• BMB Reports
• /
• 제39권6호
• /
• pp.737-742
• /
• 2006

Open reading frame 60 of Bombyx mori nucleopolyhedrovirus (Bm60) is located between 56,673 and 57,479 bp in the BmNPV genome which encodes 268 amino acid residues with predicted molecular weight of 31.0 kDa. Bm60 and its homologues have been identified in 11 completely sequenced lepidopteran NPVs. The transcript of Bm60 was detected by RT-PCR at 18-72 h post-infection (p.i.), while the corresponding protein could be detected at 24-72 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm60. The expression of Bm60 was inhibited in the presence of Ara-c, an inhibitor of viral DNA synthesis. These results together indicated that Bm60 was a late gene. The size of Bm60 product was found to be a 31 kDa in BmNPV-infected BmN cells, consistent with predicted molecular weight. Immuno-fluoresence analysis showed that the Bm60 product was first detected in the cytoplasm at 24 h p.i and also located in nucleus during later infection. In conclusion, the available data suggest that Bm60 is a functional ORF of BmNPV and encodes a 31kDa protein expressed in the later stage of infection cycle.

• 한국수의병리학회:학술대회논문집
• /
• 한국수의병리학회 2002년도 추계학술대회초록집
• /
• pp.145-145
• /
• 2002

Chronic viral infection has been reported to cause a range of hepatic lesion, including hepatic fibrosis, cirrhosis and hepatocellular carcinoma (HCC) in a wide variety of animal species. Woodchucks (Marmota monax) chronically infected with woodchuck hepatitis virus (WHV) develop similar progressive hepatic inflammatory and neoplastic lesions that are remarkably similar to those associated with HBV infection in humans. Twenty two-month-old offspring from woodchucks (Marmota monax) experimentally infected with woodchuck hepatitis virus, were purchased. One randomly chosen animal was autopsied. The liver exhibits marked cirrhotic changes characteristic of the pre-transformation phase of WHV. We believe that this may represent a new suitable and cost-effective model for the disease processes associated with hepadnaviruses in a number of other species, most notably Hepatitis B virus infection in man.

• 대한수의학회지
• /
• 제57권4호
• /
• pp.235-243
• /
• 2017

Salmonella (S.) Typhimurium is highly contagious, and its infection may rapidly spread within pig populations of herd. According to the survey (1,191 pigs) from 2003 to 2012, 155 pigs (13.0%) were diagnosed as salmonellosis in Jeju. Major porcine salmonellosis cases (88.4%) were concentrated in 4- to 12-week-old weaned pigs, but 6 pigs (3.9%) under 4 weeks old were also diagnosed. Based on the histopathologic examinations, ulcerative enteritis (63.9%) in the large intestine and/or paratyphoid nodules formation (57.4%) in the liver were most prevalent lesions in porcine salmonellosis. Single infection of S. Typhimurium and mixed infection with more than 2 pathogens were detected in 38 (24.5%) and 117 (75.5%) in pigs, respectively. Co-infections of Porcine reproductive and respiratory syndrome virus and Porcine circovirus type 2 were very common in porcine salmonellosis in Jeju and detected in 84 (54.2%) and 59 (38.1%) pigs, respectively. Based on the serotyping tests using 41 bacterial isolates, S. Typhimurium and S. Rissen were confirmed in 39 (95.1%) and 2 (4.9%) cases, respectively.

• 대한수의학회지
• /
• 제27권1호
• /
• pp.85-91
• /
• 1987

For the development of the specific pathogen free (SPF) or germ free laboratory animals, a parasitological survey was carried out and numerous pinworms were collected from the large intestines and caeca of the host animal Mus musculus alba. The pinworms collected from the laboratory albino mice were identified as Aspiculuris tetraptera, Syphacia muris and S. obvelata and classified into the Family Oxyuridae, Superfamily Oxyuroidea, Order Ascaridida. The overall infection rate of the pinworms was revealed as high as 64.8%(A. tetraptera 31.0%; S. muris 32.4% and S. obvelata 22.5%) consisting of the single species infection 47.9%, the double species infection 12.7% and the triple species infection 4.2%.

• Asian-Australasian Journal of Animal Sciences
• /
• 제6권3호
• /
• pp.343-345
• /
• 1993

Eight hundred and seventy on goats from Naikhongchari FSR site in Bandarban district were examined for gastro-intestinal nematodes. The parasites encountered in the region were Haemonchus Sp, Bunostomum Sp, Oesophagostomum Sp and Strongyloides Sp. The overall infection rate was 78.41%. Among various species found. Haemonchus Sp emerged as the most prevalent, although Oesophagostomum and Bunostomum were also found in this studies. The seasonal fluctuation in infection was assessed by monitoring the fecal egg count. The maximum values for the prevalence and overall mean EPG were observed after the heavy rain fall season and remained at a relatively high level from June to November. Haemonchus Sp and Oesophagostomum Sp appeared to be of major important parasites in the goats of this climatic zone which deserved proper treatment to attain higher production from goat.

