• Title/Summary/Keyword: Animal material

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Increase of ICSI Efficacy with Hyaluronic Acid-Binding Sperm for Low Aneuploidy Frequency in Pig

  • Park, Chun-Young;Uhm, Sang-Jun;Song, Sang-Jin;Kim, Kwag-Sung;Hong, Seung-Bum;Chung, Kil-Saeng;Lee, Hoon-Taek
    • Proceedings of the KSAR Conference
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    • 2003.06a
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    • pp.26-26
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    • 2003
  • Hyaluronic acid (HA)-binding sites have been shown the diagnostic potential fur assessment of sperm maturity, which is related to male fertility. This study was designed to evaluate chromosomal patterns in porcine embryos produced by in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) with non- or HA-binding sperm (HABS). For binding of sperm with HA, sperm incubated in 10 ${mu}ell$ drop containing HA (0.8 mg/ml)-agarose (0.8%) mixture for 15 min. IVF and ICSI with non- or HA-bound sperm examined with matured oocytes at 44 hr after in vitro maturation. Embryos were cultured in 50 ${mu}ell$ of NCSU 23 containing 0.5% BSA for 5 days and then in 50 ${mu}ell$ of NCSU 23 containing 10% FBS for 2 days. For the evaluation of chromosomal aneuploidies, chromosome 1 sub-metacentric specific probe was used in sperm and embryos by fluorescence in situ hybridization (FISH). The frequency of aneuploidy sperm for chromosome 1 was 6.25%. The significant differences following IVF and ICSI with non- or HA-bound sperm were not observed in blastocyst formation rates (18.6, 23.5, and 23.8%) and cell number (61.8 $\pm$ 12.5, 55.5 $\pm$ 7.3, and 59.3 $\pm$ 9.6). Moreover, the percentage of diploidy in 4-cell stage embryos was 57.1% (IVF), 68.8% (ICSI), and 76.3% (ICSI-HABS). These results suggest that HA-binding sites may be a material for selection of normal sperm for ICSI. Therefore HA selection of normal sperm may be reduce the loss to embryonic mortality prior to embryo transfer in pig.

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A study on the animal figures in Scytian Ornament -focusing on the single animal figures (스카타이계 장식품에 나타난 동물문에 대한 연구 -단독동물문을 중심으로-)

  • 김문자
    • Journal of the Korean Home Economics Association
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    • v.38 no.8
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    • pp.13-27
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    • 2000
  • The background of single animal figures was originated from those northern mounted nomadic groups, which was Scythe style Culture. The art of the nomads working in the Scythian idiom was small in size and essentially decorative in intention, yet practically every object which can be associated with any unit in this group of people possesses many of the attributes essential to a real work of art. Clarity of conception, purity of form, co-ordination of rhythm and balance, and not least, an understanding and respect for the material employed were triumphantly blended by the Eurasian nomads to produce a distinctive style. In Scythian art the multitude of animal representations well illustrates the reoccupation of this nomadic people with animals in their environment. Usually only wild animals are represented. Commonly depicted are: stags and deer, lions or other large cats, eagles, birds heads (perhaps of ravens), griffins, snakes, hares, fish, goats, rams, boars, moose (elk), yak, sheep and bears. The occasional exception to the wild animal rule is domesticated horses-important because the Scythians were horse bleeders and their whole culture revolved around their dependence on the horse. The nomads had little reason to create object in honour of gods or men, but they had an instinct for beauty and the wish to surround themselves with the animal forms in which they had come to delight The Scytians tried to combine in a single rendering all the salient points of the animal they were delineating. They archived considerable success in the difficult task of showing in a single image the various and often incompatible poses assumed by a single animal in the course of its life. Zoomorphic motifs were used not simple for decorative effect, but to trim the object into amulets, with magical power to assist in hunting, and to protect the owner from harm.

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Effects of Salt Concentration in Soybean Sauce on the Physicochemical Properties of Pre-rigor Ground Hanwoo Muscle

  • Kim, Hyun-Wook;Choi, Ji-Hun;Choi, Yun-Sang;Kim, Hack-Youn;Han, Doo-Jeong;Kim, Tae-Hyun;Lee, Sung-Ki;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.31 no.3
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    • pp.389-397
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    • 2011
  • This study was conducted to examine the effect of salt concentration in soybean sauce (ganjang) on pre-rigor ground Hanwoo (Korean native cattle) muscle. Although adding soybean sauce reduced pH of pre-rigor Hanwoo muscle, it improved the water holding capacity (WHC), cooking loss, total protein solubility, myofibrillar fragmentation index (MFI), 2-thiobar-bituric acid (TBA) values, and textural properties by increasing salt concentration in the soybean sauce. In particular, adding soybean sauce inhibited lipid oxidation despite increasing salt concentration. An increased salt concentration in the soybean sauce in pre-rigor Hanwoo muscle tended to make the meat have lower lightness and higher redness and yellowness values. Although soybean sauce had a significantly lower pre-rigor salting effect than sodium chloride in terms of cooking loss and total protein solubility (p<0.05), soybean sauce improved myofibrillar fragmentation and lipid oxidation when compared with sodium chloride. Furthermore, no significant differences in textural properties were observed between adding soybean sauce and sodium chloride at the same salt concentrations. Therefore, soybean sauce can be a functional curing material for pre-rigor muscle.

