• Archives of Pharmacal Research
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• 제23권3호
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• pp.240-242
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• 2000

The immunomodulating activity of a polysaccharide isolated from Morus alba (PMA) root bark was examined in murine splenic lymphocytes. PMA enhanced proliferation of splenic lymphocytes in a synergistic manner in the presence of mitogens. However, PMA suppressed pri-may IgM antibody production from B cells, which was activated with lipopolysaccharide, a polyclonal activator, or immunized with a T-cell dependent antigen sheep red blood cells. Our observations showed that the immunomodulating activity of PMA increased lymphocyte proliferation and that PMA decreased antibody production from B cells, which was distinct from those of other plant-originated polysaccharides.

• Journal of Ginseng Research
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• 제12권1호
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• pp.63-67
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• 1988

인삼사포닌 분획물들(total saponin, diol saponin and triol saponin)이 생쥐의 항체생성에 미치는 영향과 면역억제의 회복에 미치는 영향 그리고 혈청총단백질의 생성에 미치는 영향에 대하여 알아본 결과 아래와 같은 결론을 얻었다. 1. Total saponin은 10mg/kg/day, diol saponin은 10mg/kg/day, triol saponin은 5mg/kg/day로 생쥐에 투여하였을 때 순환성 항체가 가장 많이 생성되는 것으로 나타났다. 2. CY로 면역을 억제시킨 상태에서는 인삼사포닌을 투여하여도 순환성 항체의 생성이 대조군과 차이가 없는 것으로 나타났다. 3. 인삼사포닌은 항체생성과 혈청총단백질의 증가에 서로 다른 농도로 작용을 나타낸다.

• 한국미생물·생명공학회지
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• 제34권1호
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• pp.47-51
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• 2006

Sodium butyrate (NaBu) is used as an enhancer for the production of recombinant proteins in Chinese hamster ovary (CHO) cells. However, NaBu is well-known for its cytotoxic effect, thereby inducing apoptosis. CHO cells which had been engineered to express a humanised anti-HBV antibody were cultured using serum-free medium, Ex-cell 301. From a seeding density of $2{\times}10^5$ cells/ml, CHO cells grown with serum-free medium reached a maximum cell density of $1.3{\times}10^6$ cells/ml after 9 days in culture and produced a maximal antibody concentration of 130 mg/l after 13 days in culture. In the perfusion culture system, CHO cells producing anti-HBV antibody grown in an 7.5 1 bioreactor seeded with $2{\times}10^5$ cells/ml reached a maximal antibody concentration of 85 mg/1 after 720 h in culture. The addition of 0.3 mM NaBu and lowering culture temperature to $33^{\circ}C$ elongated the culture period to 60 days and increased the production yield by 2-fold, compared to control culture.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1993년도 학술대회지
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• pp.84-93
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• 1993

It has been reported that ginseng is effective in the central nervous system, immune system, and the strong inflammatory responses. However, there has been no research report yet about the effect of ginseng on allergic hypersensitivity reactivity. To confirm the ginseng effects on the release of mediators(histamine. leukotrienes etc.) which cause the hypersensitivity reactivity and inflammatory response, we used actively sensitized guinea pig airway tissues by utilizing the superfusion technique. In this procedure. the contractile response and mediators released after antigen stimulation of sensitized tissues, and IgG and IgE antibody products were measured in sera of immunized animals. Then the results of the controll group were compared to those of ginseng pretreatment groups. In the total saponin(TS) and panaxatriol(PT) pretreatment, histamine release decreased by $20\%$ in the tracheal tissues after active sensitization by ovalbumin(OVA, 10mg/kg), but in the lung parenchyma, histamine release decreased by $40\%.$ Panaxadiol(PD) significantly decreased histamine release by $40\%$ in the both tissues after active sensitization. TS, PT and PD of ginseng poorly blocked leukotrienes (LTs) and prostagrandin $D_2(PGD_2)$ release(less than $10\%$). Ginseng TS and PT had no effect on the serum IgG antibody production by ovalbumin, whereas PD significantly increased serum IgG antibody contents(approximately by 2 times). However, $IgG_1$ antibody products in the serum of guinea pig actively sensitized with ovalbumin after PD pretreatment were decreased, compared to that with ovalbumin alone. IgE antibody production by passive cutaneous anaphylaxis(PCA) titer in the TS pretreatment increased 3 times more than in the absence of TS(PCA titer by PT was not detected). These studies show that some ginseng saponins can in part act to inhibit mediator release in antigen - induced airway smooth muscle by inducing the IgG antibody production which has been changed in the specificity.

