• Journal of Pharmaceutical Investigation
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• v.36 no.5
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• pp.309-314
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• 2006

The multicellular layers(MCL) of human cancer cells is a three dimensional(3D) in vitro model for human solid tumors which has been used primarily for the assessment of avascular penetration of anti-cancer drugs. For anti-cancer drugs with penetration problem, MCL represents a good experimental model that can provide clinically relevant data. Calcein-AM is a fluorescent dye that demonstrates the cellular vitality in a graded manner in cancer cell culture system. In the present study, we evaluated the use of calcein-AM for determination of anti-proliferative activity of anti-cancer agents in MCL model of DLD-1 human colorectal cancer cells. Optical sectioning of confocal imaging was compromised with photonic attenuation and penetration barrier in the deep layers of MCL. By contrast, fluorescent measurement on the cryo-sections provided a feasible alternative. Cold pre-incubation did not enhance the calcein-AM distribution to a significant degree in MCL of DLD-1 cells. However, the simultaneous determination of drug penetration and cellular vitality appeared to be possible in drug treated MCL. In conclusion, these data suggest that calcein-AM can be used for the simultaneous determination of drug-induced anti-proliferative effect and drug penetration in MCL model.

• Advances in concrete construction
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• v.16 no.2
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• pp.119-126
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• 2023

Steel fiber reinforced self-compacting concrete (SFRSCC) has good workability such as high flowability and good cohesiveness. The workability, compressive strength, splitting tensile strength, and anti-penetration characteristics of three kinds of SFRSCC were investigated in this paper. The fraction of steel fibers of the SFRSCC is 0.5%, 1.5% and 2.0% respectively. The results of the static tests show that the splitting tensile strength increases with the increase of fraction of steel fibers, while the compressive strength of 1.5% SFRSCC is lowest. It is demonstrated that the anti-penetration ability of 1.5% SFRSCC subjected to a velocity projectile (200-500 m/s) is better than 0.5% and 2.0% SFRSCC according to the experimental results. Considering the steel fiber effects, the existing formula is revised to predict penetration depth, and it is revealed that the revised predicted depth of penetration is in good agreement with the experimental results. The conclusion of this paper is helpful to the experimental investigations and engineering application.

• Journal of Pharmaceutical Investigation
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• v.36 no.6
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• pp.401-403
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• 2006

Topical preparations such as cream for Acyclovir(ACV), a potent anti-viral agent for the treatment of herpes simplex and herpes zoster, have been marketed in the world since 1993. However, the skin penetration rate of ACV from generic cream formulations sold in Europe has been found to be lower than the original $Zovirax^{\circledR}$ cream. In this study, we formulated ACV into a novel organogel system and compared the skin penetration characteristics with $Zovirax^{\circledR}$ cream. The rate and amount of skin penetration of ACV from the organogels were 6.3-fold greater than those obtained with $Zovirax^{\circledR}$ at an ACV concentration of 5%. The solubilizing effect of oil phase and anti-nucleation effect exhibited by sodium alginate contained in water phase are most likely attributed to enhanced ACV skin penetration property.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.25 no.1
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• pp.82-88
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• 2015

Objectives: This study is designed to analyze the penetration performance into ceiling materials containing asbestos of scattering prevention agents and investigate the change in penetration depth and viscosity according to the dilution rate of anti-scattering agents diluted with distilled water. Methods: Five different types of scattering prevention agents were spread on plate-type asbestos ceiling materials. The penetration depth of each coated ceiling material was measured by energy dispersive spectroscopy (EDS) analysis, based on X-ray fluorescence (XRF) results of the non-coated ceiling materials. Test equipment installed the ceiling materials and 60 minutes were collected at a flow rate of $10{\ell}/min$ at a filter of 25 mm. Results: An EDS analysis of the cross-section of ceiling materials constructed with a scattering prevention agent revealed that potassium is detected in the process of penetrating hardener solidification and this element could be an indicator for infiltration. When anti-scattering agents with different viscosities were constructed and the penetration depth was analyzed by potassium detection assessment using EDS, the depth results with viscosities of 5.0, 2.5, and 1.9 cP were 98.5, 103, and $147{\mu}m$, respectively. Penetration performance improved with decrease in viscosity. Conclusions: For asbestos ceiling materials, it is concluded that a higher dilution rate of the scattering prevention agent leads to lower viscosity, and hence a deeper penetration depth from $156{\mu}m$ to 3 mm. The asbestos anti-scattering properties according to the penetration depth will be confirmed through further study.

• Structural Engineering and Mechanics
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• v.28 no.6
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• pp.715-725
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• 2008

New types of armor, including space armor, multiple-layered armor, composite armor and modular armor have been successfully developed and installed on the armored vehicles of several nations. The protective capability of armor against penetration is established. Of developed composite armor, space armor has a simple structure and is easy to produce and can be produced at low cost. This study uses the finite element package DYTRAN and the pre and post processor PNTRAN to elucidate the ballistic resistance and penetration of space armor. Factors such as armor thickness, space between armors and projectile profile are considered. A technique for simulating the protection afforded by armor and supporting the design of space armor is developed.