Effect of line and floor type on growth performance and feather characterization during the growth period of White Roman geese

  • Lin, Min Jung;Chang, Shen Chang;Chen, Tzu Jou;Lin, Wei Chih;Peng, Shao Yu;Lee, Tzu Tai
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.9
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    • pp.1455-1462
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    • 2020
  • Objective: The purpose of this study was to investigate whether goose growth and feather characteristics are influenced by their line and feeding surroundings, inclusive of floor materials and types, since there are no reports regarding these factors. Methods: The 240 White Roman geese which were hatched and sex identified came from 3 commercial goose farms. They were randomly distributed to 24 pens depending on a completely random design. The study continued for 13 weeks and included 3 lines of commercial geese and 2 floor types (cement strip floor [CSF] or cement floor [CF]). Results: The day one gosling weight from A farm was lower than other two farms (96 g vs 107 and 115 g; p<0.001). Afterwards, the body weight, back length, keel length, chest girth and main wing feather length among 3 farms showed no significance difference prior to 12 weeks. The CF group showed heavier body weight, shorter back length, longer keel length, shorter chest girth and shorter main wing feather length than the CSF group prior to 12 weeks. The down weight in the CF was heavier than the CSF group (57.1 g vs 41.8 g; p<0.01) prior to 13 weeks. Conclusion: The body weight showed the positive relations for dry feather weight (r = 0.59), down weight (r = 0.69), percent of the down weight of live body weight prior to 13 weeks (r = 0.61).

Effects of surface materials of self-draining beds on cattle behavior in a temperate climate

  • Liu, Ping;Guo, Lulu;Zhang, Fulan;Li, Lin;Mao, Huaming;Gu, Zhaobing
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.11
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    • pp.1866-1872
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    • 2020
  • Objective: The objective of the present experiment was to construct self-draining beds to keep surface bedding materials clean and dry for beef cattle comfort in a temperate climate. Methods: In Experiment 1, a self-draining bed was covered with sand at depths of 10 cm (S-10a), 15 cm (S-15), and 20 cm (S-20) respectively. In Experiment 2, self-draining beds of different sizes were covered with 10 cm of sand (S-10b) and wood shavings (WS) at depths of 15 cm and 20 cm (WS-15 and WS-20). Fifteen cattle were engaged to evaluate the comfort of self-draining beds covered with different bedding materials. Results: No cattle lay in the feed alley and cattle spent more time lying on S-10a than S-15 or S-20 in Experiment 1 (p<0.01). No difference in lying time was detected between S-15 and S-20 (p>0.05). In Experiment 2, no cattle selected the feed alley as the lying area. Cattle preferred WS-15 as the lying area and time spent lying on WS-20 was slightly higher than on S-10b (p<0.05). Feces weight was higher in the feed alley than in the different bedding areas in both Experiments 1 and 2 (p<0.01). Conclusion: Sand-bedding depth at 10 cm and WSs at 15 cm above the self-draining bed can provide for the lying comfort of beef cattle. Design of a special feed alley to hold most of the feces to keep bedding materials clean and dry is desirable for organic beef cattle in a loose barn.

An Analysis of Survey Data on South Korea Boar Stud Practices (한국의 돼지 인공수정센터 현황 분석)

  • Sa, Soo-Jin;Woo, Jae-Seok;Hong, Joon-Ki;Kim, Ki-Hyun;Kim, Du-Wan;Kim, Yong-Min;Park, Hyun-Sik;Kim, Si-Joo;Chung, Ki-Hwa;Cho, Eun-Seok
    • Journal of Embryo Transfer
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    • v.30 no.3
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    • pp.129-135
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    • 2015
  • The aim of this study is to examine current status of swine AI and boar stud in South Korea using survey and data analysis. This survey included 48 boar studs registered as 'semen processing business'. The survey data were collected by direct visitation, FAX and/or telephone conversation for 7 months from June through December in 2013. 48 boar studs owned a total of 3,537 boars and the Duroc breed accounted for the highest rate (75.3%) of all boar breeds. In case of ownership, agricultural management corporations was the highest (50.0%) and followed by individual ownership (33.3%). Large-scale boar studs in terms of own over 151 boar were surveyed as 4.2% and most boar studs owned less than 100 boars (77.1%). The amount of liquid semen provided by 48 boar studs were 1,889,000 doses and each boar stud provided average of 39,000 does, which is represented for 90% consumption by sows in South Korea.