• 한국어병학회지
• /
• 제17권3호
• /
• pp.213-216
• /
• 2004

To confirm the possibility of a wild marine crab, Gaetice depressus, as a carrier for white spot syndrome virus (WSSV) and to develop an alternative experimental model for WSSV in winter season, the susceptibility of the crab to WSSV was assessed by artificial challenge and subsequently tested for infection by PCR assay. The results revealed that the crabs were as highly susceptible as penaeid shrimps. WSSV caused 100% mortality in G. depressus within 16 days after intramuscular injection. The presence of WSSV in the moribund crabs was confirmed by PCR and was found in gills and muscle tissue. These results suggest that G. depressus can be naturally infected by WSSV via moribund shrimps, and can act as a potential carrier of WSSV. In addition, G. depressus can be used as an alternative experimental animal for WSSV.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권11호
• /
• pp.1535-1542
• /
• 2010

Xenotransplantation is a hot topic currently, since the demand for diverse organs is increasing in patients. Among many species, pigs are suitable animals for xenotranplantation as they share many anatomical and physiological characteristics with humans. This review article provides an overview of porcine xenotransplantation and the rejection of pig xenotransplants in primates, and use of genetically modified and cloned pigs in xenotransplantation. It also highlights major target organs in porcine xenotransplantation and virus infection in xenotransplantation.

• Asian-Australasian Journal of Animal Sciences
• /
• 제9권5호
• /
• pp.597-601
• /
• 1996

Twelve Philippine does of 3.5-5.0 years old were used in this study. They were divided into three groups, $T_1$, $T_2$ and $T_3$. Four animals were randomly allocated to each group. Before infection, they were dewormed and housed in individual pens with concrete floors. They were provided with a uniform management. They were infected orally with a single dose of three levels (0, 15,000 and 30,000) of infective Haemonchus contortus larvae. Blood samples were collected from the jugular vein of each animal at fortnightly intervals for 17 fortnights. Hemoglobin (Hb) concentration was determined using hemoglobinometer and packed cell volume (PCV) by microhematocrit methods. Animals in infected groups showed significantly (p < 0.05) lower hemoglobin values than the control except during fortnight 1 for group 2 and fortnights 1, 2, 5, 6 and 7 for group 3. Hemoglobin concentration did not significantly (p > 0.05) differ between the animals in infected groups throughout the sampling period except fortnight 2. The PCV values of animals in infected groups were significantly (p < 0.05) lower than the control for most of the sampling periods. The PCV values of animals in group 2 did not significantly (p > 0.05) differ from group 3.

• Parasites, Hosts and Diseases
• /
• 제56권3호
• /
• pp.281-285
• /
• 2018

Cryptosporidium is the most common protozoan that can infect a wide range of animals, including mammals and birds. Avian Cryptosporidium spp. can cause enteric and respiratory diseases which can be fatal in birds and some species are zoonotic. Companion birds have the potential as reservoir due to their close contact with humans. Pet shops are the major source of companion birds. However, few reports are available regarding Cryptosporidium spp. infection among companion birds kept in pet shops. The present study reports the prevalence and molecular characteristics of Cryptosporidium spp. among companion birds kept in pet shops in Japan. A total of 265 fresh fecal samples were obtained from birds kept in 4 pet shops; these birds belonged to 41 species in 3 bird orders. A nested polymerase chain reaction (PCR) assay targeting the small subunit rRNA gene was employed for the detection of Cryptosporidium spp. A total of 24 samples (9.1%) were positive, and Cryptosporidium spp. were detected from all pet shops. The prevalence of Cryptosporidium spp. in each of the bird orders was 6.5% (10/153) in Psittaciformes, 14.4% (13/90) in Passeriformes, and 4.5% (1/22) in Galliformes. Based on sequence analysis, 13 (54.2%) isolates were classified to C. galli, 8 (33.3%) were avian genotype III, and the remaining 3 (12.5%) were C. baileyi. No infection with zoonotic C. meleagridis and no coinfection with multiple Cryptosporidium spp. and/or genotypes were observed. The zoonotic potential of Cryptosporidium spp. infecting companion birds kept in pet shops in Japan is likely to be low.

• 대한수의학회지
• /
• 제42권1호
• /
• pp.109-113
• /
• 2002

This is the first case report of Stephanurus dentatus infection of a feral pig in Korea. In late April, 2000, a weakened feral pig was caught by blow gun from a very low level mountain near the Gyeongsang National University. We autopsied the feral pig in the laboratory of veterinary anatomy at the College of Veterinary Medicine. A total of 27 adult parasites, 11 females and 16 males, and numerous eggs were observed from the cysts formed in the perirenal tissues and ureters. The average size of males was $25.1{\pm}3.2mm$ long and of the females was $34.2{\pm}2.9mm$. The worms were stout, the females being about 2mm broad, and the internal organs were partly visible through the cuticle. The shape of thin-shelled eggs found in the cysts of perirenal tissues and ureter was ellisoidal and oval, and measured $40{\sim}65{\times}90{\sim}115{\mu}m$. The adult parasites were found in cysts which varied from 0.6 to 4cm in diameter, each cyst usually containing a pair of adult worms embedded in green pus. The ureter was thickened and almost occluded, with consequent hydronephrosis.