• The Korean Journal of Physiology and Pharmacology
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• v.14 no.2
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• pp.71-75
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• 2010

To investigate the intestinal absorption of a fibrinolytic and proteolytic lumbrokinase extracted from Eisenia andrei, we used rat everted gut sacs and an in situ closed-loop recirculation method. We extracted lumbrokinase from Eisenia andrei, and then raised polyclonal antibody against lumbrokinase. Fibrinolytic activity and proteolytic activity in the serosal side of rat everted gut sacs incubated with lumbrokinase showed dose- and time-dependent patterns. Immunological results obtained by western blotting serosal side solution using rat everted gut sacs method showed that lumbrokinase proteins between 33.6 and 54.7 kDa are absorbed mostly by the intestinal epithelium. Furthermore, MALDI- TOF mass spectrometric analysis of plasma fractions obtained by in situ recirculation method confirmed that lumbrokinase F1 is absorbed into blood. These results support the notion that lumbrokinase can be absorbed from mucosal lumen into blood by oral administration.

• Journal of Environmental Science International
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• v.12 no.2
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• pp.119-124
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• 2003

An effective grafting method for jujube nursery trees was developed to shorten the operation time, improve the percentage of "takes" and shoot emergence, and form the better graft union. Out of 7 grafting methods the splice, modified scion Ono graft and the bark graft showed relatively short operation time during the grafting operation comparing to the modified rootstock Ono graft or the chip budding. Among these methods, the bark graft demonstrated 100% "takes", but the modified scion Ono graft or the standard Ono graft showed 58.3% of "takes". The percentages of the vegetative shoot emergence were 100% fir the bark graft, 70% f3r the modified scion Ono graft and lower emergence percent for the rest grafting methods. The union tissue formation for the modified scion Ono graft, the bark graft, or the whip-and-tongue graft was significantly larger than the standard Ono graft or the splice graft. In a close examination of the cross sectional areas of the graft union formation through the microscope, the bark graft was superior, the standard Ono graft was inferior and rest of the grafting methods were intermediate.ing methods were intermediate.

• Molecules and Cells
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• v.45 no.11
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• pp.820-832
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• 2022

As a potential candidate to generate an everlasting cell source to treat various diseases, embryonic stem cells are regarded as a promising therapeutic tool in the regenerative medicine field. Cohesin, a multi-functional complex that controls various cellular activities, plays roles not only in organizing chromosome dynamics but also in controlling transcriptional activities related to self-renewal and differentiation of stem cells. Here, we report a novel role of the α-kleisin subunits of cohesin (RAD21 and REC8) in the maintenance of the balance between these two stem-cell processes. By knocking down REC8, RAD21, or the non-kleisin cohesin subunit SMC3 in mouse embryonic stem cells, we show that reduction in cohesin level impairs their self-renewal. Interestingly, the transcriptomic analysis revealed that knocking down each cohesin subunit enables the differentiation of embryonic stem cells into specific lineages. Specifically, embryonic stem cells in which cohesin subunit RAD21 were knocked down differentiated into cells expressing neural alongside germline lineage markers. Thus, we conclude that cohesin appears to control the fate determination of embryonic stem cells.

• Proceedings of the KIEE Conference
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• 1999.07c
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• pp.1314-1317
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• 1999

In this paper, we design an Adaptive Neuro-Fuzzy Inference System(ANFIS) Precompensator for the performance improvement of conventional proportional integral derivative (PID) controller that the governor system of power plant constantly maintains the load frequency of two-area power system. The ANFIS Precompensator is expressed as the membership functions of premise parameters and the linear combination of consequent parameters by Sugeno's fuzzy if-then rules using nonlinear input-output relation for the set point automatic modification maintaining conventional PID controller. The proposed compensation design technique is hoped to be satisfactory method overcome difficulty of exact modelling and arising problems by the complex nonlinearities of power system, and our design shows merit that is easily implemented by adding an ANFIS precompenastor to an existing PID controller without replacement.

• Natural Product Sciences
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• v.19 no.2
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• pp.192-200
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• 2013

As part of the research for the natural products about prostate-related disease, this study screened 159 plant species from 46 families, which included a total of 213 different kinds of local native plants and these plants were tested for the ability to inhibit LNCaP proliferation, an androgen-sensitive prostate cancer cell line, and DU145 proliferation, which is a more aggressive androgen-insensitive prostate cancer cell line. The results indicated that nineteen of 213 types of plants exhibited antiproliferative activity (cell viability < 30%, $500{\mu}g/mL$) on the growth of androgen-sensitive LNCaP cell lines, and five of them exhibited DU145 cell antiproliferative activity (cell viability < 30%, $500{\mu}g/mL$). The methanol extracts of Eurya emarginata (stems), Gleditsia japonica var. koraiensis (leaves), Photinia glabra (leaves) and Elaeagnus macrophylla (leaves) showed antiproliferative activity on both the androgen-sensitive LNCaP cells (cell viability < 30%) and androgen-insensitive DU145 cells (cell viability > 100%). The study also found that the methanol extracts of Styrax japonica (fruits), Aralia continentalis (leaves), Fagus crenata var. multinervis (stems), Thuja orientalis (stems) and Poncirus trifoliate (branches) presented the strongest activity and demonstrated potent antiproliferative activity on both cell lines (LNCaP and DU145 cell viability < 30%).

• Proceedings of the KIEE Conference
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• 2001.11a
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• pp.45-46
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• 2001

본 논문은 편광유지 광섬유 루프 미러를 이용한 센서 시스템의 제작과 이의 응용에 대한 것이다. 수동 검출법을 통하여 비교적 간편한 신호처리 방식을 갖는 센서 시스템을 제작하였고, Fabry-Perot 필터를 스캔하여 다중점 측정에 이용하였다. 또한 루프 미러의 온도 의존성을 이용하여 온도 센서로의 응용 가능성도 제시하였다.

• Journal of Korean Society for Quality Management
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• v.20 no.1
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• pp.11-21
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• 1992

The Purpose of this paper contribute to design the multistate Markov process for the reliability of a system when the transition-rates of each unit depend on the current state of the system. The system transition-rate matrix has the form of the kronecker sum of transition rate matrices for the units, is analyzed and investigated. As a result, the system which has the state-dependent units is detaily analyzed and introduced by the approach of an algorithm for the system transition-rate matrix based on the kronecker algebra.

• 한국전자현미경학회:학술대회논문집
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• 2001.05a
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• pp.32-32
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• 2001

• Proceedings of the KIEE Conference
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• 1997.07b
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• pp.468-470
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• 1997

This paper presents an estimation method of the number of partial discharge(PD) sources and their locations in power transformer. To identify multiple PD sources and their locations, the directions-of-arrival estimation algorithm is employed. The results of simulations shows that the proposed method could be accurately detect the number of PD sources and their locations in power transformer.

• 한국정보디스플레이학회:학술대회논문집
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• 2007.08a
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• pp.713-716
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• 2007

This paper presents the fabrication of an electro-luminescence display, which is whollyprinted on a flexible paper substrate. We expect that the printed EL display can be a powerful alternative for general advertisement which is printed only with media ink.