• 한국버섯학회지
• /
• 제12권1호
• /
• pp.1-7
• /
• 2014

This study was initiated to investigate antioxidant, anti-acetylcholinesterase, and xanthine oxidase inhibitory activities and properties of fruiting bodies, mycelia, and fermentation culture filtrates from Phellinus igniarius. The contents of total phenols and flavonoid of fruit bodies, mycelia, and culture filtrate were 15.35-1.36 mg/g, 10.35-7.85 mg/g, and 8.25-5.36 mg/g. The 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging abilities of the extracts from the fruiting bodies, mycelia, and culture filtrates were 90.25-95.60%, 78.82-85.24%, and 76.32-82.50% at $50-400{\mu}g/mL$, respectively. The chelating ability of fruiting body extract on ferrous ions was higher than those of mycelia and culture filtrates tested. The anti-acetylcholinesterase inhibitory activity of the fruiting body extract at 400 ${mu}g/mg$ exhibited 91.10% on AChE, which is lower than that of positive control, galanthamine (94.82%). The xanthine oxidase inhibitory activities of the fruiting bodies, mycelia, and culture extract were 85.47%, 78.13%, and 72.49% at 400 ${\mu}g/mL$, respectively. Overall, the fruiting body extract has better anti-acetylcholinesterase, antioxidant and xanthine oxidase inhibitory activities than those from mycelia and culture filtrate.

• 한국균학회지
• /
• 제44권4호
• /
• pp.314-317
• /
• 2016

To develop a new anti-dementia acetylcholinesterase (AChE) inhibitor from edible mushrooms, AChE inhibitory activities were determined on water and ethanol extracts of various edible mushrooms from oriental medicine markets and agriculture markets. As a result, the 70% ethanol extract from Pholiota adiposa fruiting body had the highest AChE inhibitory activity of 30.6, and its water extract also had an AChE inhibitory activity of 23.8%. Therefore, we finally selected P. adiposa as a potent anti-dementia AChE inhibitor-containing mushroom. The AChE inhibitor of P. adiposa was maximally extracted when its fruiting body was treated with water for 3hr at $70^{\circ}C$ and 70% ethanol for 12 hr at $70^{\circ}C$, respectively.

• 한국균학회지
• /
• 제47권4호
• /
• pp.337-346
• /
• 2019

본 연구에서는 버섯으로부터 항치매성 건강소재를 개발하고자 주요 식용 및 약용 버섯들의 물과 에탄올 추출물들을 제조한 후 이들의 수율과 acetylcholinesterase 저해활성을 측정하여 우수 버섯을 선발하였다. 또한 선발된 우수버섯에 함유되어있는 항치매성 acetylcholinesterase 저해 물질의 추출 최적조건을 검토하였고, 이 저해물질을 부분정제한 후 PC12 신경세포사 저해 활성을 측정하여 항치매 효능을 검증하였다. 시료 버섯들의 물 추출물과 70% 에탄올 추출물을 각각 제조하여 이들의 acetylcholinesterase 저해 활성을 측정한 결과 물 추출물의 수율이 40%로 높고 acetylcholinesterase 저해 활성도 20.2% (IC₅₀: 12.4 mg)로 우수한 흑타리버섯을 acetylcholinesterase 저해물질을 함유한 우수 버섯으로 최종 선발하였다. 흑타리버섯 자실체중의 항치매성 acetylcholinesterase 저해물질의 추출 최적 조건은 흑타리버섯 분말을 1:30으로 증류수에 현탁 시킨 후 30℃에서 6시간 추출하는 조건이었다. 흑타리버섯 자실체의 항치매성 acetylcholinesterase 저해물질을 한외여과와 gel 여과 등으로 부분정제한 후 신경세포 PC12의 세포치사 억제에 미치는 영향을 조사한 결과 부분정제 물질을 50 ㎍/mL 처리했을 때 세포치사율이 17.0%로 낮아져서 흑타리버섯 부분정제물이 치매 진행과정을 억제하거나 지연시키는 효과를 보이는 것으로 사료된다.

