• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.405-412
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• 2022

Echinodorus cordifolius (L.)은 택사과(Alismataceae)에 속하는 수생 식물이다. Echinodorus cordifolius (L.)의 피부 항노화 활성에 대한 연구는 아직까지 보고되지 않았다. 따라서 본 연구의 목적은 Echinodorus cordifolius (L.)로부터 70% 에탄올 추출물 (ECEE)을 제조하고 항산화 및 hyaluronidase 억제 활성을 통해 피부 항노화 기능성 소재로서의 가능성을 확인하였다. ECEE는 EC₅₀과 IC₅₀ 값이 각각 31.4, 300, 450 ㎍/mL로 우수한 DPPH, hydrogen peroxide 소거 활성 및 hyaluronidase 억제 활성을 가지는 것으로 나타났다. ECEE는 불멸화된 인간 각질세포인 HaCaT 세포에서 1 mM H₂O₂로 유도된 산화적 스트레스에 대해 농도 의존적으로 세포 보호 효과 증가 및 세포 내 활성산소종을 생성을 억제하는 것으로 나타났다. 또한 ECEE는 hyaluronic acid (HA) 합성 효소인 hyaluronan synthase 2 발현 수준을 상향 조절하고 HA 분해 효소인 hyaluronidase 2(HYAL2)의 발현 수준을 하향 조절하여 HaCaT 세포에서 HA 생성량을 증가시켰다. 따라서 이러한 결과를 종합하면 ECEE는 항산화, hyaluronidase 억제 및 HA 생성 촉진의 우수한 잠재력을 가진다는 것을 확인하였고 이를 통해 피부 노화를 방지하기 위한 천연 기능성 화장품 소재로서의 충분한 활용 가치가 있다고 판단된다.

• 대한화장품학회지
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• 제48권3호
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• pp.275-285
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• 2022

본 연구에서는 감(Diospyros kaki)의 1,3-butylene glycol 추출물(DBG)과 에탄올 추출물(DET)를 이용하여 항산화, 항노화 및 미백 기능을 규명하였다. 항산화능은 DPPH와 superoxide dismutase (SOD) assay를 통해 규명하였으며, 그 결과 DBG와 DET 모두 현저한 DPPH 소거능 및 SOD-유사능을 보여주었다. 또한 elastase와 hyaluronidase 억제능 그리고 matrix metalloproteinase (MMP-1) 발현을 통해 항노화 기능을 규명하였다. DBG와 DET 모두 농도의존적으로 elastase와 hyaluronidase를 억제하였으며, MMP-1 발현 역시 저해하였다. 마지막으로 미백 기능을 규명하기 위하여 tyronisinase 억제 및 melanin 생성 저해능을 알아보았는데, DBG 추출물만이 tyrosinase를 저해할 수 있었다. 요약하면, 감 추출물은 강한 항산화, 항노화, 미백 기능을 갖고 있으며, 추후 화장품 소재로 활용이 가능할 것으로 사료된다.

• Fisheries and Aquatic Sciences
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• 제23권3호
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• pp.6.1-6.9
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• 2020

Background: Halophyte plant (HPs), a salt-resistant flora, has been reported to provide several health benefits, but the knowledge of its cosmeceutical potential is still ambiguous. Here, 70% ethanol extracts of 22 HPs collected from along the coast of South Korea were investigated for their potentials of antioxidant, anti-aging, and whitening properties for use as materials in novel cosmeceuticals. Methods: Antioxidant activities were determined by DPPH (1,1-diphenyl-2-pricrylhydrazyl) free radical and hydrogen peroxide scavenging assays, and skin aging-related enzyme activities (anti-elastase, anti-collagenase, anti-hyaluronidase, and anti-tyrosinase) were evaluated by using the spectrophotometric method. Results: Among the 22 HPs, we found that Ischaemum antephoroides f. coreana and Atriplex gmelinii extracts presented the strongest scavenging effects against DPPH free radical and hydrogen peroxide, respectively. Our finding additionally suggested that Salicornia europaea extract might provide a major source of anti-elastase and anti-hyaluronidase; meanwhile, Rosa rugosa extract showed the highest anti-collagenase effect. Furthermore, the highest tyrosinase inhibitory activity was possessed by Spartina anglica extract. Conclusion: These findings may suggest that halophyte plants showing biological activities may be potent inhibitors of tyrosinase, elastase, collagenase, and hyaluronidase and could be useful for application in cosmeceuticals.

