• 대한화장품학회지
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• 제41권1호
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• pp.35-43
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• 2015

파극천은 전통적으로 피부염증 치료에 사용되어온 약초이다. 본 연구에서는 파극천 뿌리를 고온($132^{\circ}C$) 및 고압($1.2kgf/cm^2$)에서 15분 동안 처리한 후 추출물을 분리하여 이들의 MMP-1 효소 억제 효능을 확인하였다. 고온고압 처리된 파극천 추출물의 성분변화를 확인한 결과, 고온고압 처리가 파극천 추출물의 페놀 및 플라보노이드 함량을 약 1.5배 이상 증가시키는 것으로 나타났다. DPPH 및 superoxide 라디칼 소거능을 확인해 본 결과 $500{\mu}g/mL$에서 각각 79.25%, 94.5%의 라디칼 소거 효과가 나타났으며, 기존 추출물 대비 농도 의존적으로 활성 증가를 확인하였다. 또한, 항염 효과를 5-LOX 및 COX-2 저해능을 통해 확인한 결과 고온고압 처리된 파극천 추출물에서 약 45% 증가된 저해능이 나타났다. 이에 자외선에 대한 고온고압 처리된 파극천 추출물의 피부 콜라겐 단백질 분해효소 발현 저해 양상도 농도 의존적으로 발현이 억제되었으며 약 16% 정도 저해 효과가 유의적으로 증가되었다. UVB의 세포보호 효과도 고온고압 처리된 파극천 추출물을 함께 처리하였을 때 세포생존율이 증가됨을 확인할 수 있었다. 결론적으로 파극천 추출물에 고온고압 처리를 통해 기존 추출물보다 뛰어난 항산화, 항염 및 MMP-1 발현 억제 효과의 증가를 확인하였으며, 앞으로 기능성 소재로서 화장품에 응용될 수 있을 것으로 사료된다.

• Journal of Ginseng Research
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• 제39권3호
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• pp.250-256
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• 2015

Background: It has been known that ginseng can be applied as a potential nutraceutical for memory impairment; however, experiments with animals of old age are few. Methods: To determine the memory enhancing effect of red ginseng, C57BL/6 mice (21 mo old) were given experimental diet pellets containing 0.12% red ginseng extract (approximately 200 mg/kg/d) for 3 mo. Young and old mice (4 mo and 21 mo old, respectively) were used as the control group. The effect of red ginseng, which ameliorated memory impairment in aged mice, was quantified using Y-maze test, novel objective test, and Morris water maze. Red ginseng ameliorated age-related declines in learning and memory in older mice. In addition, red ginseng's effect on the induction of inducible nitric oxide synthase and proinflammatory cytokines was investigated in the hippocampus of aged mice. Results: Red ginseng treatment suppressed the production of age-processed inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-${\alpha}$, and interleukin-$1{\beta}$ expressions. Moreover, it was observed that red ginseng had an antioxidative effect on aged mice. The suppressed glutathione level in aged mice was restored with red ginseng treatment. The antioxidative-related enzymes Nrf2 and HO-1 were increased with red ginseng treatment. Conclusion: The results revealed that when red ginseng is administered over long periods, age-related decline of learning and memory is ameliorated through anti-inflammatory activity.

• Journal of Applied Biological Chemistry
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• 제54권2호
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• pp.101-107
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• 2011

Aging in females is associated with a reduced metabolic function, increased incidence of neurodegenerative diseases, and cognitive dysfunction, as a result of loss in gonadal function. The change can alter the states of phosphorylation on the proteins, which cause dramatic changes in the cellular location or activity of the proteins. In this study, the differential phosphorylation of the proteins responsible for the functions related to cognition was studied using the ovariectomized adult rats. Phosphoproteomic analysis using the cerebral and hippocampal tissues could identify 51 differentially phosphorylated proteins including 12 proteins for energy metabolism, 8 cytoskeletal proteins, 6 signaling proteins, and other functional proteins in the ovariectomized rats. Further, anti-oxidative enzymes, superoxide dismutase and peroxiredoxin-2, which are known to be inactivated by phosphorylation, were found to be differentially phosphorylated in the cerebellum and hippocampus of the ovariectomized rats, respectively. Many of the deactivated proteins by differential phosphorylation identified in this study were overlapped to those of Alzheimer's disease cases. These results will provide information for neurodegenerative learning and memory impairments in women as brought about by menopause.

