• Asian-Australasian Journal of Animal Sciences
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• 제21권1호
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• pp.120-123
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• 2008

This experiment was conducted to investigate the effect of different nipple angles on water disappearance in growing pigs, which is defined as the water which leaves the watering device but is not consumed by the pig. This water adds to the volume of the total waste slurry. Four crossbred pigs averaging $70.0{\pm}1.4$ kg were assigned into 1 of 4 treatments using a $4{\times}4$ Latin-square design. Treatments were distinguished by the angle between nipple terminal and the perpendicular wall. These angles were: 1) NA-30 (= Nipple Angle $30^{\circ}$), 2) NA-45 (= Nipple Angle $45^{\circ}$), 3) NA-60 (= Nipple Angle $60^{\circ}$) and 4) NA-90 (= Nipple Angle $90^{\circ}$). All of the nipples were fixed at shoulder height of each pig. After a 7-day adaptation period, samples were collected from each pig for 4 days, followed by a day for change-over. Pigs were fed a 0.8 kg diet twice daily at 08:00 and 20:00 and supplied water ad libitum. Throughout the experimental period, pigs in the NA-30 treatment group showed greater water disappearance than other treatments. Water disappearance was the lowest for the NA-60 treatment group (p<0.01). The percentage of water disappearance to the water supply was significantly reduced in the NA-60 treatment group (p<0.01). Water intake was the same for all the treatment groups. The rate of water disappearance per unit of water intake was significantly decreased in pigs in the NA-60 treatment group compared to other groups (p<0.01). These results suggested that the nipple angle for growing pigs should be $60^{\circ}$ to reduce water disappearance and, subsequently, the amount of waste generated.

• Asian-Australasian Journal of Animal Sciences
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• 제33권12호
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• pp.1999-2007
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• 2020

Objective: This study was aimed to investigate the muscle-specific beef color stability at normal and high ultimate pHs. Methods: The impact of muscle (Longissimus lumborum [LL] vs psoas major [PM]) and pH (normal ultimate pH [Np] vs high pH dark cutting beef [Hp]) on color stability, indicated by basic color traits, metmyoglobin reducing activity (MRA) and oxygen consumption (OC), as well as the lipid oxidation, were determined over 7 days of display at 4℃. Results: Hp-LL had the highest pH (6.92), followed by Hp-PM (6.01), Np-PM (5.76), and Np-LL (5.52). Hp-LL had increased (p<0.05) a^⋆, chroma and % oxymyoglobin during display. Hp-LL also had the highest metmyoglobin (MMb) reducing activity and OC among all the samples, thus, the greatest color stability, although very dark throughout storage, with lowest values for lightness (L^⋆) and yellowness (b^⋆). Np-LL also exhibited relatively high color stability, as a result of its lower % MMb and OC and higher MRA than psoas muscle samples. The 0.2 unit difference of the pH between Hp and Np psoas muscle, resulted in the difference of the color intensity, not the color stability. Interestingly, high pH psoas muscle (Hp-PM) did not have better color stability than Np-PM, and in fact had lower color stability than even Np-LL. The similar level of OC and lipid oxidation cannot explain the difference in color stability between Hp-PM and Np-LL. Conclusion: The Hp does not always show better color stability compared with Np beef, which depends on the muscle type. The balance of MRA and OC is important to keep the color in great intensity and stability in the meantime.

• Asian-Australasian Journal of Animal Sciences
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• 제19권3호
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• pp.444-449
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• 2006

This study investigated the effects of dietary supplementation of cheese byproduct on performance, egg quality and fatty acid profile of egg yolk lipids from laying hens. One hundred five 30-wk-old White leghorn laying hens were randomly distributed into five groups of twenty one hens each and maintained in individual laying cages for 4 weeks. The hens were assigned to five treatments that consisted of corn-soybean meal based diets containing 0, 1, 3, 5 or 10% of cheese byproduct. Feed intake and rate of egg production of hens were not significantly different across the treatments during the whole experiment (p>0.05). Similarly, egg yolk cholesterol level, egg weight, Haugh's unit, eggshell thickness, color, and strength were not significantly different across the treatments (p>0.05). The amount of C16:0 in egg yolk was not significantly different across the treatments, but that of C18:0 decreased with increased cheese byproduct (p<0.01). Monounsaturated fatty acid (C16:1 and C18:1) content in egg yolk was similar across the treatments. Total CLA and cis-9, trans-11 CLA content increased linearly with increased cheese byproduct (p<0.001), while trans-10, cis-12 CLA amount was not significantly different across the treatments (p>0.05). Total saturated fatty acid (SFA) in the egg yolk was decreased as the level of cheese byproduct including CLA increased (p<0.01). However, the amount of unsaturated fatty acids (UFA) such as monounsaturated fatty acids (MUFA), n-3 polyunsaturated fatty acids (PUFA), n-6 PUFA, and total PUFAs in the egg yolk were not significantly different across the treatments (p>0.05). Therefore, the present results showed that cheese byproduct beneficially improved the fatty acid composition of concern to human health in the egg yolk without adverse effects on egg quality.