Effects of Horse Meat Hydrolysate on Oxidative Stress, Proinflammatory Cytokines, and the Ubiquitin-Proteasomal System of C2C12 Cells

  • Hee-Jeong Lee;Dongwook Kim;Kyoungtag Do;Chang-Beom Yang;Seong-Won Jeon;Aera Jang
    • Food Science of Animal Resources
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    • v.44 no.1
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    • pp.132-145
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    • 2024
  • Sarcopenia, the age-related muscle atrophy, is a serious concern as it is associated with frailty, reduced physical functions, and increased mortality risk. Protein supplementation is essential for preserving muscle mass, and horse meat can be an excellent source of proteins. Since sarcopenia occurs under conditions of oxidative stress, this study aimed to investigate the potential anti-muscle atrophy effect of horse meat hydrolysate using C2C12 cells. A horse meat hydrolysate less than 3 kDa (A4<3kDa) significantly increased the viability of C2C12 myoblasts against H2O2-induced cytotoxicity. Exposure of C2C12 myoblasts to lipopolysaccharide led to an elevation of cellular reactive oxygen species levels and mRNA expression of proinflammatory cytokines, including tumor necrosis factor-α and interleukin 6, and these effects were attenuated by A4<3kDa treatment. Additionally, A4<3kDa activated protein synthesis-related proteins through the protein kinase B/mechanistic target of rapamycin pathway, while decreasing the expression of activity and degradation-related proteins, such as Forkhead box O3, muscle RING finger protein-1, and Atrogin-1 in dexamethasone-treated C2C12 myotubes. Therefore, the natural material A4<3kDa has the potential of protecting against muscle atrophy, while further in vivo study is needed.

Pathological observation of mulberry heart disease of pigs in Korea (Mulberry heart disease의 병리학적 관찰)

  • Chung, Un-ik;Kim, Hong-jib;Eun, Gil-soo
    • Korean Journal of Veterinary Research
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    • v.37 no.3
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    • pp.601-606
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    • 1997
  • Mulberry heart disease was associated with vitamin E and selenium deficiency of pigs. Anatomical findings of this disease were hydropericadium, extensive patchy hemorrhage of epicardium, endocardium, and discoloration of dark red color of myocardium. In histological findings were characterized by acute myocardial degeneration, extensive hemorrhage, fibrinoid degeneration of arterioles, PAS positive material deposition of arterioles and capillary thrombosis. In affected herds, feed and serum tocopherol and selenium concentration were less than normal values.

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Analysis of Residual Fatty acid of the material for Reinforcement used on Edges of Lacquer ware inlaid box with Mother-of-pearl in Chŏson Dynasty (나전칠기함(螺鈿漆器函) 모서리 보강재료(補强材料)의 잔존지방산(殘存脂肪酸) 분석(分析))

  • Yu, Hei-sun
    • Conservation Science in Museum
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    • v.1
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    • pp.53-59
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    • 1999
  • Residual fatty acid of the basic material[Backgol] for reinforcement used on the edges of the lacquer ware inlaid box of Chosŏn Dynasty was analyzed. The result showed that it contained considerable amount of cholesterol. So it was confirmed to be animal material. Gas chromatography showed that its fatty acid composition is similar to that of sharkskin. On the basis of this analysis results, the damaged area of the object was restored by using sharkskin as a material for reinforcement.

A Study on the Environment-friendly Material of North American Indian Costume -Focused on Traditional Costume- (북미인디안의 환경친화적인 복식 소재에 관한 연구 -전통복식을 중심으로-)

  • 한명숙;박부진;남기선
    • The Research Journal of the Costume Culture
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    • v.7 no.1
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    • pp.13-26
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    • 1999
  • The purpose of this study was to identify the origin of diversity of costume material caused by environmental difference. For this purpose, this research involved theoretical studies and studies based on historical data obtained from previous related studies. The common truth was proved that environmental including the geographical distribution of plants and animals determines costume material. Also, It was found that the unique traditional costume styles were developed through unique combination of costume material and their culture. The major results of this study are as follow : 1. The traditional costume of North American Indian was well-developed and closely related to geographical distribution of plants and animals. Also, their costume was so diverse that it could not be categorized. 2. The traditional costume of North American Indian showed ideal har mony between nature and human being surrounding environment into their costume. 3. The application of plants and animal materials into their costume was one of the environment-friendly human activity and it seems to give us an important message.

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