• Journal of the Korean GEO-environmental Society
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• v.10 no.1
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• pp.43-48
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• 2009

This study was performed to investigate the characteristics of nutrient removal by Sequencing Batch Reactor (SBR) system, which could achieve high removal efficiencies of nitrogen and phosphorus and make it possible convenient management and operation. In this study, dissolved oxygen (DO), chemical oxygen demand (COD), nitrogen, and phosphorus in SBR system were examined by variation of anoxic-oxic phase repetition in order to optimize an operational method. The 1~4 times of anoxic-oxic phases (Run 1~4) were repeated during 1 cycle operation period. As the repetition frequency increased, it was more difficult to maintain DO condition enough for denitrification. The SBR system showed high COD removal efficiency more than 91% regardless of operational condition. About 68% of nitrogen removal rate was obtained in conditions of 2 or 3 times repetition of anoxic phases, in which NOx-N among discharged total nitrogen account for more than 99%. Approximately 40% of phosphorus was eliminated in the conditions of 1~3 times of anoxic phase repetition.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.10
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• pp.1757-1763
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• 2000

To consider the nutrient removal characteristics of BNR process activating soil microorganisms under the influence of DPB and to clear the characteristics of DPB under anoxic condition was investigated in the this study. The batch tests were conducted using sludge sampled from the BNR process activating soil microorganisms during operation periods. The results of this study were summarized as follows: - The DPB(Denitrifying Phosphorus removing Bacteria) performing denitrification and phosphorus uptake in the anoxic phase plays an important role in removing nitrogen and phosphorus in the BNR process activating soil microorganisms. - The PUR(Phosphorus Uptake Rate) of DPB in the anoxic phase was to be about 50% of PUR in the aerobic phase. - The DPB in the BNR process turned out to be increasing nutrient removal efficiency of BNR process.

• Journal of Korean Society on Water Environment
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• v.20 no.6
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• pp.571-576
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• 2004

Since anaerobic/anoxic/oxic process, which is a typical mainstream biological nitrogen and phosphorus removal process, utilizes influent organic matter as an external carbon source for phosphorus release in anaerobic or anoxic stage, influent COD/T-P ratio gives a strong influence on performance of phosphorus removal process. In this study, a bench scale experiment was carried out for SBR process to investigate nitrogen and phosphorus removal at various influent COD/T-P ratio and nitrate loadings of 23~73 and 1.6~14.3g $NO_3{^-}-N/kg$ MLSS, respectively. The phosphorus release and excess uptake in anoxic condition were very active at influent COD/T-P ratios of 44 and 73. However, its release and uptake was not obviously observed at COD/T-P ratio of 23. Consequently, phosphorus removal efficiency was decreased. In addition, the phosphorus release and uptake rate in anoxic condition increased as the nitrate loading decreased. Specific denitrification rate had significantly high correlation with organic materials and nitrate loadings of the anoxic phase too. The rate of phosphorus release and uptake in the anoxic condition were $0.08{\sim}0.94kg\;S-P/kg\;MLSS{\cdot}d$ and $0.012{\sim}0.1kg\;S-P/kg\;MLSS{\cdot}d$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.3
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• pp.247-253
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• 2003

Sequencing Batch Reactor (SBR) was operated under various experimental conditions to improve the efficiency of biological filters used for the treatment of recycled wastewater from aquaculture. This SBR system was operated for removing COD, ammonia and suspended solid that were the major pollutants in aquaculture wastewater. Aerobic and anoxic conditions after FILL mode were applied intermittently for effective removal of nitrogen. SETTLE and DRAW modes were followed by the complete aerobic and anoxic REACT mode. The total volume of the SBR was 75 liter, while the working volume in a cycle was 35 liters. When the final operating strategy of the SBR was FILL/REACT/SETTLE/DRAW of 0.5/10/1/0.5 hr. the removal efficiencies of TCODcr, $NH_{4}^{+}-N,$ and T-N were 94, 98, and $89\%,$ respectively.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.5
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• pp.861-867
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• 2000

The feasibility of simultaneous phosphorus and nitrogen removal by enhancing anoxic phosphorus uptake was investigated in a sequencing batch reactor (SBR). By introducing an anoxic phase into an anaerobic-aerobic SBR (AO SBR), significant amounts of denitrifying phosphorus accumulating organisms (DPAOs) which can utilize nitrate as electron acceptor could be accumulated in the reactor (anaerobic-aerobic- anoxic-aerobic SBR, $(AO)_2$ SBR). A direct comparison of phosphorus uptake rate under anaerobic and aerobic conditions showed that the fraction of DPAOs in P-removing sludge were increased from 10% in the AO SBR to 64% in $(AO)_2$ SBR. The $(AO)_2$ SBR showed stable phosphorus and nitrogen removal efficiency: average removal efficiencies of TOC, total nitrogen, and phosphorus were 92%, 88%, and 100%. respectively. Results of the $(AO)_2$ SBR operation and batch tests showed that nitrite (up to 10 mg-N/L) was not detrimental to anoxic phosphorus uptake and could serve as good electron acceptor like nitrate.