• 한국가축번식학회지
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• 제15권3호
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• pp.179-187
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• 1991

This experiment was carried out to develop a new technique of identifying XX of XY-bearing bisected embryos prior to implantation by immunological method. H-Y antiserum prepared in inbred Wastar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentraton or affected H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and culture rate. However, production of live young or sex rates of male and female from embryos transferred with psudopregnant. The biological test with the morula stage embryos showed that H-Y antibody was formed in all female rats immunized with spleen cell, but it was formed only in 80% female rats immunized with the antigen. When the bisected mouse embryos were cultured in vitro for 5~6 hours in morula stage, of 457 bisected embryos 81.4% of then were developed to the blastocyst stage. When the concentration rate of complement to H-Y antiserum varied from 1.0~5.0${mu}ell$, the lysis-rate of embryo was 19.5 to 67.3%. The concentration rate of complement did not influence the lysis-rate of embryos(P<0.05). The morphology embryos of bisected, zona-free and intact embryos showed the embryos lysis rate of 58.6, 42.7 and 48.5% respectively(P<0.05). Pregnancy rate were 50.0, 45.5 and 57.1% in psudopregnant recipient transferred with bisected, zona-free and intact blastocyst embryos. However, production of live youngs, sexual rate of male or female was 24(50.0:50.0), 22(45.5:55.5) and 36(58.3:41.7)mice, but affected and non affected half embryos with H-Y antiserum treatment was 23.1 and 26.7%. Also production of live youngs and sexual rate was 14(92.9:7.1) and 17(17.6:82.4)mice in affected and non affected half embryos in H-Y antiserum treatment(P<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.366-370
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• 2004

One hundred and sixty day-old Hainan chicks were randomly allotted into eight pens to investigate the effect of different dietary concentrations of chromium (Cr) in the form of chromium chloride, and different dosages of florfenicol on humoral immune responses by determining antibody titers to Newcastle disease (ND) vaccines using the hemagglutination inhibition test. The results indicated that ND antibody titers were significantly higher in chicks receiving Cr at low (5 mg/kg feed) and middle (10 mg/kg feed) dose compared with the control (p<0.01). However, ND antibody titers were significantly decreased in chicks receiving Cr at a high dosage of 500 mg/kg feed (p<0.01), though the ND antibody titers of the early days (d 21 and d 28 of age) were higher than that of the control group. It is suggested that excessive Cr intake has detrimental effects on ND antibody production in chicks. No significantly lower response was measured in chicks that received florfenicol at a low dosage of 50 mg/kg feed (p>0.05), but the ND antibody titers were significantly decreased in chicks receiving 200 and 400 mg/kg feed of the drug (p<0.01). The ND antibody titers of group receiving 200 mg/kg feed of florfenicol plus 10 mg/kg Cr were slightly higher than that of the group receiving single florfenicol of 200 mg/kg although, no significant differences were observed between these two treatments. It is suggested that the humoral immune response impaired by florfenicol (200 mg/kg feed) could not be significantly reversed by Cr (10 mg/kg feed).