• 한국균학회지
• /
• 제46권4호
• /
• pp.447-457
• /
• 2018

In this paper, the screening of potent acetylcholinesterase (AChE) inhibitor - producing yeasts from wild yeasts and the condition for the production of anti-dementia AChE inhibitors are described. Among one hundred and seven non-pathogenic wild yeast strains from the waters and soils of three main rivers in Daejeon metropolitan city and midstream of Yeongsangang river in Sangju, sporogenous Saccharomyces cerevisiae WJSL0113 and asporogenous Wickerhamomyces anomalus JSF0128 were selected as useful strains for the production of potent AChE inhibitors. The AChE inhibitors of S. cerevisiae WJSL0113 and W. anomalus JSF0128 had a maximum yield when they were incubated in yeast extract-peptone-dextrose media (pH 6.0 in S. cerevisiae WJSL0113 and pH 5.0 in W. anomalus JSF0128) for 18 hr at $30^{\circ}C$, respectively.

• 생약학회지
• /
• 제48권3호
• /
• pp.243-247
• /
• 2017

To find the anti-Alzheimer's activity of Cacalia hastata var. orientalis (Compositae), caffeoylquinic acids were analyzed in this plant by HPLC and the inhibitory activities of the extract on cholinesterases including acetylcholinesterase (AChE) and butyrylcholinesterse (BChE) and peroxynitrite ($ONOO^-$) were assayed. Sum of caffeoylquinic acids in the 80% MeOH extract was quantitatively higher in leaves (35.8% of the extract) than in stems (3.7%). The compound, 3,5-dicaffeoylquinic acid, was contained in the highest amount in the leaf (44.32 mg/g dry weight). The $IC_{50}s$ of the 80% MeOH extract were shown to be $67.45{\mu}g/ml$ for AChE, and $8.59{\mu}g/ml$ (for $ONOO^-$), respectively, suggesting that the leaves would have the anti-Alzheimer's activity due to the high content of caffeoylquinic acids.

• 한국자원식물학회지
• /
• 제32권4호
• /
• pp.270-274
• /
• 2019

국화과에 속하는 단풍취의 추출물이 가지는 알츠하이머에 대한 효과를 알기 위하여 AChE에 대한 억제효과와 peroxynitrite 소거효과를 연구하였다. 단풍취 추출물은 AChE에 대한 억제효과로서 그 $IC_{50}$가 $73.4{\mu}g/ml$이었으며 peroxynitrite 소거효과에 관한 $IC_{50}$는 $8.60{\mu}g/mL$이었으므로 알츠하이머 질환에 유익할 것으로 판단된다. 단풍취에 가장 많이 함유된 성분인 3,5-dicaffeoylquinic acid 성분의 활성도 이와 유사한 활성을 보였으므로 단풍취 추출물의 효과는 주로 이 화합물의 활성에 따른 것으로 예상된다. Caffeoylquinic acid가 알츠하이머 질환에 효과있다는 보고가 있으므로 이 식물의 잎과 줄기 추출물에서 6종의 지표물질을 이용하여 정량법을 확립한 후 정량하였다. 그 결과 잎에서는 성분 함량이 25.1%를 차지하여 줄기의 함량보다 훨씬 높았다. 특히 잎 추출물 중 3,5-dicaffeoylquinic acid의 함량이 145.6 mg/g에 달하여 알츠하이머 질환 치료에 유익할 것으로 예상된다.

• 생약학회지
• /
• 제46권1호
• /
• pp.6-11
• /
• 2015

To clarify chemical constituents of Clematis heracleifolia, isolation and structure elucidation of the underground parts of C. heracleifolia were performed. Five compounds were isolated from $CHCl_3$ and n-BuOH soluble fraction of this plant. On the basis of spectral and physico-chemical data, the structure of isolated compounds were identified as coniferyl alcohol (1), scoparone (2), (+)-lariciresinol (3), phytosterols (4), and daucosterol (5), respectively. All compounds are isolated from this plant for the first time. To evaluate anti-acetylcholinesterase activity of the isolated compounds, compounds 1, 2, 3, and 5 were tested inhibition activity against acetylcholinesterase. Among tested compounds, daucosterol (5) showed acetylcholinesterase inhibitory activity with $IC_{50}$ value of $6.1{\mu}M$.

