• Korean Journal of Poultry Science
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• v.32 no.2
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• pp.107-112
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• 2005

This study was conducted to establish a comprehensive grading guideline for poultry. Guidelines for sampling size and grading were determined on the basis of survey data obtained from chicken processing house. Results were summarized as follows : For application unit, most chicken processing house preferred lot unit to farm unit. Most processing house did not want the total survey method, but selection sampling. At the beginning of the grading system, chicken processing house wanted to apply the system to a particular lots for which the company requests. There were much suggestions for grading not only portion meat, but also whole chicken. Bigger processing house preferred no. $8\~12$ to be graded. Most of large-scale processing houses used more than 81 volts for stunning and cooling tanks by soaking. For shipping chicken, Large-scale processing houses used both individual and box packing while small ones only used box packing.

• Journal of Veterinary Science
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• v.23 no.3
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• pp.33.1-33.10
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• 2022

Background: Endemic circulation of human-specific hepatitis E virus (HEV) genotypes 1 and 2 may occult the importance of sporadic zoonotic HEV transmissions in Africa. Increasing numbers of studies reporting anti-HEV antibodies in cattle and the discovery of infectious HEV in cow milk has raised public health concern, but cattle exposure has seldom been investigated in Africa. Objectives: This study aimed at investigating the role of cows in the epidemiology of HEV in Burkina Faso and farmers habits in terms of dairy product consumption as a prerequisite to estimate the risk of transmission to humans. Methods: Sera from 475 cattle and 192 pigs were screened for the presence of anti-HEV antibodies while HEV RNA in swine stools was detected by reverse transcription polymerase chain reaction. Data on mixed farming, dairy product consumption and selling habits were gathered through questionnaires. Results: The overall seroprevalence in cattle was 5.1% and herd seroprevalence reached 32.4% (11/34). Herd seropositivity was not associated with husbandry practice or presence of rabbits on the farms. However, herd seropositivity was associated with on-site presence of pigs, 80.7% of which had anti-HEV antibodies. The majority of farmers reported to preferentially consume raw milk based dairy products. Conclusions: Concomitant presence of pigs on cattle farms constitutes a risk factor for HEV exposure of cattle. However, the risk of HEV infections associated with raw cow dairy product consumption is currently considered as low.

• Journal of Nutrition and Health
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• v.55 no.2
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• pp.201-210
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• 2022

Vitamin A (Vit A) is a lipid-soluble vitamin required for diverse normal body functions, including good vision, reproduction, growth, development, and cellular differentiation. The therapeutic effects of Vit A have been demonstrated for the treatments of inflammation, low immunity, and cancer. The present review discusses the scientific evidence for establishing the 2020 Dietary Reference Intakes for Koreans (KDRI) for Vit A, issues caused by unit change of Vit A, and suggestions for the 2025 KDRI revision. Due to the changes in the standard bodyweight observed in several age groups, the 2020 KDRI had minor revisions as compared to the 2015 KDRI. In the 2015 KDRI, the Vit A unit has changed from retinol equivalent (RE) to retinol activity equivalent (RAE) and the activity of carotenoids became half with RAE compared to RE due to this unit change. Since the Vit A intake of Koreans relies heavily on plant-based carotenoids, the dietary intake of Vit A in Koreans as determined by considering the RAE was much lower than values obtained with RE. The analysis for Vit A intake by the Korean National Health and Nutrition Survey only reflects intakes of retinol and beta-carotene. Thus, it would be necessary to include the consumption of other provitamin A, such as alpha-carotene and beta-cryptoxanthin. Moreover, assessing the amounts of Vit A in foods should be customized to Korean diets since there are seasonal variations in the carotenoid concentration of plants. Moreover, other factors such as age- and sex-specific intake data and considerations of baseline micronutrient status, body mass index, and dietary patterns should be considered for developing more precise KDRI. In particular, the Vit A requirement needs to be met by consuming diverse foods, including animal foods.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.5
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• pp.715-722
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• 1999

On a urea-molasses-straw (3:15:82, UMS) based diet, effect of graded levels of mustard oil cake (MOC) supplementation on the performances of native bulls has been studied. Four cannulated adult Bos indicus bulls of $415({\pm}44.6)kg$ live weight and 80 months old, were given daily either of 0, 200, 400 or 800 g of MOC in four periods in a $4{\times}4$ latin square design. Besides, each animal also received 200 g of each of molasses and wheat bran and a mineral mixture. For unit (1 g) increase in MOC intake, total DM intake increased by 0.8 g/d ($r^2=0.88$) but no change in the straw DM intake. With the increasing levels of MOC, crude protein (CP) digestibility increased exponentially with an asymptotic value of 72%. However, MOC level had no effect on the digestibilities of DM, OM and ADF. Similarly, rumen degradability of rice straw was also not affected by the level of dietary MOC, and mean straw DM degradabilities were 15, 21. 28, 37, 47 and 51% at 8, 16, 24, 48, 72 and 96 hours of incubation respectively. Microbial N yield per kg digestible organic matter apparently fermented in the rumen were 7.46, 8.77, 6.88 and 5.96 g respectively for 0, 200, 400 or 800 g of dietary MOC. For each gram increase in dietary MOC, N intake and N balance increased by 0.054 g/d ($r^2=0.998$) and $0.59mg\;N/kg\;W^{0.75}/d$ ($r^2=0.99$) respectively. Nitrogen balance was estimated to be attained at the N intakes of $246mg\;N/kg\;W^{0.75}/d$. Thus, on a UMS-based diet supplementation of MOC up to 800 g (10% of total intake) of the dietary intake had little or no effect on intake, digestibility, rumen parameters, and microbial N yield but slightly increased the N balance. However marginal response to MOC supplementation is probably due to the high degradability of MOC protein in the rumen. Thus, any substantial positive response of MOC supplementation on a UMS-based diet can probably be achieved by reducing its protein degradability in the rumen.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.187-193
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• 2014

As a result of the cost of grains, the replacement of grains by co-products (i.e. DDGS) in feedlot diets is a common practice. This change produces diets that contain a lower amount of starch and greater amount of fibre. Hypothetically, combining feed grade urea (U) with slow release urea (Optigen) in this type of diet should elicit a better synchrony between starch (high-rate of digestion) and fibre (low-rate of digestion) promoting a better microbial protein synthesis and ruminal digestion with increasing the digestible energy of the diet. Four cannulated Holstein steers ($213{\pm}4$ kg) were used in a $4{\times}4$ Latin square design to examine the combination of Optigen and U in a finishing diet containing different starch:acid detergent fibre ratios (S:F) on the characteristics of digestive function. Three S:F ratios (3.0, 4.5, and 6.0) were tested using a combination of U (0.80%) and Optigen (1.0%). Additionally, a treatment of 4.5 S:F ratio with urea (0.80% in ration) as the sole source of non-protein nitrogen was used to compare the effect of urea combination at same S:F ratio. The S:F ratio of the diet was manipulated by replacing the corn grain by dried distillers grain with solubles and roughage. Urea combination did not affect ruminal pH. The S:F ratio did not affect ruminal pH at 0 and 2 h post-feeding but, at 4 and 6 h, the ruminal pH decreased as the S:F ratio increased (linear, p<0.05). Ruminal digestion of OM, starch and feed N were not affected by urea combination or S:F ratio. The urea combination did not affect ADF ruminal digestion. ADF ruminal digestion decreased linearly (p = 0.02) as the S:F ratio increased. Compared to the urea treatment (p<0.05) and within the urea combination treatment (quadratic, p<0.01), the flow of microbial nitrogen (MN) to the small intestine and ruminal microbial efficiency were greater for the urea combination at a S:F ratio of 4.5. Irrespective of the S:F ratio, the urea combination improved (2.8%, p = 0.02) postruminal N digestion. As S:F ratio increased, OM digestion increased, but ADF total tract digestion decreased. The combination of urea at 4.5 S:F improved (2%, p = 0.04) the digestible energy (DE) more than expected. Combining urea and Optigen resulted in positive effects on the MN flow and DE of the diet, but apparently these advantages are observed only when there is a certain proportion of starch:ADF in the diet.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.29 no.2
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• pp.113-122
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• 2007

Purpose: Platelet-rich plasma (PRP) is known to accelerate and/or enhance hard and soft tissue healing and regeneration. As such, PRP has been used in various clinical fields of surgery. Recently there have been several attempts to use PRP in the field of tissue engineering. However, some controversies still exist on exact mechanism and benefits of PRP. Therefore various animal experiments are necessary to reveal the effect of the PRP. However, even if animal experiment is performed, the efficacy of the experiment could not be validated due to absence of an animal PRP model. The purpose of this study is to establish rat PRP model by comparing several PRP fabricating methods, and to assay growth factor concentration in the PRP. Materials and methods: Rat blood samples were collected from nine SD rat (body weight: 600-800g). PRP was prepared using three different PRP fabricating methods according to previously reported literatures. (Method 1: 800 rpm, 15 minute, single centrifuge; Method 2: 1000 rpm, 10 minute, double centrifuge; Method 3: 3000 rpm, 4min and 2500 rpm, 8 min, double centrifuge). Platelet counts were evaluated in an automated machine before and after PRP fabrications. In terms of growth factor assay, prepared PRP were activated with 100 unit thrombin and 10% calcium chloride. Growth factor (PDGF-BB, VEGF) concentrations on incubation time were determined by sandwich-ELISA technique. Results: An average of 3ml (via infraorbital venous plexus) to 15ml (via celiac axis) the rat blood could be collected. By using Method 3 (3000 rpm, 4 min and 2500 rpm, 8 min, double centrifugation), around 1.5ml of PRP could be prepared. This method allowed us to concentrate platelet 3.77-fold on average. PDGF-BB concentration (mean, 1942.10 pg/ml after 1 hour incubation) and VEGF concentration (mean, 952.71 pg/ml after 1 hour incubation) in activated PRP were higher than those in untreated blood. Also PDGF-BB showed constant concentration during 4-hour incubation, while VEGF concentration was decreased after 1 hour. Conclusion: Total 11,000 g minute separation and condensation double centrifuge method can produce efficient platelet-rich plasma. Platelet-rich plasma activated with thrombin has showed higher concentrations of growth factors such as PDGF-BB and VEGF, compared to the control group. Platelet-rich plasma model in a rat model was confirmed in this study.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.303-311
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• 2020

The acreage of tangerines in the U.S. sharply rose from 19,000 ha in 2009 to 27,000 ha in 2016, an increase of 42% in 7 years. Considering the recent surge in tangerine exports to Japan, the export volume of 6-7 thousand tons is highly likely to increase in the future. Therefore, the purpose of this study was to analyze the economic impact of U.S. tangerine imports on the Korean citrus industry under various scenarios. In order to examine the possibility of imports of U.S. tangerines, the unit price of U.S. exports to Japan was used since U.S. tangerines are not imported to South Korea. Citrus fruits are divided into field citrus, house citrus, and late-maturing citrus (including winter season citrus) based on the cultivation method and variety used to analyze. Considering both the field and house seasons, the import volume of U.S. tangerines can be expected to rise from roughly 4,700 tons in 2021 to 10,000 tons in 2027. Imports of U.S. tangerines may be pushed up or delayed depending not only on the harvest method and quality of domestic field and house citrus but also on the harvest of U.S. tangerines. However, it is necessary to note that tangerines could be imported after 2021, when the tariff rate on U.S. tangerines will fall below 50%.

• Journal of Animal Science and Technology
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• v.59 no.10
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• pp.23.1-23.11
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• 2017

Background: Although soybean meal (SBM) is excellent source of protein in diets for poultry, it is sometimes inaccessible, costly and fluctuates in supply. The SBM can partially be replaced by full-fat SBM, but the meals prepared from raw full-fat soybean contain antinutritional factors. To avoid the risk of antinutritional factors, heat treatment is always advisable, but either excessive or under heating the soybean could negatively affect the quality. However, the potential for further improvement of SBM by supplementing with microbial enzymes has been suggested by many researchers. The objective of this study was to evaluate the performance and ileal nutrient digestibility of birds fed on diets containing raw soybeans and supplemented with microbial protease. Methods: A $3{\times}2$ factorial, involving 3 levels of raw full-fat soybean (RFFS; 0, 45 or 75 g/kg of diet) and 2 levels of protease (0 or 15,000 PROT/kg) was used. The birds were raised in a climate-controlled room. A nitrogen-free diet was also offered to a reference group from day 19 to 24 to determine protein and amino acid flow at the terminal ileum and calculate the standardized ileal digestibility of nutrients. On days 10, 24 and 35, body weight and feed leftover were recorded to calculate the body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). On day 24, samples of ileal digesta were collected at least from two birds per replicate. Results: When RFFS was increased from 0 to 75 g/kg of diet, the content of trypsin inhibitors was increased from 1747 to 10,193 trypsin inhibitors unit (TIU)/g of diets, and feed consumption of birds was also reduced (P < 0.05). Increasing RFFS level reduced the BWG from hatch 0 to 10 d (P < 0.01) and hatch to 24 d (P < 0.05). The BWG of birds from hatch to 35 was not significantly (P = 0.07) affected. Feed intake was also reduced (P < 0.05) during 0 to 35 d. However, protease supplementation improved (P < 0.05) the BWG and FCR during 0 to 24 d. Rising levels of RFFS increased the weight of pancreas (P < 0.001) and small intestine (P < 0.001) at day 24. Except for methionine, apparent and the corresponding standardized ileal digestibility of CP and AA were reduced (P < 0.01) by increasing levels of RFFS in diets. Conclusion: This study showed that some commercial SBM could be replaced by RFFS in broiler diets, without markedly compromising productivity. The AID and SID of CP and lysine were slightly improved by dietary supplementation of microbial protease.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.811-815
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• 2006

