• 한국토양비료학회지
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• 제49권3호
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• pp.227-234
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• 2016

The influence of mixing on biogas production and organic material removal performance of solid state anaerobic digestion of dairy manure and sawdust bedding mixtures was evaluated using 22 L volume lab-scale digesters. After 45 days of anaerobic digestion at $37^{\circ}C$, cumulative methane yield of unmixed test unit ($73.1N{\cdot}mL/g-VS$) was almost 1.3 times of that of mixed one ($56.3N{\cdot}mL/g-VS$). The biodegradable volatile solids removal rate of unmixed test unit was 67%, which was almost 28% greater than mixed one. Our results reveal that unmixed condition is better than mixed one in terms of biogas production and organic material reduction.

• Asian-Australasian Journal of Animal Sciences
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• 제15권6호
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• pp.884-889
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• 2002

This paper was carried out from 15 June to 24 December in 2000 in Lake İznik of Turkey, to determine catch per unit effort (CPUE) and size composition of crayfish, Astacus leptodactylus Eschscholtz 1823, captured by fyke-nets of local fishermen. The average CPUE of all size groups was found as 1.65 crayfish/fyke-net/night for fishing season in 2000, but 26.4% of which was below the legal minimum size (crayfish<90 mm). The average CPUE of legal-sized (crayfish$\geq$90 mm) crayfish was 1.10 crayfish/fykenet/night. The annual catch of crayfish was estimated as 2990 tonnes. The length and weight compositions of crayfish captured in the fyke-nets have varied between 60 and 130 mm, and 6 and 71 g, respectively. The mean length and weight were found as 95.5 mm and 28.8 g for all size groups and 100.1 mm and 33.0 g for legal-sized individuals.

• 대한임베디드공학회논문지
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• 제4권3호
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• pp.112-117
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• 2009

An animal assisted therapy (AAT) for handicapped child's psychotherapy has been reported by some researches. A robot can be substituted for the role of a real animal of the AAT. The robot for the AAT is called RAT (Robot Assisted Therapy). It consists of four parts: microprocessor-based MCU(Micro Control Unit), sensing part with various sensors, the movement part operated by some motors, and the exterior with soft feel. We will here introduce the RAT.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.388-394
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• 1999

Two experiments were conducted to subdivide isoleucine (exp. 1) and valine (exp. 2) requirements for maintenance from the requirements for growth of broilers aged 1 to 3 weeks. Purified diets were used, containing five graded levels of isoleucine and valine. Based on weight gain response, the isoleucine requirement for growth was 7.50 mg/g weight gain and the daily isoleucine need for maintenance (mg) was 0.044 per unit metabolic body size ($(Wg^{0.75})$). Based on the N gain response, the isoleucine requirement for growth was 0.317 mg/mg N gain and the daily isoleucine need for maintenance (mg) was 0.040 per unit metabolic body size $(Wg^{0.75})$. Based on weight gain and N gain response, the total isoleucine requirement was calculated 244 mg/day or 0.59% of the diet, 274 mg/day or 0.66% of the diet, respectively. From the relationship of weight gain and N gain, 5.07% of the retained protein was comprised of isoleucine; the reported isoleucine content of chick muscle was 4.42%. The valine requirement for growth was 9.84 mg/g weight gain and 0.36 mg/mg N gain whereas the maintenance requirement was 0.046 or 0.052 mg per unit of metabolic body size (Wgo.11. According to the model developed to estimate valine requirement, the total requirement was 319 mg/day or 0.77% of the diet, 315 mg/day or 0.76% of the diet, respectively. Previous reported valine requirements for growing chicks of 7~24 days old were in close agreement with these estimates. As a percentage of retained protein, valine was calculated to be 5.81% ; the reported valine concentration of crude protein of chicks' body including feathers was 6.72%.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1172-1185
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• 2019

