• 생약학회지
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• 제39권2호
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• pp.118-122
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• 2008

This study was conducted to evaluate the inhibitory effects of betulinic acid isolated from Ziziphus jujuba on various human rotavirus, such as KU, S2 and YO. The results obtained are summarized as follows: At the concentration of betulinic acid 0.1/2%, all human rotavirus is showed a maximum effect on their growth even though it's evaluated on the in vitro test. The each inhibitory rate of MA-104 cells infected by human rotavirus KU, S2 and YO was 62.1%, 59.7% and 65.2%, respectively, at the concentration of 0.1/2%. The anti-virus activity of betulinic acid was showed as the dose-dependent manner at the used dosages except at the dose of 0.1%.

• 대한의생명과학회지
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• 제12권3호
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• pp.209-215
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• 2006

The mutability and frequency of genetic reassortment characteristic of rotavirus and resultant antigenic changes make the rotavirus formidable challenges for control efforts such as the vaccine development. An alternative approach to overcome these difficulties in development of the rotavirus vaccine is to develop effective inhibitors of the virus infection. As an effort to achieve this, effects of glycyrrhetinic acid (GA), which is an active component of glycyrrhizin, on MA-14 cell infection were examined by employing the human rotavirus isolated from Korea, K-21. The data obtained showed that MA-104 cell infection of the K-21 rotavirus was greatly influenced by the presence of both $18{\alpha}-Ga\;and\;18{\beta}-GA$. Both types of GA have inhibited more than 60% of the rotaviral infection at the concentration of 7.68mM. This inhibition effect became much more evident at the higher concentrations of GA. However, the type of GA did not make much differences on the inhibition effect of the drug. Although GA has to be used in high concentrations to exhibit anti-viral activity and to be virostatic, a long history of safe and high dose usage of licoriece in clinical settings in the Far East makes the GA as an attractive inhibitor of the rotaviral infection.

• Archives of Pharmacal Research
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• 제23권2호
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• pp.172-173
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• 2000

The relationship between the metabolites of glycyrrhizin (18$\beta$-glycyrrhetinic acid-3-O--D-glu-curonopyranosyl-($1{\rightarrow}2$)-$\beta$-D-glucuronide, CL) and their biological activities was investigated. By human intestinal microflora, CL was metabolized to 18$\beta$-glycyrrhetinic acid (GA) as a main product and to 18$\beta$-glycyrrhetinic acid-3-O-$\beta$-D-glucuronide (GAMG) as a minor product. The former reaction was catalyzed by Eubacterium L-8 and the latter was by Streptococcus LJ-22. Among GL and its metabolites, GA and GAMG had more potent in vitro anti-platelet aggregation activity than GL. GA also showed the most potent cytotoxicity against tumor cell lines and the potent inhibitory activity on rotavirus infection as well as growth of Helicobacter pylori. GAMG, the minor metabolite of GL, was the sweetest.

• 한국축산식품학회지
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• 제31권1호
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• pp.81-85
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• 2011

본 연구에서는kefir중의 polysaccharide분획이 HRV의 MA-104세포 감염에 미치는 억제효과를 조사하였다. (1) 추출된 polysaccharide는 DEAE 이온교환 chromatography에서 fraction I의 미결합 분획과 fraction II, III, IV의 결합 분획으로 나뉘어졌다. (2) Polysaccharide fraction II, III, IV는 각각 4.8, 5.3, 1.4 mg/mL의 농도로 첨가했을 때 HRV의 MA-104 세포감염에 대하여 약 97%, 99%, 100%의 억제효과를 나타냈다. 본 연구의 결과를 종합하면 kefir의 다당체가 유아설사변에서 분리한 rotavirus S-2가 MA-104 cell 감염에 97% 이상 억제효과가 있어 동절기 유아의 바이러스성 급성설사 예방에 kefir를 급식하는 것이 바람직하다고 사료된다.

