• 한국식품위생안전성학회지
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• 제29권1호
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• pp.47-52
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• 2014

This study was performed to evaluate antimicrobial resistance of food-borne pathogens isolated from retail meat in Korea. A total of 157 samples of beef, pork, and chicken were collected and analyzed for E. coli, Salmonella, Campylobacter. Resistances to tetracycline were declined in accord with reduced usage of tetracycline in live stock production. E. coli stains from chicken meat had higher multi-drug resistance ratio than strains from other meat. One extended spectrum beta lactamase (ESBL) producing E. coli and two ESBL producing Salmonella were identified in this study. ESBL producing Salmonella strains were confirmed to carry CTX-M-1 type genes.

• 한국동물위생학회지
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• 제36권2호
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• pp.111-119
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• 2013

The aim of this study was to investigate the isolation frequency and antimicrobial resistance of Escherichia coli (E. coli) and Enterococcus spp. from domestic beef and pork on sale in Seoul, Korea. A total of 106 (10.4%) E. coli and 114 (11.2%) Enterococcus spp. from 635 domestic beef and 381 pork samples were isolated and examined for susceptibility to 15 and 11 antimicrobial agents, respectively. The most frequent antimicrobial resistance observed in E. coli isolates was to ampicillin (38.6%), followed by streptocmycin (34.9%) and tetracycline (32.0%). The most frequent antimicrobial resistance in E. faecium isolates was to erythromycin (53.8%) and rifampin (46.1%), and in E. faecalis isolates was to tetracycline (55.7%) and rifampin (55.0%). Among the isolates, multi-drug resistant (MDR) E. coli and Enterococcus spp. strains showing resistances to more than two antimicrobial agents tested were 10.4% and 11.2%, respectively. As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in future.

• International journal of advanced smart convergence
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• 제11권4호
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• pp.227-239
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• 2022

Purpose: In our study, in order to find lactic acid bacteria (LAB) with multi-drug resistance to antibiotics, we isolated 140 strains from 15 types of kimchi commercially available in Korea and 20 types of Kimchi made at home from January to December in 2016, and investigated their H₂O₂ generating ability and multi-drug resistance to antibiotics. Methods: In order to observe the H₂O₂ generation ability of LAB, we performed the experiment with methods such as Rabe, Hillier, and Kang. To test the antibacterial susceptibility of LAB, we used the disc agar diffusion method using MRS agar (Difco, USA) according to the CLSI and WHO test methods. There are 18 types of antibiotic discs used. Results: Out of the total numbers of 140 strains, 6 strains of Ent. Faecium, 25 strains of L. plantarum, 1 strain of L. rhamnosus, 3 strains of L. sakei, 1 strain of L. acidophilus, 1 strains St. thermophilus, and 7 of unidentified strains generated H₂O₂. The antibiotic susceptibility of Ent. Faecium indicated SXT, OX, NA, and E; and the antibiotic susceptibility of L. plantarum indicated NA; and the antibiotic susceptibility of St. thermophilus indicated NA, CC, RA, CTT, CM, and P ; and the antibiotic susceptibility of L. rhamnosus indicated SXT, VA, NA and CTT; and the antibiotic susceptibility of 6 strains of L. sakei indicated SXT, OX, NOR, NA, CTT and CIP, all indicating antibiotic resistance. In the case of multi-drug resistance to antibiotics for 53 strains of L. antarum, 8-drug resistance was the most common with 25 strains, followed by 7-drug-resistant strains with 18 strains, 9-drug-resistant strains with 4 strains, 6-drug-resistant strains with 3 strains, 5-drug-resistant strains with 2 strains, and 17-drug-resistant strains with 1 strain. In the case of multi-drug resistance to antibiotics for Ent. Faecium 27 strains, 9-drug resistance was most commonly identified as 9 strains, 8-drug resistance was identified as 6 strains, 7- and 11 drug resistances were identified as 4 strains each, and 4- and 6-drug resistances were identified as 1 strain each. Conclusion: Ent. Faecium, L. plantarum, L. rhamnosus, L. sakei, and St. thermophilus, shown to have anantibacterial activity in previous studies on LAB and shown to have and H₂O₂ generating ability, antibiotic resistance and multi-drug resistance in this study, are expected to be able to play an excellent role for long-term inpatients to use as an alternative to antibiotics and to cope with emerging antibiotic resistance.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.517-525
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• 2023

다제내성 P. aeruginosa (MRPA)의 출현과 확산은 전 세계적으로 심각한 문제가 되었다. 특히 metallo-β-lactamases (MBLs)의 carbapenem 고도내성 관여 정도는 심각한 수준이며, 특히 P. aeruginosa는 장내세균 속 균종과는 달리 새로운 클론들이 지속적으로 나타나고 기존에 확산되었던 우세 클론을 대체하는 과정이 매우 빠르게 진행되고 있다. 이에 본 연구에서는 2017년 9월부터 2019년 9월까지 부산의 한 종합병원에서 다양한 의료용 시료로부터 분리된 18균주의 P. aeruginosa 균주에 대한 항균제 내성 유전자 분석과 DNA 염기서열 분석을 통한 Integron의 유전자 카세트 분석 및 접합에 의한 Plasmid 전달 분석을 수행하며 이에 대한 역학관계를 조사하고자 하였다. 18균주 모두 XDR 표현형을 보이는 균주였으며, Colistin(100%)을 제외한 대부분의 항생제에 내성을 나타냈으나, aztreonam(22.2%), ceftazidime(16.6%)에 일부 감수성을 보였다. 균주의 66.7%는 다양한 항균제 내성을 나타내는 Class1 integron을 가지고 있었으며, 접합에 의한 Plasmid전달도 성공적으로 이루어졌다(83.3%). 이는 이전의 장내세균에 대한 연구결과보다 25.8% 상향한 결과를 보여 공중보건에 대한 심각한 역학관점의 고찰을 필요로 한다. 특히, IMP-6 ST235 (66.7%)가 주를 이루며, VIM-2 ST357 (16.7), IMP-1 ST446 (16.7)이 확인되었다. 흥미롭게도, 국내에서는 아직까지 보고된 바가 없는 IMP-1 생성 ST446의 확인은 또 다른 MRPA 고위험 클론의 생성과 유행이라는 관점에서 주목할 만하다.

• Journal of Korean Biological Nursing Science
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• 제20권1호
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• pp.38-46
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• 2018

Purpose: The aim of this study was to verify the effects of daily 2% chlorhexidine gluconate (CHG) bathing on the acquisition of multidrug-resistant organisms (MDRO) and healthcare-associated infection (HAI) in a medical intensive care unit (MICU). Methods: The study was a randomized controlled group posttest only design, involving 91 patients in MICU at a tertiary hospital (47 patients in the experimental group and 44 patients in the control group). The 2% CHG bathing was performed daily according to bathing protocol to the patients in the experimental group, and traditional bath was performed every three days to those in the control group. Fisher's exact test and x2 test were used to analyze the data. Results: MDRO were found in 6 patients of the experimental group and in 15 patients of the control group. The difference was statistically significant (p= .016). HAI occurred in 2 patients of the experimental group and in 7 patients of the control group. The difference was not statistically significant (p= .084). Conclusion: The results confirmed that daily bathing with CHG was effective in reducing the incidence of MDRO acquisition. Therefore, it is expected that daily bathing with CHG will be used as an effective nursing intervention to reduce the incidence of MDRO acquisition.