Objective: Meat quality attributes in postmortem muscle tissues depend on skeletal muscle metabolites. The objective of this study was to determine the key metabolic compounds and pathways that are associated with postmortem aging and beef quality in Japanese Black cattle (JB; a Japanese Wagyu breed with highly marbled beef). Methods: Lean portions of Longissimus thoracis (LT: loin) muscle in 3 JB steers were collected at 0, 1, and 14 days after slaughter. The metabolomic profiles of the samples were analyzed by capillary electrophoresis time-of-flight mass spectrometry, followed by statistical and multivariate analyses with bioinformatics resources. Results: Among the total 171 annotated compounds, the contents of gluconic acid, gluconolactone, spermidine, and the nutritionally vital substances (choline, thiamine, and nicotinamide) were elevated through the course of postmortem aging. The contents of glycolytic compounds increased along with the generation of lactic acid as the beef aging progressed. Moreover, the contents of several dipeptides and 16 amino acids, including glutamate and aromatic and branched-chain amino acids, were elevated over time, suggesting postmortem protein degradation in the muscle. Adenosine triphosphate degradation also progressed, resulting in the generation of inosine, xanthine, and hypoxanthine via the temporal increase in inosine 5'-monophosphate. Cysteine-glutathione disulfide, thiamine, and choline increased over time during the postmortem muscle aging. In the Kyoto encyclopedia of genes and genomes database, a bioinformatics resource, the postmortem metabolomic changes in LT muscle were characterized as pathways mainly related to protein digestion, glycolysis, citric acid cycle, pyruvate metabolism, pentose phosphate metabolism, nicotinamide metabolism, glycerophospholipid metabolism, purine metabolism, and glutathione metabolism. Conclusion: The compounds accumulating in aged beef were shown to be nutritionally vital substances and flavor components, as well as potential useful biomarkers of aging. The present metabolomic data during postmortem aging contribute to further understanding of the beef quality of JB and other breeds.

• 한국농공학회논문집
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• 제63권6호
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• pp.117-129
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• 2021

The movement of animals is one of the primary factors that influence the variation of livestock emissions. This study evaluated the relationship between animal activity and three major emissions, PM₁₀, PM_2.5, and ammonia gas, in weaning, growing, and fattening pig houses through continuous monitoring of the animal activity. The movement score of animals was quantified by the developed image analysis algorithm using 10-second video clips taken in the pig houses. The calculated movement scores were validated by comparison with six activity levels graded by an expert group. A comparison between PMs measurement and the movement scores demonstrated that an increase of the PMs concentrations was obviously followed by increased movement scores, for example, when feeding started. The PM₁₀ concentrations were more affected by the animal activity compared to the PM_2.5 concentrations, which were related to the inflow of external PM_2.5 due to ventilation. The PM₁₀ concentrations in the fattening house were 1.3 times higher than those in the weaning house because of the size of pigs while weaning pigs were more active and moved frequently compared to fattening pigs showing 2.45 times higher movement scores. The results also indicated that indoor ammonia concentration was not significantly influenced by animal activity. This study is significant in the sense that it could provide realistic emission factors of pig farms considering animal's daily activity levels if further monitoring is carried out continuously.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.811-815
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• 2001

The study evaluated different synthetic and semisynthetic media for maximal recovery of rumen bacteria and expression of their antibacterial activity. Rumen Glucose Cellobiose Agar (RGCA) medium was found to be the best for recovery of rumen bacteria. However, L-10 medium was the best for expression of antibacterial activity of ruminal isolates followed by Easy, M-10, RGCA and M-98-5 medium. The present study recommends the use of L-10 medium as the medium of choice for screening of antibacterial activity of ruminal isolates. Comparative evaluation of bacterial counts on different dietary regimes indicated significant difference between different growth media on a specific diet and between diets on specific growth media within a species. However, there is no overall significant difference between total bacterial counts obtained from rumen liquor of cattle and buffalo with respect to either the feeding regime or growth media. Feeding straw based diet to the animal is the best for high recovery of rumen bacteria.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1355-1360
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• 2008

Effects of dietary ${\delta}$-aminolevulinic acid (ALA) supplementation on serum iron status, blood characteristics, egg production and quality were examined in laying hens in an 8-week feeding trail. Two hundred and forty (Hy-line brown, 40-week-old) layers were randomly assigned to four dietary treatments with ten replications (six layers in adjacent three cages). Dietary treatments included: 1) CON (basal diet), 2) ALA1 (CON+ALA 5 ppm), 3) ALA2 (CON+ALA 10 ppm) and 4) ALA3 (CON+ALA 15 ppm). All nutrient levels of diets were formulated to meet or exceed NRC (1994) recommendations for laying hens. During the entire experimental period, differences of serum iron concentration and total iron binding capacity (TIBC) were significantly increased in ALA1 supplemented treatment (quadratic effect, p<0.05). The difference of total protein between 8 and 0 weeks was significantly higher in ALA2 treatment than CON treatment (quadratic effect, p<0.05). No significant effects were observed on hemoglobin, WBC, RBC, lymphocyte and albumin concentrations. Egg production and egg weight were not influenced by the ALA supplementation. Egg yolk index was also significantly higher in ALA3 treatment than CON treatment at the end of 4 and 8 weeks (linear effect, p<0.05). Haugh unit was increased in ALA3 treatment compared to CON and ALA1 treatments at the end of 8 weeks (linear effect, p<0.05). However, egg shell thickness, breaking strength and yolk color unit were not affected by the ALA supplementation. In conclusion, dietary ALA supplementation at a level of 5 ppm can affect iron concentration in serum while higher levels (10 or 15 ppm) have some beneficial influences on blood profiles and egg quality.

