• Microbiology and Biotechnology Letters
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• v.27 no.3
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• pp.230-235
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• 1999

This study was intended to develop a new rice product by the fermentation of saccharified rice solution using Bifidobacterium and to select an appropriate reducing agent to provide the anaerobic condition for the growth of Bifidobacterium during fermentation. The enhancement of the growth of Bifidobacterium in saccharified rice solution was achieved by the treatment of reducing agents such as ascorbic acid and cysteine. The physical and chemical properties of the fermented product were evaluated, and the effect of the reducing agents were compared between ascorbic acid and cysteine. The fermented product with the addition of ascorbic acid shows the lower pH and the higher titratable acidity comparing the product with the addition of cysteine. This indicated that ascorbic acid was more appropriate reducing agent than cysteine for the fermentation of the saccharified rice solution. The number of viable Bifidobacterium in the fermented product with the addition of ascorbic acid(2.2$\times$108~3.4$\times$108CFU/ml) was greater than that with the addition of cysteine (8$\times$107~2.8$\times$108CFU/ml). Ascorbic acid supplement also contributed better sensory properties, such as flavor, taste and overall acceptibility than cysteine supplement did.

• Environmental Engineering Research
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• v.10 no.4
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• pp.174-180
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• 2005

Nano-sized iron was synthesized using borohydride reduction of $Fe^{3+}$ in aqueous solution. A wide range of synthesis conditions including varying concentrations of reagents, reagent feeding rate, and solution pH was applied in an aqueous system under anaerobic condition. The reactivity of nano-sized iron from each synthesis was evaluated by reacting the iron with TCE in batch systems. Evidence obtained from this study suggest the reactivity of iron is strongly dependent on the synthesis solution pH. The iron reactivity increased as solution pH decreased. More rapid TCE reduction was observed for iron samples synthesized from higher initial $Fe^{3+}$ concentration, which resulted in lower solution pH during the synthesis reaction. Faster feeding of $BH_4^-$ solution to the $Fe^{3+}$ solution resulted in lower synthesis solution pH and the resultant iron samples gave higher TCE reduction rate. Lowering the pH of the solution after completion of the synthesis reaction significantly increased reactivity of iron. It is presumed that the increase in the reactivity of iron synthesized at lower pH is due to less precipitation of iron (hydr)oxides or less surface passivation of iron.

• Environmental Engineering Research
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• v.13 no.4
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• pp.177-183
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• 2008

Ex-situ reductive dechlorination of carbon tetrachloride (CT) by iron sulfide in a batch reactor was characterized in this study. Reactor scaled-up by 3.5 L was used to investigate the effect of reductant concentration on removal efficiency and process optimization for ex-situ degradation. The experiment was conducted by using both liquid-phase and gas-phase volume at pH 8.5 in anaerobic condition. For 1 mM of initial CT concentration, the removal of the target compound was 98.9% at 6.0 g/L iron sulfide. Process optimization for ex-situ treatment was performed by checking the effect of transition metal and mixing time on synthesizing iron sulfide solution, and by determining of the regeneration time. The effect of Co(II) as transition metal was shown that the reaction rate was slightly improved but the improvement was not that outstanding. The result of determination on the regeneration time indicated that regenerating reductant capacity after $1^{st}$ treatment of target compound was needed. Due to the high removal rates of CT, ex-situ reductive dechlorination in batch reactor can be used for basic treatment for the chlorinated compounds.

• Journal of Korean Society on Water Environment
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• v.26 no.3
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• pp.468-473
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• 2010

The purpose of this study is to derive the correlation between the Specific Phosphorus Release Rate (SPRR), Specific Phosphorus Uptake Rate (SPUR) and Specific Oxygen Uptake Rate (SOUR) at various Sludge Retention Time (SRT) condition in the A/O process. The laboratory scale reactor was operated on various SRT (10 day, 20 day, 30 day, 40 day). In this study, the SPRR, SPUR and SOUR tended to decrease with the SRT increase. Empirical equations was be obtained $y=4.54E-006x^2+0.0007x-0.0315$, $R^2=0.925$ (SOUR vs. SPRR) and $y=3.22E-006x^2+0.0004x-0.0173$, $R^2=0.928$ (SOUR vs. SPUR) from the relationship between SRT, SPRR and SPUR and SOUR. Therefore, the anaerobic tank design based on the research result such as SPRR, SPUR of a phosphorus design and SOUR would be possible.

