• Title/Summary/Keyword: Amylose

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Effects of High Amylose Starch on Lipid Metabolism and Immune Response in Rats (고아밀로오스전분의 섭취가 흰쥐의 지방대사 및 면역능력에 미치는 영향)

  • 설소미;방명희;최옥숙;김우경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.3
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    • pp.450-457
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    • 2003
  • This study was conducted to investigate the effects of high amylose corn starch consumption on plasma, liver and feces lipid profiles and immune responses in male Sprague-Dawley rats. Experimental animals were fed on diets containing the high amylose starch (HAS,0, 125, 250,500 g/kg diet) for 4 weeks. HAS intake did not affect on food intakes and food efficiency ratio. Final body weights were lowered in HA100 group than in control group. HAS intakes dose dependently increased the weights of cecum and excretion of feces per day, and decreased the pH of cecum contents. And HAS intakes significantly decreased the plasma total lipid, triglyceride and total cholesterol levels. But there were not significant differences total lipid, triglyceride and cholesterol concentrations in liver. The absolute and relative weights of thymus and spleen, plasma Is G and $C_3$ concentrations were unaffected by experimental diets. The splenocyte proliferations with low dose Con A (0.1$\mu\textrm{g}$/10 $\mu$L) as lower in HA25 group and HA50 group than in control group. These results demonstrate that high amylose corn starch intakes significantly improve lipid profiles in plasma.

Amylolytic Activity of Brown Rice and Black Rice during Germination (현미와 흑미의 발아과정 중 amylolytic activity)

  • Lee, Hyang-Mi;Im, Ji-Soon;Park, Jong-Dae;Kum, Jun-Seok;Lee, Hyun-Yu;Lee, Young-Tack
    • Korean Journal of Food Science and Technology
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    • v.45 no.3
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    • pp.333-338
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    • 2013
  • Enzymatic activity in germinated cereal grains is important for the saccharification of starch materials. This study was conducted to investigate the amylolytic activities of germinating brown rice and black rice that have different amylose contents. Brown rice and black rice were steeped at room temperature for 24 h and germinated at 20 and $30^{\circ}C$ for 1, 2, and 3 days. ${\alpha}$- and ${\beta}$-Amylase activities in normal brown rice increased very slightly during the 3-day germination period, but the enzymatic activities were slightly higher in low-amylose (waxy type) brown rice. Diastatic power (DP), a measure of starch-saccharifying enzyme, was higher in the germinating brown rice with low amylose than in those with normal amylose content. ${\alpha}$- and ${\beta}$-Amylase activities in black rice increased gradually during germination, and DP of low-amylose black rice appeared to be higher than that of normal brown rice. Amylase activities in brown rice and black rice germinated at $30^{\circ}C$ were higher than those germinated at $20^{\circ}C$. Compared to brown rice, the overall amylolytic activity of germinated black rice was observed to be higher than that of brown rice.

A Study on the Gelation of Starch (전분의 젤화에 관한 연구 -강남콩 조전분 및 정제전분의 이화학적 특성-)

  • 이진영;안승요;이혜수
    • Korean journal of food and cookery science
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    • v.3 no.1
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    • pp.47-53
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    • 1987
  • The physicochemical properties of kidney bean crude and refined starch were investigated. The results were as follows : Amylose content of refined starch was 77%. Blue values of crude and refined starch were 0.375 and 0.410, respectively. Ferricyanide numbers of crude and refined starch were 1.00 and 1.94, respectively, and alkalinumbers of crude and refined starch were 8.67 and 6.90, respectively. Amylose had molecular weight of 18067 and degree of polymerization was 112. Amylopectin had degree of branching of 3.7 per 100 glucose units and glucose units of 27 per segment of amylopectin. Water binding capacities of crude and refined starch were 202.1% and 169.4%, respectively. Both swelling powers of crude and refined starch were increased rapidly from $70^{\circ}C$ to $90^{\circ}C$ and their curves showed a single-stage pattern. The optical transmittance of 0.2% crude starch suspension was increased rapidly from $80^{\circ}C$ to $83^{\circ}C$ and that of 0.2% refined starch suspension was increased rapidly from $77^{\circ}C$ to $83^{\circ}C$. Brabender hot-paste viscosities of crude and refined starch at 6% and 8% concentation (solid basis) showed the similar amylogram patterns of c type with no peak vircosity.

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Effects of Low Temperature during Ripening on Amylose Content and Enzyme Activities Associated with Starch Biosynthesis in Rice Endosperm

  • Baek, Jung-sun;Jeong, Han-Yong;An, Sung-Hyun;Jeong, Jae-Heok;Lee, Hyen-Seok;Yoon, Jong-Tak;Choi, Kyung-Jin;Hwang, Woon-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.2
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    • pp.86-97
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    • 2018
  • The objective of this study was to determine the effects of low temperature on starch accumulation in rice grains. We used four major Japonica-type Korean rice cultivars as materials: Jinbu (JB), Junamjosaeng (JJ), Geumyoung (GY), and Hwawang (HW). Rice plants were moved into two phytotrons the day after heading. Temperatures in the two phytotrons were maintained at $19/29^{\circ}C$ (night/day) as the control, and $13/23^{\circ}C$ as the low temperature condition, both under natural daylight with a relative humidity of 65%. The ripening rates of JB and JJ showed no significant difference between the low temperature and control conditions at 45 days after heading (DAH). In contrast, the ripening rates of GY and HW were 86% and 57% lower than those of JB and JJ under the low temperature condition at 45 DAH, respectively. However, the ripening rates of these four varieties at 61 DAH (when accumulated temperature reached $1,100^{\circ}C$) under the low temperature condition were similar to those at 45 DAH under the control condition (JB, 94%; JJ, 97%; GY, 97%; HW, 88%). The total starch contents showed no significant difference between the control and low temperature conditions. However, the amylose contents in the cultivars were higher under the low temperature than under the control condition. The enzyme activities of starch biosynthesis were about 5-10 days slower in cultivars under the low temperature than under the control. The grain-filling rate showed significant correlations with the enzyme activities of SuSase ($r^2=0.70^{***}$), AGPase ($r^2=0.63^{***}$), UDPase ($r^2=0.36^{***}$), StSase ($r^2=0.51^{***}$), and SBE ($r^2=0.59^{***}$). In conclusion, although StSase activity was increased at $13/23^{\circ}C$ up to 20 DAH, there might not be enough time for SBE to synthesize amylopectin, thus affecting the amylose content of HW, which had the slowest grain filling rate. Notably, the decreased activity of SuSase and SBE and late increase in AGPase activity under the low temperature during the ripening stage are considered to be disadvantageous, as they delay ripening and increase the amylose content.

Study of Molecular and Crystalline Structure and Physicochemical Properties of Rice Starch with Varying Amylose Content (아밀로오스 함량이 다른 쌀 전분의 분자 및 결정 구조와 이화학적 특성)

  • You, Su-Yeon;Lee, Eun-Jung;Chung, Hyun-Jung
    • Korean Journal of Food Science and Technology
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    • v.46 no.6
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    • pp.682-688
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    • 2014
  • The in vitro digestibility and molecular and crystalline structures of rice starches (Seilmi, Dasan1, and Segoami) with differing amylose content were investigated. Segoami had the highest amylose content (30.9%), whereas Dasan1 had the lowest amylose content (21.2%). The molecular weight ($\bar{M}_w$) of amylose and amylopectin in Segoami was much lower than that of the other two rice starches. Segoami had the highest proportion (8.7%) of amylopectin short branch chains (DP 6-12) and the lowest proportion of B1 chains (DP 13-24). The relative crystallinity, intensity ratio of $1047-1022cm^{-1}$ (1047/1022) and gelatinization enthalpy followed the order: Segoami>Seilmi~Dasan1. Segoami showed substantially low pasting viscosity. Rapidly digestible starch (RDS), slowly digestible starch (SDS), and resistant starch (RS) contents showed the highest value in Seilmi, Dasan1, and Segoami, respectively. The expected glycemic index (eGI) of Segoami was lower than that of the other two rice starches. Overall results suggested that the digestibility of rice starch could be highly influenced by their molecular and crystalline structure.

Comparison of Some Characteristics Relevant to Rice Bread made from Eight Varieties of Endosperm Mutants between Brown and Milled Rice (8품종 변이체 벼의 현미 및 백미빵 가공성 비교)

