• Korean Journal of Microbiology
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• v.55 no.3
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• pp.283-285
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• 2019

The draft genome sequencing for Pelagicola sp. DSW4-44 (= KCTC 62762 = KCCM 43261), isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.85 Mbp with G + C content of 54.3%, and included a total of 4,566 protein-coding genes, 3 rRNA genes, 48 tRNA genes, 3 non-coding RNA genes, and 67 pseudo genes. In the draft genome, the strain DSW4-44 contained genes involved in the nitrogen metabolism of dissimilatory nitrate reduction to ammonium (DNRA) and denitrification, which were not found other strains in the genus Pelagicola.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.696-704
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• 2021

Levan is an industrially important, functional biopolymer with considerable applications in the food and pharmaceutical fields owing to its safety and biocompatibility. Here, levan-type exopolysaccharide produced by Pantoea agglomerans ZMR7 was purified by cold ethanol precipitation and characterized using TLC, FTIR, ¹H, and ¹³C NMR spectroscopy. The maximum production of levan (28.4 g/l) was achieved when sucrose and ammonium chloride were used as carbon and nitrogen sources, respectively, at 35℃ and an initial pH of 8.0. Some biomedical applications of levan like antitumor, antiparasitic, and antioxidant activities were investigated in vitro. The results revealed the ability of levan at different concentrations to decrease the viability of rhabdomyosarcoma and breast cancer cells compared with untreated cancer cells. Levan appeared also to have high antiparasitic activity against the promastigote of Leishmania tropica. Furthermore, levan had strong DPPH radical scavenging (antioxidant) activity. These findings suggest that levan produced by P. agglomerans ZMR7 can serve as a natural biopolymer candidate for the pharmaceutical and medical fields.

• The Korean Journal of Mycology
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• v.7 no.1
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• pp.13-73
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• 1979

These studies were conducted to investigate nutrient sources and supplementary materials of synthetic compost media for Agaricus bisporus culture. Investigation were carried out to establish the optimum composition for compost of Agaricus bisporus methods of out-door fermentation and peakheating with rice straw as the main substrate of the media. The incidence and flora of harmful organisms in rice straw compost and their control were also studied. 1. When rice straw was used as the main substrate in synthetic compost as a carbon source. yields were remarkably high. Fermentation was more rapid than that of barley straw or wheat straw, and the total nitrogen content was high in rice straw compost. 2. Since the morphological and physico-chemical nature of Japonica and Indica types of rice straw are greatly dissimilar. there were apparent differences in the process of compost fermentation. Fermentation of Indica type straw proceeded more rapidly with a shortening the compost period, reducing the water supply, and required adding of supplementary materials for producing stable physical conditions. 3. Use of barley straw compost resulted in a smaller crop compared with rice straw. but when a 50%, barley straw and 50% rice straw mixture was used, the yield was almost the same as that using only rice straw. 4. There were extremely high positive correlations between yield of Agaricus bisporus and the total nitrogen, organic nitrogen, amino acids, amides and amino sugar nitrogen content of compost. The mycerial growth and fruit body formation were severely inhibited by ammonium nitrogen. 5. When rice straw was used as the main substrate for compost media, urea was the most suitable source of nitrogen. Poor results were obtained with calcium cyanamide and ammonium sulfate. When urea was applied three separate times, nitrogen loss during composting was decreased and the total nitrogen content of compost was increased. 6. The supplementation of organic nutrient activated compost fermentation and increased yield of Agaricus bisporus. The best sources of organic nutrients were: perilla meal, sesame meal, wheat bran and poultry manure, etc. 7. Soybean meal, tobacco powder and glutamic acid fermentation by-products which were industrial wastes, could be substituted for perilla meal, sesame meal and wheat bran as organic nutrient sources for compost media. B. When gypsum and zeolite were added to rice straw. physical deterioration of compost due to excess moisture and caramelization was observed. The Indica type of straw was more remarkable in increase of yield of Agricus bisporus by addition of supplementing materials than Japonica straw. 9. For preparing rice straw compost, the best mixture was prepared by 10% poultry manure, 5% perilla meal, 1. 2 to 1. 5% urea and 1% gypsum. At spring cropping, it was good to add rice bran to accelerate heat generation of the compost heap. 10. There was significantly high positive correlation (r=0.97) between accumulated temperature and the decomposition degree of compost during outdoor composting. The yield was highest at accumulated temperatures between 900 and $1,000^{\circ}C$. 11. Prolonging the composting period brought about an increase in decomposition degree and total nitrogen content, but a decrease in ammonium nitrogen. In the spring the suitable period of composting was 20 to 25 days. and about 15 days in autumn. For those periods, the degree of decomposition was 19 to 24%. 12. Compactness of wet compost at filling caused an increase in the residual ammonium nitrogen. methane and organic acid during peak heating. There was negative correlation between methane content and yield (r=0.76)and the same was true between volatile organic acid and yield (r=0.73). 13. In compost with a moisture content range between 69 to 80% at filling. the higher the moisture content, the lower the yield (r=0.78). This result was attributed to a reduction in the porosity of compost at filling the beds. The optimum porosity for good fermentation was between 41 and 53%. 14. Peak heating of the compost was essential for the prevention of harmful microorganisms and insect pests. and for the removal of excess ammonia. It was necessary to continue fer mentatiion for four days after peak heating. 15. Ten species of fungi which are harmful or competitive to Agaricus bisporus were identified from the rice compost, including Diehliomyces microsporus, Trichoderma sp. and Stysanus stemoites. The frequency of occurrance was notably high with serious damage to Agaricus bisporus. 16. Diehliomyces microsporus could be controlled by temperature adjustment of the growing room and by fumigating the compost and the house with Basamid and Vapam. Trichoderma was prevented by the use of Bavistin and Benomyl. 17. Four species of nematodes and five species of mites occured in compost during out-door composting. These orgnanisms could be controlled through peakheating compost for 6 hours at $60^{\circ}C$.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.2
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• pp.107-113
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• 1987