• Proceedings of the Korean Environmental Health Society Conference
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• 2003.06a
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• pp.199-203
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• 2003

This study was carried out to investigate the removal of 2,4,6-trinitrotoluene, nutrients such as nitrogen, phosphorous compounds in the wetland microcosms. Microcosm study indicated that TNT nutrients were more lastly reduced in the anaerobic condition. The major reductive transformation products included 2,4diamino-6-nitrotoluene (2,4-DANT) 2,6diamino-4-nitrotoluene (2,6-DANT) 4diamino-2,6-nitrotoluene (4-ADNT) 2diamino-4,6-nitrotoluene (2-ADNT). The experimental results for nitrogen removal showed that denitrification kom NO$\sub$3//$\^$-/ to NH$_3$ was dominant process at the bottom of marsh, but nitrification from NH$_3$ to NO$\sub$3//$\^$-/ was dominant on the top of marsh.

• Journal of Environmental Science International
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• v.16 no.1
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• pp.45-53
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• 2007

This study was carried out to investigate the variation of organic, nitrogen and phosphorus in $(AO)_2$ SBBR process according to the variation of operating cycle at the high TOC concentration. The operation time in anoxic (anaerobic) time to oxic time was 1:1. Three lab-scale SBBRS were fed with synthetic wastewater based on glucose as carbon source, The variation of total TOC removal was similar each other irrespective of operation time, however, the TOC concentrations in SBBRs showed a little difference according to the operating condition. In SBBR, complete nitrification was not occurred at all reactors, however, R3 showed a higher nitrification than R2. And in SBBR, the variation of operating time more affected at phosphorus removal than nitrogen removal. R2 which had the shortest time at the 1st aeration time showed the lowest phosphorus release and uptake efficacy.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.6
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• pp.125-130
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• 2014

In recent years, the IT conversions have rapidly developed along the development of the information technologies and devices. The overuse of IT devices caused by this trend has weakened the physical condition of children these days, compared to the past, therefore a study for this problem is necessary. This study intends to improve the health of children, easy to lack physical activities caused by the addiction to PC and smart phones, by proposing a health care application for them using Kinect. This application will introduce some simple aerobic/anaerobic exercises that could be done in home and will help children to forget the boredom by exercising alone and to coach them by using it.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1979.10a
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• pp.246.1-246
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• 1979

A glucose-limited “pH-stat” continuous culture study of Lactobacillus bulgaricus NLS-4 in an anaerobic condition showed the marked effects of environmental pH on end products, fermentation blances and bioenergetic aspects of the organism. Lactic acid was the major end product of fermentation with minor products, such as acetic acid, formic acid and ethanol throughout the pH range tested. In acidic conditions below pH 6.5, a typi-cal pattern of homofermentation was revealed whereas in alkaline conditions, the metabolic pattern was changed from homofermentation to heterofermentation and led to acquire much energy. This metabolic change was likely due to the pH-dependent lactate dehydrogenase activity. Molar growth yields (Yglc=35.5-44.4) and YATP, $18.5\pm2.5$ in average which was 80％ higher than the value ever postulated seemed to be accounted for less requirement of maintenance energy of the organism in the culture conditions.

• Journal of the Korea Organic Resources Recycling Association
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• v.9 no.1
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• pp.127-133
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• 2001

It can be known that from leachate generated in the initial stage of landfill there are a lot of undecomposed orgainc materials, its sulfur component reduces to sulfide ion by sulfur reducing microorgarnisms as an anaerobic digestion proceeds, the sulfide ion makes the leachate discolor to black by forming metal sulfide sol, on condition that much more equivalent of sulfide ion than that of metal ion is present, and the metal sulfide sol can be generated to the precipitates by forming black-colored particulates. Therefore, we can confirm the important possibility for the economic and efficient treatment of leachate that it can be passivated, provided that much more equivalent of sulfide ion is present in the reaction of sulfide ion and metal ion.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.77-86
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• 2011

Alcohol dehydrogenase (E.C.1.1.1.1) is an enzyme present in higher plants involved in the anaerobic fermentation pathway that catalyzes the reduction of pyruvate to ethanol, resulting in continuous $NAD^+$ regeneration. It also plays an important role in many plant developments including tolerance to anoxia condition. Here, a cDNA clone encoding alcohol dehydrogenase (ADH) was isolated from Chinese cabbage (Brassica rapa) seedlings. The gene named Bradh1 had a total length of 1,326 bp that contains a single open reading frame of 1,140 bp. The predicted protein consists of 379 amino acid residues with a calculated molecular mass of 41.17 kDa. Expression pattern analysis revealed a tissue-specific expressing gene in different tissues and strongly expressed in the shoot, roots and seeds of Chinese cabbage. Agrobacterium transformation of full-length cDNA Bradh1 into rice Gopumbyeo showed high efficiency. Furthermore, induction of ADH in transgenic rice enhanced tolerance to anaerobiosis stresses and elevated mRNA transcripts. The overexpression of Bradh1 in rice increases germination under anaerobiosis stresses, implying the possibility of developing new varieties suited for direct seeding or flood-prone rice field.