• Journal of the Korean earth science society
• /
• v.30 no.7
• /
• pp.810-823
• /
• 2009

One of the critical factors in accurate determination of earthquake source parameters, and in prediction of seismic hazards is the detailed information related to the site amplification characteristics. The site amplification characteristics of the broad-band seismic stations in Korea were estimated as a function of frequency in the range of 0.2 to 20 Hz. A total of 1275 seismograms recorded from 43 earthquakes observed from 2003 to 2008 in the southern Korean Peninsula were used. It was found that the site amplification ratios for 28 stations estimated from the inversion of the ground motion model were approximately concordant with those obtained from the horizontal-to-vertical (H/V) spectral ratio except for some stations. The spectral site amplification characteristics obtained in this study did not show any considerable spatial distribution. It revealed to be largely correlated with the degree of weathering rather than the basement rock type. Considering the spectral site amplification ratio, 28 broad-band stations were classified into four groups and the characteristics of each group were described in the text.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.24 no.3
• /
• pp.123-128
• /
• 2020

Analysis of the 2016 Gyeongju earthquake and the 2017 Pohang earthquake showed the characteristics of a typical high-frequency earthquake with many high-frequency components, short time strong motion duration, and large peak ground acceleration relative to the magnitude of the earthquake. Domestic nuclear power plants were designed and evaluated based on NRC's Regulatory Guide 1.60 design response spectrum, which had a great deal of energy in the low-frequency range. Therefore, nuclear power plants should carry out seismic verification and seismic performance evaluation of systems, structures, and components by reflecting the domestic characteristics of earthquakes. In this study, high-frequency amplification factors that can be used for seismic verification and seismic performance evaluation of nuclear power plant systems, structures, and equipment were analyzed. In order to analyze the high-frequency amplification factor, five sets of seismic time history were generated, which were matched with the uniform hazard response spectrum to reflect the characteristics of domestic earthquake motion. The nuclear power plant was subjected to seismic analysis for the construction of the Korean standard nuclear power plant, OPR1000, which is a reactor building, an auxiliary building assembly, a component cooling water heat exchanger building, and an essential service water building. Based on the results of the seismic analysis, a high-frequency amplification factor was derived upon the calculation of the floor response spectrum of the important locations of nuclear power plants. The high-frequency amplification factor can be effectively used for the seismic verification and seismic performance evaluation of electric equipment which are sensitive to high-frequency earthquakes.

• Parasites, Hosts and Diseases
• /
• v.51 no.2
• /
• pp.237-241
• /
• 2013

Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. The fecal samples were collected and prepared for microscopic analysis, and then the genomic DNA was extracted directly from purified cysts. The concentration of DNA samples of G. lamblia were diluted by 10-fold serially ranging from $10^{-1}$ to $10^{-5}ng/{\mu}l$ for LAMP and PCR assays. The LAMP assay allows the amplification to be finished within 60 min under isothermal conditions of $63^{\circ}C$ by employing 6 oligonucleotide primers designed based on G. lamblia elongation factor 1 alpha ($EF1{\alpha}$) gene sequence. Our tests showed that the specific amplification products were obtained only with G. lamblia, while no amplification products were detected with DNA of other related protozoans. Sensitivity evaluation indicated that the LAMP assay was sensitive 10 times more than PCR. It is concluded that LAMP is a rapid, highly sensitive and specific DNA amplification technique for detection of G. lamblia, which has implications for effective control and prevention of giardiasis.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.4
• /
• pp.1863-1869
• /
• 2014

The amplification of telomerase component (TERC) gene could play an important role in generation and treatment of haematological malignancies. This present study was aimed to investigate copy number amplification status of TERC gene in chronic myeloid leukaemia (CML) patients who were being treated with imatinib mesylate (IM). Genomic DNA was extracted from peripheral blood of CML-IM Resistant (n=63), CML-IM Respond (n=63) and healthy individuals (n=30). TERC gene copy number predicted (CNP) and copy number calculated (CNC) were determined based on $Taqman^{(R)}$ Copy Number Assay. Fluorescence in situ hybridization (FISH) analysis was performed to confirm the normal signal pattern in C4 (calibrator) for TERC gene. Nine of CML patients showed TERC gene amplification (CNP=3), others had 2 CNP. A total of 17 CML patients expressed CNC>2.31 and the rest had 2.31>CNC>1.5. TERC gene CNP value in healthy individuals was 2 and their CNC value showed in range 1.59-2.31. The average CNC TERC gene copy number was 2.07, 1.99 and 1.94 in CML-IM Resistant patients, CML-IM Respond and healthy groups, respectively. No significant difference of TERC gene amplification observed between CML-IM Resistant and CML-IM Respond patients. Low levels of TERC gene amplification might not have a huge impact in haematological disorders especially in terms of resistance towards IM treatment.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.8 no.5
• /
• pp.429-437
• /
• 2015

DDoS attacks have been used for paralyzing popular Internet services. Especially, amplification attacks have grown dramatically in recent years. Defending against amplification attacks is challenging since the attacks usually generate extremely hugh amount of traffic and attack traffic is coming from legitimate servers, which is hard to differentiate from normal traffic. Moreover, some of protocols used by amplification attacks are widely adopted in IoT devices so that the number of servers susceptible to amplification attacks will continue to increase. This paper studies on the analysis of amplification attack mechanisms in detail and proposes defense methodologies for scenarios where attackers, abused servers or victims are in a monitoring network.

