• 한국작물학회:학술대회논문집
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• 한국작물학회 2000년도 춘계 학술대회지
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• pp.132-133
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• 2000

• Journal of Plant Biotechnology
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• 제47권3호
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• pp.203-208
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• 2020

The involvement of antifreeze proteins (AFPs; type I and III) in the germination of low temperature-treated petunia seeds (cv. 'Mirage Rose') was investigated. The addition of AFPs (300 or 500 ㎍/l) in low-temperature treatment significantly promoted the germination of seeds compared with that in which AFPs were not added. Among all treatments, treatment with AFP I added at 300 ㎍/l showed the highest germination percentage and improved plant growth. The expression levels of antioxidant-related genes such as superoxide dismutase, peroxidase, and proline synthesis were associated with the germination of low temperature-treated seeds. Overall, this study demonstrated that AFP I may potentially function as a cold-protective agent for the germination of low temperature-treated seeds.

• 한국수의병리학회지
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• 제2권2호
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• pp.147-151
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• 1998

A five-month old female Great Dane with a history of depression and convulsion was submitted to the Pathology and Diagnosis Reference Division of National Veterinary Research and Quarantine service. The dog had ingested concentrated antifreeze and died 12 days after the sudden onset of clinical signs. Main gross lesion was limited to swollen kidneys with diffusely fine granularity on the cut surface. Principle microscopic lesions were focused on the kidneys including dilated Bowman's space, thickened glomerular basement membrane, many light yellowish crystals, and proteinaceous casts in some tubules. Some neutrophils and lymphocytes were focally infiltrated in interstitium of the cortex and medulla. Crystals were angular or quite irregular in shape and arranged in sheave or rosette form. Under polarized light, the crystals were birefringent, which is typical of calcium oxalate crystals. Crystals were more concentrated in the cortex than in the medulla. A few crystals were also observed in the small blood vessels of the brain. Based upon the history of ingestion of antifreeze and the identification of calcium oxalate crystals in the tissue sections of the kidney and brain, this case was diagnosed as ethylene glycol toxicosis in a dog.

• Plant Resources
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• 제7권2호
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• pp.147-154
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• 2004

Antifreeze proteins (AFPs) accumulate in the leaves of barley during cold acclimation, where they may inhibit ice recrystallization and produce freezing resistance of the plant. Four parental diallel crosses of the barley varieties were used to determine the heritability of AFPs and the relationship between the accumulation level of AFPs and freezing resistance. The concentration of apoplastic proteins in the cold-acclimated leaves was increased in the mean by four-fold over as compared with that of nonacclimated. The diallel cross analyses revealed that the gene of Sacheon 6 was dominant and those of Reno and Dongbori 1 were recessive. The AFPs had high narrow-sense heritabilities. The general combining ability effects of Reno and Dongbori 1 were much higher than the other parents. The bands of 32-kD for GLP, 35-& 28-kD for CLP and 25-, 22- & 16-kD for TLP were observed in the apoplastic extracts from cold-acclimated plants, but there were no clear differences between the parents and Fl hybrids. The concentrations of AFPs were significantly correlated with the degree of freezing resistance, indicating that the concentration of AFPs in the plant is the very important factor for freezing resistance.

• Journal of Advanced Marine Engineering and Technology
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• 제38권9호
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• pp.1027-1031
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• 2014

외기냉수냉방 시스템은 공조나 산업공정에 사용되는 냉수를 저온의 외기를 이용하여 냉각하는 시스템으로 에너지를 절감하기위해 사용되며, 냉각시스템과 외기와 냉수가 열교환을 하는 드라이쿨러로 구성되어있다. 외기냉수냉방 시스템은 지속적인 냉방이 필요한 곳에 에너지절감효과가 뛰어나나 국내의 경우, 외기냉수냉방 시스템에 설계 기준과 자료가 미비하다. 따라서 본 연구에서는 상용프로그램 HYSYS를 이용하여, 드라이쿨러를 이용한 외기냉수냉방 시스템의 요소변화에 따른 성능을 비교 분석했으며 그 결과는 다음과 같다. 드라이쿨러의 냉각능력은 외기온도, 요구냉수온도, 열교환기의 LMTD (Logarithmic Mean Temperature Difference, 대수평균온도차)에 따라 변한다. 드라이쿨러는 외기온도가 낮을 수록 그 냉각능력이 증가하며, LMTD가 낮은 드라이쿨러가 LMTD가 높은 것에 비하여 고온의 외기온도에서 사용이 가능하였다. 외기온도가 극히 낮은 지역에서는 글리콜이 함유된 부동액을 사용해야하며 국내의 외기온도를 고려하였을 때, 글리콜농도 34%의 부동액이 적절한 것으로 판단된다. 냉동기에 적용될 작동유체의 경우, R22, R134a, R407C의 압축일을 비교했으며, R134a가 환경관련 규제와 에너지 사용측면에서 우수한 냉매로 사료된다.