• 대한수의학회지
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• 제31권2호
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• pp.217-222
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• 1991

Most progesterone enzyme immunoassays(EIA) are used liquid phase double-antibody separation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of there several problems, we were prompted to develop an effective EIA system by the use of higher titer of progesterone antiserum free of anti-bovine serum albumin antibodies (anti-BSA). The results obtained were as follows. 1. The antibody of progesterone antiserum was high as $1.5{\times}10^5$. 2. Percent activity bound of progesterone antiserum was about 77 at a dilution to $5{\times}10^3$ times. 3. Progesterone antiserum was contained a large amount of anti-BSA antibodies. 4. The anti-BSA was completely absorbed by using of polymerised BSA. 5. The molecular weight of albumin polymer (polymerised BSA) obtained by using 2.5% glut. araldehyde was $5{\times}10^5$.

• KSBB Journal
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• 제10권3호
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• pp.323-334
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• 1995

Hybridoma 세포의 세포 성장 속도, monoclo nal antibody의 생산성 및 세포 대사에 미치는 glucose의 영향이 조사되었다. IgG2,를 생산하는 m mouse-mouse hybridona VIII H-8 세포가 모델로 이용되었다. Glucose 농도에 따른 바성장속도의 변화는 기질저해형식 (substrate inhibition type) 의 성장모델로 나타낼 수 있었다. 초기 glucose 농도 4g/f까지는 최대 세포밀도의 증가를 보여 주었다. Glucose는 세포사망속도에 큰 영향을 나타내었고 glucose 농도와 비사망속도 간에는 반비례의 관계가 성립됨을 보였다. Glucose 농도가 증가될수록 세포의 생존율과 monoclonal an tibody의 생산이 증가되었다. Glucose 놓도가 증가될수록 glucose의 비소비속도가 증가되 었고, 초기 glucose 농도의 증가는 lactate의 총괄 비 생산속도의 증대를 가져왔다. 암모늄 이온의 총괄비 생산속도는 초기 Glucose의 농도에 의존하 였지만 암모늄 이온의 전반적인 생성속도는 초기 glucose 농도에 거 의 무관함을 보여 주었다.

• Journal of Microbiology and Biotechnology
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• 제31권3호
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• pp.349-357
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• 2021

Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in sufficient quantities for commercial use, it is necessary to raise the output by scaling up the production processes. This review describes recent trends in high-density cell culture systems established for monoclonal antibody production that are excellent methods to scale up from the lab-scale cell culture. Among the reactors, hollow fiber bioreactors contribute to a major part of high-density cell culture as they can provide a tremendous amount of surface area in a small volume for cell growth. As an alternative to hollow fiber reactors, a novel disposable bioreactor has been developed, which consists of a polymer-based supermacroporous material, cryogel, as a matrix for cell growth. Packed bed systems and disposable wave bioreactors have also been introduced for high cell density culture. These developments in high-density cell culture systems have led to the monoclonal antibody production in an economically favourable manner and made monoclonal antibodies one of the dominant therapeutic and diagnostic proteins in biopharmaceutical industry.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권2호
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• pp.130-135
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• 2000

The characteristics of two different modes of perfusion culture, intermittent and continuous bleedings, were investigated by culturing the hybridoma cells producing von Willebrand Factor (vWF) monoclonal antibody (McAb) in a 15 L bioreactor without clogging the filter. Both culture methods exhibited similar profiles of cell density and metabolite concentrations during the culture period at the cell concentration of around 1${\times}$107 cells/mL. When the perfusion rate was increased, the intermittrnt bleeding culture showed problems of ammonia accumulation and decrease of cell viability. The continuous bleeding culture in terms of nutrient consumption and metabolite production kinetics. But the analysis of specific oxygen consumption rate showed that the specific oxygen consumption rate of intermittent bleeding culture was similar to that of exponential growth phase. The continuous bleeding culture showed higher specific oxygen consumption rate of intermittent bleeding culture. finally we proved the possibility of long-term operation of continuous bleeding culture and produced approximately 40 g of vWF McAb in a 15L bioreactor after one-month operation.