• Steel and Composite Structures
• /
• v.37 no.1
• /
• pp.91-98
• /
• 2020

Steel plate shear walls are recently used as efficient seismic lateral resisting systems. These lateral resistant structures are implemented to provide more strength, stiffness and ductility in limited space areas. In this study, the seismic behavior of the multi-story steel frames with steel plate shear walls are investigated for buildings with 4, 8, 12 and 16 stories using verified computational modeling platforms. Different number of steel moment bays with distinctive lengths are investigated to effectively determine the deflection amplification factor for low-rise and high-rise structures. Results showed that the dissipated energy in moment frames with steel plates are significantly related to the inside panel. It is shown that more than 50% of the dissipated energy under various ground motions is dissipated by the panel itself, and increasing the steel plate length leads to higher energy dissipation capability. The deflection amplification factor is studied in details for various verified parametric cases, and it is concluded that for a typical multi-story moment frame with steel plate shear walls, the amplification factor is 4.93 which is less than the recommended conservative values in the design codes. It is shown that the deflection amplification factor decreases if the height of the building increases, for which the frames with more than six stories would have less recommended deflection amplification factor. In addition, increasing the number of bays or decreasing the steel plate shear wall length leads to a reduction of the deflection amplification factor.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.5
• /
• pp.662-667
• /
• 2020

Epidermal growth factor receptor (EGFR) mutations are not only genetic markers for diagnosis but also biomarkers of clinical-response against tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC). Among the EGFR mutations, the in-frame deletion mutation in EGFR exon 19 kinase domain (EGFR exon 19-del) is the most frequent mutation, accounting for about 45% of EGFR mutations in NSCLCs. Development of sensitive method for detecting the EGFR mutation is highly required to make a better screening for drug-response in the treatment of NSCLC patients. Here, we developed a fluorometric tandem gene amplification assay for sensitive detection of low-abundance EGFR exon 19-del mutant genomic DNA. The method consists of pre-amplification with PCR, thermal cycling of ligation by Taq ligase, and subsequent rolling circle amplification (RCA). PCR-amplified DNA from genomic DNA samples was used as splint DNA to conjugate both ends of linear padlock DNA, generating circular padlock DNA template for RCA. Long stretches of ssDNA harboring multiple copies of G-quadruplex structure was generated in RCA and detected by thioflavin T (ThT) fluorescence, which is specifically intercalated into the G-quadruplex, emitting strong fluorescence. Sensitivity of tandem gene amplification assay for detection of the EGFR exon 19-del from gDNA was as low as 3.6 pg, and mutant gDNA present in the pooled normal plasma was readily detected as low as 1% fraction. Hence, fluorometric detection of low-abundance EGFR exon 19 deletion mutation using tandem gene amplification may be applicable to clinical diagnosis of NSCLC patients with appropriate TKI treatment.

• The Journal of Engineering Geology
• /
• v.22 no.4
• /
• pp.399-408
• /
• 2012

To estimate seismic source and soil-structure interaction more reliably, site amplification characteristics should be considered. Among the various estimation methods, we used Nakamura's method (1989) to estimate site amplification. This method was originally applied to background noise; however, it has recently been successfully applied to S-wave and Coda-wave energy, and is applied to S-waves in the present study. We used more than 180 observed ground motions from 23 macro-earthquakes and then analyzed site amplification characteristics at eight seismic stations. Each station showed characteristics of site amplification properties in the low-, high- and resonance-frequency ranges. Comparison of the present results with those of other studies provide successful information regarding the dynamic amplification of domestic site characteristics and site classification.

• Korean Journal of Optics and Photonics
• /
• v.32 no.6
• /
• pp.306-313
• /
• 2021

We study the amplification properties of an optical amplifier based on a cesium-vapor cell. An optical amplification system including cesium vapor mixed with a buffer gas is built, and its amplification feature is investigated as a function of the size of the incident beam and the temperature of the cesium-vapor cell. We observe that the optical amplification properties, such as amplification factor and extraction efficiency, change significantly depending on the temperature and beam diameter of the pump and seed light. A maximum extraction efficiency of 56% is obtained when the temperature of the cesium cell is 90 ℃, with a 200-㎛ diameter of the pump (500 mW) and seed light (10 mW). The numerical simulation of the amplification properties agrees reasonably with the results obtained from the experiment.

• The Journal of Engineering Geology
• /
• v.33 no.2
• /
• pp.293-305
• /
• 2023

Three ground models are analyzed using a 1g shaking table and laminar shear box (LSB) to investigate the impact of the ground structure on seismic wave amplification during earthquakes. Multi-layer horizontal, embankment, and basin ground models are selected for this investigation, with each model being divided into dense and loose ground layers, Accelerometers are installed during the construction of each ground model to capture any seismic wave amplification owing th the propagation of an artificial seismic wave, sine wave sweep, and 10-Hz sine wave through a given ground model. The amplification of the tested seismic waves is analyzed using the observed peak ground acceleration and spectrum acceleration. The observed acceleration amplification in the multi-layer horizontal ground model is significantly higher the seismic waves that propagated across the dense ground-loose ground boundary compared with those that only propagated through the dense ground. Furthermore, the observed acceleration amplification gradually increases in the central part of the multi-layer embankment and basin models for the seismic waves that propagated across the dense ground-loose ground boundary.