• Title/Summary/Keyword: Antifungal substance

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Antifumgal Activity and Identification of an Actinomycetes Strain Isolated from Mummified Peaches (복숭아 미이라과로부터 분리한 방선균의 항균 활성 및 동정)

  • 임태헌;이정목;장태현;차병진
    • Microbiology and Biotechnology Letters
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    • v.28 no.3
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    • pp.161-166
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    • 2000
  • Antifungal Activity and Identification of an Actinomycetes Strain Isolated from Mummified Peaches. Lirn, Tae Heon*, Jung Mok Lee, Tae Hyun Chang, and Byeongjin Chal. *Research Institute of Plant Nutrient, Oaeyu Co, Inc. Kyongsan 712-820, Korea, 1 Department of Agricultural Bi%g'f Chungbul< NatJ"onal Univershy, Cheongju 367-763, Korea - An actinomycetes strain which produced chitinase, urease, and antifungal substances to MoniliniaJhtcticola was isolated from peaches mununified by Moniliniafructicola. The strain TH-04 was identified as Streptomyces sp. based on cultural and lTIOIphological characteristics, cell wall diaminopimelic acid, and sugar patterns ofwhole~cell extracts. Streptomyces sp. TH~04 showed antifungal activity to several fungi including Moniliniafructicola, Colletotrichum gloeosporioides, Magnaponhe grisea, Rhizoctonia solani, Phytophthora capsici, Altemaria kikuchiana, Fusarium solani, and Fusarium O),ysporum. The optimum cultural conditions for the production of antifungal substances were $20^{\circ}C$pH 7, and 7 days.

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Antifungal Action of Ginkgo biloba Outer Seedcoat on Rice Sheath blight

  • Oh, Tae-Seok;Koo, Han-Mo;Yoon, Hei-Ryeo;Jeong, Nam-Su;Kim, Yeong-Jin;Kim, Chang-Ho
    • The Plant Pathology Journal
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    • v.31 no.1
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    • pp.61-66
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    • 2015
  • From study of antifungal actions on the rice sheath blight by using the extract of Ginkgo biloba outer seedcoats, we found that the extracts of Ginkgo biloba outer seedcoats of all treatment concentrations had inhibited the rice sheath blight. Among them, the most effective concentration was 250 mg/l at which the growth of microbe was 26 mm and even at the packaging test, when sprayed the G. biloba outer seedcoats at the level of 250 mg/l, the damage rate of the rice sheath blight was identified as 13%. As a result investigating the antifungal activity by separating polysaccharides from G. biloba outer seedcoats, it showed that the clear zone of 14 mm or more was formed at the concentration of 250 mg/l or higher. Based on these results, we concluded that the G. biloba outer seedcoat is a natural substance with the antifungal activity on the rice sheath blight.

In Vitro Antifungal Activity of HTI Isolated from Oriental Medicine, Hyungbangjihwang-tang (형방지황탕으로부터 분리된 HTI의 항진균활성에 대한 연구)

  • Sung, Woo-Sang;Seu, Young-Bae;Lee, Dong-Gun
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.273-279
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    • 2009
  • Hyungbangjihwang-Tang (HT), an Oriental herbal formula, has been known to play a role which helps to recover vigor of human in the Orient. In this study, antifungal substance (HTI) was purified from the ethyl-acetate extracts of HT by using $SiO_2$ column chromatography and HPLC, and the antifungal effects of HTI and its mode of action were investigated. By using a broth micro-dilution assay, the activity of HTI was evaluated against fungi. HTI showed antifungal activities without hemolytic effect against human erythrocytes. To confirm antifungal activity of HTI, we examined the accumulation of intracellular trehalose as stress response on toxic agents and effect on dimorphic transition in Candida albicans. The results demonstrated that HTI induced the accumulation of intracellular trehalose and exerted its antifungal effect by disrupting the mycelial forms. To understand its antifungal mode of action, cell cycle analysis was performed with C. albicans, and the results showed HTI arrested the cell cycle at the S phase in yeast. The present study indicates that HTI has considerable antifungal activity, deserving further investigation for clinical applications.