A pot experiment was carried out using Seomjinbyeo to investigate the effects of some sulfur-containing substances on the growth of rice plants and on soil characteristics. The results are summarized as follows: 1. Soil pH in plots given the sulfur treatments was lower than that in the urea plot during the growing stages. Soil pH in plots given the mineral sulfur treatments was higher than that in the ammonium sulfate plot at the early growth stage of rice. However, as the stage of rice growth progressed, soil pH became similar. After the experiment, soil pH was higher, but the sulfur content of soil was lower in the urea plot compared with that in other sulfur plots. After the experiment, the sulfur content of soil in the ammonium sulfate plot was the lowest among the plots given the sulfur treatments. 2. Plant heights and the number of tillers of the rice plants in plots given the sulfur treatments were higher than those in the urea plot, specially in the number of tillers of the rice plants. 3. Nitrogen and sulfur contents of the tops of rice plants in plots given the sulfur treatments were slightly higher than those in the urea plot. nitrogen and sulfur contents in the ammonium sulfate plot were higher than those in plots given the mineral sulfur treatments at the early growth stage of rice. However, as the stage of rice growth progressed, those in plots given the mineral sulfur treatments were higher. 4. The chlorophyll content in the leaf blade at heading stage in plots given the gypsume and given the mineral sulfur treatments was significantly higher than that in the urea plot. The N/S ratio was negatively correlated with the chlorophyll content.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.111-122
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• 2009

