• 제목/요약/키워드: Antioxidant capacities

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Evaluation of in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius and Trianthema decandra

  • Mazumder, Upal Kanti;Gupta, Malaya;Haldar, Pallab Kanti;Kandar, Chandi Charan
    • Advances in Traditional Medicine
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    • v.7 no.3
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    • pp.290-296
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    • 2007
  • Glinus oppositifolius and Trianthema decandra belonging to the Ficoidaceae family were commonly used by tribal peoples for the treatment of liver disorders and cancer. The preliminary phytochemical screening of those plants showed the presence of flavonoids, terpenoids, tannins and saponins. The aim of this study was to evaluate the in vitro antioxidant activities of the methanol extracts of Glinus oppositifolius (MEGO) and Trianthema decandra (METD). The antioxidative capacities of MEGO and METD were determined by the following four complementary assay; DPPH radical scavenging assay, superoxide anion generation by xanthine-xanthine Oxidase assay, hydroxyl radical scavenging assay and $Fe^{2+}$-ascorbate induced by lipid peroxidation assay. The $IC_{50}$ values of the both extracts were calculated from the inhibition curve. The $IC_{50}$ MEGO and METD in DPPH, superoxide anion, hydroxyl radical scavenging and lipid peroxidation assay are 1.85, 7.31, 13.95, 22.82 and 2.21, 9.78, 14.87, 19.76 ${\mu}g/ml$ respectively. Both the extracts exhibited a significant antioxidant effects.

Evaluation of the Antioxidant Activities of Natural Components of Artemisia iwayomogi

  • Yan, Xi-Tao;Ding, Yan;Lee, Sang Hyun;Li, Wei;Sun, Ya-Nan;Yang, Seo Young;Jang, Hae Dong;Kim, Young Ho
    • Natural Product Sciences
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    • v.20 no.3
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    • pp.176-181
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    • 2014
  • The antioxidant activities of 29 components isolated from the aerial parts of Artemisia iwayomogi were evaluated in vitro and in cell culture. Among the tested compounds, 2, 6, 8, 10, 13, and 14 exhibited the greatest peroxyl radical-scavenging activities in the oxygen radical absorbance capacity (ORAC) assay, and 2, 10, and 14 also showed significant reducing capacities. However, all compounds showed weak metal chelating activities. Their cellular antioxidant activities were evaluated in HepG2 cells. At $10{\mu}M$, compounds 6, 8, and 14 exhibited stronger protection against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress than compounds 2, 10, and 13. Moreover, Compounds 2 and 8 were more effective in protecting against $Cu^{2+}$-induced oxidative stress than compounds 6, 10, 13, and 14 at $10{\mu}M$. These results suggest that the phenolic compounds in A. iwayomogi have the potential to be developed as natural antioxidants for the treatment of oxidative stress-related diseases.

Phenolic contents and antioxidant activities of extracts from young leaves of Camellia japonica L. in Jeju (제주 동백나무 어린 잎의 페놀류 함량과 항산화 효능)

  • Jeong, Dong Won;Kim, Min Young
    • Journal of Applied Biological Chemistry
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    • v.60 no.4
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    • pp.307-311
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    • 2017
  • This study has been performed to identify the phenolic contents and antioxidant properties of methanol and ethanol extracts of young Camellia leaves in Jeju. Total polyphenol and flavonoid contents were 74.9 and 46.1 mg gallic acid equivalent/100 g, and 30.6 and 21.4 mg RE/100 g in the methanol and ethanol extracts, respectively. Based on $IC_{50}$ values, both extracts were also found to possess potent scavenging capacities of 1,1-diphenyl-2-picrylhydrazyl, nitric oxide and superoxide anion radicals, and ferrous ion chelating and reducing power activities. These results showed that young Camellia leaves is a good antioxidant source and holds promise as a natural ingredient in functional food and pharmaceutical supplement.

Antioxidative Activities of Soymilk Fermented with Bacillus subtilis

  • Seo, Kyoung-Chun;Kim, Mi-Jin;Kwon, Myung-Ja;Kim, Hyun-Ju;Noh, Jeong-Sook;Song, Yeong-Ok
    • Food Science and Biotechnology
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    • v.18 no.5
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    • pp.1298-1300
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    • 2009
  • To develop a dietary beverage with functionalities of cheonggukjang, soymilk was fermented with Bacillus subtilis and its antioxidant activities were examined. Antioxidative capacities of fermented soymilk (FS) with 5 different B. subtilis were significantly different. Among these, FS with 2829PNU015 revealed the greatest antioxidant activities. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, total antioxidant activity, and low density lipoprotein (LDL) oxidation inhibition of FS with 2829PNU015 were increased by 150, 140, and 240%, respectively, compared with those of unfermented soymilk (p<0.05). Further study for the improvement of sensory properties of FS with B. subtilis is required for a commercial production.

