨 10g% Ex.군을 33일간 실험 사육하여 혈청 지질성분 및 혈압 저하 효과를 생리적 측면에서 검토하기 위하여 본 실험을 수행하였다. 혈청 중의 총 콜레스테롤 농도, 중성지방, LDL-콜레스테롤, 유리 콜레스테롤 및 동맥경화지수 등에서 돌복숭아 생리활성물질 5.0g%, 10.0g% 추출액을 섭취시킴으로서 유의적인 농도 등의 감소 효과를 보였으며, HDL-콜레스테롤 농도 및 총 콜레스테롤에 대한 HDL-콜레스테롤 비는 상승되는 것으로 나타났다. 한편, 간장 및 뇌 중의 총 콜레스테롤, 중성지방 농도는 돌복숭아 5.0g%, 10.0g% 추출액을 섭취시킨 군에서 감소되는 것으로 나타났다. 반면, 혈청 apolipoprotein(Apo) A-I, Apo A-II 농도 등은 돌복숭아 5.0g%, 10.0g% 추출액을 섭취시킨 군에서 유의적으로 증가되는 것으로 나타났다. 또한 Apo C-II, Apo C-III, Apo E 및 Apo A-I에 대한 Apo B의 농도 비는 감소되었으며, 혈압의 변화는 SHR에 돌복숭아 5.0g% 및 10.0g% 추출액을 섭취시킴으로서 혈압이 저하됨을 관찰할 수가 있었다. 돌복숭아 5.0g% 농도(5g% Ex.군)와 10.0g% 농도(10g% Ex.군)의 추출액 섭취에 따른 두 군간에 각종 지질성분과 apolipoprotein 분획 농도 및 혈압 변화의 비교는 유의적인 차이는 없었다 따라서 야생 돌복숭아 중의 생리활성물질 추출액 섭취가 선천성 고혈압 흰쥐에 대한 혈청 지질개선 및 심장순환기계 질환, 고혈압의 예방과 치료개선에 효과가 있을 것으로 생각된다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 1994년도 춘계심포지움 및 발표논문 초록
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• pp.38-38
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• 1994

• BMB Reports
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• 제53권5호
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• pp.284-289
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• 2020

Tamoxifen, a nonsteroidal estrogen receptor (ER) antagonist, is used routinely as a chemotherapeutic agent for ER-positive breast cancer. However, it is also causes side effects, including retinotoxicity. The retinal pigment epithelium (RPE) has been recognized as the primary target of tamoxifen-induced retinotoxicity. The RPE plays an essential physiological role in the normal functioning of the retina. Nonetheless, potential therapeutic agents to prevent tamoxifen-induced retinotoxicity in breast cancer patients have not been investigated. Here, we evaluated the action mechanisms of sulfasalazine against tamoxifen-induced RPE cell death. Tamoxifen induced reactive oxygen species (ROS)-mediated autophagic cell death and caspase-1-mediated pyroptosis in RPE cells. However, sulfasalazine reduced tamoxifen-induced total ROS and ROS-mediated autophagic RPE cell death. Also, mRNA levels of tamoxifen-induced pyroptosis-related genes, IL-1β, NLRP3, and procaspase-1, also decreased in the presence of sulfasalazine in RPE cells. Additionally, the mRNA levels of tamoxifen-induced AMD-related genes, such as complement factor I (CFI), complement factor H (CFH), apolipoprotein E (APOE), apolipoprotein J (APOJ), toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4), were downregulated in RPE cells. Together, these data provide novel insight into the therapeutic effects of sulfasalazine against tamoxifen-induced RPE cell death.

