• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.10
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• pp.1594-1598
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• 2015

This study was performed to investigate the effects of various acidic treatment for anthocyanin and proanthocyanidin extraction from black bean. Extracts were prepared with 80% methanol solutions containing HCl, acetic, formic, phosphoric, and citric acids of different concentrations (0.1, 0.2, 0.3, 0.5, and 0.7%). Total anthocyanin content ranged from 0.74 mg/g in 0.7% citric acid to 1.74 mg/g in 0.3% HCl, depending on acid type and concentration. The major anthocyanins were delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G), and petunidin-3-glucoside (Pt3G). The highest C3G content was 1.12 mg/g in 0.3% HCl. Proanthocyanidin content ranged from 2.01 mg/g in 0.5% acetic acid to 5.29 mg/g in 0.3% HCl. Thus, acidic condition is a significant factor affecting anthocyanin and proanthocyanidin extraction from black bean, and the optimum extraction conditions were determined as 80% methanol containing 0.3% HCl.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.304-308
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• 1996

The major color component of a Korean pigmented rice (Oeyza sativa var. Suwon 415) was purified with Amberlite XAD-7 column and preparative paper chromatography. The purified pigment was determined as anthocyanin by paper chromatography, UV/Vis and NMR spectroscopy. The $\lambda_{max}$ of the Purified anthocyanin on UV/Vis spectrum were 529 nm and 281 nm. The $A_{440}/A_{529}$ value of the purified anthocyanin was 23% suggesting the presence of 3-glycosidic structure. The aglycone from acid hydrolysis showed bathochromic shift (18 nm) in the presence of $AlCl_3$ indicating that the anthocyanidin contained free adjacent hydroxyl groups such as cyanidin, delphinidin, petunidin or luteolinidin. The sugar moiety obtained from acid hydrolysis was determined as glucose by paper chromatography. The NMR spectra showed that the aglycone was cyanidin and the sugar was ${\beta}-D-glucopyranose$. Thus, the chemical structure of the purified anthocyanin was identified as cyanidin $3-O-{\beta}-D-glucopyranoside$.

• Journal of Bio-Environment Control
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• v.27 no.3
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• pp.213-221
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• 2018

We analyzed the skin coloration and anthocyanin composition of 'Kyoho' and 'Heukboseok' grape berries to determine the cause of poor coloring in 'Kyoho' berry skins under high temperature (HT) at veraison. Although the skin coloration inhibited in both 'Kyoho' and 'Heukboseok' berries under HT for 30 days from veraison, the total anthocyanin content in 'Heukboseok' berry skins increased to the level of control after the end of temperature treatment, but 'Kyoho' did not increase. Malvidin derivatives were most significantly reduced in 'Kyoho' berry skins, followed by those of delphinidin and petunidin. Among individual anthocyanins, diglucosides and acylated malvidin derivatives were most decreased in 'Kyoho' berry skins. Acylated and tri-hydroxylated anthocyanins were reduced more than those of non-acylated and dihydroxylated, respectively. All different types of anthocyanin components in 'Kyoho' berry skins decreased by HT, and they were similar to that of total anthocyanin. In 'Heukboseok' berry skins, accumulations of all different types of anthocyanins were inhibited by HT, and increased to the level of control after the end of the treatment. These results suggest that the poor coloration of 'Kyoho' under HT at veraison was not caused by the decrease of specific anthocyanins but because the whole anthocyanin biosynthesis was suppressed by HT.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.295-303
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• 2021

We analyzed berry skin coloration, anthocyanin accumulation, and plant hormone contents in berry skins to determine the effect of night temperature at veraison on berry skin coloration in 'Kyoho' grapevines (Vitis labruscana L.). Vines were grown under 21, 24, and 27℃ at night for 20 days at veraison, from 40 to 60 days after full bloom (DAFB). Berry skin coloration of 'Kyoho' grapes was more suppressed in 27℃ treated vines, followed by that in 24℃ treated vines, than that in 21℃ treated vines. Cluster and berry weight and soluble solids content was lower in 24 and 27℃ treated vines than in 21℃ treated vines. Anthocyanin started to accumulate from 60 DAFB in berry skin of 21℃ treated vines, and malvidin and total anthocyanin content increased until 100 DAFB. The total and most of the individual anthocyanins decreased in 24 and 27℃ treated vines; however, peonidin did not decrease in 24℃ treated vines compared to that in 21℃ treated vines. Abscisic acid (ABA) peaked at veraison in berry skins of 21℃ treated vines and decreased thereafter until 100 DAFB. The increase in ABA content was inhibited in berry skins of 24 and 27℃ treated vines. Gibberellin (GA) content in berry skins decreased rapidly at veraison, with the decrease being slower under 27℃ than under 21℃. ABA/GA in berry skins of 21℃ treated vines peaked at 60 DAFB and decreased thereafter until 100 DAFB. However, ABA/GA decreased in berry skins of 24 and 27℃ treated vines, with reduced anthocyanin accumulation. Therefore, high night temperature (above 24℃) at veraison suppressed the berry skin coloration of 'Kyoho' grapes with changes in anthocyanin contents and composition due to the decrease in ABA/GA ratio and fruit soluble solids contents.

• Journal of Bio-Environment Control
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• v.28 no.3
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• pp.234-242
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• 2019

We analyzed the anthocyanin accumulation, abscisic acid (ABA), gibberellic acid (GA) contents and metabolic genes expression in berry skins under high temperature (High T) at veraison, in order to investigate the cause of bad coloration of 'Kyoho' grape due to High T in summer season. The coloration of 'Kyoho' grapes was stopped by High T for 10 days from veraison, and the fruit quality was not affected except skin color. Total anthocyanin of skins was decreased by High T treatment and malvidin and peonidin were decreased compared to control. In berry skins, ABA content did not decrease by High T treatment, but it was rather higher than that of control. GA content was increased about two times compared to the control after 10 days of High T treatment, which caused decreased ratio of ABA/GA. Analysis of expression of anthocyanin biosynthetic genes showed that the early biosynthetic genes were not affected by High T and the expression of UFGT was decreased by temperature treatment. ABA biosynthetic gene expressions were not affected by High T and the expression of GA20ox1 and GA2ox1/2, which are known to regulate the biosynthesis and inactivation of GA, were increased and decreased by High T, respectively. Therefore, the bad coloration of 'Kyoho' grapes under the High T at veraison was due to inhibition of anthocyanin biosynthesis of skin, and it was suggested that the anthocyanin biosynthesis was controlled by the ratio of ABA and GA rather than ABA content.