Antifungal Performance of Hwangtoh Mortars with Natural Antifungal Substances (천연 항균물질을 이용한 황토모르타르의 항곰팡이 성능 평가)

  • So, Hyoung-Seok;Kim, Jung-Woo;Lee, Bo-Ram;So, Seung-Young
    • Journal of the Korea Concrete Institute
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    • v.27 no.1
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    • pp.75-83
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    • 2015
  • This study discusses the long-term antifungal effect and antifungal performance of Hwangtoh mortars with various natural antifungal substances on five types of mold: Chaetomium globosum, Aspergillus niger, Aureobasidium pullulans, Gliocladium virens, and Penicillium pinophilum, which can be easily detected in the indoors and outdoors of buildings in damp environments. The antifungal performances of various natural antifungal substances extracted from Marjoram, Phytoncide, Thyme, Ginkgo leaves, and Chitosan (oligosaccharide) were investigated on the five types of mold, as a basic experiment. Using the natural antifungal substances selected for the basic experiment, antifungal mortars were made, and their antifungal performance and long-term antifungal effects were also investigated. The results clearly showed that the marjoram extract and their associated mortars had excellent antifungal performance. Also, their long-term antifungal effects were outstanding and at an equivalent level to those of the mortars with organic chemical antifungal agents. The optimum addition rate of the marjoram extract in the mortar was 5% by mass of binder. It was also shown that the phytoncide extract in the mortar needed an addition rate of more than 10% according to the mass of binder, considering the long-term antifungal effect and the antifungal performance.

Separation and Activity Test of Antifungal Substance from C. japonica Extract (황련 추출물로부터 항균활성물질의 분리 및 활성 검정)

  • Chung, Ill-Min;Paik, Su-Bong
    • Analytical Science and Technology
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    • v.10 no.2
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    • pp.153-159
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    • 1997
  • This study was conducted to test antifungal activity and to separate bioactive compound using C. japonica extract which was shown the most effective control on the B. berengeriana, G. cingulata and P. expansum. $EC_{50}$ on the test pathogene of C. japonica extract was $400{\sim}500{\mu}g/mL$. Bioactive compound analyzed by TLC and HPLC method was identified as a kind of alkaloids, berberine, and the content was 81.14%.

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Concanamycin B, Active substance Against Phytophthora capsici Produced by Streptomyces neyagawaensis 38D10 Strain (Streptomyces neyagawaensis 38D10 균주가 생산하는 concanamycin B의 항고추역병 활성)

  • Kim, Chang-Jin;Lee, In-Kyoung;Yun, Bong-Sik;Yoo, Ick-Dong
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.322-328
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    • 1993
  • During the screening of antifungal compounds from microbial secondary metabolites to control phytophthora blight of red pepper caused by Phytophthora capsici, a soil isolate, strain 38D10 was selected. Based on taxonomic studies, this strain was identified as Streptomyces neyagawaensis. The antifungal compound was purified from culture broth by HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, HPLC and identified as concanamycin B by UV. $^1H$-NMR, $^{13}C$-NMR, SIMS analysis. Concanamycin B has strong antifungal activity against some phytopathogenic fungi but not antivacterial activity and preventive value were 50% and 100% at 125ppm and 250ppm in pot assay.

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In Vivo Disease Control Efficacy of Isoquinoline Alkaloids Isolated from Corydalis ternata against Wheat Leaf Rust and Pepper Anthracnose

  • Han, Jae Woo;Shim, Sang Hee;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Hun;Choi, Gyung Ja
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.262-266
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    • 2018
  • Phytochemicals have been considered as alternatives for synthetic fungicides because of their biodegradability and low toxicity. In this study, we found that the methanolic extract of Corydalis ternata suppressed the development of plant diseases caused by Puccinia triticina and Colletotrichum coccodes. As the antifungal substance, three isoquinoline alkaloids (dehydrocorydaline, stylopine, and corydaline) were isolated from C. ternata. These active compounds also exhibited in vivo antifungal activity against P. triticina and C. coccodes. Taken together, our results suggest that C. ternata and its active compounds can be used to control plant diseases.