Drying Condition Affects Total Phenol Contents and Antioxidant Activities of Hizikia fusiformis

  • Lee, Jin-Man;Kang, Bok-Hee;Kim, Yoo-Kyeong
    • Preventive Nutrition and Food Science
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    • v.15 no.3
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    • pp.244-247
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    • 2010
  • This study was performed to evaluate the effect of drying methods on the polyphenol contents and antioxidant activities of Hizikia fusiformis. The seaweed was dried using four different conditions: vacuum drying (VD) at $20^{\circ}C$ and $30^{\circ}C$ and hot-air drying (HD) at $40^{\circ}C$ and $60^{\circ}C$. After drying, the total phenol content, DPPH and nitrite scavenging activities of the water extracts were determined. The total phenol contents were significantly (p<0.05) higher in the vacuum dried samples than the hot-air dried samples. DPPH radical was scavenged 82.67% and 83.45% by VD-20 and VD-30, and 70.44% and 71.23% by HD-40 and HD-60, respectively. At pH 1.2, Hizikia fusiformis scavenged 87.43%, 88.45%, 72.14% and 73.45% of nitrite by the treatment VD-20, VD-30, HD-40 and HD-60, respectively. Interestingly, the nitrite scavenging activity was much more reduced at pH 3.0 than at pH 1.2. Through these experiments, we were able to show that the drying conditions affect the total phenol contents and antioxidant capacities of Hizikia fusiformis.

Anti-osteoporotic and Antioxidant Activities by Rhizomes of Kaempferia parviflora Wall. ex Baker

  • Nguyen, Phuong Thao;Bui, Thi Thuy Luyen;Lee, Sang Hyun;Jang, Hae Dong;Kim, Young Ho
    • Natural Product Sciences
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    • v.22 no.1
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    • pp.13-19
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    • 2016
  • In this report, we investigated the antioxidant (peroxyl radical-scavenging and reducing capacities) and anti-osteoporotic activities of extracts and isolated constituents (1 - 16) from the rhizomes of Kaempferia parviflora Wall. ex Baker on pre-osteoclastic RAW 264.7 cells. Compound 5 exhibited significant peroxyl radical-scavenging capacity, with TE value of $8.47{\pm}0.52{\mu}M$, while compound 13 showed significant reducing capacity, with CUPRAC value of $5.66{\pm}0.26{\mu}M$, at $10.0{\mu}M$. In addition, flavonoid compounds 2, 4, 6, 8, 10, 12, and terpene compound 15 showed significant inhibition of tartrate-resistant acid phosphatase (TRAP) in NF-${\kappa}B$ ligand-induced osteoclastic RAW 264.7 cells, with values ranging from $16.97{\pm}1.02$ to $64.67{\pm}2.76%$. These results indicated that K. parviflora could be excellent sources for the antioxidant and anti-osteoporotic traditional medicinal plants.

Comparison on Physicochemical Properties and Antioxidant Activities of Commonly Consumed Coffees at Coffee Shops in Seoul Downtown (서울 시내 커피전문점에서 판매되는 커피의 이화학적 특성 및 항산화성 비교)

  • 서한석;김수희;황인경
    • Korean journal of food and cookery science
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    • v.19 no.5
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    • pp.624-630
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    • 2003
  • The physicochemical properties and antioxidant activities of commonly consumed espresso and regular coffees, at coffee shops in downtown Seoul, were investigated. Moreover, the characteristics between chain type coffee shops and owner shops were compared using t-tests. The serving temperature and volume of espresso were lower than those for regular coffees. Whereas, the pH and total acidity of espresso were higher than those for regular coffees. Coffees from chain shops, in particular, had a significantly higher pH and lower total acidity than those from owner shops. The L, a and b values of the regular coffees were higher than those of the espresso coffees. With the exception of the a value of regular coffees, all the color values of the coffees from owner shops were higher than those from the chain shops. The intensity of the brownness, soluble solid contents and total phenolic contents of the espresso coffees were about 7∼8, 8∼9 and 4 times higher, respectively, than those of regular coffees. In addition, the free radical scavenging capacities and antioxidant activities of the espresso coffees, using a chemiluminescence assay, were higher than those of regular coffees. Espresso coffees from chain shops, especially, had higher activities of free radical scavenging than those from owner shops. In conclusion, the characteristics of coffees from chain shops were significantly different from those from owner coffee shops.