• 생약학회지
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• 제44권1호
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• pp.47-52
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• 2013

We invesitagat the effects of antioxidant and suppression of MMP-1 in UV-irradiated human dermal fibroblasts (HDF) by schizandrin isolated from Schisandra chinensis. Ultrasonic technique was used to extract schizandrin from S. chinensis. The higher contents of schizandrin (6.34 mg/ml) in 70% EtOH extract obtained by ultrasonic treatment at 20 MHz for 2 h during 10 days. Schizandrin had antioxidant activities. Schizandrin (1-10 ${\mu}g/ml$) induced a significant dose-dependent decrease in the expression fo MMP-1 protein. These results suggest that schizandrin is a potential candidate for the prevention and treatment of skin aging.

• 생약학회지
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• 제46권4호
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• pp.295-302
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• 2015

Cynanchi wilfordii Radix roots have been utilized as traditional medicine for variety of diseases including diabetes mellitus, aging progression and scavenging free radicals, enhancing immunity, reducing high serum cholesterol, and anti-tumor activity. However, the mechanisms underlying this effect remain poorly understood. The principal objective of this study was to determine the effect of cynandione A on osteoclast cells. Thus, we was isolated cynandione A from Cynanchi wilfordii Radix roots and evaluated the effect of cynandione A on receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. We found that cynandione A significantly inhibited osteoclast differentiation stimulated-RANKL in RAW 264.7 cells. Cynandione A conspicuously inhibited the mRNA and protein expression of matrix metallopeptidase 9 (MMP-9), tartrate-resistant acid phosphatase (TRAP) in cynandione A treated with RANKL. Taken together, our results demonstrated that Cynanchi Wilfordii Radix may be useful treatment option of bone-related disease such as osteoporosis leads to fracture of bone and rheumatoid arthritis.

• 자연과학논문집
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• 제19권1호
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• pp.37-44
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• 2008

새로운 기능성 과실주 개발의 일환으로 영동 지역에서 재배되고 있는 야생 머루를 이용하 머루 발효주를 제조하여 이들의 품질특성과 생리기능성을 조사하였다. 머루 발효주의 에탄올 함량은 11.0% 이었고 총산함량과 휘발산 함량은 각각 0.63%와 0.0402%이었다. 또한 총 기호도는 신맛이 살아있고 단맛이 적어 우수하였고 노화억제에 관련된 항산화 활성이 41.6% 로 우수하였다. 따라서 본 연구의 머루 발효주는 품질이 우수한 과실주로 사료된다.

• 한국융합학회논문지
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• 제10권7호
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• pp.267-279
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• 2019

본 연구는 지실추출물과 나린진의 햇빛으로부터 피부 보호 및 주름개선에 대한 연구를 하였다. HR-1(무모쥐)에 햇빛을 조사하여 상피 두께의 변화와 진피의 90% 정도를 차지하는 콜라겐 섬유의 변화를 확인하였다, 콜라게나아제의 억제 효능도 주름에 중요하게 작용하므로 같이 확인하였다. 실험은 나린진과 지실추출물을 사용하여 효능을 검증하였다. 첫째: 지실과 나린진의 성분을 분석하였다. 둘째: DPPH로 항산화 능력을 확인하였다. 셋째: 콜라겐의 변화를 측정하였다. 연구 결과로 지실추출물 및 나린진이 피부 상피두께 억제와 피부의 콜라겐 두께가 상승하였고 콜라겐 분해 효소 억제 및 주름이 대조군에 비하여 감소되었으며 유의한 차이가 나타났다.

