• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.274-282
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• 2012

The object of this study was to assess the efficacy of Polycan from Aureobasidium pullulans SM-2001, which is composed mostly of beta-1,3-1,6-glucan, on osteoarthritis (OA)-induced by anterior cruciate ligament transection and partial medial meniscectomy (ACLT&PMM). Three different dosages of Polycan (85, 42.5, and 21.25 mg/kg) were orally administered once a day for 84 days to male rats a week after ACLT&PMM surgery. Changes in the circumference and maximum extension angle of each knee, and in cartilage histopathology were assessed using Mankin scores 12 weeks after Polycan administration. In addition, cartilage proliferation was evaluated using bromodeoxyuridine (BrdU). As the result of ACLT&PMM, classic OA was induced with increases in maximum extension angles, edematous knees changes, and capsule thickness, as well as decreases in chondrocyte proliferation, cartilages degenerative changes, and loss of articular cartilage. However, these changes (except for capsule thickness) were markedly inhibited in all Polycan- and diclofenac sodium-treated groups compared with OA control. Although diclofenac sodium did not influence BrdU uptake, BrdU-immunoreactive cells were increased with all dosages of Polycan, which means that Polycan treatment induced proliferation of chondrocytes in the surface articular cartilage of the tibia and femur. The results obtained in this study suggest that 84 days of continuous oral treatment of three different dosages of Polycan led to lesser degrees of articular stiffness and histological cartilage damage compared with OA controls 91 days after OA inducement, suggesting that the optimal Polycan dosage to treat OA is 42.5 mg/kg based on the present study.

• 대한기계학회논문집B
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• 제36권11호
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• pp.1081-1090
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• 2012

외상성 관절염 발생시 슬관절에서의 해면골 미세구조 변화 특성에 대한 연구는 부족한 실정이다. 따라서 본 연구에서는 생체 내 미세 단층 촬영을 통해, 소동물(SD rat; 10 마리)의 경골 골단 해면골 미세구조 변화패턴을 연속적으로 추적 관찰하여 정량적으로 분석하였다. 외상성 관절염 군(5 마리)에는 전방십자인대절제술을 실시하였고, 정상군(5 마리)에는 아무런 외과적 처치를 가하지 않았다. 외상성 관절염군은 정상군과 비교시 수술후 8 주까지 약 4-16%의 유의한 골 미세구조 차이를 나타내었다(P<0.05). 동일 기간 동안 골 밀집 정도 및 골 형성 정도는 5-15% 정도로 유의한 차이를 나타내었다(P<0.05). 본 연구는 외상성 관절염 발생에 의한 경골 골단 해면골의 골 미세구조 변화 특성 기준 정립 및 외상성 관절염의 이해와 치료에 도움이 될 것으로 기대된다.