• 한국산학기술학회논문지
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• 제18권2호
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• pp.549-558
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• 2017

본 연구는 체지방률이 32% 이상인 폐경 후 비만여성 36명을 대상으로 유산소운동이 혈청지질, 렙틴, 그렐린 및 인슐린저항성지수에 미치는 영향을 구명하기 위하여 운동집단(18명)과 통제집단(18명)으로 나누어 유산소운동인 라인댄스를 16주간 실시한 후 체조성, 혈청지질, 렙틴, 그렐린 및 인슐린저항성지수를 운동전과 후로 측정하여 비교분석하였다. 본 연구의 결과 운동 후에 운동집단에서 체중, 체지방률(%BF), 체질량지수(BMI), 내장지방면적(VFA), 렙틴(Leptin), 인슐린(Insulin), 글루코스(Glucose), 인슐린저항성지수(HOMA-IR), 총콜레스테롤(TC), 중성지방(TG), 아포리포프로틴(Apolipoprotein) B, 저밀도지단백 콜레스테롤(LDL-C) 및 수축기혈압(SBP)이 유의하게 감소하였다. 또한 아포리포프로틴(Apolipoprotein) A-1, 그렐린(Ghrelin) 및 고밀도지단백 콜레스테롤(HDL-C)이 유의하게 증가하였다. 유산소운동집단의 내장지방면적에 영향을 미치는 에너지대사조절인자의 회귀분석결과에서 주요요인으로 그렐린, 인슐린, 글루코스 및 HOMA-IR 나타났다. 따라서 규칙적이고 지속적인 유산소운동이 폐경 후 비만여성의 체조성, 내장지방, 혈청지질, 렙틴, 그렐린 및 HOMA-IR을 개선시키는 데 효과가 있을 것으로 생각된다.

• 한국산학기술학회논문지
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• 제21권1호
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• pp.477-486
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• 2020

본 연구는 알츠하이머 치매노인을 대상으로 Apolipoprotein E (ApoE) ɛ4의 유무와 치매수준에 따른 우울과 기억력의 상관관계를 분석하고자 하였다. 임상치매척도(clinical dementia rating; CDR)가 0.5점에서 2점 사이인 65세 이상 노인 50명을 대상으로 임상치매척도, 노인용 서울언어학습검사, 레이 복합도형검사, 축약형 노인우울검사를 실시하였고 ApoE 유전자형은 구강상피세포를 채취하여 실시한 유전자 검사를 통해 확인하였다. 자료분석은 맨 휘트니 U 검정, 상관관계 분석을 실시하였다. ApoE ɛ4가 없는 경우에 CDR 1점과 2점에서 우울과 언어적 즉시회상 기억력이 유의한 음의 상관관계가 있었고(p<.05), ApoE ɛ4를 보유한 경우에는 CDR 1점에서 우울과 언어적 즉시회상, 언어적 지연회상 기억력에서 유의한 음의 상관관계가 있었다(p<.05). 우울과 언어적 즉시회상 기억력은 유의한 상관관계가 있었고 ApoE ɛ4를 보유한 경우에는 우울과 언어적 지연회상 기억력에도 유의한 상관관계가 있었다. 따라서 알츠하이머 치매의 예방 및 중재로 시각적인 훈련보다 언어적 기억력훈련이 유용할 것이며 우울치료가 상호보완적으로 도움을 줄 수 있을 것으로 기대한다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권12호
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• pp.1780-1785
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• 2005

Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC). The fraction collected was then confirmed as VLDL using 4% polyacrylamide gel electrophoresis and transmission electron microscopy (TEM). The particle size of VLDL is 46.8${\pm}$8.6 nm. The VLDL fraction was then subfractionated and the apolipoprotein (apo) profile was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE). The CB and AK have almost similar types of apo in both subfractions 1 and 2. The AK showed the presence of apoAI, AIV, D and E whereas the CB had apo AIV, D, E and H. The apo AIV and apo E were present in both subfractions of AK and CB.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1080-1088
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• 2013

