• Title/Summary/Keyword: Anti aging

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Multi-class Classification of Histopathology Images using Fine-Tuning Techniques of Transfer Learning

  • Ikromjanov, Kobiljon;Bhattacharjee, Subrata;Hwang, Yeong-Byn;Kim, Hee-Cheol;Choi, Heung-Kook
    • Journal of Korea Multimedia Society
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    • v.24 no.7
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    • pp.849-859
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    • 2021
  • Prostate cancer (PCa) is a fatal disease that occurs in men. In general, PCa cells are found in the prostate gland. Early diagnosis is the key to prevent the spreading of cancers to other parts of the body. In this case, deep learning-based systems can detect and distinguish histological patterns in microscopy images. The histological grades used for the analysis were benign, grade 3, grade 4, and grade 5. In this study, we attempt to use transfer learning and fine-tuning methods as well as different model architectures to develop and compare the models. We implemented MobileNet, ResNet50, and DenseNet121 models and used three different strategies of freezing layers techniques of fine-tuning, to get various pre-trained weights to improve accuracy. Finally, transfer learning using MobileNet with the half-layer frozen showed the best results among the nine models, and 90% accuracy was obtained on the test data set.

Malaria Epidemic Prediction Model by Using Twitter Data and Precipitation Volume in Nigeria

  • Nduwayezu, Maurice;Satyabrata, Aicha;Han, Suk Young;Kim, Jung Eon;Kim, Hoon;Park, Junseok;Hwang, Won-Joo
    • Journal of Korea Multimedia Society
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    • v.22 no.5
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    • pp.588-600
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    • 2019
  • Each year Malaria affects over 200 million people worldwide. Particularly, African continent is highly hit by this disease. According to many researches, this continent is ideal for Anopheles mosquitoes which transmit Malaria parasites to thrive. Rainfall volume is one of the major factor favoring the development of these Anopheles in the tropical Sub-Sahara Africa (SSA). However, the surveillance, monitoring and reporting of this epidemic is still poor and bureaucratic only. In our paper, we proposed a method to fast monitor and report Malaria instances by using Social Network Systems (SNS) and precipitation volume in Nigeria. We used Twitter search Application Programming Interface (API) to live-stream Twitter messages mentioning Malaria, preprocessed those Tweets and classified them into Malaria cases in Nigeria by using Support Vector Machine (SVM) classification algorithm and compared those Malaria cases with average precipitation volume. The comparison yielded a correlation of 0.75 between Malaria cases recorded by using Twitter and average precipitations in Nigeria. To ensure the certainty of our classification algorithm, we used an oversampling technique and eliminated the imbalance in our training Tweets.

Analysis of Texture Features and Classifications for the Accurate Diagnosis of Prostate Cancer (전립선암의 정확한 진단을 위한 질감 특성 분석 및 등급 분류)

  • Kim, Cho-Hee;So, Jae-Hong;Park, Hyeon-Gyun;Madusanka, Nuwan;Deekshitha, Prakash;Bhattacharjee, Subrata;Choi, Heung-Kook
    • Journal of Korea Multimedia Society
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    • v.22 no.8
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    • pp.832-843
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    • 2019
  • Prostate cancer is a high-risk with a high incidence and is a disease that occurs only in men. Accurate diagnosis of cancer is necessary as the incidence of cancer patients is increasing. Prostate cancer is also a disease that is difficult to predict progress, so it is necessary to predict in advance through prognosis. Therefore, in this paper, grade classification is attempted based on texture feature extraction. There are two main methods of classification: Uses One-way Analysis of Variance (ANOVA) to determine whether texture features are significant values, compares them with all texture features and then uses only one classification i.e. Benign versus. The second method consisted of more detailed classifications without using ANOVA for better analysis between different grades. Results of both these methods are compared and analyzed through the machine learning models such as Support Vector Machine and K-Nearest Neighbor. The accuracy of Benign versus Grade 4&5 using the second method with the best results was 90.0 percentage.

DEVELOPMENT OF POLYETHOXYLATED RETINAMIDE AS AN ANTl-AGINC AGENT

  • Song, Young-Sook;Chung, Bong-Yul;Chang, Min-Youl;Park, Mun-Eok;Lee, Sung-Jun;Cho, Wan-Goo;Kang, Seh-Hoon
    • Proceedings of the SCSK Conference
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    • 1999.10a
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    • pp.145-154
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    • 1999
  • A novel retinol derivative, polyethoxylated retinamide (Medimin A) was synthesized, as an anti-aging agent. Collagen synthesis, skin permeation, stability, and toxicity of Medimin A were evaluated and compared with those of retinol and retinyl palmitate. In vitro collagen synthesis was evaluated by quantitative assay of [$^3H$]-proline incorporation into collagenase sensitive protein in fibroblast cultures. For in vitro skin permeation experiments, Franz diffusion cells (effective diffusion area: $1, 766{\;}\textrm{cm}^2$) and the excised skin of female hairless mouse aged 8 weeks were used The stabilities of retlnoids were evaluated at two different temperature ($25{\;}^{\circ}C$ and $40{\;}^{\circ}C$) and under UV in solubilized state and in OW emulsion. To estimate the safety, acute oral toxicity, acute dermal toxicity, primary skin irritation, acute eye irritation and human patch test were performed The effect of Medimin A on collagen synthesis was similar to that of retinol. The skin permeability of Medimin A was higher than those of retinol and retinyl palmitate. The Medimin A was more stable than retinol and retinyl palmitate. Medimin A was nontoxic in various toxicological tests. These results suggest that Medimin A would be a good anti-aging agent for enhancing bioavailability and stability.