• 한국임상수의학회지
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• 제18권4호
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• pp.329-333
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• 2001

새로 개발되거나 혹은 기존의 어떤 진단검사를 다양한 임상상황에 적용하기 위해서는 먼저 이들 검사법의 진단적 정확도를 추정하는 연구가 반드시 선행되어야 한다. 진단의 정확도에 대한 추정치를 모른다면 검사결과를 해석하는 것이 불가능하기 때문이다. 특히 동일한 개체에서 감염부위별로 3개 이상의 시료를 얻어 진단검사를 적용하는 경우 각 시료의 검사결과는 독립적인 측정시료가 아니라 개체내에서 연관성이 매우 높은 종속적인 시료에 해당 한다. 즉 동일한 개체에서 얻은 시료일수록 검사결과에서 유사한 반응을 보이며 이 경우 분석의 단위는 각각의 개체가 아니라 검사부위가 되는데 이는 의학연구에서 매우 흔하다. 본 연구에서는 Helicobacter 균에 의한 감염을 검출하기 위하여 동일한 개로부터 위의 해부학적 구조상 pyloric antrum, body 및 cardia의 생검시료에 대하여 urease 검사와 PCR 검사를 적용하여 각 검사의 진단적 정확도를 추정하였다. urease 검사의 민감도와 특이도는 0.74 (95% 신뢰구간: 0.64-0.84)와 0.87 (95% 신뢰구간: 0.67-1.00)이었으며 PCR 검사의 민감도와 특이도는 0.95 (95% 신뢰구간: 0.89-1.00)와 0.90 (95% 신뢰구간: 0.70-1.00)로 두 검사의 특이도는 높은 것으로 나타났다. 그러나 PCR 검사에 비하여 urease 검사의 경우 가음성 (false negative)의 가능성이 높기 때문에 진단결과에 대한 신중한 해석이 요구된다.

• 한국가금학회지
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• 제32권4호
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• pp.275-279
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• 2005

본 시험은 시중에 유통되고 있는 일반 건조 잔반을 AO 배양물로서 산란계에 급여하여, 산란계의 생산성과 계란 품질에 미치는 영향을 구명하고자 실시하였다. 시험축은 평균 체중이 $1.5\pm0.5kg$인 50주령 산란계(ISA-Brown)를 공시하였고, 기초사료로 산란계 중기 사료를 급여하였으며, 시험사료는 대조구(Control), 잔반사료(FW)와 AO 배양물사료(AFW)를 각각 $25\%,\;50\%,\;75\%$씩 기초사료를 대체하여 총 7처리구, 3반복, 반복당 8수씩 168수를 공시하였다. 시험기간 중 사료섭취량에서 전처리구간 차이는 없었으나, FW구의 $50\%$ 처리구에서 대조구에 비해 유의적으로 감소했고, 산란률에 있어서는 대조구 $89.8\%$에 비해 AFW $25\%$ 처리구에서는 차이가 없었으나, 전 처리구에서 유의적으로 낮았다(p<0.05). 음수량은 잔반 사료의 대체 수준에 따라 증가하였다(p<0.05). 난중, 난각 두께 및 난각 강도는 전 처리구간 차이가 없었다. 호우유니트와 난황색에서는 남은 음식물 사료의 첨가에 따라 뚜렷한 차이를 보였으나(p<0.05) $50\%$ 이상의 첨가에서는 차이가 없었다. 남은 음식물사료의 첨가에 따른 난황내 콜레스테롤 함량에는 전 처리구간 차이가 없었다. 이러한 결과로부터, 잔반 사료는 산란율을 떨어뜨리지만, 난질 개선에 효과가 있고, 산란계 사료의 첨가제로서 AO 접종 잔반 사료의 사용이 가능할 수 있음을 시사한다.

