• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.90-90
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• 2019

Hibiscus syriacus exhibited promising potential as a new source of food and colorants containing various anthocyanins. However, the function of anthocyanins from H. syriacus has not been investigated. In the current study, we evaluated whether anthocyanins from the H. syriacus varieties Pulsae and Paektanshim (PS and PTS) inhibit melanin biogenesis. B16F10 cells and zebrafish larvae were exposed to PS and PTS in the presence or absence of ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH), and melanin contents accompanied by its regulating genes and proteins were analyzed. PS and PTS moderately downregulated mushroom tyrosinase activity in vitro, but significantly decreased extracellular and intracellular melanin production in B16F10 cells, and inhibited ${\alpha}$-MSH-induced expression of microphthalmia-associated transcription factor (MITF) and tyrosinase. PS and PTS also attenuated pigmentation in ${\alpha}$-MSH-stimulated zebrafish larvae. Furthermore, PS and PTS activated the phosphorylation of extracellular signal-regulated kinase (ERK), whereas PD98059, a specific ERK inhibitor, completely reversed PS- and PTS-mediated anti-melanogenic activity in B16F10 cells and zebrafish larvae, which indicates that PS- and PTS-mediated anti-melanogenic activity is due to ERK activation. Moreover, chromatography data showed that PS and PTS possessed 17 identical anthocyanins as a negative regulator of ERK. These findings suggested that anthocyanins from PS and PTS inhibited melanogenesis in vitro and in vivo by activating the ERK signaling pathway.

• Corrosion Science and Technology
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• 제21권5호
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• pp.381-389
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• 2022

Cyclic Voltammetry and weight loss measurements were used to investigate corrosion prevention of tin in a 0.5M citric acid solution containing Anthocyanins extracted from grapes at various concentrations and temperatures. Results showed that the investigated chemicals, Anthocyanins extracted from grapes, performed well as tin corrosion inhibitors in 0.5M citric acid. Increasing the concentration of Anthocyanins increased their corrosion inhibition efficiencies. When the temperature dropped, their inhibition efficiencies, increased indicating that higher temperature tin dissolution predominated the adsorption of Anthocyanins at the surface of tin metal. When inhibitor concentrations were increased, their inhibition efficiencies were also increased. These results revealed that corrosion of tin metal was inhibited by a mixed type of adsorption on the metal surface. The adsorption isotherm of Langmuir governed the adsorption of Anthocyanins. Thermodynamic parameters such as the enthalpy of adsorption, the entropy of adsorption, and Gibbs free energy and kinetic parameters such as activation energy, enthalpy of activation, and entropy of activation were computed and discussed in this study.

• 원예과학기술지
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• 제16권3호
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• pp.381-384
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• 1998

Hibiscus속 (아욱과)의 27개 종에 대한 안토시아닌분포를 조사한 결과 절(section)간 안토시아닌조성의 차이를 관찰하였다. 가장 대표적으로 발견되는 안토시아닌은 delphinidin과 cyanidin의 3-xylosyglucoside와 3-glucoside로, Trichospermum, Fucaria, Trionum, Abelmoschus, Ketmia절에 속하는 종들에서 나타났다. Cyanidin 3-sophoroside는 Lilibiscus절의 종들에서 발견되는 대표적인 안토시아닌이었다. Bombycella절에서는 6가지의 common anthocyanidin의 3-glucoside와 3-malonylglucoside가 검출되었다. 이러한 안토시아닌의 절간 분포 차이는 기존의 형태적 자료에 기초를 둔 section의 구분 범주와 잘 일치하였다. 또한 자가불화합성을 나타내는 section에서 다양한 안토시아닌의 변이를 나타내었다. 이러한 안토시아닌의 변이결과들을 수분생태학과 관련지어 논의하였다.

• 동아시아식생활학회지
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• 제27권4호
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• pp.378-386
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• 2017

This study aimed to estimate daily intake of anthocyanins and to identify major sources of anthocyanins in current Korean dietary patterns in order to implement dietary recommendations for the improvement of Korean health. Sixteen foods were selected based on the availability of food intake and reliable anthocyanin content. Food intake data from the Korea National Health and Nutrition Examination Survey 2014 and anthocyanin content data from earlier investigations were used to calculate the consumption of anthocyanins. Anthocyanin contents of 16 foods varied significantly and exhibited a range of 0~4,009 mg/100 g of fresh weight. Daily intake of anthocyanins was estimated to be 3.3 mg to 95.5 mg in Koreans. Of the 16 foods studied, the source contributing most to anthocyanin intake in the Korean population was plums (35.1%), followed by black beans (17.1%) and grapes (15.2%). These results indicate that major foods contributing to anthocyanin consumption in the Korean dietary pattern are fruits and grains.

• Preventive Nutrition and Food Science
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• 제2권3호
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• pp.191-196
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• 1997

The antioxidative activity of seven different acylated anthocyanin pigments isolated from grape, sweet potato, eggplant and red cabbage was evaluated bvy using linoleic acid autoxidation and rat liver microsomal systems. The acylated anthocyanins were isolated and purified by Amberlite SAD-7, ODS and Sephadex LH-20 column omatography, and preparative HPLC. Most of acylated anthocyanisns exhibited antioxidative activity as strong as $\alpha$-tocoperol, and especially peonidin3-O--(6-O-trans-caffeyl)-2-O-(6-O-trans-feuloylglucopyranosyl)-$\beta$-D-glucopyranosyl-5-O-$\beta$-D-glucopyranoside from purple sweet potato showed the strongest activity, comparable to BHA (not significant, p<0.05) in the linoleic acid system. Meanwhile, two acylated anthocyanins from the pericarps of grape and eggplant inhibited considerably the MDA formation from rat liver microsomal lipid peroxidation induced by FeSO$_4$/$H_2O$$_2$. In particular, malvidin 3-O-(6-O-p-coumaroyl)-$\beta$-D-glucopyranosde from grape pericarps showed the strongest antioxidant activity, comparable to $\alpha$-tocopherol (not significant, p<0.05). These results suggest that the acylated anthocyanins from fruits and vegetables can be used as potential dietary ntioxidants and natural colorants.

