• 한국식품위생안전성학회지
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• 제11권2호
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• pp.129-132
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• 1996

Recently, as concern about the residual antibiotics in milk increase, the detection methods of residual antibiotics used extensevely at the present time were investigated and compared to their properties and the detection limits of variable antibiotics. At first, comparactive tests of the detectable sensitivity of 4 test organisms, B. cereus, B. subtilis, M.luteus, B.stearothermophilus C-953, were performed by disc assay. As a result, B.stearothermophilus was the most sensitive strain of all other strains and showe the detect limit of 5-50 ppb for penlicillins (PCs). And also, B.subitilis was showed the more effective detection limit, 200-400 ppb, for aminoglycosides (AGs) and M.luteus was showed predominant sensitivity , 50-500 ppb for macrolides(MLs) and B.cereus was the most sensitive strain for tetracyclines (TCs) and showed the detection limit of 100-400 ppb. Therefore, each test strains were showed a different sensitivity in the detection of the different antibiotic families. When the detection limit of disc assay and other methods were compared, TTCmethod was less sensitive than other methods showing 5-50 ppb detectable lebel for PCs. Also, for the detection of other antibiotic families TTC method was showed the worst sensitivity and Delvo and Charm Farm tests were similar to the detectable properties of AGs and MLs. Although disc assay was showed the similar detection limit for PCs with Delvo and Charm Farm, it was more widely effective for the detection of kanamycin, erythromycin, chlortetracycline, doxycycline, verginiamycin and so on than Delvo or Charm Farm. CharmII test was showed the best sensitivity for the most of antibiotics except neomycin and gentamycin. But it was necessary that different tests must be performed to each antibiotic family and so it was regarded that the effectiveness of that method was low.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제49권4호
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• pp.198-207
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• 2023

Objectives: This study investigated causative strains and their antibiotic sensitivity in patients who were hospitalized for maxillofacial odontogenic infections at a tertiary center in South Korea over the past 10 years with the aim of providing guidelines for the selection of appropriate empirical antibiotics. Materials and Methods: Patients with head and neck fascial space abscesses due to odontogenic infections who underwent incision and drainage surgery with pus culture tests between 2013 and 2022 at the Department of Oral and Maxillofacial Surgery, Dankook University Hospital were included. The bacterial isolates and antibiotic sensitivity of each strain were analyzed for 2013-2022, 2013-2017, and 2018-2022. The affected fascial spaces were classified into primary, secondary, and deep neck spaces. Results: In the 192 patients included in this study, 302 strains were detected. Viridans streptococcus had the highest frequency (51.7%), followed by Prevotella spp. (16.9%), Staphylococcus spp. (5.6%), and Klebsiella pneumoniae (4.6%). The identification rate of viridans streptococcus significantly increased from 41.8% in 2013-2017 to 60.9% in 2018-2022. Viridans streptococcus showed an antibiotic sensitivity of 80.5% to ampicillin; the sensitivity to penicillin antibiotics decreased over the study period. Antibiotic susceptibility was approximately 94% for third-generation cephalosporins. K. pneumoniae, which was identified at a high percentage in patients with deep neck space infection, showed increasing antibiotic resistance to most antibiotics over the study period. Conclusion: Viridans streptococcus was identified in head and neck fascial space abscesses with the highest frequency. Empirical antibiotics should be effective against this strain; penicillin antibiotics are considered inappropriate. For effective treatment of deep neck space abscesses, bacterial culture and antibiotic sensitivity tests performed as soon as possible are essential.

• 대한족부족관절학회지
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• 제13권2호
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• pp.146-149
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• 2009

Purpose: Foot infections are common complications in patients with diabetes. The patients are usually immune-compromised; therefore the pathogens could be resistant to narrow spectrum antibiotics. Those drugs, however, are categorized as specially managed antibiotics, and access are difficult without confirming of the pathogens. Our aim was to analyze the common pathogens in diabetic foot infection and figure out the proper antibiotics. Materials and Methods: We studied 68 patients treated with diabetic foot infection. The pathogens which caused the infection and their sensitivity to initial antibiotics were analyzed. We also investigated the change of the antibiotics after the confirming of the culture result and average time to get the result. Results: Among the 68 patients, 56 (82%) received cephalosporin and beta-lactam antibiotics. Only 12 (18%) who were confirmed the drug resistant pathogens from previous culture, were treated with broad spectrum antibiotics such as vancomycin and tazoperan. Average culture study time was 6 days. Methicillin-resistant staphylococcus aureus (MRSA) was cultured in 19 patients (28%), Methicillin-resistant coagulase negative staphylococcus (MRCNS) in 11 patietns (17%), pseudomonas in 11 patients (17%). Total 44 (65%) including 3 of other antibiotics resistant pathogen needed broad spectrum antibiotics. Thirty two patients (47%) were resistant to initial antibiotics.irt follow up culture, 2 MRSA and 2 MRCNS were found. The antibiotics resistant pathogens were confirmed in 48 (71%) patients at last. Conclusion: Diabetic patients with foot infection need proper antibiotics from initial treatment. The proper broad spectrum antibiotics should assigned to the patients from the first time without the confirming of the culture results.

