• 한국수산과학회지
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• 제47권6호
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• pp.824-831
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• 2014

This study was conducted to investigate the effects of fishmeal replacement with acid-concentrated soybean meal (ACSBM) on growth performance, blood biochemistry, and ingredient digestibility in juvenile olive flounder Paralichthys olivaceus. Six experimental diets were formulated to replace fishmeal protein with ACSBM at 0%, 20%, 30%, 40%, 50%, and 60% (designated ACSBM0, ACSBM20, ACSBM30, ACSBM40, ACSBM50, and ACSBM60, respectively). Triplicate groups of fish (initial fish mean weight: $14.3{\pm}0.03g$) were fed the experimental diets to apparent satiation (twice daily at 08:00 and 18:00 h). After a 12-week feeding trial, a total of 180 healthy fish were randomly distributed into three Guelph system tanks at a density of 60 fish/tank (initial fish mean weight : $50.6{\pm}2.4g$) to test the apparent digestibility coefficients of the ingredients (ACSBM, fishmeal, and soybean meal). Although negative effects were observed with ACSBM40, ACSBM50 and ACSBM60 after 12 weeks of feeding, up to 20% of the fishmeal protein could be successfully replaced with ACSBM without significant growth depression. Hemoglobin and hematocrit values of fish fed the ACSBM50 and ACSBM60 diets were significantly lower than those of fish fed the ACSBM0 diet. Glucose values of fish fed the ACSBM60 diet were significantly higher than those of fish fed the ACSBM0 and ACSBM20 diets. Digestibility of protein in ACSBM and soybean meal was 85.9% and 82.5%, respectively. Results indicated that at least 20% of fishmeal protein can be replaced by ACSBM in diets of juvenile olive flounder without supplementation of limiting amino acids.

• 대한기계학회논문집B
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• 제35권11호
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• pp.1205-1211
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• 2011

본 논문은 말기 심질환 환자의 생존율과 치료효과를 증대시키기 위한 심실보조장치용 심박조율기의 개발에 대한 내용이다. 심실보조장치만을 이식 받은 환자는 부정맥이 발생할 가능성을 항상 갖고 있으며 심박조율기를 동시에 적용함으로써 혈류의 체순환량을 유지하고 부정맥으로 인한 장기의 손상을 예방한다. 심박조율기의 전극과 도선은 심실보조장치의 도관을 이용하여 제작되었으며, 전극은 심실보조장치의 도관이 삽관되는 좌심실의 심첨부에 도관과 함께 이식된다. 심박조율기는 0 bpm에서부터 191.4 bpm까지 자극 빈도를 조절할 수 있으며, 60 bpm의 빈도로 자극이 가하여 질 때 0.25 J 의 에너지가 심장으로 인가된다. 심실보조장치용 심박조율기의 성능과 안정성을 검증하기 위하여 돼지를 이용한 동물실험을 수행하였다. 실험동물의 심장에 86.4 bpm, 100.2 bpm, 126.6 bpm의 자극을 순차적으로 가하였고 ECG 및 대동맥혈압의 변화 대동맥 혈류를 관찰하였다.

• 한국토양비료학회지
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• 제41권5호
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• pp.318-323
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• 2008

간척성토지에서 SCBLF 처리효과를 알아보기 위하여 80일 동안 SCBLF 처리 후 포플러의 클론별 생육특성을 조사하였다. 엽면적은 SCBLF 처리구가 대조구에 비해 26% 증가하였다(P<0.05). 엽록소 함량은 처리구가 대조구보다 5% 증가하였으나 유의성은 없었다. 엽면적의 경우 Suwon, Clivus, 72-30, Dorskamp, 97-19서 처리구와 대조구간 통계적 차이가 있었고 (P<0.05), 엽록소 함량은 97-19에서 유의성을 나타났다(P<0.01). 잎의 T-N함량은 P<0.01에서 유의성이 인정되었으며, 클론별로는 97-19, I-476, Suwon, 72-30이 P<0.05, Clivus가 P<0.01 수준에서 유의성을 보였다. 수고 및 흉고직경 생장은 SCBLF 처리구가 대조구에 비해 각각 20%, 41% 증가하였으며 유의성이 인정되었다(P<0.01). 수고생장은 Clivus, 72-30, Suwon에서(P<0.05) 흉고직경생장은 Clivus, Eco28, Dorskamp,72-30, 72-31에서 유의성이 인정되었다(P<0.01). 특히 현사시(Clivus, 72-30)의 경우 잎의 엽면적, T-N함량, 수고 및 흉고직경 생장에서 통계적으로 유의한 차이를 보였으며 SCBLF 처리구가 대조구보다 높거나 우수하였다.