The objective of this study was to evaluate the effect of electric fan installation for milk production improvement of dairy cattle in Thailand. The study was conducted using 2 small-holder dairy farms in Chiang Mai province, during April to August 2004. Electric fans were installed in front of each row of cows. Each of the two rows of cows in the barn was defined as an experimental unit, thus each farm had two experimental units. The fans were operated alternately in 7-day intervals between rows of cows within each farm during the day or between 8.00 am to 8.00 pm. Non-operation periods were used as control. Milk yields were recorded. Data on environmental temperature and humidity were obtained from Chiang Mai Meteorological Center. Result from statistically analysis of milk record suggested an interaction between lactation period and fan installation. Therefore, this interaction term of lactation period and fan installation (PERIOD_FAN) was added as a variable to the regression model. Due to the repeated data collection of milk yield from the same cow (alternate week), milk yield was analyzed by repeated measure analysis (Mixed model). Least square means were calculated for all levels and used to compare between each pair-wise values. The final data were collected from the total of 18 cows with 2,072 data. Overall means and SEM of milk yields and days in milk separated into farm were $14.7{\pm}0.06kg/day$ and $176.3{\pm}2.2days$, and $15.2{\pm}0.22kg/day$ and $202.5{\pm}3.7$ days for farm A and farm B, respectively. For multivariable analysis, only PERIOD_FAN and humidity were significantly associated with milk yield. Only the first period of lactation showed that the amount of milk yields during fan installation was higher than that of non-fan installation (p<0.05). Cows with fan installation produced approximately 1.2 kg/cow more milk than cows without fan installation during this period. In conclusion, the use of electric fan operated during the day time increased milk production of cows during the first period of lactation.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.4
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• pp.517-524
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• 2011

The effect on sow milk of variable levels of cassava in lactating sow diets was analyzed in an attempt to explain the beneficial effects reported by producers of including cassava as a basal feed. Twenty crossbred lactating sows were randomly assigned to five dietary treatments. The treatments were: i) broken rice (BR) as the basal feed (BR100), ii) 50% of BR replaced with cassava chip meal (CCM) (CM50), iii) 75% of BR replaced with CCM (CM75), iv) CCM as the basal feed (CM100), and v) dried boiled cassava chips (CCB) as the basal feed (CB100). The hydrocyanide (HCN) content of CCB was reduced to be intermediate between HCN in the no cassava (BR100) and the 50% cassava (CB50) diets. Hydrocyanide was 0.54, 3.24, 4.41, 5.43 and 1.77 ppm in the BR100, CM50, CM75, CM100 and CB100 diets, respectively. Increasing cassava did not affect feed intake (p>0.05), but increased HCN intake (p<0.01). Milk composition was analyzed for protein, fat, lactose, solids not fat (SNF) and total solids (TS). Milk quality was analyzed for total microbes, coliform bacteria, thiocyanate ($SCN^-$), lactoperoxidase (LPO), and glutathione peroxidase (GPx) activity. At farrowing, sow milk composition was not affected by experimental diets (p>0.05), but milk $SCN^-$ increased as the intake of HCN increased in sows diets (p<0.01), $r^2$ = 0.96. At mid-lactation (day 14), milk composition was not affected (p>0.05). The milk quality levels of $SCN^-$ were 9.4, 10.3, 10.5, 11.6 and 9.1 ppm for the BR100, CM50, CM75, CM100 and CB100 diets, respectively (p = 0.01). The LPO contents were 16.41, 42.13, 51.42, 53.94 and 22.81 unit/L, respectively (p = 0.03). There was no GPx activity found in sow milk. When BR was replaced with cassava meal, total microbes and coliforms were reduced 78% and 87%, respectively, by the influences of HCN. The reported beneficial effects of cassava chip meal as a basal feed in lactating sow diets is manifested by improved performance of suckling pigs. This is due to beneficial, non-toxic levels of HCN in the diets. Besides passing HCN to suckling pigs in the form of $SCN^-$, sow milk may also benefit suckling pigs with the observed (day 14) increase in lactoperoxidase content and reduction in coliform bacteria.