• Title/Summary/Keyword: Anti-estrogenic

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Antifertility activity of hydro alcoholic extract of Moringa concanensis Nimmo: An ethnomedicines used by tribals of Nilgiris region in Tamilnadu

  • Ravichandiran, V;Suresh, B;Sathishkumar, MN;Elango, K;Srinivasan, R
    • Advances in Traditional Medicine
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    • v.7 no.2
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    • pp.114-120
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    • 2007
  • In the present study, the hydro alcoholic extract of Moringa concanensis and their different fractions were evaluated for it's anti implantation, abortifacient, estrogenic and antiestrogenic activity. Hydro alcoholic extract of Moringa concanensis has showed potent antiimplantation and abortifacient activity at 200 mg/kg and 400 mg/kg respectively and marked estrogenic activity when administered individually and anti estrogenic activity was observed when administered along with ethinyl estradiol (1 ${\mu}g/rat/day$) as well as their different fractions of Moringa concanensis showed significant antiimplantation and abortifacient activity at 100 mg/kg. Moreover, all tested fractions showed significant anti estrogenic activity when administered simultaneously with ethinyl estradiol.

Anti-Estrogenic Activity of Lignans from Acanthopanax chiisanensis Root

  • Lee Sanghyun;Yoo Hye Hyun;Piao Xiang Lan;Kim Ju Sun;Kang Sam Sik;Shin Kuk Hyun
    • Archives of Pharmacal Research
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    • v.28 no.2
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    • pp.186-189
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    • 2005
  • Anti-estrogenic activity of (-)-sesamin (1), helioxanthin (2), savinin (3), taiwanin C (4), and 3­(3,4-dimethoxybenzyl)-2-(3,4-methylenedioxybenzyl)butyrolactone (5) isolated from the root of Acanthopanax chiisanensis was tested using Ishikawa cells. Among them, compound 3 exhibited anti-estrogenic activity ($IC_{50}\;=\;4.86\;{\mu}M$).

Estrogenic and Androgenic Potential of Phthalates and Their Alternatives (프탈레이트류와 그 대체물질의 내분비계 교란독성: 에스트로겐성과 안드로겐성을 중심으로)

  • Kwon, Bareum;Ji, Kyunghee
    • Journal of Environmental Health Sciences
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    • v.42 no.3
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    • pp.169-188
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    • 2016
  • Objectives: Although information on the toxicity of phthalate diesters is readily available, little is known about phthalate alternatives. The present article provides a summary of available information on the toxicity of phthalate diesters and their alternatives, with a special focus on estrogenicity and androgenicity. Methods: We collected a battery of in vitro and in vivo assay data from the literature to assess the estrogenicity/anti-estrogenicity and androgenicity/anti-androgenicity of 15 phthalate diesters and 21 phthalate alternatives. Results: A number of in vitro studies show that certain phthalate diesters can bind to estrogen receptors and have a weak estrogenic potential. However, this potential was not seen in in vivo studies. Phthalate diesters produced anti-androgenic effects in animals by reducing testosterone production. Among them, di-(2-ethyl-hexyl) phthalate (DEHP) was the most potent. While almost all phthalate alternatives have a lower toxic potential than does DEHP, evidence of reproductive toxicity and estrogenic potential were found in several substances. Conclusion: Significant data gaps exist for phthalate alternatives regarding reproductive endocrine disruption, requiring further investigation.

Effects of a Mixture of Cynanchi Wilfordii Radix and Humuli Lupuli Flos Extract on Estrogenic Activities and Anti-Osteoclastogenesis (백수오(白首烏)와 비주화(啤酒花) 복합물의 에스트로겐 활성과 파골세포 분화 억제효과)

  • Park, Dongjun;Lee, Hong Gu;Min, Kyoungin;Park, Hyoungkook;Jin, Mu Hyun;Cho, Ho Song
    • The Korea Journal of Herbology
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    • v.37 no.5
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    • pp.1-8
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    • 2022
  • Objectives : This study aimed to investigate the synergistic effect of combining Cynanchi Wilfordii Radix extract with Humuli Lupuli Flos extract on estrogenic and anti-osteoclastogenic activity. Methods : Estrogenic effect of a mixture of Cynanchi Wilfordii Radix extract and Humuli Lupuli Flos extract (CWHL), Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin (an active ingredient of Cynanchi wilfordii Radix extract) and 8-prenylnaringenin (an active ingredient of Humuli Lupuli Flos extract) were examined by proliferation E-screen assay and expression of estrogen inducible gene, pS2 via Real Time-PCR (RT-PCR) in MCF-7 estrogen responsive cells. And their estrogenic activities were investigated how to modulate Estrogen receptor 𝛽 by binding affinity assay. Inhibitory effect of CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin on RANKL-induced osteoclast differentiation were tested by TRAP (Tartrate-resistant acid phosphatase) staining in osteoclastogenic RAW 264.7 cells. Results : CWHL, Humuli Lupuli Flos extract and 8-prenylnaringenin accelerated the proliferation of MCF-7 and the expression of pS2 in MCF-7. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin bind to estrogen receptor 𝛽. CWHL, Cynanchi Wilfordii Radix extract, Humuli Lupuli Flos extract, caudatin and 8-prenylnaringenin inhibited RANKL-induced osteoclastogenesis in osteoclastogenic RAW 264.7. CWHL is more effective for all markers than Cynanchi Wilfordii Radix extract or Humuli Lupuli Flos extract alone. Conclusions : CWHL may a potential therapeutic agent for menopause and osteoporosis as a natural food resource. CWHL as a natural food source has therapeutic potential in cases of menopause and osteoporosis.

