• 대한한방내과학회지
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• 제29권1호
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• pp.200-218
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• 2008

Objectives : The purpose of this study was to investigate the effects of Injinchunggan-tang (Yinchenchinggan-tang) on DMN-induced liver damage by applying proteomics. Materials and Methods : Sprague-Dawley rats were used in this experiment and were divided into the normal group (normal saline), the control group (DMN) and the sample group (DMN+IJCGT). DMN was injected i.p. once a day three times a week for 3 weeks in the control group. Normal saline instead of DMN was administered to the normal group. In the sample group, Injinchunggan-tang (Yinchenchinggan-tang) extract was orally administered once a day for 10 days after DMN was induced. The livers of each group were processed and analyzed by histology, Western blot, $Oxyblot^{TM}$, CBB and 2-dimensional electrophoresis. Results : In the histological findings of the liver, IJCGT reduced collagen deposition and liver damage in DMN-induced hepatic fibrosis. IJCGT increased MMP-13 protein production assessed by western blot. Protein oxidation induced by DMN treatment was decreased by IJCGT. In the 2-dimensional electrophoresis finding, the level of the increased proteins induced by DMN treatment such as GRP 75, 58kDa glucose regulated protein and heat shock 70kDa protein 5 were decreased by IJCGT. IJCGT was considered to have the protective effects on hepatotoxicity induced by DMN. In the 2-dimensional electrophoresis finding, the level of increased oxidized proteins such as heat shock 70 protein, mitochondrial malonyltransferase, calreticulin precursor, actin, NADP-isocitrate dehydrogenase, ankyrin repeat and SOCS box protein 11 were decreased by IJCGT. IJCGT was considered to have protective effect on the protein production induced by DMN treatment. Conclusion : Injinchunggan-tang (Yinchenchinggan-tang) exerts an inhibitory effect against the fibrosis and protein oxidation induced by DMN treatment in the rat liver. IJCGT was considered to have protective effects on the hepatotoxicity and protein production induced by DMN treatment.

• Asian-Australasian Journal of Animal Sciences
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• 제32권4호
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• pp.494-500
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• 2019

Objective: Feed consumption contributes a large percentage for total production costs in the poultry industry. Detecting genes associated with feeding traits will be of benefit to improve our understanding of the molecular determinants for feed efficiency. The objective of this study was to identify candidate genes associated with feed conversion ratio (FCR) via genomewide association study (GWAS) using sequence data imputed from single nucleotide polymorphism (SNP) panel in a Chinese indigenous chicken population. Methods: A total of 435 Chinese indigenous chickens were phenotyped for FCR and were genotyped using a 600K SNP genotyping array. Twenty-four birds were selected for sequencing, and the 600K SNP panel data were imputed to whole sequence data with the 24 birds as the reference. The GWAS were performed with GEMMA software. Results: After quality control, 8,626,020 SNPs were used for sequence based GWAS, in which ten significant genomic regions were detected to be associated with FCR. Ten candidate genes, ubiquitin specific peptidase 44, leukotriene A4 hydrolase, ETS transcription factor, R-spondin 2, inhibitor of apoptosis protein 3, sosondowah ankyrin repeat domain family member D, calmodulin regulated spectrin associated protein family member 2, zinc finger and BTB domain containing 41, potassium sodium-activated channel subfamily T member 2, and member of RAS oncogene family were annotated. Several of them were within or near the reported FCR quantitative trait loci, and others were newly reported. Conclusion: Results from this study provide valuable prior information on chicken genomic breeding programs, and potentially improve our understanding of the molecular mechanism for feeding traits.

• 한국국제농업개발학회지
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• 제23권5호
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• pp.537-545
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• 2011

본 연구는 간척지에서 재배가 가능한 내염성 보리 품종육성을 위한 기초정보를 얻고자 겉보리 두 품종을 대상으로 생육 초기 염 스트레스에 따른 생리적 반응과 잎 프로테옴의 발현 양상 변화를 분석한 결과는 아래와 같다. 1. 토양의 염 농도가 증가함에 따라 보리의 건물중은 무처리구에 비해 유의적으로 감소하는 경향이었으며, 상록보리는 무처리구에 비해 건물중 감소가 작았으며, 선우보리는 컸다. 2. 염처리에 따른 잎의 엽록소 함량을 나타내는 SPAD 값은 상록보리가 57.6으로 47.6인 선우보리보다 높았으며, Na⁺의 함량은 선우보리에서 유의적으로 높았고, K⁺/Na⁺의 비율은 상록 보리에서 높은 경향을 보였다. 3. 이차원전기영동에 의하여 염 스트레스에 의한 잎 프로테옴의 발현양상을 분석한 결과 47개 단백질 spot이 발현양의 차이를 나타냈다. 품종별로 발현양이 증가한 단백질 spot은 상록보리와 선우보리에서 각각 17개와 14개로 나타났고, 발현양이 감소한 단백질 spot은 상록보리와 선우보리에서 각각 28개 및 27개로 확인되었다. 4. 염처리에 따른 발현양의 차이를 보이는 18개 단백질을 동정한 결과 ribosomal protein 등 기능과 스트레스와의 관련성이 보고된 10개의 단백질과 ankyrin repeat domain protein 등 스트레스 조건에서의 역할이 명확하지 않은 4개의 단백질 및 Os02g0753300 등 기능 및 스트레스와의 관련성을 알 수 없는 2개의 단백질이 동정되었다.