Our previous results showed that kisspeptin-10 (Kp-10) injections via intraperitoneal (i.p.) once daily for three weeks notably promoted the egg laying rate in quails. In order to investigate the mechanism behind the effects of Kp-10 on enhancing the egg laying rate in birds, this study focused on the alternations of lipids synthesis in liver after Kp-10 injections. 75 female quails (22 d of age) were allocated to three groups randomly, and subjected to 0 (control, Con), 10 nmol (low dosage, L) and 100 nmol (high dosage, H) Kp-10 injections via i.p. once daily for three weeks, respectively. At d 52, quails were sacrificed and sampled for further analyses. Serum $E_2$ concentration was increased by Kp-10 injections, and reached statistical significance in H group. Serum triglyceride (TG) concentrations were increased by 46.7% in L group and 36.8% in H group, respectively, but did not reach statistical significance, and TG contents in liver were significantly elevated by Kp-10 injections in a dose-dependent manner. Serum total cholesterol (Tch) concentrations significantly decreased in H group, while in H group the hepatic Tch content was markedly increased. The level of non-esterified fatty acid (NEFA), apolipoprotein A1 and B (apoA1 and apoB) were not altered by Kp-10 injections. The genes expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthetase (FAS), apolipoprotein VLDL-II (apoVLDL-II), cholesterol $7{\alpha}$-hydroxylase (CYP7A1) and vitellogenin II (VTG-II) were significantly up-regulated by high but not low dosage of Kp-10 injection compared to the control group. However, the expression of SREBP-2, acetyl-CoA carboxylase ($ACC_{\alpha}$), malic enzyme (ME), stearoyl-CoA (${\Delta}9$) desaturase 1 (SCD1), apolipoprotein A1 (apoA1), fatty acid binding protein 2 (FABP2), 3-hydroxyl-3-methyl glutaryl-coenzyme A reductases (HMGCR), estrogen receptor ${\alpha}$, ${\beta}$($ER{\alpha}$ and ${\beta}$) mRNA were not affected by Kp-10 treatment. In line with hepatic mRNA abundance, hepatic SREBP1 protein content was significantly higher in H group. Although the mRNA expression was not altered, the content of $ER{\alpha}$ protein in liver was also significantly increased in H group. However, SREBP-2 protein content in liver was not changed by Kp-10 treatment. In conclusion, exogenous Kp-10 consecutive injections during juvenile stage significantly advanced the tempo of egg laying in quails, which was associated with the significant elevation in hepatic lipids synthesis and transport.

• 보건의료산업학회지
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• 제7권3호
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• pp.71-82
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• 2013

Arterial stiffness(AS) is an important pathologic state of vascular injury. This study was carried out to elucidate the effect of physiological variables on brachial-ankle pulse wave velocity(BAPWV), index of AS. Four hundred adults(volunteers) participated in this study. Body indices, biochemical, cardiac and inflammatory markers, and right(Rt)- and left(Lt)-BAPWV were measured. Body mass index(BMI), Rt- and Lt-BAPWV, glucose, triglyceride, alkaline phosphatase(ALP), gamma-glutamyl transferase(GGT), creatinine, uric acid, troponin-I(TNI), NT-proBNP and high sensitivity C-reactive protein(hs-CRP) levels were higher than the reference value of each variable. Rt- and Lt-BAPWV were directly correlated with age, body weight, BMI, glucose, ketone, aspartate aminotransferase, alanine aminotransferase, ALP, GGT, total cholesterol, low density lipoprotein, lipoprotein(a), apolipoprotein-B, blood urea nitrogen, heart rate, TNI, creatine kinase, CK-MB, lactic dehydrogenase, myoglobin, hs-CRP, lipase, reumatoid factor, fibrinogen and D-dimer (P<0.05, P<0.01, P<0.001 or P<0.000, respectively), but inversely associated with total bilirubin, uric acid, apolipoprotein-A1 and GFR (P<0.05). These observations suggest that a variety of physiological variables may influence BAPWV, resulting in increased risk or prevention of cardiovascular and/or cerebrovascular attacks. Therefore, physiological variables affecting BAPWV should be regularly controlled.