• Journal of Korean Society on Water Environment
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• v.20 no.5
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• pp.480-487
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• 2004

This study was conducted to find the effect of electron acceptors on the formation of granular sludge by using four different types of electron acceptors. The phosphorous uptake, denitrification, and sulfate reduction in anoxic modes were simultaneously occured because of the presence of the polyphosphate accumultating organism(PAO) that utilize nitrate and sulfate as an electron acceptor in the anoxic zone. Denitrirying phosphorous removal bacteria(DPB) was enriched under anaerobic/anoxic/aerobic condition with a nitrate as an electron acceptor, and desulfating phosphorous removal bacteria(DSPB) was enriched under anaerobic/anoxic/aerobic condition with a sulfate as an electron acceptor. Polyphosphate accumulating organism(PAO) were enriched in the anaerobic/aerobic SBR. PAO took up acetate faster than DPB and DSPB during the aerobic phase. The sludge with nitrate and sulfate as an electron acceptors grew as a granules which possessed high activity and good settleability. In the anaerobic/aerobic modes, typical floccular growth was observed. In the result of bench-scale experiment, simultaneous reactions of phosphorus uptake, denitrification and sulfate reduction were observed under anoxic condition with nitrate and sulfate as an electron acceptors. These results demonstrated that the anaerobic/anoxic modes with nitrate and sulfate as an electron acceptors played an important role in the formation of the sludge granulation.

• Journal of Korean Society of Water and Wastewater
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• v.21 no.4
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• pp.409-420
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• 2007

ASM No. 1 is a very useful model to analyze wastewater treatment system removing organic carbon and nitrogen material. But it isn't adequate to control the wastewater treatment system with real time since it has many material divisions and parameters. So, the purpose of this study is the simplification of ASM No. 1 to control the wastewater treatment system. ASM No. 1 was changed with the model which has 3 material divisions(COD, $NH_4{^+}$, $NO_3{^-}$) and two phases(Aerobic and Anoxic condition). SBR was running with two phases(Phase I and II). Phase II running 20 minutes with aerobic time was used for deciding model parameters and Phase I running 12 minutes with aerobic time was used for proving the simplified model. The simplified model was compared with ASM No. 1 using data of Phase I and II. As a result of model comparison, the simplified model has enough ability to express the variation of $NH_4{^+}$ compound.

• Journal of Microbiology and Biotechnology
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• v.23 no.1
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• pp.99-105
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• 2013

In this study, nitrous oxide ($N_2O$) emission was compared between the operations of two different sequencing batch reactors, conventional sequencing batch reactor (CNVSBR) and simultaneous nitrification and denitrification sequencing batch reactor (SND-SBR), using synthetic wastewater. The CNV-SBR consisted of anoxic (denitrification) and aerobic phases, whereas the SND-SBR consisted of a microaerobic (low dissolved oxygen concentration) phase, which was achieved by intermittent aeration for simultaneous nitrification and denitrification. The CNV-SBR emitted 3.9 mg of $N_2O$-N in the denitrification phase and 1.6 mg of $N_2O$-N in the nitrification phase, resulting in a total emission of 5.5mg from 432mg of $NH_4^+$-N input. In contrast, the SND-SBR emitted 26.2mg of $N_2O$-N under the microaerobic condition, which was about 5 times higher than the emission obtained with the CNV-SBR at the same $NH_4^+$-N input. From the $N_2O$ yield based on $NH_4^+$-N input, the microaerobic condition produced the highest yield (6.1%), followed by the anoxic (0.9%) and aerobic (0.4%) conditions. It is thought that an appropriate dissolved oxygen level is critical for reducing $N_2O$ emission during nitrification and denitrification at wastewater treatment plants.

• Journal of Environmental Science International
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• v.6 no.6
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• pp.697-709
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• 1997

Sequencing Batch Reactor(SBR) experiments for organics and nutrients removal have been conducted to find an optimum anaerobic/anoxic/aerobic cycling time and evaluate the applicability of oxidation-reduction potential(ORP) as a process control parameter. In this study, a 61 bench-scale plant was used and fed with night-soil wastewater in K city which contained TCODcr : 10, 680 mg/l, TBm : 6, 893 mg/l, $NH_4^+-N$ : 1, 609 mg/l, $PO_4^{3-}-P$ : 602 mg/l on average. The cycling time In SBRs was adjusted at 12 hours and 24 hours, and then certainly included anaerobic, aerobic and inoxic conditions. Also, for each cycling time, we performed 3 series of experiment simultaneously which was set up 10 days, 20 days and 30 days as SRT From the experimental results, the optimum cycling time for biological nutrient removal with nlght-soil wastewater was respctively 3hrs, 5hrs, 3hrs(anaerobic-aerobic-anoxic), Nitrogen removal efficiency was 77.9%, 77.9%, 81.7% for each SRT, respectively. When external carbon source was fed in the anoxic phase, ORP-bending point indicating nitrate break point appeared clearly and nitrogen removal efficiency increased as 96.5%, 97.1%, 98.9%. Phosphate removal efficiency was 59.8%, 64.571, 68.6% for each SRT. Also, we finded the applicability of ORP as a process control parameter in SBRs.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2115-2120
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• 2012

The presence of lung cancer cells in anoxic zones is a key cause od chemotherapeutic resistance. Thus, it is necessary to enhance the sensitivity of such lung cancer cells. However, loss of efficient gene therapeutic targeting and inefficient objective gene expression in the anoxic zone in lung cancer are dilemmas. In the present study, a eukaryotic expression plasmid pUC57-HRE-JAB1 driven by a hypoxia response elements promoter was constructed and introduced into lung cancer cell line A549. The cells were then exposed to a chemotherapeutic drug cis-diamminedichloroplatinum (C-DDP). qRT-PCR and western blotting were used to determine the mRNA and protein level and flow cytometry to examine the cell cycle and apoptosis of A549 transfected pUC57-HRE-JAB1. The results showed that JAB1 gene in the A549 was overexpressed after the transfection, cell proliferation being arrested in G1 phase and the apoptosis ratio significantly increased. Importantly, introduction of pUC57-HRE-JAB1 significantly increased the chemotherapeutic sensitivity of A549 in an anoxic environment. In conclusion, JAB1 overexpression might provide a novel strategy to overcome chemotherapeutic resistance in lung cancer.