Compound fertilizer contained slow release nitrogen was used at golf course management because of promoting turfgrass growth and decreasing fertilizing labor. But, it was hardly published about turf grass growth by its effect and form like isobutylidene diurea(IBDU) and methylene urea(MU) in golf course management of Korea. This study was conducted to investigate the effect of compound fertilizer contained slow release nitrogen such as IBDU and MU on creeping bentgrasss and the change in nitrogen in the root zone after application of fertilizers at AM turfgrass research institute with soil analysis, chlorophyll content index, leaf area index and dry weight during 5 months from July to december in 2007 year. Fertilizer treatments were designed as following; non-fertilizer(NF), straight fertilizer(CF), treatment 1(MU), treatment 2(IBDU 1), and treatment 3(IBDU 2). The every treatments were arranged a randomized complete block design with three replications. Results obtained were summarized as follows: As relative to time after N fertilizers application, ammonium and nitrate in soil were increased, but T-N was decrease. $NO_3$-N and T-N content of slow release fertilizer(SRF), the treatment MU, IBDU 1 and IBDU 2, in soil was higher than that of CF, and the change of ratio of available N and T-N by elapsed time after fertilizing SRFs and CF was higher about 5% in root zone of SF to l5days, but in root zone of SRF from 30days about $5{\sim}10%$. Compared with NF, the turf color index was increased 6.5%, 6.7%, 5.9% and 5.5%, respectively, the chlorophyll content 33.2%, 38.4%, 35.1% and 37.1%, the dry weight 76.2%, 77.7%, 69.5 and 72.3% and shoot number 28%, 52%, 56% and 51%. The chlorophyll content index of MD, IBDU 1 and IBDU 2 was increased $2.6{\sim}5.2%$ than that of CF, shoot number $18{\sim}22%$ but turf color index and dry weight was analogous to that of CF. These results showed that the SRF application was supplied nitrogen for a long time in root zone by increasing an available nitrogen, and so bring turf growth such as chlorophyll content and shoot number in creeping bentgrass.

• Korean Journal of Soil Science and Fertilizer
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• v.21 no.2
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• pp.177-181
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• 1988

To get the basic information about ginseng seedling production, yield of ginseng seedling and soil physico-chemical components of Ban-Yang-Jik (semimodified soil) nursery in 29 farmer's field were investigated. The number of available seedling per Kan (Kan means $180{\times}90cm$ area) is $362{\pm}226$. Root weight per seedling was negatively correlated with ammount of fine and extremely fine sand. Positive correlations were shown between pH and OM, K, Ca and Mg, and also between EC and Ca, Mg and ammonium and nitrate nitrogen in soil of nursery. There were significant linear relations between root weight and OM, K, Ca and Mg in soil of nursery. On the other hand, quadratic relation was held between the root weight and $P_2O_5$, but the root weight has no correlation with nitrogen. The nitrogen contents of soil might not influence on the growth of ginseng seedling as greatly as those of $P_2O_5$, K and Ca. The contents of $P_2O_5$, K and Ca in root were increased with increase of the contents of $P_2O_5$, K and Ca in soil of nusery, respectively. It showed the linear correlation between the root weight and $P_2O_5$ and Ca, otherwise quadratic correlation between the root weight and K in root.

• KSBB Journal
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• v.14 no.1
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• pp.36-44
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• 1999

The biosynthesis of Lovastatin, a cholesterol lowering agent formed by the filamentous fungus Aspergillus terreus, was investigated in shaking flask. The effects of essential elements in the experimental medium such as carbon, nitrogen, phosphate sources, and amino acids were examined to increase Lovastatin productivity. Lovastatin production in shaking flasks was 68 mg/L in the used medium. Effect of carbon source on Lovastatin production was performed. As a carbon source in the medium, 45 mL/L of glycerol increased the Lovastatin production up to 256 mg/L, which was found to be improved almost 3.5 times in comparison with that in common medium. The optimum concventration of peptonized milk as nitrogen source was obtained 30g/L on Lovastatin production. The severe inhibition of the cell growth and the Lovastatin production were observed in shaking flasks conducted at the medium contained ammonium carbonate as a nitrogen source. Lovastatin production various concentrations of several phosphate compounds was also examined. The addition of either potassium phosphate diabsic or sodium phosphate dibasic increased the Lovastatin production and the optimal level of potassium phosphate dibasic was 6 g/L. Even though Lovastatin contain methionine-derived methyl group, L-methionine and DL-methionine tend to diminish the Lovastatin production. Among the amino acids, L-histidine and L-tryptophan had a remarkable enhancing effect on the Lovastatin production. The optimal concentration of L-histidine and L-tryptophan was 6g/L.