• 생명과학회지
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• 제17권10호
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• pp.1321-1329
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• 2007

사슴뿔은 동물세계에서 가장 빨리 성장하는 조직이다. 따라서 성장중인 사슴뿔은 뼈 성장을 촉진하는 인자가 풍부하게 포함된 것으로 생각된다. 이들 성장인자들 중 IGF-1은 뼈를 자라게 하는 조골세포의 대사에 중요한 역할을 한다고 알려져 있어 이를 정제하고자 하였다. IGF-1의 정제는 상대라고 불리는 신선한 사슴뿔을 유안침전, DEAE-Sepharose CL-6B 이온교환수지, CM-Sepharose CL-6B 양이온교환수지, Sephadex G-50의 순차적인 방법으로 할 수 있었다. 각 과정마다 IGF-1의 거동을 HPLC, SDS-PAGE, Dot blot, 그리고 western blot으로 분석하였다. IGF-1의 정량은 ELISA기술로 재조합 인간 IGF-1을 이용하여 계산되었으며, 최종 분별 액은 두 개의 단백질을 보였으나, Western-blot에서 작은 분자량인 12 kDa으로 최종 판명할 수 있었다. 정제된 단백질은 HPLC에서 retention 시간 8분만에 검출되었으며, 총 농도는 2910 ng/ml 이고 중량은 0.291 g 이었다.

• 대한의용생체공학회:의공학회지
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• 제29권1호
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• pp.32-39
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• 2008

This paper reports on a realtime OS based master-slave configuration robot control system for laparoscopic surgery robot which enables telesurgery and overcomes shortcomings with conventional laparoscopic surgery. Surgery robot system requires control system that can process large volume information such as medical image data and video signal from endoscope in real-time manner, as well as precisely control the robot with high reliability. To meet the complex requirements, the use of high-level real-time OS (Operating System) in surgery robot controller is a must, which is as common as in many of modem robot controllers that adopt real-time OS as a base system software on which specific functional modules are implemened for more reliable and stable system. The control system consists of joint controllers, host controllers, and user interface units. The robot features a compact slave robot with 5 DOF (Degree-Of-Freedom) expanding the workspace of each tool and increasing the number of tools operating simultaneously. Each master, slave and Gill (Graphical User Interface) host runs a dedicated RTOS (Real-time OS), RTLinux-Pro (FSMLabs Inc., U.S.A.) on which functional modules such as motion control, communication, video signal integration and etc, are implemented, and all the hosts are in a gigabit Ethernet network for inter-host communication. Each master and slave controller set has a dedicated CAN (Controller Area Network) channel for control and monitoring signal communication with the joint controllers. Total 4 pairs of the master/slave manipulators as current are controlled by one host controller. The system showed satisfactory performance in both position control precision and master-slave motion synchronization in both bench test and animal experiment, and is now under further development for better safety and control fidelity for clinically applicable prototype.

• 한국산학기술학회논문지
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• 제16권9호
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• pp.6236-6246
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• 2015