Effect of genotypes on macronutrients and antioxidant capacity of chicken breast meat

  • Lengkidworraphiphat, Phatthawin;Wongpoomchai, Rawiwan;Taya, Sirinya;Jaturasitha, Sanchai
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.11
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    • pp.1817-1823
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    • 2020
  • Objective: The increasing consumer awareness of food, which can provide health benefits and potentially aid disease prevention, has become the driving force of the functional food market. Accordingly, the aim of this study was to investigate the effects of chicken genotype on the macronutrient content, bioactive peptide content, and antioxidant capacity within different breast meat. Methods: In this experiment, three genotypes of chicken, Thai indigenous, black-boned, and broiler (control), were reared with commercial feed under the same conditions. Thirty chickens were slaughtered at typical market age and the breasts were separated from the carcass to determine macronutrient content using the AOAC method. The antioxidant capacities of the chicken breasts were evaluated by in vitro antioxidant assays and the protein pattern was investigated using gel electrophoresis. Carnosine and anserine, which have antioxidant properties in animal tissue, were determined using high performance liquid chromatography. Results: The results showed that breast meat from Thai indigenous chickens had a greater macronutrient content and higher antioxidant capacity compared with the other genotypes (p<0.05). The protein pattern was similar between genotypes, however Thai indigenous chickens had the greatest myosin and actin content (p<0.05). In addition, carnosine and anserine values were greatest in the black-boned and Thai indigenous chickens compared with the broiler genotype (p<0.05). Conclusion: Thai indigenous chicken breast meat may be classified as a functional food as it has good nutritional value and is rich in antioxidant peptides.

Antioxidant Activities of Extracts from Various Kimchi with Heat and Non-heat Treatment of Salt-Fermented Anchovies (가열 및 비가열 처리 액젓으로 제조한 김치 추출물의 항산화 활성)

  • Kang, Hyun Woo;Cho, Young-Jae
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.2
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    • pp.257-263
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    • 2013
  • The free radical scavenging activities of extracts from kimchi, as well as various antioxidant activities, such as 2,2-azinobis-( 3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing antioxidant power (FRAP) were examined. Specifically, the ABTS radical scavenging and FRAP activities of ethanolic extracts from kimchi (kimchi with non-treated salt fermented anchovy, treated non-heat salt fermented anchovy, treated heat salt fermented anchovy and commercial salt-fermented anchovy) were $0.749{\pm}0.26$, $0.895{\pm}0.15$, $0.758{\pm}0.12$, and $0.769{\pm}0.02$ mM Trolox eq./mg extract and $0.727{\pm}0.11$, $0.901{\pm}0.03$, $0.811{\pm}0.17$, and $0.843{\pm}0.16$ mM $FeSO_4$ eq./mg extract, respectively. In addition, the antioxidant activity of extract from kimchi was estimated in vivo. Mice were randomly divided into six groups (n=6), and treated with LPS for 10 days, which resulted in lower antioxidant capacities as well as increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) values in the blood serum. Furthermore, antioxidant enzymes (catalase and superoxide dismutase) in blood serum increased in the presence of the kimchi. The present results reveal antioxidant activities from kimchi and encourage their application for the food industry and cooking.

Effects of commonly used infusion method on catechin content and antioxidant capacities of pure green tea packaged in tea bags (음용 조건으로 추출한 티백 형태 순수 녹차의 카테킨 함량 및 산화방지능)

  • Kim, Mi-Seon;Rha, Chan-Su;Kim, Dae-Ok
    • Korean Journal of Food Science and Technology
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    • v.51 no.4
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    • pp.356-360
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    • 2019
  • To evaluate antioxidant capacities and total phenolic, flavonoid, and catechin contents of pure green tea packaged in tea bags, green tea bags of 10 commercial brands were purchased and analyzed. Considering commonly used infusion methods, green tea bags were extracted using 150 mL of spring water at $70^{\circ}C$ for 2 min. Ten brands of green tea showed a wide range of total phenolic content (46.3-93.9 mg gallic acid equivalents/g), total flavonoid content (10.3-21.7 mg catechin equivalents/g), and antioxidant capacities (88.8-175.6 mg and 87.9-183.5 mg vitamin C equivalents/g for DPPH and ABTS assays, respectively). Total catechin content of green tea ranged from 33.8-74.7 mg/g, which corresponded to 43.0-108.3 mg/150 mL of cup. Collectively, these results suggest that daily consumption of 3.8-12.5 cups of green tea packaged in tea bags meets the recommended daily intake of catechins in green tea extract as a health functional foods in Korea.