• Journal of Plant Biotechnology
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• 제44권4호
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• pp.388-393
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• 2017

We report an Agrobacterium-mediated transformation procedure optimized for 'Kyoho' that is a major table grapevine cultivar in Korea, and its transgenic plants with antifreeze protein gene of carrot (DcAFP). The full length of DcAFP coding region in accordance with the previous report was isolated from young leaves of carrot and recombined into a plant transformation vector. Ethylene inhibitors such as silver nitrate and aminoethoxyvinylglycin (AVG) supplemented in a co-cultivation medium distinctly increased frequency of shoot regeneration when explants were sub-cultured in a selection medium: particularly ten-fold higher in treatment with 0.1 mg/L AVG than one without ethylene inhibitor. Among various antibiotics and their concentrations, the combination of 150 mg/L cefotaxime plus 150 mg/L $Clavamox^{TM}$ was selected for elimination of Agrobacterium cells in addition to minimization of adverse effect on shoot regeneration, while 50 mg/L kanamycin monosulfate effectively suppressed regeneration of non-transgenic shoots. Applying the elucidated culture condition, we finally obtained a total of 5 transgenic 'Kyoho' plantlets with DcAFP, of which integration with the grapevine genome and transcription was confirmed by nucleic acid analyses.

• 한국산학기술학회논문지
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• 제16권3호
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• pp.1594-1599
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• 2015

세계 자동차 산업은 지난 100여년간의 내연기관의 시대에서 환경, 에너지 그리고 IT기술들이 접목된 친환경, 첨단자동차의 시대로 변모해가고 있다. 지금까지 자동차의 연구는 새로운 기술에 대한 연구 및 개발에 주력해 왔다. 자동차의 새로운 기술개발도 중요한 사항이지만, 최첨단 기술이 적용된 자동차 이전에 이미 사용되어지고 있는 자동차의 성능 및 환경개선 문제도 중요한 시점이 되었다. 새로운 기술개발 뿐 만아니라 기존의 기술에 대한 효과적인 적용 또한 중요한 사항이다. 부동액 기술 또한 자동차 기술의 중요한 부분이다. 본 연구는 부동액의 효과적인 적용을 위하여 자동차 엔진 냉각수에 함유된 부동액의 함유량과 냉각수의 종류(수돗물, 증류수, 지하수)에 따라 자동차 엔진 온도와 성능에 미치는 영향을 파악하고 원인을 규명하는 실험적 연구이다. 냉각수의 어는점 -10, -20, -30, -40, $-50^{\circ}C$에서 다이나모 성능시험과 수치해석을 실시하였다. 냉각수(증류수) 어는점 $-10^{\circ}C$에서 측정 기준점에서 최고 성능 71.112, 99.622hp를 나타냈다.

• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1777-1784
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• 2020

To increase the availability of microalgae as producers of valuable compounds, it is necessary to develop novel systems for gene expression regulation. Among the diverse expression systems available in microalgae, none are designed to induce expression by low temperature. In this study, we explored a cold-inducible system using the antifreeze protein (AFP) promoter from a polar diatom, Chaetoceros neogracile. A vector containing the CnAFP promoter (pCnAFP) was generated to regulate nuclear gene expression, and reporter genes (Gaussia luciferase (GLuc) and mVenus fluorescent protein (mVenus)) were successfully expressed in the model microalga, Chlamydomonas reinhardtii. In particular, under the control of pCnAFP, the expression of these genes was increased at low temperature, unlike pAR1, a promoter that is widely used for gene expression in C. reinhardtii. Promoter truncation assays showed that cold inducibility was still present even when pCnAFP was shortened to 600 bp, indicating the presence of a low-temperature response element between -600 and -477 bp. Our results show the availability of new heterologous gene expression systems with cold-inducible promoters and the possibility to find novel low-temperature response factors in microalgae. Through further improvement, this cold-inducible promoter could be used to develop more efficient expression tools.

• 한국태양에너지학회 논문집
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• 제36권2호
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• pp.73-78
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• 2016

This study investigates the main factors of fire prevention, such as spontaneous ignition and flash point, while using Diehylene Glycol (DEG) as the antifreeze for automobile and industrial machines. Our results show the flash point of $142^{\circ}C$ and the minimum ignition temperature of $388^{\circ}C$ in the range of $130{\mu}{\ell}{\sim}150{\mu}{\ell}$. By increasing temperature to ignite in 1 second, an instantaneous ignition temperature of the sample is $569^{\circ}C$ in the sample amount of $140{\mu}{\ell}$.

• Journal of Advanced Marine Engineering and Technology
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• 제26권2호
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• pp.240-247
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• 2002

Recently, a thermal energy storage system has been developed actively fur the purpose of saving energy and reducing the peak electrical demand. Especially, ice slurry is a promising working fluid for low temperature energy storage systems. A flow of ice crystals has a large cooling capacity as a result of the involvement of latent heat. However, there are still problems related to the recrystallization of ice crystals for realizing long term storage and long distance transportation. To find improvements fur this, a method for the creation of ice crystals resistant to recrystallization has been proposed and researched by the use of an antifreeze protein (AFP) solution etc. In the present study, it has been investigated the growth of ice crystal in several kinds of water solution added non-ionic surfactant. The results shows that size of ice crystal was smaller with increasing in added surfactant. And ice crystal was not increased with added surfactant.