• Preventive Nutrition and Food Science
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• 제2권2호
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• pp.83-88
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• 1997

Anthocyanin pigments of purple sweet potato roots (Ipomoea batatas L.) were extracted with 0.5% TFA (Trifluoroacetic acid) in 95% EtOh, and further isolated an purified by Amberlite XAD-7 and ODS column chromatography, and final preparative HPLC. Among nine anthocyanins isolated, the structure of three major anthocyanins were identified as 3-O-(6-O-trans-caffeyl)-2-O-(6-O-trans-caffeylglucopyranosyl)-$\beta$-D-glucopyranosyl-5-O-($\beta$-D-glucopyranosyl)-peonidin, 3-O-(6-O-trans-caffeyl)-2-O-(6-O-trans-feruloyl-glucopyranosyl)-$\beta$-D-glucopyranosyl-5-O-($\beta$-D-glucopyranosyl)-peonidin, and 3-O-(6-O-trans-caffeyl)-2-O-(6-O-p-hydroxylbenzoylglucopyranosyl)-$\beta$-D-glucopyranosyl)-peonidin, by using UV-visible absorption spectra, $^1$H-NMR and FAB-MS analysis.

• 미생물학회지
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• 제47권2호
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• pp.143-150
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• 2011

기능성이 밝혀져 있는 자색고구마를 첨가하여 기호성과 영양뿐만 아니라 인체에 유익한 기능성 동치미 냉면육수 개발을 위한 연구를 수행하였다. 자색고구마를 첨가한 동치미 냉면육수 발효 과정에서 젖산균, pH, 산도, 유기산, 수용성 phenolics, anthocyanin, 및 항산화 활성 변화를 조사하였다. 특히, 자색고구마를 첨가한 경우 발효종기(72시간)에 수용성 phenolics 및 anthocyanins 함량은 각각 1.26 g/L 및 8.59 g/L로 대조실험과 비교하여 각각 5배 및 34배 높았다. DPPH 라디칼 소거 활성 96.80%, $ABTs^{+{\cdot}}$ 라디칼 소거활성 100.82%, 환원력 7.77 ($OD_{700}$), FRAP 6.89 ($OD_{593}$)로 대조실험과 비교하여 각각 4배, 1.2배, 2배 및 3.6배 높은 항산화 활성을 나타내었다. 이상의 결과로부터 동치미 냉면육수의 항산화 활성은 자색고구마의 수용성 phenolics와 anthocyanins과 밀접한 관련이 있는 것으로 판단되었으며, 항산화 활성이 증가된 기능성 동치미 냉면육수 제조가 가능하였다.

• 한국식품저장유통학회지
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• 제7권3호
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• pp.285-290
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• 2000

Anthocyanin 색소원(자색고구마, 꽃양배추, 적양배추, 포도, 흑미, 가지, 무화과)의 색가를 조사하고 이들 중 색가가 높은 자색고구마, 꽃양배추, 적양배추 및 포도과피를 선정하여 이들 색소의 안정성에 미치는 금속이온, ascorbic acid, 자외선 및 가열의 영향을 조사하였다. 자색고구마와 포도과피의 색소는 $Mn^{2+}$에 의해 색소의 안정성이 가장 크게 저하되었으며, 적양배추와 꽃양배추 색소는 모두 $Cu^{2+}$이온에 의해 안정성이 가장 크게 저하되었다. Ascorbic acid와 자외선 및 가열은 anthocyanin 색소의 안정성을 저하시켰는데, 그 정도는 색소원에 따라 달랐다. 이들 요인들에 대한 안정성은 자색고구마 색소가 가장 높았으며, 다음으로 꽃양배추, 적양배추 및 포도과피의 색소 순이었다. 색소원에 따라 색소의 안정성이 달라지는 것은 이들 anthocyanin색소를 구성하는 색소성분의 차이에 기인한다.

• 한국식품과학회지
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• 제52권5호
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• pp.503-509
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• 2020

The present study investigated the effects of dry and moist heat treatments on total phenols, total anthocyanins, and antioxidant activity levels in black chokeberry (Aronia melanocarpa) fruits. Lyophilized chokeberry powder samples were heated in a drying oven at 60, 100, 160, 180, and 200℃ for 20, 40, or 60 min. Finely ground fresh chokeberry fruits were heated in water at 60, 80, and 100℃ for 20 min, and bioactive compound and antioxidant activity levels were measured. The bioactive compounds and antioxidant activity decreased with increasing temperature and treatment duration. Antioxidant activity was preserved at 160℃ or lower without significant loss for dry heating, whereas moist heat treatment increased both bioactive compounds and antioxidant activity with increasing temperature.

• Applied Biological Chemistry
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• 제41권4호
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• pp.257-260
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• 1998

Anthocyanins pigments were isolated from the pigmented rice seeds (cultivar Killimhuk-mi) by a combination of Sephadex LH-20 and preparative high performance liquid chromatographies. Four anthocyanins were identified by their chromatographic, spectral and chemical properties. The major pigment was cyanidin 3-glucoside and cyanidin 3-oxalyl-glucoside was found in the rice for the first time.