• Journal of Wetlands Research
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• v.15 no.2
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• pp.215-222
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• 2013

In this study, the nutrient concentration changes along the hydrologic flow path of a free water surface flow constructed wetland (CW) treating agricultural stream runoff was investigated. Dry sampling was performed from April 2009 to November 2011 at five locations representing each treatment units of the CW. Grab water samples were analyzed for nitrogen forms such as total nitrogen (TN), total Kjeldahl nitrogen, nitrate, and ammonium; and phosphorus forms including total phosphorus (TP) and phosphate. Findings revealed that the physical properties such as temperature, dissolved oxygen and pH affected the TP retention in the CW. High nutrient reduction was observed after passing the first sedimentation zone indicating the importance of settling process in the retention of nutrients. However, it was until the 85% of the length of the CW where nutrient retention was greatest indicating the deposition of nutrients at the alternating shallow and deep marshes. TN and TP concentration seemed to increase at the final sedimentation zone (FSZ) suggesting a possible nutrient source in this segment of the CW. It was therefore recommended to reduce or possibly remove the FSZ in the CW for an optimum performance, smaller spatial allocation and lesser construction expenses for similar systems.

• Korean Journal of Microbiology
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• v.47 no.3
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• pp.249-254
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• 2011

Mycophenolic acid blocking the synthesis of xanthosine monophosphate is a nonnucleoside inhibitor of inosine monophosphate dehydrogenase. Therefore mycopholoic acid is a drug currently used as immunosuppressive agent in transplantation of heart, kidney and liver. Mycophenolic acid has been industrially produced through fermentation process by fungus Penicillium brevi-compactum. In this study, the profile of mycophenolic acid fermentation was observed in 5L-jar fermentor to investigate the utilization of carbon and nitrogen sources and the production of mycophenolic acid. It was investigated that what kind of carbon sources was better to cell growth and mycophenolic acid production. Fructose was the best carbon source for mycophenolic acid fermentation, but it is the most expensive one. Thereafter molasses containing sucrose as the supply source of fructose was confirmed to be the best carbon source for the industrial production. Use of molasses increased the fermentation yield of mycophenolic acid more than two times higher than glucose. It was confirmed that urea was the best inorganic nitrogen source, which did not give rise to sudden drop of culture pH. Addition of urea increased the fermentation yield of mycophenolic acid about 3.6 times higher than addition of ammonium nitrate as control. Casein, peptone and casamino acid originated from milk protein increased the fermentation yield of mycophenolic acid about 3.4 times higher than control. Peptone and casamino acid, which are casein hydrolysates, increased cell growth considerably as well.

• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.4
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• pp.32-39
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• 2015

Objective of this study was to investigate characteristics of inorganic components contained in liquid fertilizer produced using seaweed by adding microorganisms and molasses. Addition of dry yeast to liquid fertilizer resulted in little change in pH and considerable increase in EC with high EC value compared to other liquid fertilizers which have microorganisms additives. Also, it was appeared that the dry yeast-added treatment had higher $NH_4-N$ concentration than other treatments. In the other hand, addition of molasses resulted in low pH compared to the control which has no additives, and EC was not different depending on the amount of molasses. $NH_4-N$ concentration in the 2% molasses added treatment was lowest and it showed a significant difference in the no and 1% molasses added treatments. In conclusion, it was shown that addition of dry yeast to liquid fertilizer increased ammonium nitrogen concentration by accelerating nitrogen mineralization, while molasses has an effect of inhibiting nitrogen mineralization. With application of organic liquid fertilizer containing seaweed increased the fresh weight of chinese cabbage.