• Title/Summary/Keyword: Aphid

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Development and Predation of a Aphidophagous Gall Midge, Aphidoletes aphidimyza (Roundani) (Diptera: Cecidomyiidae) on Myzus persicae Sulzer (진딧물류를 포식하는 혹파리, Aphidoletes aphidimyza(Roundani)(Diptera: Cecidomyiidae)의 복숭아혹진딧물 포식과 발육)

  • 최만영;이건휘;백채훈;김두호
    • Korean journal of applied entomology
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    • v.40 no.1
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    • pp.44-49
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    • 2001
  • Development and predation of an aphidophagous gall midge, Aphidoletes aphydimyza (Rondani) , on the green peach aphid, Myzus persicae Sulzer, were studied under laboratory and greenhouse conditions. The developmental threshold temperature of the gall midge larvae was estimated to be $13^{\circ}C$. Larval and total developmental periods were 5.3 and 29 days at 25 $\pm$$2^{\circ}C$. The gall midge attacked more aphids as increasing density of peach aphids, and consumed 4 out of 10 peach aphids per day in a petri dish (9 cm in diameter) at maximum. In green house, the gall midge could control the density of the green peach aphids efficiently with a delayed density dependent-like pattern.

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Insecticidal Effect of Neem Cake Extracts on Cabbage Pests, Aphis gossypii and Pluetella xylostella

  • Lee. HoYong;Kim, Won-Rok;Min, Bong Hee
    • Korean Journal of Environmental Biology
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    • v.22 no.4
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    • pp.501-506
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    • 2004
  • In organic agriculture, choose of effective and cheap bio-pesticide is very important. The authors developed an insecticidal extract from neem cake, waste of neem oil from kernel, and applied as a bio-pesticide. Bio-pesticide neem cake extracts experiment on cabbage pest was carried out at Wonju Agricultural Technology and Extension Center from 11 March to 30 May 2003. There were six treatments with three replications, using completely randomized design. Treatments involved three and six sprays of synthetic pyrethroid pesticide cypermethrin 10 EC at the dilution rate of 2.2 mL $L^{-1}$ of distilled water and four, five and six sprays of bio-pesticide neem at the dilution rate of 13.3 mL $L^{-1}$ of distilled water, and untreated control. For each treatment, designated sprayings were done at 7 days interval. Pre-spray data showed that the plants in all the experimental plots were already infested with aphid (Aphis gossypii), and diamondback moth (Pluetella xylostella). The results indicated that all neem pesticide treatments were more effective in insecticidal activity than the untreated control and the chemical treatments in controlling aphids and diamondback moth. Among the three neem treatments, there were no significant differences between them.

Persistence of the Enzymatic Activity of Dietary Acid Phosphatases from the Lumen of the Midgut of the Lady Beetle, Harmonia axyridis (무당벌레(Harmonia axyridis)의 중장내 먹이 Acid Phosphatase(AP)의 활성변화)

  • 홍옥기;박해철;박규태;박용철
    • Korean journal of applied entomology
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    • v.34 no.2
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    • pp.95-99
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    • 1995
  • Acid phosphatase(AP) of he aphid, Megoura crassicauda and the major component of the lady beetle's artificial diet, fresh chicken liver, was adapted as a model protein to study the digestion of diet proteins in the midgut of Harmonia axyridis. The lady beetle did not secrete its own AP into the lumen of the midgut. The aphid and the live chicken liver had AP which was still alive in enzymatic activity from the extract of the lumen of the midgut of the lady beetle. The digestive ability of the lady beetle on proteins turned out to be different depending on food sources. In the lumen of the midgut of the lady beetle, though most of AP of live chicken liver lost its activity withtin 12 hours, that of M. cassicauda kept strong enzymatic activity up to 24 hours.

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Detection of the Specific DNA-binding Proteins for the Aphid rRNA (진딧물 rRNA 유전장에 특이적으로 결합하는 단백질 탐색)

  • O-Yu Kwon;Dong-Hee Lee;Tae-Young Kwon
    • Korean journal of applied entomology
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    • v.34 no.2
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    • pp.100-105
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    • 1995
  • A whole body extract (WBE), a crude nuclear fraction, of aphids was prepared and used to identify the proteins which bound specifically to 5'-upstream regions of the transcription initiation site of the aphid ribosomal RNA gene (rDNA). While DNA fragment (-263/-195) was bound by only one specific 53 kDa protein, two DNA fragments, A(-194/23) and B(-393/-264), were commonly bound by three proteins (52 kDa, 50 kDa and 40 kDa). It was also revealed that the formation of he DNA-protein complex requires a cation.

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Rearing of a Green Lacewing, Chrysopa pallens Ramber, on Artificial Diets (인공사료를 이용한 칠성풀잠자리붙이의 사육)

  • 최만영;이종진;이건휘
    • Korean journal of applied entomology
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    • v.38 no.1
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    • pp.35-39
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    • 1999
  • An aphid predator, Chrysopa pallens Ramber, was reared on the artificial diets containing chicken egg yolk, yeast hydrolysate, brewer's yeast or Vanderzant's vitamin mixture, sucrose andlor bee honey, casein hydrolysate, and cholesterol. On these diets, 20.0 to 70.0% of the 1st instar larvae developed to apparently normal adults depending on diets used. The adults fed on one of these diets which was the most effective laid 230 fertile eggs for her 36 days of adult life span. The nonlipid part of the aphid, Myzus persicae Sulzer was thought to be nutritionally more important than the lipid part for the development of the green lacewing.

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STUDIES ON THE VIRUSES OF RADISH MOSAIC

  • KIM, Woon-Soo
    • Journal of Plant Biology
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    • v.6 no.2
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    • pp.9-21
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    • 1963
  • A mosaic diseased radish collected from the suburb of Seoul, in November, 1961 was used for studing the host range, physical properteis, purificaitiion, insect transmission, and electron microscopy. A Japanese strain of radish mosaic(RPV) was also used with Korean strain of radish mosaic (KRMV) for a comparative study. The two viruses, KRMA and RPV, were identified by the difference in host range, insect transmission and electron microscopy. The KRMA was severely infective on tobacco and Nicotiana glutinosa, while on Gomphrena globsa was immune to the virus. RPV produces necrotic local lesions on Gomphrena globosa but did not infect tobacco and N. glutinosa. Among varieties of radish, Seoul, Akamaroo, Akanagea, Koong-Joong showed more severe symptoms than Simoo, Minong, Paek-soo, which appeared to be fainly resistant. In a number of tests, it was found that the virus KRMA retained its infectivity until to a dilution of 1:2,000, heating at $58^{\circ}$ for 10 minutes, adn aging in vitro for 7 days at room temperature. The RPV was not inactivated until it was diluted to 1:2,000, heated to $56^{\circ}$, and aged for 6 days. The KRMV was readily transmitted by the aphid(Myzus persicae Sulz). The virus RPV was not transmitted by the aphid in a number of tests. Partialy purified viruses using ammonium acetate buffer, salting-out by ammonium sulfate and centrifugation of high and low speed were highly infective. Electron micrographs showed that the KRMV paticles are of spherical particles whereas the RPV particles are rod-shaped.

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