• 한국환경보건학회지
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• 제1권1호
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• pp.46-51
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• 1974

The agar plate titrations of antibiotic sensitivities of Salmonella and Shigella isolated from human during 1967 to 1972 were studies. 1. The most effective antibiotics against Salmonella and Shigella were chloramphenicol, tetracycline, kanamycin, minomycin, and gentamycin. 2. All strain of Salmonella typhi were resistant to cloxacilline. 3. The most effective antibiotics against Shigella were kanamycin, gentamycin and minomycin.

• 한국동물위생학회지
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• 제22권1호
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• pp.93-101
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• 1999

This test process on screening method for the detection of residual antibiotics in milk is simple, economic, sensitive to residual antibiotics and was given approval international organs. Thus, this study was carried out the comparison of Disk assay method and TTC-II method for sensitivity and minimum detectable range of antibiotics in raw milk. The results of this study was summarized as follows ; 1. The number of samples requested for treatment of mastitis was 198 samples. Comparison or analytical results among the methods of TTC-II, disk assay and Delve sp was that TTC-II 37 samples(18.6% ), Disk assay 125samples(63.1%), Delve SP 130 samples(65.7% ) reacted positively. Conformity rate of Delve SP and Disk assay was 70%. 2. Detectable limits of disk assay method in some antibiotics were more sensitive than those of official method(0.05-0.0025ppm in the $\beta$-lactams, 1ppm in two aminoglycoside, 0.2 ppm in one tetracycline, similar in one macrolide) 3. For sensitivity of residual sulfonamides TTC-II was much more sensitive than disk assay. Detectable limits of sulfamethazine and sulfadimethoxine were 30 to 50ppm levels. 4. The best medium preservation period is 1-2 days. 5. Concentration of brome cresol purple related to resistance for B stearothermophilus culture was 24ppm/ml. These results show that disk assay method for screening detection of antibiotics residuces in milk is worthy of use.

• 한국미생물·생명공학회지
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• 제36권4호
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• pp.360-365
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• 2008

식품 내의 잔류 항생물질의 검사는 주로 미생물의 생육 억제 여부를 통한 생물학적인 분석을 이용한다. 이러한 방법은 여러 가지 계열의 항생제들에 대한 미생물의 민감성을 토대로 이용되고 있다. 그러나 현재 사용하고 있는 미생물들로는 동물용의약품으로 허가되어 식용동물에 사용되고 있는 점점 다양해지는 항생제 및 합성항균제를 식품에서 모두 검출할 수는 없다는 한계가 있다. 그러므로 본 연구는 검출 가능한 항생제 및 합성항균제 계열의 범위를 확대하고, 각 약품별 감도를 증진시키기 위한 새로운 방법들을 조사하였다. B. megaterium ATCC 9885, B. subtilis ATCC 6633, B. cereus ATCC l1778 and Geobacillus stearothermophilus (B. stearothermophilus) ATCC 10149를 사용한 검사의 민감성은 macrolides, quinolones와 monensin, chloramphenicol에 대한 검출 감도가 낮았다. 반면에 M. luteus(K. rhizophila) ATCC 9341는 macrolides에 대한 높은 검출 감도를 나타냈고, E. coli ATCC l1303는 quinolones와 aminoglycosides에 대한 높은 민감성을 나타냈다. 결론적으로, 두 균주의 추가로 검출가능한 항생제 및 합성항균제의 범위를 확대하였으며, 검출감도를 높일 수 있게 되었다.

