• 제목/요약/키워드: Anagrapha falcifera nuclear polyhedrosis virus

검색결과 2건 처리시간 0.014초

Biochemical Analysis of Anagrapha falcifera NPV Attachment to Spodoptera frugiperda 21 Cells

  • PARK, JIN O;JAI MYUNG YANG;IN SIK CHUNG
    • Journal of Microbiology and Biotechnology
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    • 제9권3호
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    • pp.361-364
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    • 1999
  • The binding characteristics of Anagrapha falcifera nuclear polyhedrosis virus (AtNPV) to Spodoptera frugiperda 21 (Sf21) cells were investigated. The cells displayed an affinity of 4.7×10/sup 10/M/sup -1/ with about 3,300 binding sites per cell. The biochemical nature of the AfNPV-binding sites on the cell surface was also partially identified. Our findings suggest that the binding-site moiety has a glycoprotein component, but that the direct involvement of oligosacccharides containing N-acetylglucosamine or sialic acid residues in binding is unlikely, and that AfNPV entry into Sf21 cells may be via receptor-mediated endocytosis.

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Optimization of Propagation of Anagrapha falcifera Nuclear Polyhedrosis Virus in Spodoptera Frugiperda 21 Cells

  • Lee, Jong-Min;Chang, Kyung-Hwa;Park, Jin-O;Park, Jong-Hwa;Hwang, In-Sook;Lee, Youn-Hyung;Yang, Jai-Myung;Chung, In-Sik
    • Journal of Microbiology and Biotechnology
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    • 제10권5호
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    • pp.728-732
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    • 2000
  • Propagation of Anagrapha falcifera nuclear polyhedrosis virus(AfNPV) was investigated using well-plates and split-flow air-lift bioreactors. In well-plate experiments, the effects of pH, cell density at a point of infection, serum concentration, DEAE-dextran, and lipid on virus propagation were all closely examined. The AfNPV titer in well-plates was optimal at pH 6.8 and $3{\times}10^6$ cells/$cm^2$. The virus titer was not dramatically affected when the fetal bovine serum concentration was reduced from 10% to 5%. The addition of cholesterol at AfNPV infection of Sf21 cells enhanced the virus titer, whereas the addition of DEAE-dextran did not improve the titer. The AfNPV titer ($3.8{\times}10^7$ $TCID_{50}/ml$) at optimized conditions for well-plate experiments was 2.5-fold higher than for the control. In bioreactor experiments, the AfNPV titer showed its maximum level at air flow rates of 20-40 ml/min. In a split-flow air-lift bioreactor, AfNPV titer ($2.3{\times}10^7\;TCID_{50}/ml$) was 1.5-fold higher than the control when the culture was at pH 6.8 and supplemented with 0.34 mM cholesterol.

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