• Journal of Microbiology and Biotechnology
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• 제31권8호
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• pp.1175-1182
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• 2021

We investigated the effect of bovine lactoferricin (Lfcin-B), a peptide derived from bovine lactoferrin, on activation of intestinal epithelial cells in IEC-6 intestinal cell, and protection against in vivo rotavirus (RV) infection. Treatment with Lfcin-B significantly enhanced the growth of IEC-6 cells and increased their capacity for attachment and spreading in culture plates. Also, Lfcin-B synergistically augmented the binding of IEC-6 cells to laminin, a component of the extracellular matrix (ECM). In the analysis of the intracellular mechanism related to Lfcin-B-induced activation of IEC-6 cells, this peptide upregulated tyrosine-dependent phosphorylation of focal adhesion kinase (FAK) and paxillin, which are intracellular proteins associated with cell adhesion, spreading, and signal transduction during cell activation. An experiment using synthetic peptides with various sequences of amino acids revealed that a sequence of 9 amino acids (FKCRRWQWR) corresponding to 17-25 of the N-terminus of Lfcin-B is responsible for the epithelial cell activation. In an in vivo experiment, treatment with Lfcin-B one day before RV infection effectively prevented RV-induced diarrhea and significantly reduced RV titers in the bowels of infected mice. These results suggest that Lfcin-B plays meaningful roles in the maintenance and repair of intestinal mucosal tissues, as well as in protecting against intestinal infection by RV. Collectively, Lfcin-B is a promising candidate with potential applications in drugs or functional foods beneficial for intestinal health and mucosal immunity.

• KSBB Journal
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• 제26권1호
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• pp.62-68
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• 2011

To isolate Lactic acid bacteria for animals, we have screened from Kim-chi, swine intestine, swine feces, and dairy products by random selection and anti-viral, antipathogenic bacteria test. Among them, CLP-1 shown that inhibitory effect against rotavirus, porcine epidemic diarrhea (PED) virus, Salmonella sp, and E.coli. By examining biological property, API-ZYM and identified Lactobacillus plantarum by 16S rDNAgene sequence. CLP-1 determined resistance to low pH and bile salt. Futhermore, the cell body of CLP-1 adhered to the intestinal epithelium tissue of swine and Caco-2 cell. CLP-1 was examined on cell immune system modulating activity in vitro. The whole cell and cell culture supernatant was increasing of interferon-${\beta}$ activity. And then, CLP-1 increased prevention effect by Salmonella enteritidis infection in SPF chickens. And we determined similar result in pigs.

• Journal of Animal Science and Technology
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• 제44권6호
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• pp.809-816
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• 2002

키틴의 탈아세틸화로 얻어지는 화합물인 키토산과 키토올리고당은 다양한 생리활성 기능을 나타내므로 생물의학적인 응용에 많은 관심이 집중되고 있다. 본 연구는 키토올리고당과 수용성 키토산의 열처리 후 올리고당 함량의 변화와 어린이 바이러스성 설사를 유발하는 HRV(human rotavirus)의 MA-104 세포감염에 억제작용을 나타내는지 확인하기 위하여 수행하였다. 그 결과 열처리 후 비색법으로 측정한 올리고당의 함량은 수용성키토산의 경우 62.67 %에서 60.45%로 약 2%정도, 키토올리고당은 59.48%에서 54.31%로 약 5%정도 감소하였다. 키토산 유래물질의 항로타바이러스성 탐색은 AEC staining으로 측정하였으며, 수용성키토산은 농도 0.125% 이상에서 HRV S2의 세포감염에 있어 90% 이상의 감염 억제효과가 있었으며, HRV Wa의 경우 89% 이상의 억제효과가 있었다. 키토올리고당은 HRV의 세포감염에 억제효과가 없었다. 열처리한 시료의 경우, 수용성 키토산과 키토올리고당 모두 열처리가 HRV의 감염 억제에 영향을 미치지 못하는 것으로 나타났다. 따라서 중합도가 높은 수용성키토산은 열처리를 거치는 식품에 첨가하여도 그 기능이 크게 감소하지 않으므로 향후 식품첨가물로서 이용가능성이 클 것으로 사료된다.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.273-279
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• 2012

Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The $IC_{50}$ value of hesperidin was determined to be 152.3 ${\mu}M$ in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 ${\mu}M$) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-$G_1$ population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-$_{xl}$ in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma.