• Proceedings of the Korean Society of Applied Pharmacology
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• 2003.11a
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• pp.110-110
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• 2003

It is well-documented that decreased antioxidant defense system by ultraviolet(UV) irradiation is the most important reason to induce the skin aging, especially photoaging. Chlorogenic acid(CA), a nonflavonoid catecholic compound, is present in the diet as part of fruits, tea, coffee and wine and has been reported to have anti-inflammatory, antimutagenic and anticarcinogenic activities. In this study, we examined the effects of CA on the UV -induced photoaging. Firstly, we investigated the protective effect of CA on antioxidant defense system in HaCaT human keratinocytes after UV irradiation treatment. UV irradiation decreased antioxidant defence enzyme activities of superoxide dismutase, catalase and GSH contents, which were restored by CA. To elucidate the effect of CA, 1% of CA and vehicle were applied to human buttock skin before and after UV irradiation (2MED). CA prevented UV -induced matrix metalloproteinase-1 mRNA expression and procollagen mRNA depression. And CA also increased CD1a(Langerhans cell) expression significantly. Our results suggest that CA has protective effects on UV -induced photoaging by increasing cellular antioxidant defense system. Therefore, CA may be a useful anti-aging agent for cosmetic purpose.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.2
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• pp.141-145
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• 2004

This study was pursued to measure skin feeling of cosmetics by mechanical methods. For this attempt, skin feeling of cosmetics such as spreading properties, tackiness, and residual greasy feeling after skin application was explored with the amount and kinds of cosmetic compositions-emulsifiers, waxes, thickeners, polyols, and oils. Furthermore, the relationship between these cosmetic compositions and viscometry of cosmetic products was studied. In case of emulsifiers, waxes, and thickeners, they showed strong correlation with both skin feeling and the value of phase angle, the property of viscometry, respectively, while polyols and oils were observed a special tendency neither skin feeling nor the property of viscometry. It leads to the conclusion that skin feeling may be corresponded to not values of a mechanical measure completely but a function of several properties. We expect that a better correlation can be discovered with additionally measured properties such as friction, volatility, etc.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.4 s.54
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• pp.285-287
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• 2005

Using optimized standard methods, which were previously done by sensory tests, many factors have been analyzed and their significance were determined according to gloss and gloss-lasting capability in lipstick. Analyzing some factors we could conclude that the effective factors were the kind of wax, amount of volatile silicone oil, and stick hardness in the aspect of lipstick's gloss. And in the point of lipstick's gloss-lasting properties, vital few X's were quantity of volatile silicone oil stick hardness, and polymer contents and so on. We successfully have tried new analytical approach, 6-sigma to general property of lipstick. Furthermore this study will be a base on the prescription design in gloss-enhanced products.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.191-192
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• 2002

Since capsaicin was found as an excellent vanilloid receptor agonist, considerable efforts toward the development of a novel analgesic have been continued. However, the small therapeutic window between these effects and the excitatory side effects, such as hypothermia, bronchoconstriction, increased GI mobility, and hypertension, precluded the development of capsaicin as a systemic agent. (omitted)

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.407-416
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• 2003

In this study, we evaluate the correlation between in vitro and in vivo determination of SPF of sunscreen products containing various ingredients depending on emulsification system. For in vitro approach, we determined SPF by the method of Diffey and Robson using an TransporeTM tape(3M Health care, USA) and SPF 290-analyzer(Optometrics Co. USA). SPF values and standard deviations are calculated and displayed after completion of the run. In vivo SPF values are determined according to KFDA (the Korea Food and Drug Administration) method in panels of Fitzpatrick's skin type II or III. We investigated the difference in SPF data of sunscreen ingredient according to emulsification system. The in vivo SPF data is high in water-in oil(W/O) emulsion than in oil-in water(O/W) emulsion samples. The difference may be due to the particular behavior in each vehicles and its presence on skin surface may produce a different sunscreen film. We obtained the corrlation coefficient between in vitro and in vivo SPF data for O/W (R-squre=0.72 )and W/O emulsion(R-squre=0.77). From these results, we suggest the improvement of methodology using Transpore$^{TM}$ tape as substrate to increase the predictability of in vitro method.d.

• Journal of Pharmaceutical Investigation
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• v.34 no.2
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• pp.101-106
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• 2004

This work aims at examining the cellular uptake behavior of poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles derivatized with a protein transduction domain (PTD) using HeLa cells. For this purpose, $Tat_{49-57}$ peptide derived from transcriptional activation (Tat) protein of HIV type-1 was covalently conjugated to the terminal end of PLGA. Nanoparticles were ten prepared with the $Tat_{49-57}-PLGA$ conjugates by a spontaneous phase inversion method. The prepared particles had a mean diameter of ca. 84 nm, as measured by dynamic light scattering. The interaction of the Tat-PLGA nanoparticles with cells was examined by using confocal laser scanning microscopy. It was found tat Tat-PLGA nanoparticles incubated with HeLa cells could efficiently translocate into cytoplasm, while plain PLGA nanoparticles showed negligible cellular uptake. In addition, even at $4^{\circ}C$ or in the presence of sodium azide significant cellular internalization of Tat-PLGA nanoparticles was still observed. These results indicate that a non-endocytotic translocation mechanism might be involved in the cellular uptake of Tat-PLGA nanoparticles.

• Bulletin of the Korean Chemical Society
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• v.31 no.8
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• pp.2375-2378
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• 2010

• Bulletin of the Korean Chemical Society
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• v.33 no.4
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• pp.1333-1336
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• 2012

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.349.1-349.1
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• 2002

Capsaicin. the pungent component of chili pepper. opens a novel cation selective ion channel in the plasma membrane of peripheral sensory neurons. Capsaicin channel agonists induce pain upon topical application in the early stage. which is followed by a period of desensitization. Although the agonists have been studied as a analgesics, their initial irritancy became sever side effect. So competive antagonists have been pursued as a novel pharmacological agent for analgesics, rather than agonists. (omitted)

• Toxicological Research
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• v.35 no.4
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• pp.389-394
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• 2019

When conducting risk assessments of cosmetic ingredients, it is important that reliable exposure information is obtained for cosmetic products. As cosmetics are becoming more diverse, continuous effort must be made to obtain exposure data that reflect their growth and usage trends. The usage pattern of cosmetics, such as the application area and amount used, may differ by product type and also by country. We conducted a survey to compare the amount of sun spray and sun cream used in a usage environment in South Korea. The study was conducted on Haeundae Beach, one of the most popular beaches in South Korea. A total of 1,255 beachgoers participated in this study; 604 and 651 participants used the sun spray and sun cream, respectively, while sunbathing and enjoying water activities on the beach for one day. Exposure was analyzed following a probabilistic method. On comparing all subjects, it was found that the group that used sun spray (mean: 44.52 g/day) used significantly more product (p = 0.000) than those who used sun cream (mean: 20.51 g/day). By analyzing the daily exposure of sun spray and sun cream per unit body weight according to age and gender, the exposure amount of sun spray and sun cream was found to be highest among 2~9 year-old girls (mean for sun spray: 2.51 g/kg/day, p95: 5.50 g/kg/day, mean for sun cream: 0.79 g/kg/day, p95: 1.79 g/kg/day). The amount of sun spray used is approximately twice that of sun cream. Among both the sun spray and sun cream groups, the exposure amount per unit body weight was highest in girls younger than 10. These factors should be considered when conducting risk assessments of sun spray and sun cream.