• The Korean Journal of Physiology
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• 제22권2호
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• pp.259-272
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• 1988

Type I allergic reaction and it's related clinical manifestations are known to occur by the effects of various chemical mediators. These chemical mediators are released from circulating basophils and tissue mast cells, which become 'sensitized' through the binding of antigens and antibodies of the IgE type to their cell surface receptors. Efforts to elucidate the mechanism of the release of these mediators, especially that of histamine, have been persued for years. The mechanism is not yet clarified at the present time. Recent reports of hyaluronidase, an enzyme known to be involved in the tissue inflammatory process, as possible participant in type I allergic reaction, initiated this study. Relationships between the hyaluronidase activity and histamine release from the sensitized rat peritoneal mast cells were investigated. Also anti-allergic agents, tranilast and disodium cromoglycate, along with known histamine releasers, morphine and compound 48/80, were used to observe the inhibitory and stimulatory effects of these substances on the hyaluronidase activity as well as histamine release from the rat mast cells. The results obtained are summarized as follows: 1) Hyaluronidase activity and histamine release from sensitiaed rat peritoneal mast cells started to increase on the 4th day of postsensitization. Hyaluronidase activity reached it's peak value on the 7th day of postsensitization and that of histamine release on the 14th day of postsensitization. 2) Hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells, pre-treated with tranilast revealed significant decrease in comparison with those of non-treated cells. 3) Hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells, pre-treated with tranilast, followed by morphine injection, revealed significant increase in comparison with those of tranilast treated cells. 4) In vitro study of hyaluronidase activity and histamine release from un-sensitized rat peritoneal mast cells, using morphine and compound 48/80 as activators, revealed significant increase compared to those of non-activator used cells. 5) In vitro study of hyaluronidase activity and histamine release from un-sensitized rat peritoneal mast cells, pre-treated with tranilast and disodium cromoglycate, using confound 48/80 and morphine as activators revealed significant decrease in comparison with those of tranilast and disodium cromoglycate treated cells. From above results, participation of enzyme hyaluronidase in the process of histamine release from sensitized rat pertioneal mast cells, could be suggested. It was also quite evident that the clinically used anti-allergic agents, tranilast and disodium cromoglycate, have significant inhibitory function on the hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells, while morphine significantly increased the hyaluronidase activity and histamine release from sensitized rat peritoneal mast cells.

• 약학회지
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• 제36권2호
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• pp.140-149
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• 1992

Anti-allergic action of each water extracts of some crude drugs was investigated in mice and rats. The activity of hyaluronidase which was used in the screening test of anti-allergic action was inhibited significantly by Amomi Semen, Asiasari Radix, Cimicifugae Rhizoma, Cinnamomi Ramulus, Glycyrrhizae Radix and Scutellariae Radix. The 48-hour homologous passive cutaneous anaphylaxis(48-hr PCA) in mouse ear was inhibited significantly by intraperitoneal(i.p.) injection of Amomi Semen, Cimicifugae Rhizoma, and ketotifen, a comparative drug of an anti-allergic action. The increase of vascular permeability induced by histamine or serotonin was inhibited significantly by i.p. injection of Amomi Semen, Cimicifugae Rhizoma, Cinnamomi Ramulus and ketotifen. In rat dorsal skin, the increase of vascular permeability which was induced by histamine, serotonin or compound 48/80 was inhibited significantly by i.p. injection of Amomi Semen, Asiasari Radix, Cimicifugae Rhizoma, Scutellariae Radix and ketotifen. Armeniacae Semen and Liriopis Tuber which had not inhibited hyaluronidase activity did not inhibit 48-hr PCA and the increase of histamine, serotonin or compound 48/80-induced vascular permeability in mice and rats. These results suggest that each water extract of Amomi Semen and Cimicifugae Rhizoma has anti-allergic action.