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Fermented sea tangle (Laminaria japonica Aresch) Accelerates Osteoblast Differentiation in murine osteoblastic MC3T3-E1 Cells (MC3T3-E1 골아세포에서 발효 다시마 추출물에 의한 조골세포 분화의 촉진)

  • Nara Jeong;Yung Hyun Choi
    • Journal of Marine Bioscience and Biotechnology
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    • v.15 no.1
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    • pp.24-32
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    • 2023
  • The Laminaria japonica Aresch (Sea tangle) belongs to the brown algae and has a long history as a food material in Asia, including Korea. Recent studies have found that the fermented Sea tangle extract (FST) inhibited the differentiation of osteoclasts and protected osteoblasts from oxidative damage. This study aims to explore the possibility that FST can induce the differentiation of osteoblasts and identify the responsible mechanism. According to our results, FST induced differentiation into osteogenic cells in the presence of osteoblastic MC3T3-E1 cells under non-toxic conditions.. This finding was confirmed by phalloidin staining, increased alkaline phosphatase activity, and calcium deposition. Additionally, it was found that this process was achieved by increasing the expression of key factors involved in osteoblast differentiation, such as runt-related transcription factor-2, osterix, β-catenin, and bone morphogenetic protein-2. Moreover, FST increased autophagy, which may contribute to the maintenance of the bone formation homeostasis, and is associated with the activation of the phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase signaling pathways. Although further research about the bioactive substances contained in FST and the tests of their efficacy are required, the results of this study indicate that FST has incredible applicability as a functional material for maintaining the bone homeostasis.

Effects of Esculetin on TNF-α Induced MMP-1 Expression in Human Fibroblasts Hs68 (인간섬유아세포 Hs68에서 esculetin이 TNF-α로 유도된 MMP-1 발현에 미치는 효과)

  • Bo Hee Jeon;Yong Min Kim
    • Korean Journal of Pharmacognosy
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    • v.54 no.1
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    • pp.1-8
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    • 2023
  • The skin is an important barrier that protects the body from harmful environments such as UV rays. When the skin is repeatedly stimulated, such as UV rays, ROS and pro-inflammatory cytokines are overproduced. As a result, the proteins and nucleic acids that make up the skin are damaged, and aging occurs. Esculetin is known to have anti-inflammatory, antioxidant and UV-induced MMP-1 inhibitory effects. However, the inhibitory effect of MMP-1 on TNF-α-induced fibroblasts is not known. Therefore, in this study, the MMP-1 inhibitory effect of esculetin was confirmed in TNF-α-induced fibroblasts. As a result of confirming the cytotoxicity of esculetin in Hs68 cells by MTT assay, there was no significant toxicity up to 200 µM. As a result of real-time PCR and ELISA, it was confirmed that esculetin inhibited the expression of MMP-1. Esculetin did not inhibit MAPK (ERK, JNK, p38) phosphorylation, but inhibited phosphorylation of the mTOR-p70S6k signaling pathway. In addition, it was confirmed that the phosphorylation of the transcription factor NF-κB was inhibited. These results suggest that esculetin has potential as an anti-aging material.

Target Proteins Involved in Aging Mechanism as an Aging Molecular Marker (노화 분자마커로서 노화기전에 관여하는 타켓 단백질)

  • Kim, Moon-Moo
    • Journal of Life Science
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    • v.26 no.8
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    • pp.983-989
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    • 2016
  • All cells composing of our body undergo their destiny such as proliferation, differentiation, necrosis, apoptosis and senescence depending on their circumstance with time. The errors occurring in these processes develop several aberrations in phenotypes including cancer, inflammation, aging and diseases. New strategy and approach are required to screen anti-aging compounds derived from natural products. Therefore, here we explain the target proteins to play a key role in aging mechanism. In the first place, matrix metalloproteinases (MMPs) are involved in metastasis, chronic inflammation and skin aging as an aging marker. In particular, histone deacetylases (HDACs) give a great attention to aging researchers who try to extend the life span of animal model. In addition, we describe the signaling pathway related to senescence which p53, IGF-1 and SIRT1 play an important role in. Furthermore, autophagy is involved in the signaling pathway associated with aging. Several new compounds modulating the signaling pathway of senescence are introduced in this review. Here, we try to provide a new insight in the molecular basis for the aging mechanism and development of aging marker. In addition, the compounds introduced here could be available for pharmaceutical applications for the prevention and the treatment of diseases related to aging.

Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection

  • Yoo, Jinhee;Park, Kimoon;Yoo, Youngji;Kim, Jongkeun;Yang, Heejin;Shin, Youngjae
    • Food Science of Animal Resources
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    • v.34 no.1
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    • pp.26-32
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    • 2014
  • This study was conducted to examine the effects of eggshell membrane hydrolysates (ESMH) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. Whole ESMH (before fractionation), and fraction I (>10 kDa), fraction II (3-10 kDa), and fraction III (<3 kDa) of the hydrolysates were assessed in this experiment. As lipopolysaccharide (LPS) and IFN-${\gamma}$ caused the inflammation on Raw264.7 cell, whole ESMH and fraction I showed to be effective in inhibiting the induction of cell inflammation depending on the concentration, and also showed outstanding effect to suppress the skin inflammation. Fraction I inhibited collagenase and elastase activities to a greater extent than the other fractions, while all fractions had antibiotic effects at concentrations of 10 mg/disc and 20 mg/disc. In addition, it showed the moisture protection effects of skin on the holding amount and losing amount of moisture in upper-inner arm of the human body with a relatively low loss rate in skin, which confirmed that the hydrolyzed fractions of ESM helps to form the superior protective layer of moisture. It was concluded that ESMH fractions with different molecular weights, especially the 10 kDa fraction, have anti-lipopolysaccharide, anti-IFN-${\gamma}$-induced inflammation, anti-collagenase and elastase activities, and thus can be used as a cosmetic agent to protect skin.

The Regulation of Early Senescence-related SM22 Protein in Human Dermal Fibroblasts by Hovenia dulcis Thunberg Fractions (헛개나무 분획물에 의한 HDF세포의 초기 노화관련 sm22 단백질 조절)

  • Sim, Bo Ram
    • Journal of Life Science
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    • v.30 no.8
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    • pp.680-687
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    • 2020
  • Several studies have proved Hovenia dulcis extracts to be an effective antidote for alcoholism, and they are frequently used in the clinical treatment of alcoholic liver disease. The purpose of this study was to investigate the anti-oxidant and anti-senescent activities of 80% methanol fractions of H. dulcis Thunberg in human dermal fibroblast (HDF) cells. The ethanol ethyl acetate fraction showed the highest inhibition of 2,2-diphenyl-1-picryhydrazyl radical scavenging (RC50=3.6±0.8 ㎍/ml) as well as the highest total phenol content (417.2antiholic liver disease. Extracts from e & Tech., Sungkyunkwan University, progressed in the HDF cells, SM22 expression (p<0.001) and β-galactosidase staining intensity increased (p<0.001). An increase in SM22 expression is associated with slower aging which suggests that its expression is high in early aging. HDF treatment using the ethyl acetate fraction significantly increased production of EGF (p<0.01) and PDGF-BB (p<0.01) while SHH expression was reduced (p<0.05). These results suggest that an 80% ethyl acetate fraction of H. dulcis can suppress early aging and demonstrate the potential application of the fraction as a natural anti-senescence agent.

Effects of Parsley Extract on Skin Anti-aging and Anti-irritation (파슬리추출물의 피부 노화 방지와 자극 완화에 대한 효과)

  • 김수남;이소희;최규호;장이섭;이병곤
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.1
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    • pp.79-83
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    • 2004
  • In order to investigate the beneficial effects of parsely (Petroselinurn sativum) extract on skin, we measured the synthesis of total collagen and type I procollagen in cultured normal human fibroblast (NHF), the synthesis of prostaglandin E$_2$(PCE$_2$), interleukin 1 ${\alpha}$(IL -1 ${\alpha}$) and tumor necrosis factor ${\alpha}$ (TNF ${\alpha}$) in HaCaT cell and we also measured dermal thickness and density in hairless mouse (Female albino hairless mice, Skh:hr-1). As the results, the synthesis of total collagen and type I procollagen were increased 23% and 18% respectively, after 1 $\mu\textrm{g}$/mL parsley extract treatment. The producions of PGE$_2$ induced by UVB irradiation were decreased 60% after 1 $\mu\textrm{g}$/mL parsley extract treatment. The treatment with 1 $\mu\textrm{g}$/mL parsley extract also decreased the synthesis of IL -1 ${\alpha}$ and TNF ${\alpha}$ induced by 10 uM RA, 100 $\mu\textrm{g}$/mL SLS and 30 mJ/$\textrm{cm}^2$ UVB irradiation, After 4 days treatment with 1% parsley extract, the dermal thickness of hairless mouse was increased 1.5 times and the density of dermis was tighter than control. These results indicate that parsley extract have anti-aging and anti-irritation effects on skin.