• Journal of Environmental Health Sciences
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• v.23 no.1
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• pp.14-17
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• 1997

A total of 93 specimens of Astroconger myriaster were purchased from the Chungmu fishery market at the south sea area during the period from October 1 to November 25, 1996. Specimens were examined for the infection status with larvae anisakid. Anisakid larvae were collected from viscera, muscle, omenturn and liver. Eight hundred four larval anisakids sorted from 93 specimens of A. myriaster ranged from 9.0 to 26.5mm in their body length. They were classified based on morphological and morphometric observations as follows Anisakis type 1 larvae (465 larvae, 57.8%: positive rate), Contracaecum type D(129, 16.0%), Contracaecum type A(90, 11.2%), Anisakis type 11(60, 7.5%), Phapidascaris(3, 0.4%) and unknown type(57, 7.1%).

• Korean Journal of Environmental Biology
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• v.21 no.2
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• pp.177-180
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• 2003

Four genera of metazoan parasites were recovered from 3 of 6 Sebastes pinniger examined during January 2003. Two hundred forty-five metazoan parasites were recovered. Trochopus trituba Sekerak and Arai, 1977 and Anisakis larvae were found to he infected S. finniger Gill and te be abundant in this rockfish. One hundred eleven worms of monogenean trematodes and 134 larval anisakine nematodes were also found in this fishes. Precise positions with regard to gills, fins, and gastrointestinal tracts were recorded on S. pinniger to determine distribution of metazoan parasites of Trochopus trituba, Anisakis simplex larva, Contracaecum sp. larva, and Pseudo terranova decipiens larva. The monogenean trematodes, T. trituba were attacked to the gill arches and fin, and the larval anisakine nematodes, A. simplex, Contracaecum sp.,and P. decipiens were found on the visceral organs such as intestine, omentum and stomach wall. The second gill arches were the most heavily infested and posterodorsal region of the gill arches contained most monegenenn trematodes. The nam site of infestation of larval anisakine nematodes were the intestine.

• Parasites, Hosts and Diseases
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• v.41 no.2
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• pp.97-100
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• 2003

Acute gastric anisakiasis with multiple anisakid larvae infection is reported. A 68-year-old woman residing in Busan, Korea, had epigastric pain with severe vomiting about 5 hours after eating raw anchovies. Four nematode larvae penetrating the gastric mucosae in the great curvature of the middle body and fundus were found and removed during gasteroendoscopic examination. Another one thread-like moving larva was found in the great curvature of upper body on the following day. On the basis of their morphology, the worms were identified as the 3rd stage larvae of Anisakis simplex. This case is acute gastric anisakiasis provoking severe clinical problems by the multiple infection and the greatest number of anisakid larvae found in a patient in Korea.

• Korean Journal of Environmental Biology
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• v.20 no.4
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• pp.309-311
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• 2002

A total of ten specimens of darkblotched rockfish, Sebastes crameri obtain-ed from Newport fish market were examined for parasites during February, 2002. Eight out of ten S. crameri were found to be infected with metazoan parasites. They were flatworm Trochopus australis, nematoda Anisakis simplex, and copepods Neobrachiella robusta and Chondracanthus triventricosus. Nine (30.0%) T. australis, seven (30.0%) N. robusta, one (10.0％) C. triventricosus and seven (60.0%) A. simplex were infected. Among them, A. simplex was the most abundant and prevalent metazoan parasites in S. crameri. T. australis and N. robsta were found in gills with moderate intensity (1.7 and 1.9, respectively), and C. triventricosus in dorsal fin with low intensity (0.3).

• Journal of Environmental Health Sciences
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• v.26 no.2
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• pp.67-69
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• 2000

Yellow tails (Seriola quinqueradiata) were purchased from the Jakalchi fish market located in Busna and examined for anisakid larvae. The collected larvae were classified by their morphologicaltypes. Of the 16 yellow tails exmained, seven (43.8%) were infected with the larvae. Among 90 larvae, 81 were collected from the omentum and 9 from the muscle. Anisakids were classified into five larval types, anisakis type I (56 larvae in number, 62.2%), Contracaecum type A (8, 8.9%) and type D (7, 7.8%) and Contracaecum type C (19, 21.1%). The present study revealed that Anisakis type I was the most common among the five larval types in the yellow tails caught in the South sea of Korea peninsula.

• Korean Journal of Veterinary Research
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• v.21 no.1
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• pp.7-10
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• 1981

During the studies on the esophagogastric ulcers in swine, Anisakis type larvae were found associated with stomach lesions. 1. Of the 1,531 pigs examined, 11(0.7%) were infested with the parasite in the stomach. 2. The parasite was identified as Anisakis type I larvae, according to the morphological characteristics. 3. On gross examination, the parasites were seen penetrating gastric mucosa, resulting in hemorrhagic ulcers. 4. Main histopathological features were edema, massive eosinophilic infiltrations around the parasite, necrosis and perivascular eosinophilic accumulations in the submucosa.

• Korean Journal of Veterinary Research
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• v.21 no.1
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• pp.45-50
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• 1981

In the survey on the pig-stomach worms of the abattoir pigs in Gyeongbug province, some unusual nematodes were found by gross and microscopic inspection to the pig-stomach. The results obtained in the survey and identification were summarized as follows. 1. A natural infection of Anisakis type larvae in the pig gastric wall was newly confirmed in korea and in all cases, the larvae were identified as Anisakis type 1 (Berland, 1961). 2. A chronic granulomatous lesion with small lymphocytes, neutrophils and eosinophils was appeared in the gastric wall penetrated by the larvae. 3. Of total 318 pigs, 9 pigs were infected with the larvae and in each stomach, only one or two worms were detected from the abattoir pigs. 4. Ascarops strongylina was found as one percent.

• Journal of fish pathology
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• v.23 no.3
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• pp.421-427
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• 2010

Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon (Oncorhynchus masou). Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.

• Parasites, Hosts and Diseases
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• v.33 no.2
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• pp.95-100
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• 1995

A survey was carried out on the larval anilsakids in anchovies (Engrnuyis jnponica) which were caught from April to .lune in 1993 at the southern and eastern sea in Korea. Anisakids in anchovies were morphological classified into Anisakis type I and II and Contracaecum type B and C, after fixation in 70% alcohol and clearing in lactophenol. A examined. The infection rates showed no relation with length of the anchovies. The numbers of anisakids by organs of anchovies were 96 (56.1%) from the muscle, 65 (38.0%) from the omentum, 7 (4.1%) from the intestine, 2 (1.7%) from the stomach and 1 (0.6%) from the testis. Infection rates of anchovies with anisakids by localities were 5.1% at Taebyon, 8.0% at Sokcho and 9.2% at Chungmu. The larval uisikids in anchovies may infect humans who often eat raw anchovies.

• Parasites, Hosts and Diseases
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• v.50 no.2
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• pp.165-171
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• 2012

Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as ${\alpha}$-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-$40^{\circ}C$) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.