논문에서는 무인 발정발현 관찰을 통해 다두 사육 시 발생하는 공태를 줄이고, 정확한 수정적기 판단을 통해 번식률을 향상하고자 하였다. 무리 생활을 하는 소의 특성상 운동량의 증가 유무만으로는 발정 발현의 판단이 어렵기 때문에, 소의 운동량 수집 센서와 개체 정보 종합 관리 프로그램을 개발하여 무인 발정발현 관찰시스템을 구축하고 운동량 정보와 번식 정보를 이용하여 발정 발현 관찰 알고리즘을 개발하였다. 시스템과 알고리즘에 대한 성능 검증은 수정 전 시스템을 통해 수정적기를 관찰하고, 수정하고 21일 후 재발정 여부를 확인, 다음 21일 후 초음파 진단을 통해 수태여부를 확인하여 적절하게 수정적기를 판단하였는지 검증하였고, 이를 규모가 유사한 실제 축산 농가 4곳에서 수행하였다. 각 농가의 총 사육 두수는 87, 81, 93, 82두이며 관찰된 발정 예정인 소는 14, 19, 15, 17두 이다. 이중 미약발정에 해당하는 3, 2, 1, 3두는 관찰에 실패하였으나 정상발정에 해당하는 11, 17, 14, 14두의 발정을 감지하였고, 인공 수정 후 10(91%), 17(100%), 13(93%), 14(100%)두의 소가 수태하여 제안된 무인 발정발현 관찰 시스템이 정상발정 시 수정적기 판단에 유효함을 보였다.

• 한국약용작물학회지
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• 제26권4호
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• pp.271-280
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• 2018

Background: Astragalus membranaceus is a well known oriental medicinal herb. The polysaccharides of the aboveground parts (AMA) and the radix (AMR) of A. membranaceus are the most important functional constituents. Methods and Results: The aim of this study was to determine the effects of AMA and AMR on the oxidative damage induced in the skeletal muscle of rats subjected to exhaustive exercise. Sprague-Dawley rats were randomly divided into exercise and non-exercise groups; in the groups receiving the test compounds, AMA and AMR were administered orally for 30 days. Skeletal muscle samples were collected from each rat after running to exhaustion on a treadmill to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) and the concentation of malondialdehyde (MDA). The antioxidant enzyme activities of SOD and GSH-Px of skeletal muscle of AMA- and AMR-treated groups were significantly higher than those of the control and commercial sports drink (SPD)-treated groups in exhaustive exercise rats. In addition, MDA concentrations in the skeletal muscle of the AMA- and AMR-treated groups were significantly lower than those of the control and SPD-treated groups. In the present study, the effects of AMA and AMR on exercise endurance capacity were also evaluated in mice subjected to a swimming exercise test. AMA and AMR supplementation prolonged the swimming time of mice and enhanced exercise endurance capacity. AMA and AMR possess the ability to retard and lower the production of blood lactate, and prevent the decrease of serum blood glucose. Conclusions: These results showed that, AMR and AMA exerted beneficial effect in mice, increasing the activity of the antioxidant systems and inhibiting oxidative stress induced by exhaustive exercise. The compounds improved exercise performance and showed anti-fatigue effects against exhaustive exercise.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.55-65
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• 2002

Ginseng has been used as a key constituent in traditional medicine prescriptions for centuries. Other than its well-known anti-stress and adaptogenic properties, ginseng has also been shown to be very effective in treating age-related deterioration in metabolic and memory functions. Although it is generally believed that the saponin (GS) fraction of the ginseng root accounts for the bioactivity of ginseng, a direct demonstration on which ginsenoside does what is still generally lacking. In the past decade, our laboratory has endeavored to identify the active GS components involved in energy metabolism, memory, and anti-neurotoxicity. To examine the ergogenic effects of GS in enhancing aerobic capacity, rats were subjected to either severe cold ($40^{\circ}C$ under helium-oxygen, two hours) or exercise workload $(70\%\;VO_{2}max,$ to exhaustion). Acute systemic injection (i.p.) of ginseng GS (5-20 mg/kg) significantly elevated both the total and maximum heat production in rats and improved their cold tolerance. However, pretreating the animal with the optimal dose (10 mg/kg) of GS devoid of $Rg_1\;and\;Rb_1$ failed to elicit any beneficial effects in improving cold tolerance. This indicates that either $Rb_1\;and/or\;Rg_1$ may be essential in exemplifying the thermogenic effect of GS. Further studies showed that only pretreating the animals with $Rb_1(2.5-5\;mg/kg),\;but\;not\;Rg_l,$ resulted in an increase in thermogenesis and cold tolerance. In contrast to the acute effect of GS on cold tolerance, enhancement of exercise performance in rats was only observed after chronic treatment (4 days). Further, we were able to demonstrate that both $Rb_1\;and\;Rg_1$ are effective in enhancing aerobic endurance by exercise. To illustrate the beneficial effects of GS in learning and memory, a passive avoidance paradigm (shock prod) was used. Our results indicated that the scopolamineinduced amnesia can be significantly reversed by chronically treating (4 days) the rats with either $Rb_1\;or\;Rg_1$ (1.25 - 2.5 mg/kg). To further examine its underlying mechanisms, the effects of various GS on ${\beta}-amyloid-modulated$ acetylcholine (ACh) release from the hippocampal slices were examined. It was found that inclusion of $Rb_1$ (0.1 ${\mu}M$), but not $Rg_1$, can attenuate ${\beta}-amyloid-suppressed$ ACh release from the hippocampal slices. Our results demonstrated that $Rb_1\;and\;Rg_1$ are the key components involved in various beneficial effects of GS but they may elicit their effects through different mechanisms.

