• Title/Summary/Keyword: Animal research

Search Result 10,574, Processing Time 0.045 seconds

Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm

  • Ching-Fu Tu;Shu-Hui Peng;Chin-kai Chuang;Chi-Hong Wong;Tien-Shuh Yang
    • Animal Bioscience
    • /
    • v.36 no.2_spc
    • /
    • pp.339-349
    • /
    • 2023
  • Gene editing (GE) offers a new breeding technique (NBT) of sustainable value to animal agriculture. There are 3 GE working sites covering 5 feasible pathways to generate GE pigs along with the crucial intervals of GE/genotyping, microinjection/electroporation, induced pluripotent stem cells, somatic cell nuclear transfer, cryopreservation, and nonsurgical embryo transfer. The extension of NBT in the new era of pig breeding depends on the synergistic effect of GE and reproductive biotechnologies; the outcome relies not only on scientific due diligence and operational excellence but also on the feasibility of application on farms to improve sustainability.

Vitrification of Bovine Immature Oocytes using Microdrop Method

  • Park, H.S.;Kim, D.H.;Kim, S.W.;Yang, B.C.;Im, G.S.;Hwang, I.S.;Seo, J.S.;Yang, B.S.;Moon, S.J.;Chang, W.K.
    • Proceedings of the KSAR Conference
    • /
    • 2004.06a
    • /
    • pp.258-258
    • /
    • 2004
  • Successful cryopreservation of mammalian oocytes would provide a source of materials for in vitro embryo production. This study was conducted to determine vitrification conditions for bovine immature oocytes using micro-drop method and, to examine maturation, fertilization and development of vitrified bovine immature oocytes. (omitted)

  • PDF

The Differential Gene Expression Patterns in Endometrial Tissue of Sows in Early Gestation

  • Kim, Bong-Ki;Chung, Hak-Jae;Ko, Yeoung-Gyu;Lee, Hwa-Young;Woo, Jei-Hyun;Kim, Young-Mi;Bae, Young-Eun;Kim, Jeom-Soon;Jung, Jin-Kwan;Chang, Won-Kyong
    • Proceedings of the KSAR Conference
    • /
    • 2004.06a
    • /
    • pp.235-235
    • /
    • 2004
  • Although studies for important genes concerned in preimplantation stage that encompasses the period from fertilization to implantation were reported for mice and cows, little information relevant to this subject is known in pigs. (omitted)

  • PDF

Porcine ear necrosis syndrome by coinfection of porcine reproductive and respiratory syndrome virus and Staphylococcus hyicus (PRRSV와 Staphylococcus hyicus 복합감염에 의한 돼지 귀 괴사 증후군 증례 보고)

  • Lee, Seunghee;Jung, Ji-Youl;Kim, Seong-Hee;Kim, Jong-Wan;Park, Jung-Won;Kang, Dae-Young;Her, Ji-Woong;Jeong, Yea-Ji;So, Byung Jae;Yoon, Soon-Seek
    • Korean Journal of Veterinary Research
    • /
    • v.57 no.2
    • /
    • pp.143-146
    • /
    • 2017
  • Porcine ear necrosis syndrome is characterized by erosive and ulcerative lesions at the margin or tip of the pinna. Three growing pigs of different ages exhibited retarded growth accompanied by reddening and necrosis of ear prior to death. Gross examination showed reddening, swelling, black discoloration, scaling, and variable-sized yellowish materials and edema in ear cross section. Microscopically, thrombosis, abscess, ulceration, epidermal hyperplasia, and dermal pyogranulomatous inflammation with an intralesional bacterial colony were observed. Staphylococcus hyicus was isolated in all pigs' ears and porcine reproductive and respiratory syndrome virus was detected by PCR and immunohistochemistry.