  • Kang, Mi-Young;Koh, Hee-Jong;Han, Ji-Yeun
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.82-89
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    • 2000
  • Relationship among the properties of rice, such as amylose contents of endosperm starch, sugar content and amylogram characteristics, and processing properties for rice bread was studied. The amylose content of the rice cultivars decreased in order of Nampungbyeo, Whachungbyeo>Punchilmi(fl)>Nampung CB243> Whachung du-I, Nampung EM90>Whachung-chalbyeo>shr. Protein contents of rice tested in this study were almost same level, however, shr, the high sugar rice, showed the highest protein content scored as 8.2%. The study showed that the amylose contents of rice cultivars were negatively correlated with their protein contents. The starting temperatures for gelatinization of the flour of Punchilmi(fl) and Shurunken(shr) were low, however, in case of Whachungbyeo and Nampungbyeo plus their mutants derived from the both, the stickiness and the hardness of the flours were shown to be positively correlated with the amylose contents. In addition, loaf volume tested using sensory evaluation and overall quality showed the same tendency. Among the rice cultivars tested in this study, breads made from white rice had good qualities in bread making process than those made from brown rice. The bread made from Nampungbyeo was demonstrated to have highest score e in overall quality, as well as the lowest retrogradation index during storage at $4^{\circ}C$

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Amylose, Tocopherol, Free Sugar and Fatty Acid Content in Selected Mutant Lines of Oryza sativa cv. Shindongjin

  • Cho, Yoo-Hyun;Lee, Sok-Young;Kim, Seong-Min;Yu, Jae-Woong;Lee, Jung-Ro;Hong, Ha-Cheol;Kim, Jung-Bong;Ma, Kyung-Ho;Kwon, Taek-Ryun;Kang, Hee-Kyoung;Lee, Gi-An;Gwag, Jae-Gyun;Kim, Tae-San;Park, Yong-Jin
    • Journal of Crop Science and Biotechnology
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    • v.11 no.3
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    • pp.181-186
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    • 2008
  • To assess the potential as biofortified rice varieties, new endosperm and grain mutant lines were selected from $M_4$ generation seeds of the rice cultivar Shindongjin, which were either $\gamma$-irradiated or treated with N-methyl-N-nitrosourea(MNU) and lipid, sugar, and tocopherol content were analyzed. Amylose content in non-waxy mutants ranged from 8.8% in SM-4, a dull-type mutant, to 29.5% in SM-51, compared to 18.9% in the parental variety, Shindongjin. SM-23, a floury-type mutant, contained 0.09 ${\mu}g/g$ $\alpha$-tocopherol(40.9% of total tocopherol), was three times higher than in the parental variety. SM-32, a giant embryo-type mutant, had a 2.2-fold higher total tocopherol content, 2.1-fold higher $\alpha$-tocopherol, and 5.5-fold higher $\delta$-tocopherol content(47.3% of total tocopherol) than the parental variety(0.13 ${\mu}g/g$). Total free sugar content was elevated in all selected mutants and 1.2-8.6 times higher than in the parental variety(11.38 ${\mu}g/g$). These increased sugar levels were due to increase in sucrose concentration. SM-23(floury-type mutant) and SM-51(high amylose-type mutant) had 4.6 and 7.0 times more sugar, respectively, than the parental variety(11.38 ${\mu}g/g$). With relatively high concentrations, most mutants showed elevated fatty acid content in the SM 32(giant embryo-type) and SM-51(high amylose-type) mutants, at 124.56 and 89.59 mg/g, respectively. All selected mutants displayed valuable characteristics for the development of new varieties in rice-breeding programs.

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pH, Moisture, Solid, Total Sugar, Amylose, Viscosity, Spreadability and Color of Puffed Rice Powder Added Tarakjuk (팽화미분 첨가에 따른 타락죽의 pH, 수분, 고형분, 총당, amylose, 점도, 퍼짐성 및 색도 변화)

  • Kim, Ji-Young;Kim, Ju Bong;Yi, Young Hyoun
    • Food Engineering Progress
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    • v.13 no.2
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    • pp.159-163
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    • 2009
  • The pH, moisture, solid, total sugar, amylose, viscosity, line spread and color of Tarakjuk containing puffed rice powder were investigated. The pH of rice Tarakjuk was higher than that of puffed rice powder. Also, samples with whole milk powder were higher than fresh milk (p<0.05). The highest moisture contents, 84.56% was observed in rice Tarakjuk prepared with whole milk powder, while lowest 81.04% in puffed rice powder sample with fresh milk (p<0.05). Samples with lower moisture contents resulted in greater solid contents (p<0.05). Higher total sugar contents were noticed in cooked samples than uncooked ones, however no differences were found among cooked ones (p<0.05). Amylose contents of puffed rice powder Tarakjuk were higher than rice sample (p<0.05). The rice Tarakjuk showed higher viscosity and lower spreadability (p<0.05). Higher "L" value was observed in rice. Highest "a" values were shown in puffed rice powder and lowest value in rice with fresh milk (p<0.05). The "b" value tended to be similar to "a" value.