• Asian-Australasian Journal of Animal Sciences
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• 제14권5호
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• pp.655-667
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• 2001

This experiment was conducted to investigate the effect of feeding a low CP diet supplemented with synthetic amino acids on performance, nutrient utilization and carcass characteristics of finishing pigs fed under a three-phase feeding regimen. Ninety-six finishing pigs (Landrace$\times$Large White$\times$Duroc), $55.75kg{\pm}0.65$ of initial body weight, were blocked by weight and sex and allotted to four dietary treatments in a randomized block design. There were six pens per treatment and four pigs per pen. Pigs were fed a 16%-14%-12% CP (for phase I-II-III, respectively), sequence of diets. Dietary treatments were 1) Control, 2) Con+L (a sequence of diets reduced in CP by l percentage unit with lysine (L) supplementation, 3) Con+LMT (a sequence of diets reduced in CP by 2 percentage unit with LYS, methionine (MET) and threonine (THE) supplementation) and 4) Con+LMTT (a sequence of diets reduced in CP by 3 percentage unit with LYS, MET, THR and tryptophan (TRP) supplementation). The finishing period (55 to 105 kg) was divided into three phases (55 to 72 kg, 72 to 90 kg and 90 to 105 kg). Pigs fed either the control or Con+L diet grew faster (p<0.05) than pigs fed the Con-LMT or Con+LMTT diet. There was no difference in ADFI among dietary treatments. Phosphorus (P) digestibility was lowest in the control group and highest in the Con+LMTT group (p<0.05). Within each phase, no significant differences in dry matter (DM) and CP digestibilities were found. Although some amino acid digestibilities were affected by dietary treatments, digestibilities of essential amino acids (EAA), non-essential amino acids (NEAA) and total amino acid were not significantly influenced by dietary treatments. For the entire experiment periods, Con+L, Con+LMT and Con+LMTT treatments resulted in 13.4, 18.8 and 21.6% lower total N excretion compared with the control. Con+LMT and Con+LMTT treatments showed significantly lower BUN concentration compared with the control and Con+L treatment (p<0.05), but there was no significant difference in BUN concentration between pigs fed the control and Con+L treatment or between pigs fed Con+LMT and Con+LMTT treatments (p>0.05). Carcass length, backfat thickness and carcass grade were not significantly affected by dietary treatments (p>0.05). In conclusion, reducing dietary CP level by 1 percentage unit and supplementing only LYS at each phase could be a very beneficial feeding strategy for finishing pigs fed under a three phase feeding regimen in terms of both environmental and economical aspects.

• 한국초지조사료학회지
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• 제29권4호
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• pp.383-388
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• 2009

본 연구는 혼파초지에서 유 산양을 방목 이용하였을 때의 건물섭취량, 증체량 및 두당 방목초지의 소요면적을 산출하고자 2007년 4월부터 2008년 6월까지 충남대학교 동물사육장내 혼파초지에서 수행하였다. 공시초지는 2006년 9월 21일 orchardgrass (40%) + tall fescue (20%) + perennial ryegrass (10%) + alfalfa (15%) + red clover (15%)를 ha당 30 kg 파종하여 경운초지로 조성한 혼파초지에서 수행하였다. 방목면적은 총 $5,000\;m^2$로 4목구(구당 평균 $1,250\;m^2$)의 윤환 방목으로 12회 방목하였다. 공시축은 Saanen종으로 13두(평균 31.1 kg)를 방목하였다. 방목기간 중 월 별로 조사한 혼파초지의 평균 조단백질 함량은 20.4%, NDF와 ADF 함량은 각각 65.3%와 31.1%이었으며, 건물소화율은 68.9%이었다. 두당 평균 건물섭취량은 1.253 kg 이었으며, 체중 당 3.01%을 섭취하였다. 방목기간 (184일)의 두당 평균 증체량은 17.4 kg이었고, 일당증체량은 98g이었다. 유 산양(체중 50 kg 기준)의 두당 방목초지의 소요면적은 $467.7\;m^2$로 산출되었는데 이를 Animal unit (AU/ha)로 환산하면 약 2.14 AU/ha에 해당하였다.