The effects of a Mixture of Puerariae Flos Extract and Citri Unshius Pericarpium Extract on Estrogenic Activities and Osteoclastogenesis (갈화(葛花)와 진피(陳皮) 추출물로 이루어진 복합물의 에스트로겐 활성과 파골세포 분화억제효과)

  • Cho, Hosong;Lee, Boyoung;Lee, Won Kyung;Lee, Jun Ho;Park, Dongjun;Choi, Changil;Jin, Mu Hyun;Roh, Seok-Seon;Ju, Young-Sung
    • The Korea Journal of Herbology
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    • v.35 no.3
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    • pp.1-8
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    • 2020
  • Objectives : In this study, we examined the estrogenic activities and anti-osteo clastogenesis effects of PCE17, a mixture of PE (an extract of Puerariae Flos), and CE (an extract of Citri Unshius Pericarpium). Methods : The estrogenic effect of PCE17, PE and CE were examined by ER-β/ERE reporter gene assay and proliferation assay in 293 T and MCF-7 cells. The expression of estrogen-responsive gene and protein were checked by Real Time-PCR (RT-PCR) and Western blotting in MCF-7 cells. Inhibitory effect of PCE17, PE and CE on RANKL-induced osteoclast differentiation were evaluated by TRAP staining and RT-PCR in primary osteoclast precursors from rat bone marrow cells. Results : PCE17 and PE bind to ERs (estrogen receptors) and show estrogenic activities in 293T cells. They also stimulated the proliferation of MCF-7 cells and increased the expression of ER response gene, pS2. Tectorigenin, an active ingredient of PE, shows similar estrogenic activities in MCF-7 cells. PCE17 and CE inhibited RANKL-induced osteoclastogenesis in rat primary osteoclast precursor cells and down-regulated the osteoclast-specific genes of Nfatc1, Ctsk, and Acp5. Conclusions : In conclusion, PCE17 may have therapeutic potential in cases of menopause and osteoporosis.

Estrogenic activity of Pomegranate extract in MCF7-ERE cells

  • Cho minjungo;An Jinyoung;Lansky Ephraim
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 2003.05a
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    • pp.192-192
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    • 2003
  • Pomegranate, a small tree originating in Orient, belongs to Punicaceae family. The seeds contain an oil of which about 80% is rare trans 18 carbon fatty acid (punicic acid), and have highest botanical concentration of a sex steroid, estrone. Pharmacological properties of pomegranate extract have been studied, with anti-microbial, anti-parasitic, ant-viral, and anti-cancer effects. We have examined the estrogenic activity of the pomegranate extracts using MCP-7-ERE cells. MCF-7-ERE cells, stably transfected with pERE-Luc were treated various amount of pomegranate extract and after overnight treatments, luciferase activity were measured. Estradiol(E2) dose dependently induced luciferase activity in this cell and maximal response is obtained at 100pM E2.

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STUDY OF RESVERATROL AND ITS DERIVATIVES ON THE REGULATION OG GENE EXPRESSION IN MCF-7 CELLS TRANSFECTED WITH EITHER pERE-LUC OR phCYP1A1-LUC

  • Joung, Ki-Eun;Kim, Yeo-Woon;Sheen, Yhun-Yhong
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2001.11a
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    • pp.111-111
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    • 2001
  • Resveratrol (trans-3,4',5-trihydroxystilbene), which is a polyphenolic compound found in a variety of plants such as grapes and wine, has been reported to have a variety of anti-inflammatory, anti-platelet, and anti-carcinogenic effects. Recently resveratrol of was reported to serve as an estrogen agonist in MCF-7 cells Based on its structural similarity to diethylstilbestrol, a synthetic estrogen, we examined whether resveratrol and its derivatives might be estrogenic using stable MCF-7-ERE cells. Resveratrol functioned as a superagonist at high concentrations (i.e., produced a greater maximal transcriptional response than estradiol) Among the resveratrol derivatives, 10 compounds showed significant estrogenic activity.

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Anti-proliferative Effects of Daidzein, Baicalein, Hesperidin and Ursolic Acid on Human Breast Cancer Cells Stimulated by Estrogenic Compounds (에스트로겐성 물질에 의해 자극된 인간 유방암 세포 증식에 대한 다이드제인, 바이칼레인, 헤스페리딘 및 우르솔산의 억제 효과)

  • Lee, Mi-Nam;Lee, Su-Yel;Lee, Hyun-Jae;Seok, Jeong-Ho;Lee, Choong-Jae
    • YAKHAK HOEJI
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    • v.54 no.3
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    • pp.168-173
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    • 2010
  • We investigated whether three flavonoids including daidzein, baicalein, hesperidin and ursolic acid, a triterpene acid, affect proliferation of MCF-7 human breast cancer cells stimulated by estrogenic compounds. Ursolic acid and baicalein inhibited proliferation of MCF-7 cells induced by PhIP, a food-derived carcinogen with estrogenic activity. Daidzein and hesperidin inhibited estradiol-induced proliferation of MCF-7 cells. These compounds should be further investigated for the possible involvement in signaling pathway after estrogen receptor binding in breast cancer cells.

Estrogenic activity of Pomegranate extract in MCF7-ERE cells

  • Cho, Min-Jung;An, Jin-Young;Sheen, Yhuny-Hong;Lansky, Ephraim;Kong, Gu
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.177.2-178
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    • 2003
  • Pomegranate, a small tree originating in Orient, belongs to Punicaceae family. The seeds contain an oil of which about 80% is rare trans 18 carbon fatty acid (punicic acid), and have highest botanical concentration of a sex steroid, estrone. Pharmacological properties of pomegranate extract have been studied, with anti-microbial, anti-parasitic, anti-viral. and anti-cancer effects. (omitted)

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