• BMB Reports
• /
• 제31권3호
• /
• pp.296-302
• /
• 1998

A potentiometric flow injection biosensor for the analysis of anti-cholinesterases (anti-ChEs), based on inhibition of enzyme activity, was developed. The sensor system consists of a reactor with acetylcholinesterase (AChE) immobilized on controlled pore glass and a detector with an $H^{+}-selective$ PVC-based membrane electrode. The principle of the analysis is based on the fact that the degree of inhibition of AChE by an anti-ChE is dependent on the concentration of the anti-ChE in contact with AChE. The sensor system was optimized by changing systematically the operating parameters of the sensor to evaluate the effect of the changes on sensor response to ACh. The optimized biosensor was applied to the analysis of paraoxon, an organophosphorus pesticide. Treatment of the inhibited enzyme with pyridine-2-aldoxime fully restored the enzyme activity allowing repeated use of the sensor.

• 한국식품저장유통학회지
• /
• 제23권2호
• /
• pp.239-245
• /
• 2016

본 연구는 선행 연구된 삼채뿌리의 뛰어난 영양성을 토대로 기능성 식품소재로의 이용가능성을 확인하고 활용도를 높이고자 국내산 및 미얀마산 삼채뿌리의 항산화 및 생리활성을 측정하였다. 삼채뿌리의 총 폴리페놀과 총 플라보노이드 함량은 국내산 삼채뿌리가 각각 5.27 mgGAE/g, 0.35 mgQE/g, 미얀마산 삼채뿌리가 각각 4.80 mgGAE/g, 0.24 mgQE/g으로 국내산 삼채뿌리가 더 높은 함량을 나타냈다. 삼채뿌리의 항산화력을 측정한 결과 DPPH 라디칼 소거능은 미얀마산이 더 높은 활성을 보였지만, ABTS 라디칼 소거능과 $Fe^{2+}$ 킬레이팅 활성, 환원력은 국내산이 더 높은 활성을 보였다. ${\alpha}-Glucosidase$ 저해활성은 국내산의 저해활성이 미얀마산 삼채뿌리의 저해활성보다 더 높았으며 대조구인 acarbose에 비해서도 우수한 저해활성을 보였다. Acetylcholinesterase 저해 활성을 측정한 결과, 50 mg/mL 농도에서 국내산 삼채뿌리가 51.44%로 44.33%의 저해활성을 보인 미얀마산 삼채뿌리보다 더 높은 저해활성을 보였다. 본 연구를 통해 삼채뿌리 특히 국내산 삼채뿌리는 높은 항산화 활성, ${\alpha}-glucosidase$ 및 acetylcholinesterase 저해 활성을 보여 혈당조절제, 치매예방 및 개선제로써의 활용가능성이 큰 것으로 확인되었으며, 이로써 기능성 식품소재로의 활용이 증가될 것으로 판단된다. 하지만 향후 국내산 삼채의 우수성을 검증하기 위하여 여러 지역에서 재배된 삼채의 기능성 분석이 필요할 것으로 판단된다.

• Food Science and Biotechnology
• /
• 제17권1호
• /
• pp.95-101
• /
• 2008

Some biological activities of Beauveria bassiana were studied to elucidate pharmacological function of B. bassiana-infected larva of the silkworm. The mycelium consisted mainly of carbohydrate (65.8%), followed by protein (15.9%) and fat (8.3%). Glucose (68.8%), mannose (7.1%), and galactose (6.1%) were major components in carbohydrates. Ten amino acids including glutamine, threonine, valine, aspartic acid, alanine, leucine, serine, glycine, arginine, and isoleucine were found in protein as major amino acids. Various extracts were prepared from the freeze-dried mycelium of B. bassiana by systemic extraction and their biological activities were investigated. Among tested fractions, the hot-water extract (HW) contributed significantly to the anti-coagulant activity, anti-complementary activity, and stimulation of intestinal immune system. The methanol extract (ME) increased acetylcholinesterase (AChE) inhibition activity and reactive oxygen species (ROS) scavenging activity.