Allelopathic Effects on Seed Germination and Fungus Growth from the Secreting Substances of Some Plants (수종식물의 분비물질이 종자 발아와 균류 생장에 미치는 알레로파시 효과)

  • 이호준;김용옥;장남기
    • The Korean Journal of Ecology
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    • v.20 no.3
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    • pp.181-189
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    • 1997
  • Phenolic compounds from 7 species of naturalized invader species and Korean wild plants were analyzed by high performance liquid chromatography. Eleven phenolic compounds including benzoic acid were identified. The extract of naturalized plants was significantly more inhibitory to seed germination and seedling growth of the both naturalized and Korean wild plants. The content of total phenolic compounds in each extract were 43.5 mg/l in Ailanthus altissima as the maximum amount and 25.5 mg/l in Phytolacca americana as the minimum. Phytotoxic substances of ethanol extracts was investigated for antifungal activity against 23 selected fungus species. The antifungal activity of Phytolacca americana showed the greatest clear zone of 23 mm in Aspergillus awamori and its activity had an effect against 6 fungus species. Ailanthus altissima formed the greatest clear zone of 26 mm in Erwinia carotovora sub. sp. carotovora and had an effect agsinst 2 fungus species.

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Antifungal Activity of Valinomycin, a Peptide Antibiotic Produced by Streptomyces sp. Strain M10 Antagonistic to Botrytis cinerea

  • Park, Cheol-Nam;Lee, Jung-Min;Lee, Dong-Ho;Kim, Beom-Seok
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.880-884
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    • 2008
  • A strain of Streptomyces sp. (M10) antagonistic to Botrytis cinerea was isolated from orchard soil obtained from Jeju Island, Korea. An antifungal substance (CN1) was purified from the culture extracts of the strain, and then identified as valinomycin through extensive spectroscopic analyses. Valinomycin showed potent in vitro antifungal activity against Botrytis cinerea and also in vivo control efficacy against Botrytis blight development in cucumber plants. Overall, the disease control efficacy of valinomycin was similar to that of vinclozolin, a commercial fungicide. This study provides the first report on the disease control efficacy of valinomycin against Botrytis blight.

Antifungal Activity or Coptis japonica Root-stem extract and Identification of Antifungal Substances (황련추출액의 항균활성과 항균성물질의 동정)

    • Korean Journal of Plant Resources
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    • v.12 no.4
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    • pp.260-268
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    • 1999
  • Crude extract of Coptis japonica root-stem was evaluated for antifungal activity against Phytophthora capsici, Fusarium oxysporum, Colletotrichum dematium, Colletotrichum truncatum, Botrytis cinerea, Botryosphaeria dothidea and Alternaria porri, and antifungal active compound from the extract was identified. In addition, the usefulness of the extract for some plants disease control was investigated. Crude extract of C. japonica root-stem exhibited antifungal activity against P. capsici, F. oxysporum, C. dematium, B. cinerea, B. dothidea and A. porri. Antifungal activity of the substance isolated from C. japonica root-stem was similar to a standard chemical berberine-Cl. Red-pepper fruit rot, sesame stem rot and welsh-onion alternaria leaf spot were effectively controlled by the crude extract of C. japonica root-stem. Phytotoxicity was not observed in the red-pepper and welsh-onion leaves and red-pepper and strawberry fruits with exogenous foliage application of the crude extract. Seeds germination and radicle growth of red-pepper and sesame were inhibited by the crude extract of C. japonica root-stem. 4.24g of yellowish compound per 100g of C. japonica root-stem was obtained. The compound was identified as berberine-Cl by HPLC.

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