• Archives of Plastic Surgery
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• 제34권3호
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• pp.314-318
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• 2007

Purpose: Pressure sore wound develops inevitably in long-term, immobilized and hospitalized patients. Sore wound infection is common problem and makes healing process difficult. We aimed to identify the pathogens of the purulent discharge in sore wound and to obtain information for appropriate antibiotics through a sensitivity test Methods: The bacteriologic study was made on 120 cases of patients who admitted or visited our hospital from 2004 January to 2005 December for sore wound treatment. Culture material was collected in BBL transport media with cotton swab and cultured by MacConkey agar plate. The method of MIC by VITEK and Microscan was used for sensitivity test. Results: Among 120 specimens, organisms were isolated from 77(64.2%) cases. Gram positive organisms were cultured in 73 specimens, Gram negative organisms in 46 specemens, and fungi in 2 specimens. Mixed infection by Gram (+) and Gram (-) bacteria were observed in 34 specimens. Among them, S. aureus was the most common isolate in 24(31.2%) patients and 10 (13.0%) S. Aureus isolates were MRSA. The most prevalent Gram-negative organism was Escherichia coli in 20 patients(25.9%). Vancomycin and teicoplanin showed highest sensitivity to Gram-positive organisms and imipenem and amikacin to Gram-negative organisms. Conclusion: Pressure sore wound demands consideration of multimodal therapeutic aspects and these findings would be useful informations to physicians, nurses and clinical assistants in understanding the nature of sore wound and selecting appropriate antibiotics.

• 한국가축번식학회지
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• 제9권1호
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• pp.66-71
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• 1985

After the induction of estrus by the PGFF2$\alpha$-GTH combination on the 118 repeat breeder cow during of 6-13 days of the estrus cycle, the effects of GTH, antibiotics, antibiotics-GTH combination were compared. The results of this study, the following conclusion were obtained: 1. The rate of estrus was reduced the administration of GTH before the administration of PGF2$\alpha$. 2. The rate of ovulation delayed was 24.6% in the group with the administration GTH, but 34.1% without the administration GTH. Duration of ovulation was shortened by GTH. 3. The impregnation induction by the administration of PGF2$\alpha$, PGF2$\alpha$-antibiotics, PGF2$\alpha$-GTH-antibiotics were 41.7%, 69.5%, 55.6% and 80.8% respectively injection in the uterus was more effective than the administration. Before or after the A.I., the above combination was most effective. 4. In antibiotics sensitivity test in vitro of the intravagina bacteria, 5.0%, 12.7% and 60.0% of bacteria were sensitive to Penicilline, Streptomycin and Gentamicin respectively.

• 대한미생물학회지
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• 제22권4호
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• pp.413-425
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• 1987

In the present experiments, isolated Vibrio species from marine and clinical specimens from July, 1985 to October, 1986, had the results as follows: 1. The 55 strains of Vibrio were isolated and identified; Vibrio parahaemolyticus was 35 strains, Vibrio vulnificus was 10 strains, Vibrio alginolyticus was 10 strains. 2. In the K-serotyping of Vibrio parahaemolyticus, fourteen serotypes identified but three were not strains typable by the availble K-antisera. 3. In the Kanagawa phenomenon experiment of Vibrio parahaemolyticus, it proved positive reaction, 14 of 15 strains(93%) isolated from the patient and 13 of 20 strains(65%) isolated from the nature. 4. In twelve antibiotic resistance experiments, Vibrio parahaemolyticus and Vibrio alginolyticus showed 100% resistance on ampicilline, but Vibrio vulnificus showed 100% sensitivity. But all of them proved 100% sensitivity on chloramphenicol, tetracycline, nalidixic acid. 5. In the antibiotic resistance patterns, Vibrio parahaemolyticus proved that 15 strains(43%) resisted on 4 antibiotics and 5 strains(14%) resisted on 7 antibiotisc and. Vibrio vulnificus proved that 1 strain(10%) resisted on 2 antibiotics and 6 strains(60%) without resistance, Vibrio alginolyticus proved that 7 strains(70%) resisted on 3 antibiotics and 2 strains(20%) resisted on 8 antibiotics.

• 한국환경보건학회지
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• 제16권2호
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• pp.127-133
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• 1990

The purpose of the study was to identify the characteristics of the pathogenic microorganism Actinobacillus actinorayceteracomitans. The experiments of this study consist of the decision of existence or nonexistence of Actinobacillus actinorayceterncoraitans in the air of dental clinics, the effect of sugar on the growth of Actinobacillus actinorayceteracoraitans, and the sensitivity of Actinobacillus actinoraycetemcoraitans to Fluorine and antibiotics. The results of the experiments are as follows. 1. Actinobaeillus actinornyceteracornitans did not exist in the air of 4 dental clinics in Seoul 2. The growth of Actinobacillus actinornycetemcornitans in the media containing glucose or sucrose was faster than in the media without these, and glucose was more effective than sucrose. 3. Actinobacillus actinomyceteracoraitans was not susceptible to Fluorine at concentration between 0 ppm and 300 ppm. 4. Order of sensitivity of Actinobacillus actinomycetemcomitans to antobiotics was as follows: Tetracycline > Kanamycin > Ampicillin.