• 생명과학회지
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• 제23권2호
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• pp.237-241
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• 2013

산란계 사료 내 유색보리 혹은 청보리의 10 또는 20% 첨가가 산란계의 생산성과 계란 품질에 미치는 영향을 조사하기 위하여 총 200 마리의 Brown nick을 5처리구에 5반복으로 배치하며 8주간 사양하였다. 시험군의 사료섭취량, 산란율, 난중과 일산란량은 대조구와 유의성 있게 달랐으며 특히 사료섭취량, 산란량과 일산란량은 보리의 함량이 증가할수록 유의적으로 감소하였다. 계란의 품질 중 난황색, 난각강도 또는 난각색은 사양 6주차까지의 보리 시험군에서 유의하였으나 보리를 8주차까지 급이한다면 난백고와 더불어 호우유닛에 긍정적인 영향을 나타내었다. 사양 6주까지에 대한 보리의 급이는 난각색과 난황색의 수치를 첨가량에 따라 유의적으로 높인 반면 난각강도에 대한 유의성은 없었다. 산란계 사료 내 보리의 함량을 20%로 증가시켜 급이한다면 난백고와 호우유닛은 유의적으로 증가하였으며 특히, 유색보리보다는 청보리의 첨가군에서 수치적으로 높은 9.10과 94.53의 난백고와 호우유닛을 나타내었다. 따라서 본 연구에서는 산란계의 생산성과 계란의 품질을 고려한 보리 첨가 산란계 사료를 생산하고자 한다면 생산성에서는 대조구와 유사하면서도 계란의 품질을 향상시킨 청보리 혹은 유색보리를 최대 10%까지 첨가한 산란계 사료의 생산을 고려해 볼 수 있을 것으로 판단된다.

• 한국축산시설환경학회지
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• 제5권3호
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• pp.157-170
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• 1999

This study was conducted to investigate the effects of Scoria, Quartz porphyry and Oak charcoal powder feeding by 1% level of concentrate on the fattening cross bred bulls(5/8 Korean Native Cattle, 2/8 Charolais, 1/8 Brahman germ plasma) Feeding trial was conducted with 4 treatment groups which were T1(Full feeding of concentrate and roughage for 12 months), T2(T1 and Scoria addition), T3(T1 and Quartz porphyry addition) and T4(Oak charcoal addition) for 360 days, consisting of 32 heads whose initially weights were about 299.7kg. The results obtained are summarized as follows; 1. During the over-all period, average daily gains by T3, T4, T2 and T1 were 1.024, 0.987, 0.977kg and 0.964, respectively, without a significant difference by treatments. 2. Concentrate intakes per body weight and TDN intakes required per unit of kilogram gain were lower in addition agent groups than in control, and in all the treatments feed efficiency was higher at early stage for 360 fattening day than at end stage. 3. Carcass weight, dressing percent, back-fat thickness and loin eye muscle area by treatments were 330.9 to 348.4kg(average 340.3kg), 55.2~56.2(average 55.8%), 0.41~0.55(average 0.46cm)and 76.0~80.9$\textrm{cm}^2$(average 77.6$\textrm{cm}^2$), respectively, and were improved in addition agent groups than in control. 4. Meat quality grade is higher in addition agent groups than in control, and heads of 1st grade by treatments was 3, 2, 1 and none in T4, T2, T3 and T1, respectively. 5. Incidence heads of diseases by treatment were not apparently different, but diseases of digestive system was decreased in addition agent groups than in control. 6. The nitrate nitrogen content of fattening bull dung which collected in rectum were 0.082~0.089% (average 0.084%), and the content in addition agent groups was smaller than in controll. 7. Income was higher in order of T4, T3, T2, and T1 and incomes of treatment groups were grater than that of control group by 21.4 to 33.5 percent. According to the above results it may be concluded that fattening bulls may be required to feed no more than 1% of Scoria, Quartz porphyry and Oak charcoal powder based on the concentrate feeding level during the fattening period(12 to 24 month of age) to produce high quality meat and increase income.

• 한국축산식품학회지
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• 제29권3호
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• pp.340-348
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• 2009

본 연구는 비육한우 50두를 공시하여 일반사료를 급여한 대조구와 야생쑥 2%를 급여한 시험구 등 2개 처리구로 나누어 생후14개월-29개월령까지 14.7개월간 비육시험하고, 생산된 고기의 육질을 분석하였다. 쑥사료를 급여한 거세한우의 생산성에서 총증체량, 일당증체량이 대조구보다 처리구에서 높았고, 사료요구율이 낮아 쑥사료를 급여함으로 사료효율이 개선되었다. 혈중 총 콜레스테롤은 대체로 정상범위로서 비육전기보다 비육후기가 더 높은 경향이었고, 비육이 진행될수록 증가하는 경향을 나타내었으며, GOT와 GPT 및 BUN은 처리구간의 유의성은 없었다. 도체중은 처리구가 대조구보다 높았고, 매최장근단면적은 처리구가 대조구보다 넓었다. 육량 및 육질등급을 환산하였을 때 처리구(각 1.80점, 2.39점)가 대조구(1.70점, 2.30점)보다 높은 결과이었다. 콜레스테롤 함량에서 쑥이 첨가된 처리구가 대조구보다 유의적으로 낮게 나타났다(p<0.05). 가열감량 및 육색의 $L^*$, $a^*$, $b^*$, chroma 및 hue 값은 처리구간의 통계적인 유의차는 없었으며, 저장기간이 경과할수록 점차 낮아지는 경향이었다. 지방산에서 포화지방산은 대조구가 시험구보다 크게 높았고, 불포화지방산은 오히려 시험구가 대조구보다 유의적으로 높게 나타났다(p<0.05). 이상의 결과를 종합해 볼 때, 거세한우에게 쑥을 2% 첨가, 급여하면 콜레스테롤 함량이 낮으며, 불포화지방산 함량이 높은 우육을 생산할 수 있을 것으로 생각된다.

• Animal Bioscience
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• 제34권6호
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• pp.1029-1037
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• 2021

Objective: This study was conducted to investigate effects of antifungal substance and carboxylesterase-producing inoculant on fermentation indices and rumen degradation kinetics of whole crop rice (WCR) silage ensiled at different dry matter (DM) contents. Methods: Dual-purpose inoculants, Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1, confirmed both activities of antifungal and carboxylesterase in the previous study. The WCR at mature stage was chopped, and then wilted to obtain three different DM contents consisting of 35.4%, 43.6%, and 51.5%. All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1×105 colony forming unit/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d. Results: The INO silages had lower lactate (p<0.001) and butyrate (p = 0.022) with higher acetate (p<0.001) and propionate (p<0.001) than those of CON silages. Ammonia-N (p<0.001), lactate (tendency; p = 0.068), acetate (p = 0.030), and butyrate (p<0.001) concentrations of INO silages decreased linearly with increasing DM content of WCR forage. The INO silages presented higher lactic acid bacteria (p<0.001) with lower molds (p<0.001) than those of CON silages. Yeasts (p = 0.042) and molds (p = 0.046) of WCR silages decreased linearly with increasing DM content of WCR forage. In the rumen, INO silages had higher the total degradable fraction (p<0.001), total volatile fatty acid (tendency; p = 0.097), and acetate (p = 0.007), but lower the fractional degradation rate (p = 0.011) and propionate (p<0.001) than those of CON silage. The total degradable fraction (p<0.001), total volatile fatty acid (p = 0.001), iso-butyrate (p = 0.036), and valerate (p = 0.008) decreased linearly with increasing DM content of WCR forage, while the lag phase (p<0.001) was increased linearly. Conclusion: This study concluded that application of dual-purpose inoculants on WCR silage confirmed antifungal and carboxylesterase activities by inhibiting mold and improving rumen digestibility, while increase of wilting times decreased organic acids production and rumen digestibility.

• Asian-Australasian Journal of Animal Sciences
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• 제32권10호
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• pp.1528-1539
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• 2019

Objective: To evaluate the effects on microbial diversity and biochemical parameters of gradually increasing temperatures, from $5^{\circ}C$ to $25^{\circ}C$ on corn silage which was previously fermented at ambient or low temperature. Methods: Whole-plant corn silage was fermented in vacuum bag mini-silos at either $10^{\circ}C$ or $20^{\circ}C$ for two months and stored at $5^{\circ}C$ for two months. The mini-silos were then subjected to additional incubation from $5^{\circ}C$ to $25^{\circ}C$ in $5^{\circ}C$ increments. Bacterial and fungal diversity was assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiling and biochemical analysis from mini-silos collected at each temperature. Results: A temperature of $10^{\circ}C$ during fermentation restricted silage fermentation compared to fermentation temperature of $20^{\circ}C$. As storage temperature increased from $5^{\circ}C$ to $25^{\circ}C$, little changes occurred in silages fermented at $20^{\circ}C$, in terms of most biochemical parameters as well as bacterial and fungal populations. However, a high number of enterobacteria and yeasts (4 to $5\;log_{10}$ colony forming unit/g fresh materials) were detected at $15^{\circ}C$ and above. PCR-DGGE profile showed that Candida humilis predominated the fungi flora. For silage fermented at $10^{\circ}C$, no significant changes were observed in most silage characteristics when temperature was increased from $5^{\circ}C$ to $20^{\circ}C$. However, above $20^{\circ}C$, silage fermentation resumed as observed from the significantly increased number of lactic acid bacteria colonies, acetic acid content, and the rapid decline in pH and water-soluble carbohydrates concentration. DGGE results showed that Lactobacillus buchneri started to dominate the bacterial flora as temperature increased from $20^{\circ}C$ to $25^{\circ}C$. Conclusion: Temperature during fermentation as well as temperature during storage modulates microorganism population development and fermentation patterns. Silage fermented at $20^{\circ}C$ indicated that these silages should have lower aerobic stability at opening because of better survival of yeasts and enterobacteria.