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Terminal solid solubility of hydrogen of optimized-Zirlo and its effects on hydride reorientation mechanisms under dry storage conditions

  • Kim, Ju-Seong;Kim, Tae-Hoon;Kim, Kyung-min;Kim, Yong-Soo
    • Nuclear Engineering and Technology
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    • v.52 no.8
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    • pp.1742-1748
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    • 2020
  • TSSD, TSSP, and TSSP2 of hydrogen for optimized-Zirlo (Zirlo™) alloy were measured by DSC in the range of 53-457 wppm. Solvus curves of the TSSs are derived and proposed in this study. The results show that the temperature gap between TSSD and TSSP solvus lines of Zirlo™ are similar to those of other zirconium alloys, but another gap between the TSSD and TSSP2 line differs significantly. In particular, the TSSP2 solvus line becomes closer to the TSSD solvus line than to TSSP unlike Zircaloy-4, so ΔTTSSD-TSSP2 of Zirlo™ decreases with decreasing temperature. This implies that hydride reorientation can take place more significantly in Zirlo™ than in Zircaloy-4, and the limited temperature variation of 65 ℃ during the vacuum drying and the cooling-down process may not be sufficient to prevent the triggering of hydride reorientation in Zirlo™ cladding under long-term dry storage.

Production of A Monoclonal Antibody (MAb) Against a Thermal Stable-Soluble Protein in Mackerel and Confirmation of the Properties for the MAb (고등어 어육 중 열안정성 단백질에 특이한 단클론성 항체 개발과 특성 확인)

  • Lee, Jeong-Eun;Kim, Jeong-Sook;Chung, Duck-Hwa;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.32 no.1
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    • pp.75-81
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    • 2017
  • For people who have a food allergy the only way to manage the allergy is to avoid the food allergen. The mackerel is one of the major food allergens, but no immunoassay for the rapid and simple detection of mackerel has been reported. The objectives of this study are to develop and characterize monoclonal antibodies (MAbs) specific to mackerel using thermal stable-soluble proteins (TSSP) as an immunogen and to characterize the MAbs by indirect enzyme-linked immunosorbent assay (iELISA). The mice immunized with mackerel TSSP and showing high titer were used for cell fusion and cloning. The characterization of MAbs produced from hybridoma cells obtained was confirmed by indirect ELISA and western blot. Four MAbs were confirmed to be specific to mackerel without cross-reaction to other marine products and livestock products in the both methods. The iELISA and western blot based on the MAbs can sensitively detect 1% mackerel protein in other marine products. These results support that immunochemical methods based on the MAb produced could be used as rapid means to detect low levels of mackerel and to identify mackerel adulterated in food.

A Study on the Space Charge Measurement Technique and Carrier Polarity of Insulating Materials on Power Cable (전력케이블용 절연재료의 캐리어 극성 및 공간전하 측정기술에 관한 연구-PE-EVA에서의 하전입자의 거동)

  • 국상훈;박중순;강용철;권영수
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.41 no.2
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    • pp.185-191
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    • 1992
  • In this paper, it is attempted to distinguish the charged particles and to judge the polarity by the use of Thermally Stimulated Current(TSC) and Temperature Gradient Thermally Stimulated Surface Potential Measurement(TG-TSSP)with experimental insulation material XLPE-EVA for power cables which is made by blending cross-linked polyethylene(XLPE) and ethylene-vinylacetate copolymer(EVA). In addition, it is performed to investigate the effect of EVA blending. From the experimental results, it is known that for the case of XLPE-EVA blended experimental material, the generation of space charged electric field is not obtained in the high temperature region due to the obatruction of the injection of trapping carrier by the electron and the positive hole.

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Three-dimensional numerical simulation of hydrogen-induced multi-field coupling behavior in cracked zircaloy cladding tubes

  • Xia, Zhongjia;Wang, Bingzhong;Zhang, Jingyu;Ding, Shurong;Chen, Liang;Pang, Hua;Song, Xiaoming
    • Nuclear Engineering and Technology
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    • v.51 no.1
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    • pp.238-248
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    • 2019
  • In the high-temperature and high-pressure irradiation environments, the multi-field coupling processes of hydrogen diffusion, hydride precipitation and mechanical deformation in Zircaloy cladding tubes occur. To simulate this hydrogen-induced complex behavior, a multi-field coupling method is developed, with the irradiation hardening effects and hydride-precipitation-induced expansion and hardening effects involved in the mechanical constitutive relation. The out-pile tests for a cracked cladding tube after irradiation are simulated, and the numerical results of the multi-fields at different temperatures are obtained and analyzed. The results indicate that: (1) the hydrostatic stress gradient is the fundamental factor to activate the hydrogen-induced multi-field coupling behavior excluding the temperature gradient; (2) in the local crack-tip region, hydrides will precipitate faster at the considered higher temperatures, which can be fundamentally attributed to the sensitivity of TSSP and hydrogen diffusion coefficient to temperature. The mechanism is partly explained for the enlarged velocity values of delayed hydride cracking (DHC) at high temperatures before crack arrest. This work lays a foundation for the future research on DHC.

Development of an Indirect Enzyme-Linked Immunosorbent assay for Rapid Detection of Adulteration of Food Allergen Mackerel in Processed Marine Foods (수산가공식품 중 알레르겐 고등어 혼입여부 신속 검출을 위한 간접효소면역분석법의 개발)

  • Lee, Jeong-Eun;Kim, Ah-Yoon;Kim, Sol-A;Kim, Hyo-In;Park, Ji-Hye;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.33 no.3
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    • pp.185-192
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    • 2018
  • The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ${\geq}0.3$ O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.