Effect of Nitrogen Fertilizer Rates on Rice Quality in Mid-mountainous Area (질소시비량이 중산간지 고품질 쌀 생산에 미치는 영향)

  • Kang J.R.;Kim J.T.;Beg I.Y.;Kim J.I.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.spc1
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    • pp.37-40
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    • 2005
  • In order to find out better cultivation practices in mid-mountainous area to produce high quality rice, an experiment involving seven nitrogen fertilizer rates and three varieties was carried out, during past two years$(2003\~2004)$. There was an interaction effect on palatability of milled rice between nitrogen fertilizer rate and variety. The palatability of Junambyeo grown by the treatment of nitrogen fertilizer yale wasn't different across the rates significantly, but Hwayongbyeo's palatability grown by 0 kg/ha nitrogen treatment was better than 50, 70,170 kg/ha, and Sangmibyeo's palatability grown by 70 kg/ha nitrogen treatment was better than 90, 110, 140, 170 kg/ha. Amylose content of milled rice was showed without a significant difference between nitrogen fertilizer rate, but in between variety, the amylose content of Sangmibyeo was lower than Hwayongbyeo's, also Hwayongbyeo's amylose content was lower than Junambyeo's. Protein content of milled rice was showed an interaction effect between nitrogen fertilizer rate and variety. The protein content of Hwayongbyeo and Junambyeo grown by the treatment of nitrogen fertilizer rate wasn't different across the fertilizer yale significantly, but Sangmibyeo's protein content grown by 0 kg/ha nitrogen treatment was lower than the rest of treatments, and it's protein content grown by 50, 70, 90 and 110 kg/ha nitrogen treatment was lower than 140 and 170 kg/ha. Bead rice yield of milled rice grown by lower nitrogen fertilizer rate, was lower than that grown by higher nitrogen fertilizer rate. In a conclusion gathering all above the results to produce high quality rice in Mid-mountainous area, the applicative nitrogen fertilizer rate was 70 kg per ha for Sangmibyeo, 110 kg/ha for Hwayongbyeo and 140 kg/ha for Junambyeo, in considering with head rice yield, palatability, amylose${\cdot}$protein content and productivity.

Evaluation of Quality Characteristics and Definition of Utilization Category in Korean Potato (Solanum tuberosum L.) Cultivars (국내산 감자(Solanum tuberosum L.)의 품종별 품질특성 평가 및 용도구분)

  • Lee, Yeh-Jin;Jeong, Jin-Cheol;Yoon, Young-Ho;Hong, Su-Young;Kim, Su-Jeong;Jin, Yong-Ik;Nam, Jeong-Hwan;Kwon, Oh-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.271-279
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    • 2012
  • Total twenty cultivars of potato (Solanum tuberosum L.) were grown at Gangneung where is located in low land of Korea. Their tubers were harvested from 100 to 120 days after planting, and over 150 g of tubers were selected for quality evaluation. Dry matter, starch, amylose in starch, ascorbic acid, protein, individual sugars and mineral were analyzed and mealiness of cooked potatoes were also evaluated by panel test. Dry mater content was the highest in 'Haryeong', 'Jayoung' and 'Shepody', and the biggest starch granules were observed in 'Jayoung' and 'Sinnamjak'. In addition, the content of amylose was highest in 'Haryeong', 'Chudong' and 'Goun'. Mineral content showed the slight difference between cultivars, but it's tendency was not clear. Vitamin C content was highest in 'Jayoung' as 62.5 $mg{\cdot}100g^{-1}$ FW, and 'Chugang' exhibited the highest content of protein. Sugar content was lower in cultivars for single one than double cropping. The lowest sugar content was observed in 'Atlantic' and 'Namseo' among cultivars for single cropping, and in 'Goun' for double cropping. As a result of analysis for quality factors, we could select nine cultivars ('Atlantic', 'Gahwang', 'Gawon', 'Goun', 'Hareong', 'Irish Cobbler', 'Jasim', 'Jayoung' and 'Shepody') with high dry matter content and low reducing sugar as a cultivar group for processing. Additionally, seven cultivars ('Chugang', 'Gawon', 'Goun', 'Hareong', 'Irish Cobbler', 'Jasim' and 'Seohong') with high mealiness and amylose content and five cultivars ('Atlantic', 'Chudong', 'Gahwang', 'Jopung' and 'Jowon') with low mealiness and amylose content were classified as groups for boiled or steam cooking and for soup or pot dishes, respectively.