• 한국건축시공학회지
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• 제19권1호
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• pp.9-18
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• 2019

현대에 가장 널리 쓰이는 건축 재료인 콘크리트는 기술의 지속적인 발전에 따라 고강도화 뿐만 아니라 인성 및 연성의 증가, 경량화와 같은 구조적 성능의 향상이 되었다. 또한 인간의 삶의 질이 향상됨에 따라 감성을 충족시킬 수 있는 것에 대한 수요의 급증으로 건축용 외장패널 그리고 건축의 경계를 넘어 인테리어 소품으로까지 다양하게 쓰이는 추세이다. 국내에서는 플라스틱 봉을 삽입하여 빛과 콘크리트의 결합으로 사용자의 감성을 자극하는 빛 감성친화형콘크리트(LEFC)를 개발하였으나, 높은 단위중량으로 인한 현장에서의 시공성 한계를 보여주었다. 이에 본 연구에서는 LEFC에 기포제를 적용하여 단위중량을 감소시켜 경량화를 달성하고 휨 성능 향상을 위해 두 가지 유기섬유(Nylon Fiber, Polyvinyl Alcohol)를 혼입하여 비교분석하였다. 마지막으로 플라스틱 봉 삽입으로 인한 콘크리트 비표면적 손실 및 봉과의 부착력 감소로 인한 휨 강도 변화를 봉의 직경(5mm, 10mm)과 간격(10mm, 15mm, 20mm)에 따른 변수를 적용한 예측 모델을 제안하고자 한다.

• Korean Journal of Radiology
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• 제19권6호
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• pp.1161-1171
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• 2018

Objective: The aim of this study was to investigate diffusion tensor (DT) imaging-derived properties of benign oligemia, true "at risk" penumbra (TP), and the infarct core (IC) during the first 3 hours of stroke onset. Materials and Methods: The study was approved by the local animal care and use committee. DT imaging data were obtained from 14 rats after permanent middle cerebral artery occlusion (pMCAO) using a 7T magnetic resonance scanner (Bruker) in room air. Relative cerebral blood flow and apparent diffusion coefficient (ADC) maps were generated to define oligemia, TP, IC, and normal tissue (NT) every 30 minutes up to 3 hours. Relative fractional anisotropy (rFA), pure anisotropy (rq), diffusion magnitude (rL), ADC (rADC), axial diffusivity (rAD), and radial diffusivity (rRD) values were derived by comparison with the contralateral normal brain. Results: The mean volume of oligemia was $24.7{\pm}14.1mm^3$, that of TP was $81.3{\pm}62.6mm^3$, and that of IC was $123.0{\pm}85.2mm^3$ at 30 minutes after pMCAO. rFA showed an initial paradoxical 10% increase in IC and TP, and declined afterward. The rq, rL, rADC, rAD, and rRD showed an initial discrepant decrease in IC (from -24% to -36%) as compared with TP (from -7% to -13%). Significant differences (p < 0.05) in metrics, except rFA, were found between tissue subtypes in the first 2.5 hours. The rq demonstrated the best overall performance in discriminating TP from IC (accuracy = 92.6%, area under curve = 0.93) and the optimal cutoff value was -33.90%. The metric values for oligemia and NT remained similar at all time points. Conclusion: Benign oligemia is small and remains microstructurally normal under pMCAO. TP and IC show a distinct evolution of DT-derived properties within the first 3 hours of stroke onset, and are thus potentially useful in predicting the fate of ischemic brain.