• 생약학회지
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• 제29권3호
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• pp.169-172
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• 1998

Hyaluronidase is one of the mucopolysaccharide-splitting enzyme and is related to the permeability of the vascular system and inflammation. An anti-hyaluronidase assay guided fractionation of the methanolic extract of Uncariae Ramulus et Uncus has furnished a pentacyclic triterpene, ursolic acid (compound I). Compound I exhibited hyaluronidase inhibitory activity with $IC_{50}$ value of 0.15 mM, and disodium cromoglycate showed the inhibitory activity with $IC_{50}$ value of 1.78 mM as a positive control.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.63-63
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• 2019

The thinning Green ball apple was extracted using water and ethanol and a phenolic concentration of thinning Green ball apple was $50-200{\mu}g/mL$. The water and ethanol extracts of thinning Green ball apple showed 94.69% and 92.24% 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and 100.30% and 99.16% 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity at phenolic concentration of $200{\mu}g/mL$, respectively. The water and ethanol extracts of thinning Green ball apple showed antioxidant protection factor of 1.76 antioxidant protection factor and 1.76 antioxidant protection factor, respectively. The water and ethanol extracts showed 101.46% and 99.64% anti-oxidative effect on thiobarbituric acid reactive substances at phenolic concentration of $200{\mu}g/mL$. Hence, the water and ethanol extracts of thinning Green ball apple can be considered a potential anti-oxidant. The water and ethanol extracts showed 33.28% and 32.14% hyaluronidase inhibition, respectively, at phenolic concentration of $150{\mu}g/mL$. The water and ethanol extracts showed 47.33% and 40.92% elastase inhibition and 46.19% and 65.58% collagenase inhibition at phenolic concentration of $200{\mu}g/mL$, respectively. About these experiments, thinning Green ball apple was found to exhibit anti-oxidation activity as well as hyaluronidase, elastase and collagenase inhibitory activities. Therefore, thinning Green ball apple can be considered a potential source for functional food.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.663-665
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• 2003

본 연구에서는 항염증 및 항산화 활성을 갖는 유용한 물질을 검색하기 위해 40여 가지 생약재를 에탄올을 이용하여 추출한 후 hyaluronidase 억제 및 라디칼 소거 활성을 측정하였다. hyaluronidase 활성을 저해시키는 물질로서 황기, 목단피, 오미자 등이 높은 효과를 보였고, 목단피, 백작약, 복분자 등은 유리 라디칼종에 대해 높은 소거활성을 나타내어 조직 손상 및 염증 질환에 매우 유효한 소재로서 이용될 수 있으리라 사료된다.

• 생약학회지
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• 제35권3호통권138호
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• pp.184-188
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• 2004

This study was carried out to evaluate glutathione S-transferase (GST) activity and hyaluronidase inhibitory effect of medicinal plants. The EtOH extracts of 20 species plants were tested. As the result, Acorus gramineus and Pueraria lobata exhibited GST activity. On the continuous experiment, the n-BuOH fraction of Acorus gramineus and the $H_2O$ fraction of Pueraria lobata showed the elevation of GST activity. On the experiment of hyaluronidase inhibitory effect, Acorus gramineus exhibited a potent inhibitory activity. These results suggest that the extract of Acorus gramineus can be applicable for the development of a new anti-inflammatory agent.

• Fisheries and Aquatic Sciences
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• 제25권6호
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• pp.327-334
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• 2022

Marine resources have various biological activities and their constituents are more novel than those of land organisms. Several biologically active constituents have been found in marine organisms. Recently, many studies have reported that marine animals (MAs) can be used as functional ingredients in functional foods or nutraceutical due to their health benefits. However, no studies have extensively investigated the cosmeceutical activities of MAs extracts. Here, 70% ethanol extracts of 38 MAs were investigated for their activities of whitening and anti-aging properties for use as materials in novel cosmeceuticals. Anti-aging activities were determined by skin aging-related enzyme activities (anti-collagenase, anti-elastase, anti-hyaluronidase) and whitening activities (anti-tyrosinase, anti-3,4-dihydroxyl-L-phenylalanine [DOPA] oxidation) evaluated by colorimetric method. Among the 38 MAs, we found that Urechis unicinctus and Petrosia corticata extracts showed the strongest inhibitory effects against tyrosinase and DOPA oxidation, respectively. Our results additionally showed that Protankyra bidentata extract might provide a major source of anti-hyaluronidase and anti-elastase; meanwhile, anti-collagenase effects were similar in most MAs. Overall, these results suggest that extracts of marine animals have potential as a tyrosinase, collagenase, elastase, and hyaluronidase inhibitors. Taken together, MA resources could be considered as a novel cosmeceutical agent to be applied in cosmetic industry.