• International Journal of Highway Engineering
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• v.13 no.1
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• pp.21-32
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• 2011

The frost penetration depth of pavement is usually estimated from the freezing index that made temperature data analysis of 30 years and decided the thickness of anti-frost layer. The field monitoring region in study was divided into five regions by freezing index 550~650$^{\circ}C{\cdot}$day, 450~550$^{\circ}C{\cdot}$day and 350~450$^{\circ}C{\cdot}$day. Each region has three-section of road pavement such as cutting area, boundary area of cutting and banking, and lower area of banking. The field monitoring system was established both in the section of anti-frost layer and in the section without anti-frost layer. The data analysis was conducted for determination of frost penetration depth within the paved road by the field monitoring system. The result showed that The temperature of subgrade without anti-frost layer shows below zero in centigrade for the region of freezing index 550~650$^{\circ}C{\cdot}$day, up and down around zero degree in subgrade for the region of freezing index 450~550$^{\circ}C{\cdot}$day, and there is no place existed below zero degree in subgrade for the region of freezing index below 450$^{\circ}C{\cdot}$day. With comparison of field frost penetration depth for the cross-sections of pavement, the cutting area shows the greatest frost penetration depth, and less influence of frost penetration depth for the boundary area of cutting and banking, and the least influenced for the lower area of banking.

• Journal of Pharmaceutical Investigation
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• v.36 no.6
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• pp.385-392
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• 2006

Paclitaxel is an important chemotherapeutic agent for the treatment of human solid tumors. Multicellular resistance(MCR) is considered to be a major mechanism of resistance of human solid tumors to chemotherapeutic agent such as paclitaxel, which includes barriers to drug penetration through tumor tissues. Multicellular layers(MCL) cultures resemble in vivo tumor condition in terms of MCR and has been used successfully to produce clinically relevant data. In the present study, we evaluated the penetration characteristics and post-penetration anti-proliferative activity of paclitaxel using MCL of human colorectal cancer cells(DLD-1 and HT-29) grown in Transwell inserts. The penetration of $[^{14}C]-paclitaxel$ was slower than that of mannitol which penetrates via paracellular pathway in DLD-1 MCL. The penetration of $[^{14}C]-paclitaxel$ was faster in HT-29 MCL compared to DLD-1 MCL, i.e., at 10 ${\mu}M$ 100% and 40% penetration were observed after 48 hr incubation for HT-29 and DLD-1 cells, respectively. When calculated using anti-proliferative activity in the conditioned media of bottom chamber, the penetration after 24 hr was very limited(less than 50%) and concentration-dependent at the concentrations tested in both MCL's. These results suggest that limited and differential penetration of paclitaxel in tumor tissues may contribute to lower and differential efficacy against human solid tumors.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.4
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• pp.141-148
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• 2002

Repair were made for front pillar, center pillar and side-step panel for lightweight vehicles with head-on and 40% off-set collision of 15 km/h in a RCAR standard. The salt dilution was sprayed and the compression tests were performed for vehicles with and without anti-corrosional treatment after repair. After 764 hours of salt-dilt sprayed test without using anti-corrosion, the mean penetration depth fur corrosion was shown to be 58% of the thickness. The resulyed decrease in bending stiffness by 10∼20% can cause reduction of the residual life and crash-absorption capability for damaged vehicles. The corrosoin safety tests showed that the anti-corrosional treatment should be made to improve the safety characteristics for a or damaged car.

• Journal of the Korea Institute of Military Science and Technology
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• v.13 no.4
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• pp.520-527
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• 2010

The kill probability of a missile depends on guidance error, warhead performance, and etc. In this paper, we analyzed the kill probability of anti-tank missile in a new approach. Under the condition that the missile hit the target, we studied the effect of angle of attack and impact angle. High impact angle increase the probability that the missile hits the upper armour which is relatively weaker, while high angle of attack at the impact instant decreases the effectiveness of the jet induced by the warhead. We proposed a way to increase the capability of penetration by analyzing the interrelation between impact angle and angle of attack.

• Biomolecules & Therapeutics
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• v.14 no.2
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• pp.90-95
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• 2006

CKD-602 exerts its antitumor effect via inhibition of topoisomerase I in cancer cells. Multicellular spheroid (MCS) and Multicellular layers (MCLs) are known as in vitro 3-dimensional models which closely represent tumor conditions in vivo. In order to investigate the potential of CKD-602 against human colorectal tumors, we evaluated the anti-proliferative activity and penetration ability of CKD-602 in MCS and MCL cultures of DLD-l human colorectal cancer cells, respectively. The maximum effects($E_{max}$) induced by CKD-602 were significantly lower in MCS compared to monolayers (48% vs 92%). With prolonged drug exposure, the $IC_{50's}$ of CKD-602 decreased to $23.5{\pm}1.0nM$ in monolayers after 24 h exposure and $42.3{\pm}1.7nM$ in MCS after 6 days, respectively. However, no further increase in effect was observed for exposure time longer than growth doubling time (Td) in both cultures. Activity of CKD-602 was significantly reduced after penetration through MCL and also with cell-free insert membrane. In conclusion, CKD-602 showed significantly decreased anti-proliferative activity in 3D cultures (MCS) of human colorectal cancer cells. Tumor penetration of CKD-602 could not be determined due to loss of activity after penetration through cell free insert membrane, which warrants further evaluation using a modified model.