• Journal of Microbiology and Biotechnology
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• 제33권1호
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• pp.135-141
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• 2023

Protection of skin cells from chronic infrared-A (IRA) irradiation is crucial for anti-photoaging of the skin. In this study, we investigated the protective activity of Rg3(S) and Rg3(S)-incorporated anionic soybean lecithin liposomes (Rg3/Lipo) with a size of approximately 150 nm against IRA-induced photodamage in human fibroblasts. The formulated Rg3/Lipo showed increased solubility in aqueous solution up to a concentration of 200 ㎍/ml, compared to free Rg3(S). In addition, Rg3/Lipo exhibited superior colloidal stability in aqueous solutions and biocompatibility for normal human dermal fibroblasts (NHDFs). After repeated IRA irradiation on NHDFs, elevated levels of cellular and mitochondrial reactive oxygen species (ROS) were greatly reduced by Rg3(S) and Rg3/Lipo. In addition, cells treated with Rg3/Lipo exhibited noticeably reduced apoptotic signals following IRA irradiation compared to untreated cells. Thus, considering aqueous solubility and cellular responses, Rg3/Lipo could serve as a promising infrared protector for healthy aging of skin cells.

• Nutrition Research and Practice
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• 제17권5호
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• pp.870-882
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• 2023

BACKGROUND/OBJECTIVES: Obesity is a major risk factor for metabolic syndrome, a global public health problem. Mentha canadensis (MA), a traditional phytomedicine and dietary herb used for centuries, was the focus of this study to investigate its effects on obesity. MATERIALS/METHODS: Thirty-five male C57BL/6J mice were randomly divided into 2 groups and fed either a normal diet (ND, n = 10) or a high-fat diet (HFD, n = 25) for 4 weeks to induce obesity. After the obesity induction period, the HFD-fed mice were randomly separated into 2 groups: one group continued to be fed HFD (n = 15, HFD group), while the other group was fed HFD with 1.5% (w/w) MA ethanol extract (n = 10, MA group) for 13 weeks. RESULTS: The results showed that body and white adipose tissue (WAT) weights were significantly decreased in the MA-supplemented group compared to the HFD group. Additionally, MA supplementation enhanced energy expenditure, leading to improvements in plasma lipids, cytokines, hepatic steatosis, and fecal lipids. Furthermore, MA supplementation regulated lipid-metabolism-related enzyme activity and gene expression, thereby suppressing lipid accumulation in the WAT and liver. CONCLUSIONS: These findings indicate that MA has the potential to improve diet-induced obesity and its associated complications, including adiposity, dyslipidemia, hepatic steatosis, and inflammation.

• International Journal of Molecular Medicine
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• 제43권2호
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• pp.1033-1040
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• 2019

Protein kinase casein kinase 2 (CK2) is important in the regulation of cell proliferation and death, even under pathological conditions. Previously, we reported that CK2 regulates the expression of heme oxygenase-1 (HO-1) in stress-induced chondrocytes. In the present study, it was shown that CK2 is involved in the dedifferentiation and cellular senescence of chondrocytes. Treatment of primary articular chondrocytes with CK2 inhibitors, 4,5,6,7-terabromo-2-azabenzimidazole (TBB) or 5,6-dichlorobenzimidazole 1-β-D-ribofuranoside (DRB), induced an increase in senescence-associated β-galactosidase (SA-β-gal) staining. In addition, TBB reduced the expression of type II collagen and stimulated the accumulation of β-catenin, phenotypic markers of chondrocyte differentiation and dedifferentiation, respectively. It was also observed that the abrogation of CK2 activity by CK2 small interfering RNA induced phenotypes of chondrocyte senescence. The association between HO-1 and cellular senescence was also examined in CK2 inhibitor-treated chondrocytes. Pretreatment with 3-morpholinosydnonimine hydrochloride, an inducer of the HO-1 expression, or overexpression of the HO-1 gene significantly delayed chondrocyte senescence. These results show that CK2 is associated with chondrocyte differentiation and cellular senescence and that this is due to regulation of the expression of HO-1. Furthermore, the findings suggest that CK2 is crucial as an anti-aging factor during chondrocyte senescence.